Experiment: Choline chloride 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS03955 and PFLU_RS03960 are separated by 48 nucleotides PFLU_RS03960 and PFLU_RS03965 are separated by 159 nucleotides
PFLU_RS03955: PFLU_RS03955 - O-antigen ligase family protein, at 892,746 to 894,146
_RS03955
PFLU_RS03960: PFLU_RS03960 - tRNA-Thr, at 894,195 to 894,267
_RS03960
PFLU_RS03965: PFLU_RS03965 - hypothetical protein, at 894,427 to 894,732
_RS03965
Position (kb) 894
895 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 893.235 kb on + strand, within PFLU_RS03955 at 893.235 kb on + strand, within PFLU_RS03955 at 893.235 kb on + strand, within PFLU_RS03955 at 893.235 kb on + strand, within PFLU_RS03955 at 893.236 kb on - strand, within PFLU_RS03955 at 893.236 kb on - strand, within PFLU_RS03955 at 893.484 kb on + strand, within PFLU_RS03955 at 893.484 kb on + strand, within PFLU_RS03955 at 893.484 kb on + strand, within PFLU_RS03955 at 893.484 kb on + strand, within PFLU_RS03955 at 893.484 kb on + strand, within PFLU_RS03955 at 893.485 kb on - strand, within PFLU_RS03955 at 893.485 kb on - strand, within PFLU_RS03955 at 893.514 kb on + strand, within PFLU_RS03955 at 893.515 kb on - strand, within PFLU_RS03955 at 893.515 kb on - strand, within PFLU_RS03955 at 893.515 kb on - strand, within PFLU_RS03955 at 893.779 kb on + strand, within PFLU_RS03955 at 893.780 kb on - strand, within PFLU_RS03955 at 893.990 kb on + strand, within PFLU_RS03955 at 893.990 kb on + strand, within PFLU_RS03955 at 893.990 kb on + strand, within PFLU_RS03955 at 893.990 kb on + strand, within PFLU_RS03955 at 893.990 kb on + strand, within PFLU_RS03955 at 893.990 kb on + strand, within PFLU_RS03955 at 893.990 kb on + strand, within PFLU_RS03955 at 893.990 kb on + strand, within PFLU_RS03955 at 893.990 kb on + strand, within PFLU_RS03955 at 893.990 kb on + strand, within PFLU_RS03955 at 893.990 kb on + strand, within PFLU_RS03955 at 893.991 kb on - strand, within PFLU_RS03955 at 893.991 kb on - strand, within PFLU_RS03955 at 893.991 kb on - strand, within PFLU_RS03955 at 893.991 kb on - strand, within PFLU_RS03955 at 893.991 kb on - strand, within PFLU_RS03955 at 893.991 kb on - strand, within PFLU_RS03955 at 893.991 kb on - strand, within PFLU_RS03955 at 893.991 kb on - strand, within PFLU_RS03955 at 893.991 kb on - strand, within PFLU_RS03955 at 893.991 kb on - strand, within PFLU_RS03955 at 893.991 kb on - strand, within PFLU_RS03955 at 893.991 kb on - strand, within PFLU_RS03955 at 893.991 kb on - strand, within PFLU_RS03955 at 893.992 kb on + strand, within PFLU_RS03955 at 893.992 kb on + strand, within PFLU_RS03955 at 893.992 kb on + strand, within PFLU_RS03955 at 893.993 kb on - strand, within PFLU_RS03955 at 894.052 kb on + strand at 894.052 kb on + strand at 894.052 kb on + strand at 894.052 kb on + strand at 894.052 kb on + strand at 894.053 kb on - strand at 894.053 kb on - strand at 894.053 kb on - strand at 894.053 kb on - strand at 894.053 kb on - strand at 894.053 kb on - strand at 894.053 kb on - strand at 894.053 kb on - strand at 894.053 kb on - strand at 894.053 kb on - strand at 894.053 kb on - strand at 894.081 kb on + strand at 894.081 kb on + strand at 894.081 kb on + strand at 894.082 kb on - strand at 894.102 kb on + strand at 894.102 kb on + strand at 894.102 kb on + strand at 894.102 kb on + strand at 894.102 kb on + strand at 894.102 kb on + strand at 894.102 kb on + strand at 894.102 kb on + strand at 894.102 kb on + strand at 894.102 kb on + strand at 894.102 kb on + strand at 894.102 kb on + strand at 894.102 kb on + strand at 894.102 kb on + strand at 894.103 kb on - strand at 894.103 kb on - strand at 894.103 kb on - strand at 894.103 kb on - strand at 894.103 kb on - strand at 894.103 kb on - strand at 894.180 kb on + strand at 894.180 kb on + strand at 894.180 kb on + strand at 894.181 kb on - strand at 894.181 kb on - strand at 894.181 kb on - strand at 894.181 kb on - strand at 894.181 kb on - strand at 894.181 kb on - strand at 894.181 kb on - strand at 894.186 kb on + strand at 894.187 kb on - strand at 894.202 kb on + strand at 894.202 kb on + strand at 894.203 kb on - strand, within PFLU_RS03960 at 894.203 kb on - strand, within PFLU_RS03960 at 894.203 kb on - strand, within PFLU_RS03960 at 894.203 kb on - strand, within PFLU_RS03960 at 894.231 kb on - strand, within PFLU_RS03960 at 894.369 kb on + strand at 894.369 kb on + strand at 894.370 kb on - strand at 894.370 kb on - strand at 894.370 kb on - strand at 894.370 kb on - strand at 894.415 kb on + strand at 894.415 kb on + strand at 894.415 kb on + strand at 894.415 kb on + strand at 894.415 kb on + strand at 894.415 kb on + strand at 894.416 kb on - strand at 894.416 kb on - strand at 894.416 kb on - strand at 894.481 kb on + strand, within PFLU_RS03965 at 894.481 kb on + strand, within PFLU_RS03965 at 894.481 kb on + strand, within PFLU_RS03965 at 894.481 kb on + strand, within PFLU_RS03965 at 894.481 kb on + strand, within PFLU_RS03965 at 894.481 kb on + strand, within PFLU_RS03965 at 894.482 kb on - strand, within PFLU_RS03965 at 894.482 kb on - strand, within PFLU_RS03965 at 894.482 kb on - strand, within PFLU_RS03965 at 894.504 kb on + strand, within PFLU_RS03965 at 894.505 kb on - strand, within PFLU_RS03965 at 894.505 kb on - strand, within PFLU_RS03965 at 894.505 kb on - strand, within PFLU_RS03965 at 894.505 kb on - strand, within PFLU_RS03965 at 894.505 kb on - strand, within PFLU_RS03965 at 894.589 kb on + strand, within PFLU_RS03965 at 894.616 kb on + strand, within PFLU_RS03965 at 894.616 kb on + strand, within PFLU_RS03965 at 894.616 kb on + strand, within PFLU_RS03965 at 894.616 kb on + strand, within PFLU_RS03965 at 894.616 kb on + strand, within PFLU_RS03965 at 894.616 kb on + strand, within PFLU_RS03965 at 894.616 kb on + strand, within PFLU_RS03965 at 894.616 kb on + strand, within PFLU_RS03965 at 894.617 kb on - strand, within PFLU_RS03965 at 894.617 kb on - strand, within PFLU_RS03965 at 894.617 kb on - strand, within PFLU_RS03965 at 894.617 kb on - strand, within PFLU_RS03965 at 894.617 kb on - strand, within PFLU_RS03965 at 894.617 kb on - strand, within PFLU_RS03965 at 894.663 kb on + strand, within PFLU_RS03965 at 894.663 kb on + strand, within PFLU_RS03965 at 894.663 kb on + strand, within PFLU_RS03965 at 894.663 kb on + strand, within PFLU_RS03965 at 894.664 kb on - strand, within PFLU_RS03965 at 894.664 kb on - strand, within PFLU_RS03965 at 894.664 kb on - strand, within PFLU_RS03965 at 894.664 kb on - strand, within PFLU_RS03965 at 894.664 kb on - strand, within PFLU_RS03965 at 894.664 kb on - strand, within PFLU_RS03965 at 894.664 kb on - strand, within PFLU_RS03965 at 894.664 kb on - strand, within PFLU_RS03965 at 894.664 kb on - strand, within PFLU_RS03965 at 894.664 kb on - strand, within PFLU_RS03965 at 894.664 kb on - strand, within PFLU_RS03965 at 894.704 kb on - strand at 894.704 kb on - strand at 894.722 kb on + strand at 894.722 kb on + strand at 894.722 kb on + strand at 894.723 kb on - strand at 894.723 kb on - strand at 894.731 kb on - strand at 894.743 kb on - strand at 894.743 kb on - strand at 894.743 kb on - strand at 894.899 kb on + strand at 894.899 kb on + strand at 894.899 kb on + strand at 894.899 kb on + strand at 894.899 kb on + strand at 894.899 kb on + strand at 894.900 kb on - strand at 894.900 kb on - strand at 894.900 kb on - strand at 894.900 kb on - strand at 894.900 kb on - strand at 894.900 kb on - strand at 894.900 kb on - strand at 894.900 kb on - strand at 894.900 kb on - strand at 894.900 kb on - strand at 894.900 kb on - strand at 894.900 kb on - strand at 894.900 kb on - strand at 894.900 kb on - strand at 894.900 kb on - strand at 894.923 kb on + strand at 894.951 kb on - strand at 894.963 kb on - strand at 895.023 kb on + strand at 895.023 kb on + strand at 895.023 kb on + strand at 895.024 kb on - strand at 895.024 kb on - strand at 895.024 kb on - strand at 895.024 kb on - strand at 895.059 kb on - strand at 895.116 kb on + strand at 895.116 kb on + strand at 895.117 kb on - strand at 895.117 kb on - strand at 895.117 kb on - strand at 895.119 kb on + strand at 895.119 kb on + strand at 895.119 kb on + strand at 895.119 kb on + strand at 895.119 kb on + strand at 895.119 kb on + strand at 895.119 kb on + strand at 895.119 kb on + strand at 895.120 kb on - strand at 895.120 kb on - strand at 895.120 kb on - strand at 895.120 kb on - strand at 895.120 kb on - strand at 895.120 kb on - strand at 895.120 kb on - strand at 895.120 kb on - strand at 895.120 kb on - strand at 895.120 kb on - strand at 895.120 kb on - strand at 895.120 kb on - strand at 895.120 kb on - strand at 895.120 kb on - strand at 895.183 kb on - strand at 895.183 kb on - strand at 895.183 kb on - strand at 895.183 kb on - strand at 895.183 kb on - strand at 895.183 kb on - strand at 895.183 kb