Experiment: Choline chloride 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS02000 and PFLU_RS02005 are separated by 217 nucleotides PFLU_RS02005 and PFLU_RS02010 overlap by 4 nucleotides PFLU_RS02010 and PFLU_RS02015 overlap by 4 nucleotides
PFLU_RS02000: PFLU_RS02000 - penicillin-binding protein 1A, at 448,741 to 451,203
_RS02000
PFLU_RS02005: PFLU_RS02005 - type IV pilus assembly protein PilM, at 451,421 to 452,074
_RS02005
PFLU_RS02010: PFLU_RS02010 - fimbriae biosynthesis protein, at 452,071 to 452,625
_RS02010
PFLU_RS02015: PFLU_RS02015 - type 4a pilus biogenesis protein PilO, at 452,622 to 453,587
_RS02015
Position (kb) 452
453 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 451.077 kb on + strand at 451.077 kb on + strand at 451.078 kb on - strand at 451.078 kb on - strand at 451.078 kb on - strand at 451.078 kb on - strand at 451.093 kb on + strand at 451.093 kb on + strand at 451.094 kb on - strand at 451.113 kb on + strand at 451.113 kb on + strand at 451.113 kb on + strand at 451.113 kb on + strand at 451.113 kb on + strand at 451.113 kb on + strand at 451.114 kb on - strand at 451.114 kb on - strand at 451.114 kb on - strand at 451.114 kb on - strand at 451.114 kb on - strand at 451.114 kb on - strand at 451.118 kb on + strand at 451.124 kb on + strand at 451.124 kb on + strand at 451.124 kb on + strand at 451.125 kb on - strand at 451.175 kb on + strand at 451.235 kb on + strand at 451.284 kb on + strand at 451.284 kb on + strand at 451.285 kb on - strand at 451.310 kb on + strand at 451.311 kb on - strand at 451.393 kb on + strand at 451.394 kb on - strand at 451.488 kb on + strand, within PFLU_RS02005 at 451.489 kb on - strand, within PFLU_RS02005 at 451.489 kb on - strand, within PFLU_RS02005 at 451.489 kb on - strand, within PFLU_RS02005 at 451.523 kb on + strand, within PFLU_RS02005 at 451.523 kb on + strand, within PFLU_RS02005 at 451.523 kb on + strand, within PFLU_RS02005 at 451.524 kb on - strand, within PFLU_RS02005 at 451.524 kb on - strand, within PFLU_RS02005 at 451.538 kb on + strand, within PFLU_RS02005 at 451.538 kb on + strand, within PFLU_RS02005 at 451.538 kb on + strand, within PFLU_RS02005 at 451.539 kb on - strand, within PFLU_RS02005 at 451.539 kb on - strand, within PFLU_RS02005 at 451.539 kb on - strand, within PFLU_RS02005 at 451.763 kb on + strand, within PFLU_RS02005 at 451.763 kb on + strand, within PFLU_RS02005 at 451.763 kb on + strand, within PFLU_RS02005 at 451.763 kb on + strand, within PFLU_RS02005 at 451.763 kb on + strand, within PFLU_RS02005 at 451.763 kb on + strand, within PFLU_RS02005 at 451.763 kb on + strand, within PFLU_RS02005 at 451.763 kb on + strand, within PFLU_RS02005 at 451.763 kb on + strand, within PFLU_RS02005 at 451.764 kb on - strand, within PFLU_RS02005 at 451.764 kb on - strand, within PFLU_RS02005 at 451.764 kb on - strand, within PFLU_RS02005 at 451.764 kb on - strand, within PFLU_RS02005 at 451.764 kb on - strand, within PFLU_RS02005 at 451.824 kb on + strand, within PFLU_RS02005 at 451.824 kb on + strand, within PFLU_RS02005 at 451.825 kb on - strand, within PFLU_RS02005 at 451.825 kb on - strand, within PFLU_RS02005 at 451.825 kb on - strand, within PFLU_RS02005 at 451.845 kb on + strand, within PFLU_RS02005 at 451.845 kb on + strand, within PFLU_RS02005 at 452.090 kb on + strand at 452.090 kb on + strand at 452.090 kb on + strand at 452.090 kb on + strand at 452.090 kb on + strand at 452.090 kb on + strand at 452.090 kb on + strand at 452.090 kb on + strand at 452.090 kb on + strand at 452.090 kb on + strand at 452.090 kb on + strand at 452.090 kb on + strand at 452.090 kb on + strand at 452.090 kb on + strand at 452.091 kb on - strand at 452.091 kb on - strand at 452.091 kb on - strand at 452.091 kb on - strand at 452.091 kb on - strand at 452.091 kb on - strand at 452.091 kb on - strand at 452.091 kb on - strand at 452.091 kb on - strand at 452.091 kb on - strand at 452.091 kb on - strand at 452.091 kb on - strand at 452.091 kb on - strand at 452.091 kb on - strand at 452.091 kb on - strand at 452.091 kb on - strand at 452.091 kb on - strand at 452.131 kb on + strand, within PFLU_RS02010 at 452.131 kb on + strand, within PFLU_RS02010 at 452.131 kb on + strand, within PFLU_RS02010 at 452.131 kb on + strand, within PFLU_RS02010 at 452.131 kb on + strand, within PFLU_RS02010 at 452.131 kb on + strand, within PFLU_RS02010 at 452.131 kb on + strand, within PFLU_RS02010 at 452.131 kb on + strand, within PFLU_RS02010 at 452.132 kb on - strand, within PFLU_RS02010 at 452.132 kb on - strand, within PFLU_RS02010 at 452.132 kb on - strand, within PFLU_RS02010 at 452.132 kb on - strand, within PFLU_RS02010 at 452.132 kb on - strand, within PFLU_RS02010 at 452.132 kb on - strand, within PFLU_RS02010 at 452.132 kb on - strand, within PFLU_RS02010 at 452.181 kb on - strand, within PFLU_RS02010 at 452.451 kb on + strand, within PFLU_RS02010 at 452.451 kb on + strand, within PFLU_RS02010 at 452.452 kb on - strand, within PFLU_RS02010 at 452.452 kb on - strand, within PFLU_RS02010 at 452.452 kb on - strand, within PFLU_RS02010 at 452.680 kb on + strand at 452.680 kb on + strand at 452.680 kb on + strand at 452.680 kb on + strand at 452.745 kb on + strand, within PFLU_RS02015 at 452.745 kb on + strand, within PFLU_RS02015 at 452.745 kb on + strand, within PFLU_RS02015 at 452.745 kb on + strand, within PFLU_RS02015 at 452.745 kb on + strand, within PFLU_RS02015 at 452.745 kb on + strand, within PFLU_RS02015 at 452.746 kb on - strand, within PFLU_RS02015 at 452.746 kb on - strand, within PFLU_RS02015 at 452.746 kb on - strand, within PFLU_RS02015 at 452.746 kb on - strand, within PFLU_RS02015 at 453.019 kb on - strand, within PFLU_RS02015 at 453.019 kb on - strand, within PFLU_RS02015 at 453.019 kb on - strand, within