Experiment: SJC25_Acapsular_0.1MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2277 and BT2278 are separated by 46 nucleotides BT2278 and BT2279 are separated by 101 nucleotides BT2279 and BT2280 are separated by 1 nucleotides BT2280 and BT2281 are separated by 186 nucleotides
BT2277: BT2277 - transposase (NCBI ptt file), at 2,857,852 to 2,859,111
BT2277
BT2278: BT2278 - conserved hypothetical protein (NCBI ptt file), at 2,859,158 to 2,859,571
BT2278
BT2279: BT2279 - putative ribose phosphate pyrophosphokinase (NCBI ptt file), at 2,859,673 to 2,860,593
BT2279
BT2280: BT2280 - hypothetical protein (NCBI ptt file), at 2,860,595 to 2,860,969
BT2280
BT2281: BT2281 - hypothetical protein (NCBI ptt file), at 2,861,156 to 2,861,428
BT2281
Position (kb)
2859
2860
2861 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2858.730 kb on + strand, within BT2277 at 2858.769 kb on - strand, within BT2277 at 2858.854 kb on + strand, within BT2277 at 2858.902 kb on + strand, within BT2277 at 2859.054 kb on + strand at 2859.107 kb on + strand at 2859.107 kb on + strand at 2859.114 kb on - strand at 2859.126 kb on - strand at 2859.127 kb on + strand at 2859.128 kb on - strand at 2859.128 kb on - strand at 2859.142 kb on + strand at 2859.166 kb on + strand at 2859.225 kb on + strand, within BT2278 at 2859.246 kb on + strand, within BT2278 at 2859.246 kb on + strand, within BT2278 at 2859.300 kb on + strand, within BT2278 at 2859.301 kb on - strand, within BT2278 at 2859.388 kb on + strand, within BT2278 at 2859.392 kb on + strand, within BT2278 at 2859.395 kb on + strand, within BT2278 at 2859.398 kb on - strand, within BT2278 at 2859.414 kb on + strand, within BT2278 at 2859.414 kb on + strand, within BT2278 at 2859.415 kb on - strand, within BT2278 at 2859.440 kb on + strand, within BT2278 at 2859.440 kb on + strand, within BT2278 at 2859.553 kb on + strand at 2859.563 kb on + strand at 2859.565 kb on + strand at 2859.579 kb on - strand at 2859.664 kb on - strand at 2859.678 kb on - strand at 2859.736 kb on + strand at 2859.792 kb on + strand, within BT2279 at 2859.799 kb on + strand, within BT2279 at 2859.800 kb on - strand, within BT2279 at 2859.857 kb on - strand, within BT2279 at 2859.858 kb on + strand, within BT2279 at 2859.858 kb on + strand, within BT2279 at 2859.858 kb on + strand, within BT2279 at 2859.863 kb on + strand, within BT2279 at 2859.864 kb on - strand, within BT2279 at 2859.915 kb on + strand, within BT2279 at 2859.925 kb on - strand, within BT2279 at 2859.981 kb on + strand, within BT2279 at 2859.981 kb on + strand, within BT2279 at 2859.982 kb on - strand, within BT2279 at 2859.984 kb on - strand, within BT2279 at 2859.984 kb on - strand, within BT2279 at 2859.994 kb on - strand, within BT2279 at 2860.030 kb on + strand, within BT2279 at 2860.081 kb on + strand, within BT2279 at 2860.082 kb on - strand, within BT2279 at 2860.093 kb on + strand, within BT2279 at 2860.152 kb on - strand, within BT2279 at 2860.156 kb on - strand, within BT2279 at 2860.161 kb on + strand, within BT2279 at 2860.466 kb on + strand, within BT2279 at 2860.479 kb on + strand, within BT2279 at 2860.479 kb on + strand, within BT2279 at 2860.668 kb on + strand, within BT2280 at 2860.668 kb on + strand, within BT2280 at 2860.669 kb on - strand, within BT2280 at 2860.810 kb on - strand, within BT2280 at 2860.856 kb on - strand, within BT2280 at 2860.882 kb on - strand, within BT2280 at 2860.912 kb on + strand, within BT2280 at 2860.979 kb on + strand at 2860.980 kb on - strand at 2860.980 kb on - strand at 2860.980 kb on - strand at 2860.982 kb on + strand at 2860.982 kb on + strand at 2861.036 kb on + strand at 2861.059 kb on + strand at 2861.093 kb on + strand at 2861.093 kb on + strand at 2861.094 kb on - strand at 2861.094 kb on - strand at 2861.142 kb on - strand at 2861.142 kb on - strand at 2861.182 kb on + strand at 2861.182 kb on + strand at 2861.186 kb on + strand, within BT2281 at 2861.197 kb on - strand, within BT2281 at 2861.304 kb on - strand, within BT2281 at 2861.342 kb on - strand, within BT2281 at 2861.367 kb on + strand, within BT2281 at 2861.367 kb on + strand, within BT2281 at 2861.428 kb on + strand at 2861.440 kb on + strand at 2861.441 kb on - strand at 2861.444 kb on - strand at 2861.448 kb on + strand at 2861.521 kb on + strand at 2861.536 kb on - strand at 2861.536 kb on - strand at 2861.536 kb on - strand at 2861.536 kb on - strand at 2861.536 kb on - strand at 2861.561 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SJC25_Acapsular_0.1MOI remove 2,858,730 + BT2277 0.70 +0.5 2,858,769 - BT2277 0.73 -0.2 2,858,854 + BT2277 0.80 +0.2 2,858,902 + BT2277 0.83 -0.2 2,859,054 + -0.8 2,859,107 + -0.2 2,859,107 + -0.2 2,859,114 - -0.7 2,859,126 - -1.0 2,859,127 + +1.2 2,859,128 - -2.2 2,859,128 - +0.9 2,859,142 + -0.1 2,859,166 + +0.5 2,859,225 + BT2278 0.16 +1.3 2,859,246 + BT2278 0.21 -1.7 2,859,246 + BT2278 0.21 -1.7 2,859,300 + BT2278 0.34 -1.0 2,859,301 - BT2278 0.35 -0.3 2,859,388 + BT2278 0.56 -2.5 2,859,392 + BT2278 0.57 -0.7 2,859,395 + BT2278 0.57 +1.8 2,859,398 - BT2278 0.58 -0.1 2,859,414 + BT2278 0.62 -0.7 2,859,414 + BT2278 0.62 -0.5 2,859,415 - BT2278 0.62 +1.7 2,859,440 + BT2278 0.68 -0.7 2,859,440 + BT2278 0.68 -0.8 2,859,553 + -0.1 2,859,563 + +2.1 2,859,565 + -0.7 2,859,579 - +0.0 2,859,664 - -0.4 2,859,678 - -0.9 2,859,736 + -1.9 2,859,792 + BT2279 0.13 -1.7 2,859,799 + BT2279 0.14 +0.9 2,859,800 - BT2279 0.14 -2.2 2,859,857 - BT2279 0.20 -3.0 2,859,858 + BT2279 0.20 +1.6 2,859,858 + BT2279 0.20 +1.6 2,859,858 + BT2279 0.20 -0.9 2,859,863 + BT2279 0.21 -0.9 2,859,864 - BT2279 0.21 -2.6 2,859,915 + BT2279 0.26 -0.7 2,859,925 - BT2279 0.27 -1.1 2,859,981 + BT2279 0.33 +0.3 2,859,981 + BT2279 0.33 +1.2 2,859,982 - BT2279 0.34 +1.5 2,859,984 - BT2279 0.34 -1.2 2,859,984 - BT2279 0.34 -0.7 2,859,994 - BT2279 0.35 -1.5 2,860,030 + BT2279 0.39 +0.5 2,860,081 + BT2279 0.44 +0.9 2,860,082 - BT2279 0.44 +0.1 2,860,093 + BT2279 0.46 -1.6 2,860,152 - BT2279 0.52 -1.4 2,860,156 - BT2279 0.52 -0.1 2,860,161 + BT2279 0.53 -1.5 2,860,466 + BT2279 0.86 +2.9 2,860,479 + BT2279 0.88 -1.4 2,860,479 + BT2279 0.88 -2.7 2,860,668 + BT2280 0.19 -1.7 2,860,668 + BT2280 0.19 -2.7 2,860,669 - BT2280 0.20 -0.1 2,860,810 - BT2280 0.57 -1.9 2,860,856 - BT2280 0.70 -0.2 2,860,882 - BT2280 0.77 +0.9 2,860,912 + BT2280 0.85 -1.9 2,860,979 + -0.1 2,860,980 - -3.4 2,860,980 - +0.5 2,860,980 - +0.9 2,860,982 + -0.9 2,860,982 + -3.8 2,861,036 + -0.4 2,861,059 + -0.1 2,861,093 + +1.5 2,861,093 + -3.4 2,861,094 - +0.6 2,861,094 - -0.1 2,861,142 - -0.9 2,861,142 - -1.1 2,861,182 + -0.7 2,861,182 + -1.7 2,861,186 + BT2281 0.11 +1.5 2,861,197 - BT2281 0.15 +0.3 2,861,304 - BT2281 0.54 +1.4 2,861,342 - BT2281 0.68 +1.3 2,861,367 + BT2281 0.77 -0.7 2,861,367 + BT2281 0.77 +0.9 2,861,428 + -0.6 2,861,440 + -0.7 2,861,441 - -0.4 2,861,444 - -0.7 2,861,448 + -1.3 2,861,521 + +0.2 2,861,536 - -0.7 2,861,536 - +2.7 2,861,536 - -0.1 2,861,536 - +0.1 2,861,536 - +1.5 2,861,561 + -1.2
Or see this region's nucleotide sequence