Experiment: SJC03_cps7_0.1MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0103 and BT0104 overlap by 4 nucleotides BT0104 and BT0105 overlap by 4 nucleotides BT0105 and BT0106 are separated by 105 nucleotides
BT0103: BT0103 - conserved hypothetical protein (NCBI ptt file), at 95,169 to 96,704
BT0103
BT0104: BT0104 - hypothetical protein (NCBI ptt file), at 96,701 to 96,889
BT0104
BT0105: BT0105 - DNA topoisomerase I (Omega-protein) (NCBI ptt file), at 96,886 to 98,967
BT0105
BT0106: BT0106 - conserved hypothetical protein (NCBI ptt file), at 99,073 to 99,519
BT0106
Position (kb) 96
97
98
99 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 96.140 kb on - strand, within BT0103 at 96.332 kb on - strand, within BT0103 at 96.332 kb on - strand, within BT0103 at 96.337 kb on + strand, within BT0103 at 96.417 kb on + strand, within BT0103 at 96.418 kb on - strand, within BT0103 at 96.426 kb on + strand, within BT0103 at 96.429 kb on - strand, within BT0103 at 96.830 kb on + strand, within BT0104 at 96.883 kb on - strand at 97.075 kb on - strand at 97.161 kb on - strand, within BT0105 at 97.161 kb on - strand, within BT0105 at 97.199 kb on + strand, within BT0105 at 97.406 kb on + strand, within BT0105 at 97.422 kb on + strand, within BT0105 at 97.423 kb on - strand, within BT0105 at 97.499 kb on + strand, within BT0105 at 97.500 kb on - strand, within BT0105 at 97.500 kb on - strand, within BT0105 at 97.543 kb on + strand, within BT0105 at 97.817 kb on + strand, within BT0105 at 97.817 kb on + strand, within BT0105 at 97.848 kb on + strand, within BT0105 at 97.903 kb on + strand, within BT0105 at 97.904 kb on - strand, within BT0105 at 98.276 kb on + strand, within BT0105 at 98.349 kb on + strand, within BT0105 at 98.452 kb on + strand, within BT0105 at 98.453 kb on - strand, within BT0105 at 98.499 kb on + strand, within BT0105 at 98.565 kb on + strand, within BT0105 at 98.630 kb on - strand, within BT0105 at 98.852 kb on + strand at 98.925 kb on + strand at 99.011 kb on - strand at 99.084 kb on + strand at 99.084 kb on + strand at 99.333 kb on + strand, within BT0106 at 99.374 kb on + strand, within BT0106 at 99.399 kb on + strand, within BT0106 at 99.407 kb on + strand, within BT0106 at 99.443 kb on + strand, within BT0106 at 99.482 kb on + strand at 99.482 kb on + strand at 99.574 kb on - strand at 99.574 kb on - strand at 99.584 kb on - strand at 99.616 kb on + strand at 99.622 kb on + strand at 99.638 kb on - strand at 99.666 kb on - strand at 99.723 kb on - strand at 99.731 kb on - strand at 99.731 kb on - strand at 99.808 kb on - strand at 99.812 kb on + strand at 99.813 kb on - strand at 99.817 kb on - strand at 99.841 kb on + strand at 99.841 kb on + strand at 99.842 kb on - strand at 99.842 kb on - strand at 99.857 kb on + strand at 99.877 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SJC03_cps7_0.1MOI remove 96,140 - BT0103 0.63 +1.3 96,332 - BT0103 0.76 -0.5 96,332 - BT0103 0.76 -1.8 96,337 + BT0103 0.76 -0.6 96,417 + BT0103 0.81 -0.5 96,418 - BT0103 0.81 -1.3 96,426 + BT0103 0.82 +1.3 96,429 - BT0103 0.82 -0.3 96,830 + BT0104 0.68 -3.3 96,883 - -0.0 97,075 - -0.6 97,161 - BT0105 0.13 -0.4 97,161 - BT0105 0.13 +1.0 97,199 + BT0105 0.15 -1.9 97,406 + BT0105 0.25 +0.1 97,422 + BT0105 0.26 -0.8 97,423 - BT0105 0.26 +3.1 97,499 + BT0105 0.29 +0.9 97,500 - BT0105 0.29 -0.8 97,500 - BT0105 0.29 -1.3 97,543 + BT0105 0.32 -0.9 97,817 + BT0105 0.45 -0.0 97,817 + BT0105 0.45 -2.1 97,848 + BT0105 0.46 -0.3 97,903 + BT0105 0.49 +0.8 97,904 - BT0105 0.49 -1.6 98,276 + BT0105 0.67 -0.6 98,349 + BT0105 0.70 -0.7 98,452 + BT0105 0.75 -1.6 98,453 - BT0105 0.75 -0.9 98,499 + BT0105 0.77 +0.9 98,565 + BT0105 0.81 +0.5 98,630 - BT0105 0.84 +1.0 98,852 + +0.3 98,925 + -1.0 99,011 - -1.5 99,084 + -1.3 99,084 + +0.1 99,333 + BT0106 0.58 -0.9 99,374 + BT0106 0.67 -1.1 99,399 + BT0106 0.73 -0.3 99,407 + BT0106 0.75 -1.7 99,443 + BT0106 0.83 -1.7 99,482 + +0.7 99,482 + -0.9 99,574 - -1.0 99,574 - -1.9 99,584 - +0.7 99,616 + -1.4 99,622 + -0.9 99,638 - -1.9 99,666 - -1.7 99,723 - -0.3 99,731 - +0.3 99,731 - -0.7 99,808 - +0.7 99,812 + -0.5 99,813 - -0.3 99,817 - -0.3 99,841 + -0.3 99,841 + +0.7 99,842 - -2.1 99,842 - -1.9 99,857 + -0.7 99,877 - -1.9
Or see this region's nucleotide sequence