PFLU_RS02015 at 453.019 kb on - strand, within PFLU_RS02015 at 453.019 kb on - strand, within PFLU_RS02015 at 453.019 kb on - strand, within PFLU_RS02015 at 453.019 kb on - strand, within PFLU_RS02015 at 453.057 kb on + strand, within PFLU_RS02015 at 453.057 kb on + strand, within PFLU_RS02015 at 453.057 kb on + strand, within PFLU_RS02015 at 453.058 kb on - strand, within PFLU_RS02015 at 453.058 kb on - strand, within PFLU_RS02015 at 453.058 kb on - strand, within PFLU_RS02015 at 453.058 kb on - strand, within PFLU_RS02015 at 453.058 kb on - strand, within PFLU_RS02015 at 453.058 kb on - strand, within PFLU_RS02015 at 453.092 kb on + strand, within PFLU_RS02015 at 453.234 kb on + strand, within PFLU_RS02015 at 453.234 kb on + strand, within PFLU_RS02015 at 453.239 kb on + strand, within PFLU_RS02015 at 453.456 kb on + strand, within PFLU_RS02015 at 453.456 kb on + strand, within PFLU_RS02015 at 453.456 kb on + strand, within PFLU_RS02015 at 453.456 kb on + strand, within PFLU_RS02015 at 453.456 kb on + strand, within PFLU_RS02015 at 453.456 kb on + strand, within PFLU_RS02015 at 453.501 kb on + strand at 453.501 kb on + strand at 453.501 kb on + strand at 453.501 kb on + strand at 453.501 kb on + strand at 453.501 kb on + strand at 453.501 kb on + strand at 453.501 kb on + strand at 453.502 kb on - strand at 453.502 kb on - strand at 453.502 kb on - strand at 453.502 kb on - strand at 453.502 kb on - strand at 453.502 kb on - strand at 453.502 kb on - strand at 453.502 kb on - strand at 453.502 kb on - strand at 453.502 kb on - strand at 453.502 kb on - strand at 453.502 kb on - strand at 453.502 kb on - strand at 453.502 kb on - strand at 453.502 kb on - strand at 453.502 kb on - strand at 453.502 kb on - strand at 453.503 kb on + strand at 453.503 kb on + strand at 453.503 kb on + strand at 453.503 kb on + strand at 453.503 kb on + strand at 453.503 kb on + strand at 453.503 kb on + strand at 453.503 kb on + strand at 453.503 kb on + strand at 453.503 kb on + strand at 453.503 kb on + strand at 453.503 kb on + strand at 453.503 kb on + strand at 453.503 kb on + strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.504 kb on - strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.505 kb on + strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.506 kb on - strand at 453.572 kb on - strand at 453.572 kb on - strand at 453.585 kb on + strand at 453.585 kb on + strand at 453.586 kb on - strand at 453.586 kb on - strand at 453.586 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 10 mM (C) remove 451,077 + -2.4 451,077 + +2.0 451,078 - -1.6 451,078 - +0.2 451,078 - +0.9 451,078 - +2.2 451,093 + +3.0 451,093 + +0.0 451,094 - +0.6 451,113 + -1.0 451,113 + -0.4 451,113 + -2.2 451,113 + +3.3 451,113 + -2.0 451,113 + +1.8 451,114 - +4.6 451,114 - +0.8 451,114 - +0.0 451,114 - -1.2 451,114 - -0.1 451,114 - +2.4 451,118 + -2.0 451,124 + -0.6 451,124 + -2.0 451,124 + +2.2 451,125 - +2.3 451,175 + +1.7 451,235 + -1.9 451,284 + -2.3 451,284 + +2.0 451,285 - +2.4 451,310 + +0.8 451,311 - -2.2 451,393 + +1.9 451,394 - -1.0 451,488 + PFLU_RS02005 0.10 +0.3 451,489 - PFLU_RS02005 0.10 -1.9 451,489 - PFLU_RS02005 0.10 +0.8 451,489 - PFLU_RS02005 0.10 -0.4 451,523 + PFLU_RS02005 0.16 -3.2 451,523 + PFLU_RS02005 0.16 +0.6 451,523 + PFLU_RS02005 0.16 -1.2 451,524 - PFLU_RS02005 0.16 -3.0 451,524 - PFLU_RS02005 0.16 +2.9 451,538 + PFLU_RS02005 0.18 +1.6 451,538 + PFLU_RS02005 0.18 +1.0 451,538 + PFLU_RS02005 0.18 +1.1 451,539 - PFLU_RS02005 0.18 +1.8 451,539 - PFLU_RS02005 0.18 -2.3 451,539 - PFLU_RS02005 0.18 -1.2 451,763 + PFLU_RS02005 0.52 -1.3 451,763 + PFLU_RS02005 0.52 -2.2 451,763 + PFLU_RS02005 0.52 +0.8 451,763 + PFLU_RS02005 0.52 +0.1 451,763 + PFLU_RS02005 0.52 -0.6 451,763 + PFLU_RS02005 0.52 -1.8 451,763 + PFLU_RS02005 0.52 -3.0 451,763 + PFLU_RS02005 0.52 +1.5 451,763 + PFLU_RS02005 0.52 -2.4 451,764 - PFLU_RS02005 0.52 +3.1 451,764 - PFLU_RS02005 0.52 -0.3 451,764 - PFLU_RS02005 0.52 +1.8 451,764 - PFLU_RS02005 0.52 -0.2 451,764 - PFLU_RS02005 0.52 -1.2 451,824 + PFLU_RS02005 0.62 -3.0 451,824 + PFLU_RS02005 0.62 +0.4 451,825 - PFLU_RS02005 0.62 -0.3 451,825 - PFLU_RS02005 0.62 -0.6 451,825 - PFLU_RS02005 0.62 -1.3 451,845 + PFLU_RS02005 0.65 -3.4 451,845 + PFLU_RS02005 0.65 -0.9 452,090 + -1.3 452,090 + -0.7 452,090 + +0.1 452,090 + -1.4 452,090 + +2.6 452,090 + +0.4 452,090 + -0.6 452,090 + +0.9 452,090 + +0.4 452,090 + -0.0 452,090 + -1.0 452,090 + -0.6 452,090 + +1.2 452,090 + -0.6 452,091 - -1.2 452,091 - -1.7 452,091 - +0.4 452,091 - -0.5 452,091 - +3.3 452,091 - -0.8 452,091 - +0.7 452,091 - -0.9 452,091 - -0.3 452,091 - -2.8 452,091 - -1.9 452,091 - -0.8 452,091 - +0.0 452,091 - -2.1 452,091 - +0.9 452,091 - +0.2 452,091 - -0.8 452,131 + PFLU_RS02010 0.11 +0.8 452,131 + PFLU_RS02010 0.11 +1.0 452,131 + PFLU_RS02010 0.11 +0.4 452,131 + PFLU_RS02010 0.11 -1.0 452,131 + PFLU_RS02010 0.11 +2.9 452,131 + PFLU_RS02010 0.11 +0.2 452,131 + PFLU_RS02010 0.11 +1.2 452,131 + PFLU_RS02010 0.11 +0.9 452,132 - PFLU_RS02010 0.11 +2.0 452,132 - PFLU_RS02010 0.11 +0.2 452,132 - PFLU_RS02010 0.11 +1.9 452,132 - PFLU_RS02010 0.11 -2.2 452,132 - PFLU_RS02010 0.11 +3.8 452,132 - PFLU_RS02010 0.11 -2.1 452,132 - PFLU_RS02010 0.11 -1.6 452,181 - PFLU_RS02010 0.20 +0.0 452,451 + PFLU_RS02010 0.68 -2.3 452,451 + PFLU_RS02010 0.68 +0.7 452,452 - PFLU_RS02010 0.69 +1.1 452,452 - PFLU_RS02010 0.69 +1.3 452,452 - PFLU_RS02010 0.69 +0.7 452,680 + +0.7 452,680 + -0.8 452,680 + -3.1 452,680 + -2.4 452,745 + PFLU_RS02015 0.13 -1.3 452,745 + PFLU_RS02015 0.13 -1.4 452,745 + PFLU_RS02015 0.13 -2.2 452,745 + PFLU_RS02015 0.13 -0.6 452,745 + PFLU_RS02015 0.13 -1.4 452,745 + PFLU_RS02015 0.13 +3.6 452,746 - PFLU_RS02015 0.13 +2.0 452,746 - PFLU_RS02015 0.13 -0.3 452,746 - PFLU_RS02015 0.13 -0.6 