Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS09620

Experiment: Choline chloride 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS09610 and PFLU_RS09615 are separated by 11 nucleotidesPFLU_RS09615 and PFLU_RS09620 are separated by 88 nucleotidesPFLU_RS09620 and PFLU_RS09625 are separated by 5 nucleotides PFLU_RS09610: PFLU_RS09610 - histidinol dehydrogenase, at 2,122,639 to 2,123,922 _RS09610 PFLU_RS09615: PFLU_RS09615 - SDR family oxidoreductase, at 2,123,934 to 2,124,686 _RS09615 PFLU_RS09620: PFLU_RS09620 - 2-keto-4-pentenoate hydratase, at 2,124,775 to 2,125,755 _RS09620 PFLU_RS09625: PFLU_RS09625 - NAD-dependent epimerase/dehydratase family protein, at 2,125,761 to 2,126,702 _RS09625 Position (kb) 2124 2125 2126Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2123.788 kb on + strand, within PFLU_RS09610at 2123.788 kb on + strand, within PFLU_RS09610at 2123.788 kb on + strand, within PFLU_RS09610at 2123.788 kb on + strand, within PFLU_RS09610at 2123.789 kb on - strand, within PFLU_RS09610at 2123.789 kb on - strand, within PFLU_RS09610at 2123.789 kb on - strand, within PFLU_RS09610at 2123.789 kb on - strand, within PFLU_RS09610at 2123.789 kb on - strand, within PFLU_RS09610at 2123.789 kb on - strand, within PFLU_RS09610at 2123.789 kb on - strand, within PFLU_RS09610at 2123.789 kb on - strand, within PFLU_RS09610at 2123.789 kb on - strand, within PFLU_RS09610at 2123.789 kb on - strand, within PFLU_RS09610at 2123.789 kb on - strand, within PFLU_RS09610at 2124.032 kb on - strand, within PFLU_RS09615at 2124.042 kb on + strand, within PFLU_RS09615at 2124.043 kb on - strand, within PFLU_RS09615at 2124.043 kb on - strand, within PFLU_RS09615at 2124.222 kb on + strand, within PFLU_RS09615at 2124.222 kb on + strand, within PFLU_RS09615at 2124.222 kb on + strand, within PFLU_RS09615at 2124.223 kb on - strand, within PFLU_RS09615at 2124.223 kb on - strand, within PFLU_RS09615at 2124.329 kb on - strand, within PFLU_RS09615at 2124.363 kb on - strand, within PFLU_RS09615at 2124.363 kb on - strand, within PFLU_RS09615at 2124.408 kb on + strand, within PFLU_RS09615at 2124.409 kb on - strand, within PFLU_RS09615at 2124.684 kb on + strandat 2124.685 kb on - strandat 2124.878 kb on + strand, within PFLU_RS09620at 2124.879 kb on - strand, within PFLU_RS09620at 2124.879 kb on - strand, within PFLU_RS09620at 2124.932 kb on - strand, within PFLU_RS09620at 2124.932 kb on - strand, within PFLU_RS09620at 2125.012 kb on + strand, within PFLU_RS09620at 2125.012 kb on + strand, within PFLU_RS09620at 2125.070 kb on - strand, within PFLU_RS09620at 2125.070 kb on - strand, within PFLU_RS09620at 2125.467 kb on + strand, within PFLU_RS09620at 2125.467 kb on + strand, within PFLU_RS09620at 2125.467 kb on + strand, within PFLU_RS09620at 2125.468 kb on - strand, within PFLU_RS09620at 2125.561 kb on - strand, within PFLU_RS09620at 2125.589 kb on + strand, within PFLU_RS09620at 2125.589 kb on + strand, within PFLU_RS09620at 2125.590 kb on - strand, within PFLU_RS09620at 2125.590 kb on - strand, within PFLU_RS09620at 2125.590 kb on - strand, within PFLU_RS09620at 2125.952 kb on + strand, within PFLU_RS09625at 2126.022 kb on + strand, within PFLU_RS09625at 2126.022 kb on + strand, within PFLU_RS09625at 2126.023 kb on - strand, within PFLU_RS09625at 2126.023 kb on - strand, within PFLU_RS09625at 2126.191 kb on - strand, within PFLU_RS09625at 2126.191 kb on - strand, within PFLU_RS09625at 2126.541 kb on + strand, within PFLU_RS09625at 2126.542 kb on - strand, within PFLU_RS09625at 2126.542 kb on - strand, within PFLU_RS09625at 2126.611 kb on - strandat 2126.707 kb on + strandat 2126.707 kb on + strandat 2126.737 kb on - strandat 2126.737 kb on - strandat 2126.737 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline chloride 10 mM (C)
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2,123,788 + PFLU_RS09610 0.89 +0.1
2,123,788 + PFLU_RS09610 0.89 -3.0
2,123,788 + PFLU_RS09610 0.89 +0.3
2,123,788 + PFLU_RS09610 0.89 -0.1
2,123,789 - PFLU_RS09610 0.90 +0.1
2,123,789 - PFLU_RS09610 0.90 +0.8
2,123,789 - PFLU_RS09610 0.90 +0.0
2,123,789 - PFLU_RS09610 0.90 -3.5
2,123,789 - PFLU_RS09610 0.90 +2.0
2,123,789 - PFLU_RS09610 0.90 +0.8
2,123,789 - PFLU_RS09610 0.90 -2.1
2,123,789 - PFLU_RS09610 0.90 -0.3
2,123,789 - PFLU_RS09610 0.90 +0.1
2,123,789 - PFLU_RS09610 0.90 -2.7
2,123,789 - PFLU_RS09610 0.90 +1.7
2,124,032 - PFLU_RS09615 0.13 +0.2
2,124,042 + PFLU_RS09615 0.14 -2.4
2,124,043 - PFLU_RS09615 0.14 -1.1
2,124,043 - PFLU_RS09615 0.14 -0.8
2,124,222 + PFLU_RS09615 0.38 -0.4
2,124,222 + PFLU_RS09615 0.38 -2.4
2,124,222 + PFLU_RS09615 0.38 +0.4
2,124,223 - PFLU_RS09615 0.38 +0.2
2,124,223 - PFLU_RS09615 0.38 -1.9
2,124,329 - PFLU_RS09615 0.52 -1.7
2,124,363 - PFLU_RS09615 0.57 -1.3
2,124,363 - PFLU_RS09615 0.57 -1.1
2,124,408 + PFLU_RS09615 0.63 -0.9
2,124,409 - PFLU_RS09615 0.63 +1.1
2,124,684 + +0.7
2,124,685 - -2.6
2,124,878 + PFLU_RS09620 0.10 +4.0
2,124,879 - PFLU_RS09620 0.11 +2.4
2,124,879 - PFLU_RS09620 0.11 -0.9
2,124,932 - PFLU_RS09620 0.16 -2.0
2,124,932 - PFLU_RS09620 0.16 -2.2
2,125,012 + PFLU_RS09620 0.24 -1.6
2,125,012 + PFLU_RS09620 0.24 +1.4
2,125,070 - PFLU_RS09620 0.30 +2.1
2,125,070 - PFLU_RS09620 0.30 +0.3
2,125,467 + PFLU_RS09620 0.71 +0.7
2,125,467 + PFLU_RS09620 0.71 +2.5
2,125,467 + PFLU_RS09620 0.71 -0.4
2,125,468 - PFLU_RS09620 0.71 +1.2
2,125,561 - PFLU_RS09620 0.80 +0.9
2,125,589 + PFLU_RS09620 0.83 +1.7
2,125,589 + PFLU_RS09620 0.83 -1.3
2,125,590 - PFLU_RS09620 0.83 -2.2
2,125,590 - PFLU_RS09620 0.83 +1.7
2,125,590 - PFLU_RS09620 0.83 +1.9
2,125,952 + PFLU_RS09625 0.20 +1.1
2,126,022 + PFLU_RS09625 0.28 +0.9
2,126,022 + PFLU_RS09625 0.28 +1.8
2,126,023 - PFLU_RS09625 0.28 +0.1
2,126,023 - PFLU_RS09625 0.28 +1.2
2,126,191 - PFLU_RS09625 0.46 -1.7
2,126,191 - PFLU_RS09625 0.46 -2.6
2,126,541 + PFLU_RS09625 0.83 +1.3
2,126,542 - PFLU_RS09625 0.83 -0.3
2,126,542 - PFLU_RS09625 0.83 -2.0
2,126,611 - +3.0
2,126,707 + +3.0
2,126,707 + +1.1
2,126,737 - -1.1
2,126,737 - -1.3
2,126,737 - -0.5

Or see this region's nucleotide sequence