on - strand at 895.183 kb on - strand at 895.183 kb on - strand at 895.183 kb on - strand at 895.183 kb on - strand at 895.183 kb on - strand at 895.183 kb on - strand at 895.183 kb on - strand at 895.183 kb on - strand at 895.183 kb on - strand at 895.230 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 10 mM (C) remove 893,235 + PFLU_RS03955 0.35 +0.4 893,235 + PFLU_RS03955 0.35 +0.4 893,235 + PFLU_RS03955 0.35 +0.4 893,235 + PFLU_RS03955 0.35 +1.7 893,236 - PFLU_RS03955 0.35 +0.2 893,236 - PFLU_RS03955 0.35 -2.2 893,484 + PFLU_RS03955 0.53 -1.8 893,484 + PFLU_RS03955 0.53 -1.8 893,484 + PFLU_RS03955 0.53 -3.0 893,484 + PFLU_RS03955 0.53 +0.7 893,484 + PFLU_RS03955 0.53 +0.3 893,485 - PFLU_RS03955 0.53 +0.3 893,485 - PFLU_RS03955 0.53 -1.3 893,514 + PFLU_RS03955 0.55 +0.4 893,515 - PFLU_RS03955 0.55 -1.1 893,515 - PFLU_RS03955 0.55 -1.8 893,515 - PFLU_RS03955 0.55 +1.3 893,779 + PFLU_RS03955 0.74 +0.3 893,780 - PFLU_RS03955 0.74 +1.1 893,990 + PFLU_RS03955 0.89 -1.4 893,990 + PFLU_RS03955 0.89 +1.2 893,990 + PFLU_RS03955 0.89 -2.1 893,990 + PFLU_RS03955 0.89 -1.1 893,990 + PFLU_RS03955 0.89 +1.5 893,990 + PFLU_RS03955 0.89 +2.1 893,990 + PFLU_RS03955 0.89 +2.5 893,990 + PFLU_RS03955 0.89 -1.4 893,990 + PFLU_RS03955 0.89 -2.1 893,990 + PFLU_RS03955 0.89 +3.1 893,990 + PFLU_RS03955 0.89 -1.5 893,991 - PFLU_RS03955 0.89 +1.8 893,991 - PFLU_RS03955 0.89 +0.7 893,991 - PFLU_RS03955 0.89 -1.1 893,991 - PFLU_RS03955 0.89 +0.7 893,991 - PFLU_RS03955 0.89 +0.5 893,991 - PFLU_RS03955 0.89 +0.7 893,991 - PFLU_RS03955 0.89 -1.2 893,991 - PFLU_RS03955 0.89 -0.1 893,991 - PFLU_RS03955 0.89 +0.7 893,991 - PFLU_RS03955 0.89 +1.3 893,991 - PFLU_RS03955 0.89 +0.0 893,991 - PFLU_RS03955 0.89 +0.8 893,991 - PFLU_RS03955 0.89 -0.4 893,992 + PFLU_RS03955 0.89 +0.1 893,992 + PFLU_RS03955 0.89 -2.7 893,992 + PFLU_RS03955 0.89 +0.7 893,993 - PFLU_RS03955 0.89 +1.2 894,052 + +0.3 894,052 + +3.6 894,052 + +1.0 894,052 + +0.1 894,052 + -3.1 894,053 - +1.7 894,053 - +3.4 894,053 - +0.4 894,053 - +1.1 894,053 - -1.0 894,053 - -0.9 894,053 - +0.6 894,053 - -0.9 894,053 - -0.4 894,053 - -0.4 894,053 - -0.3 894,081 + +1.0 894,081 + +0.9 894,081 + -1.5 894,082 - +0.3 894,102 + +1.7 894,102 + -2.1 894,102 + -0.1 894,102 + +1.0 894,102 + +0.0 894,102 + -1.5 894,102 + -0.1 894,102 + +0.2 894,102 + +0.3 894,102 + -1.7 894,102 + -0.4 894,102 + +0.9 894,102 + -1.7 894,102 + -2.8 894,103 - +0.9 894,103 - +3.7 894,103 - -2.2 894,103 - +0.3 894,103 - -1.4 894,103 - -2.1 894,180 + -3.1 894,180 + -0.0 894,180 + +2.5 894,181 - -0.0 894,181 - -2.4 894,181 - -1.4 894,181 - -3.0 894,181 - -0.9 894,181 - +3.5 894,181 - -1.5 894,186 + +0.3 894,187 - -1.9 894,202 + +0.3 894,202 + +0.9 894,203 - PFLU_RS03960 0.11 +2.0 894,203 - PFLU_RS03960 0.11 -2.1 894,203 - PFLU_RS03960 0.11 -0.7 894,203 - PFLU_RS03960 0.11 +1.9 894,231 - PFLU_RS03960 0.49 +1.1 894,369 + -1.1 894,369 + +0.3 894,370 - +0.2 894,370 - +0.6 894,370 - -2.7 894,370 - -2.4 894,415 + +1.4 894,415 + -0.1 894,415 + -1.1 894,415 + +0.8 894,415 + +0.6 894,415 + -2.2 894,416 - -1.7 894,416 - +0.6 894,416 - +0.5 894,481 + PFLU_RS03965 0.18 +1.4 894,481 + PFLU_RS03965 0.18 +0.8 894,481 + PFLU_RS03965 0.18 -2.1 894,481 + PFLU_RS03965 0.18 -1.2 894,481 + PFLU_RS03965 0.18 -1.4 894,481 + PFLU_RS03965 0.18 +1.1 894,482 - PFLU_RS03965 0.18 -2.6 894,482 - PFLU_RS03965 0.18 -0.5 894,482 - PFLU_RS03965 0.18 -1.4 894,504 + PFLU_RS03965 0.25 +1.9 894,505 - PFLU_RS03965 0.25 -1.9 894,505 - PFLU_RS03965 0.25 +0.2 894,505 - PFLU_RS03965 0.25 +2.2 894,505 - PFLU_RS03965 0.25 -1.1 894,505 - PFLU_RS03965 0.25 -1.9 894,589 + PFLU_RS03965 0.53 +0.3 894,616 + PFLU_RS03965 0.62 -0.4 894,616 + PFLU_RS03965 0.62 +0.4 894,616 + PFLU_RS03965 0.62 -0.1 894,616 + PFLU_RS03965 0.62 -1.2 894,616 + PFLU_RS03965 0.62 -0.2 894,616 + PFLU_RS03965 0.62 -0.1 894,616 + PFLU_RS03965 0.62 +0.2 894,616 + PFLU_RS03965 0.62 -1.1 894,617 - PFLU_RS03965 0.62 -1.4 894,617 - PFLU_RS03965 0.62 -0.1 894,617 - PFLU_RS03965 0.62 -2.1 894,617 - PFLU_RS03965 0.62 +1.2 894,617 - PFLU_RS03965 0.62 -1.4 894,617 - PFLU_RS03965 0.62 -0.1 894,663 + PFLU_RS03965 0.77 -1.2 894,663 + PFLU_RS03965 0.77 -0.5 894,663 + PFLU_RS03965 0.77 +1.1 894,663 + PFLU_RS03965 0.77 -2.1 894,664 - PFLU_RS03965 0.77 -1.8 894,664 - PFLU_RS03965 0.77 +0.3 894,664 - PFLU_RS03965 0.77 +0.5 894,664 - PFLU_RS03965 0.77 +1.0 894,664 - PFLU_RS03965 0.77 -0.4 894,664 - PFLU_RS03965 0.77 -2.8 894,664 - PFLU_RS03965 0.77 -1.5 894,664 - PFLU_RS03965 0.77 -0.2 894,664 - PFLU_RS03965 0.77 +0.6 894,664 - PFLU_RS03965 0.77 -1.7 894,664 - PFLU_RS03965 0.77 -1.2 894,704 - -1.4 894,704 - +1.7 894,722 + +2.9 894,722 + -1.6 894,722 + -1.1 894,723 - -1.8 894,723 - -0.4 894,731 - +1.5 894,743 - +1.5 894,743 - +1.2 894,743 - +1.4 894,899 + -0.9 894,899 + +2.4 894,899 + -0.9 894,899 + -0.5 894,899 + -0.9 894,899 + +1.2 894,900 - +1.9 894,900 - +0.1 894,900 - +0.6 894,900 - -0.1 894,900 - +0.7 894,900 - +2.1 894,900 - -0.1 894,900 - -0.4 894,900 - +1.5 894,900 - -2.4 894,900 - -1.8 894,900 - -2.9 894,900 - +0.2 894,900 - +1.4 894,900 - +1.9 894,923 + -1.9 894,951 - +1.1 894,963 - +0.0 895,023 + +0.9 895,023 + -0.3 895,023 + +1.7 895,024 - -0.1 895,024 - +1.9 895,024 - -1.1 895,024 - +1.3 895,059 - -0.2 895,116 + +0.3 895,116 + -1.5 895,117 - +0.8 895,117 - -1.4 895,117 - +1.5 895,119 + -2.2 895,119 + +1.0 895,119 + -0.3 895,119 + -2.2 895,119 + -1.2 895,119 + +1.9 895,119 + -2.8 895,119 + -0.2 895,120 - -2.2 895,120 - -0.8 895,120 - +1.0 895,120 - +1.2 895,120 - +0.9 895,120 - +0.4 895,120 - -0.1 895,120 - +1.9 895,120 - -0.9 895,120 - +0.7 895,120 - +1.5 895,120 - +0.5 895,120 - +1.5 895,120 - +1.6 895,183 - +1.3 895,183 - -2.2 895,183 - -0.9 895,183 - +0.3 895,183 - -0.4 895,183 - -1.8 895,183 - -0.4 895,183 - -0.8 895,183 - -1.1 895,183 - +1.3 895,183 - -1.0 895,183 - -1.2 895,183 - +0.9 895,183 - -1.1 895,183 - +0.3 895,183 - +1.9 895,230 + +1.9
Or see this region's nucleotide sequence