452,746 - PFLU_RS02015 0.13 +2.0 453,019 - PFLU_RS02015 0.41 +0.0 453,019 - PFLU_RS02015 0.41 -0.6 453,019 - PFLU_RS02015 0.41 -2.3 453,019 - PFLU_RS02015 0.41 -0.3 453,019 - PFLU_RS02015 0.41 +1.7 453,019 - PFLU_RS02015 0.41 +0.7 453,019 - PFLU_RS02015 0.41 -0.3 453,057 + PFLU_RS02015 0.45 -1.4 453,057 + PFLU_RS02015 0.45 -0.2 453,057 + PFLU_RS02015 0.45 +1.7 453,058 - PFLU_RS02015 0.45 +0.7 453,058 - PFLU_RS02015 0.45 -1.6 453,058 - PFLU_RS02015 0.45 +0.4 453,058 - PFLU_RS02015 0.45 -0.8 453,058 - PFLU_RS02015 0.45 +0.4 453,058 - PFLU_RS02015 0.45 +2.0 453,092 + PFLU_RS02015 0.49 -2.0 453,234 + PFLU_RS02015 0.63 -3.1 453,234 + PFLU_RS02015 0.63 -1.3 453,239 + PFLU_RS02015 0.64 +0.6 453,456 + PFLU_RS02015 0.86 +5.0 453,456 + PFLU_RS02015 0.86 -1.8 453,456 + PFLU_RS02015 0.86 -0.6 453,456 + PFLU_RS02015 0.86 +0.3 453,456 + PFLU_RS02015 0.86 -0.8 453,456 + PFLU_RS02015 0.86 -1.9 453,501 + +1.1 453,501 + -2.5 453,501 + +2.0 453,501 + +0.8 453,501 + +1.5 453,501 + -1.0 453,501 + +1.4 453,501 + -0.3 453,502 - -0.1 453,502 - -2.0 453,502 - +1.2 453,502 - -1.3 453,502 - -1.9 453,502 - -2.2 453,502 - +0.1 453,502 - -2.5 453,502 - -2.2 453,502 - -0.4 453,502 - -1.0 453,502 - +1.9 453,502 - -0.6 453,502 - -2.8 453,502 - -0.2 453,502 - -0.8 453,502 - +0.2 453,503 + +2.0 453,503 + +0.6 453,503 + -0.2 453,503 + -1.4 453,503 + +0.0 453,503 + +0.8 453,503 + -0.9 453,503 + +1.6 453,503 + -1.4 453,503 + +1.4 453,503 + +0.5 453,503 + +0.0 453,503 + +1.1 453,503 + -1.4 453,504 - -0.6 453,504 - +1.0 453,504 - +1.5 453,504 - +1.9 453,504 - -1.9 453,504 - -1.8 453,504 - +2.6 453,504 - -2.3 453,504 - -2.6 453,504 - -2.4 453,504 - -0.8 453,504 - +0.0 453,504 - +1.5 453,504 - -2.0 453,504 - +0.4 453,504 - +0.2 453,504 - +0.7 453,504 - +2.0 453,504 - -1.4 453,504 - +0.0 453,504 - -0.8 453,504 - +1.0 453,504 - +1.1 453,504 - +2.7 453,504 - -1.2 453,504 - +2.9 453,504 - -1.6 453,504 - +0.4 453,504 - +1.1 453,504 - -1.8 453,504 - -3.0 453,504 - +0.5 453,504 - -1.7 453,504 - -2.4 453,505 + +1.3 453,505 + +0.0 453,505 + -1.8 453,505 + +0.4 453,505 + +0.6 453,505 + +1.7 453,505 + -1.3 453,505 + -1.6 453,505 + -1.6 453,505 + +0.3 453,505 + -1.8 453,505 + +1.2 453,505 + -1.6 453,505 + +1.0 453,505 + -1.7 453,505 + -0.3 453,505 + +0.0 453,505 + +0.5 453,505 + -2.1 453,505 + +0.2 453,505 + +1.6 453,506 - +0.1 453,506 - -0.6 453,506 - +0.7 453,506 - +1.9 453,506 - +0.6 453,506 - +1.3 453,506 - +0.1 453,506 - +1.0 453,506 - +0.6 453,506 - -2.3 453,506 - -0.7 453,506 - +0.8 453,506 - -2.3 453,506 - +1.4 453,506 - -2.0 453,506 - +0.0 453,506 - +0.1 453,506 - +0.0 453,506 - +1.5 453,506 - -1.8 453,506 - +0.5 453,506 - +0.9 453,506 - -0.5 453,506 - +0.0 453,506 - +1.2 453,506 - -2.7 453,506 - -1.3 453,506 - -0.7 453,506 - +2.4 453,506 - +0.6 453,572 - +0.1 453,572 - -2.3 453,585 + -1.4 453,585 + +0.4 453,586 - -1.3 453,586 - +0.5 453,586 - +0.4
Or see this region's nucleotide sequence