Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS05350

Experiment: 4-hydroxyphenylacetic 2 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS05340 and PFLU_RS05345 are separated by 13 nucleotidesPFLU_RS05345 and PFLU_RS05350 are separated by 125 nucleotidesPFLU_RS05350 and PFLU_RS05355 overlap by 4 nucleotides PFLU_RS05340: PFLU_RS05340 - SDR family NAD(P)-dependent oxidoreductase, at 1,190,254 to 1,191,003 _RS05340 PFLU_RS05345: PFLU_RS05345 - hypothetical protein, at 1,191,017 to 1,191,310 _RS05345 PFLU_RS05350: PFLU_RS05350 - GTP-binding protein, at 1,191,436 to 1,192,410 _RS05350 PFLU_RS05355: PFLU_RS05355 - extracellular solute-binding protein, at 1,192,407 to 1,193,582 _RS05355 Position (kb) 1191 1192 1193Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1190.716 kb on + strand, within PFLU_RS05340at 1190.749 kb on - strand, within PFLU_RS05340at 1190.749 kb on - strand, within PFLU_RS05340at 1191.012 kb on + strandat 1191.012 kb on + strandat 1191.012 kb on + strandat 1191.012 kb on + strandat 1191.012 kb on + strandat 1191.013 kb on - strandat 1191.013 kb on - strandat 1191.013 kb on - strandat 1191.013 kb on - strandat 1191.176 kb on + strand, within PFLU_RS05345at 1191.176 kb on + strand, within PFLU_RS05345at 1191.177 kb on - strand, within PFLU_RS05345at 1191.177 kb on - strand, within PFLU_RS05345at 1191.200 kb on - strand, within PFLU_RS05345at 1191.435 kb on + strandat 1191.435 kb on + strandat 1191.435 kb on + strandat 1191.435 kb on + strandat 1191.436 kb on - strandat 1191.436 kb on - strandat 1191.436 kb on - strandat 1191.436 kb on - strandat 1191.436 kb on - strandat 1191.450 kb on + strandat 1191.450 kb on + strandat 1191.451 kb on - strandat 1191.451 kb on - strandat 1191.451 kb on - strandat 1191.451 kb on - strandat 1191.605 kb on + strand, within PFLU_RS05350at 1191.605 kb on + strand, within PFLU_RS05350at 1191.605 kb on + strand, within PFLU_RS05350at 1191.605 kb on + strand, within PFLU_RS05350at 1191.605 kb on + strand, within PFLU_RS05350at 1191.605 kb on + strand, within PFLU_RS05350at 1191.605 kb on + strand, within PFLU_RS05350at 1191.605 kb on + strand, within PFLU_RS05350at 1191.605 kb on + strand, within PFLU_RS05350at 1191.605 kb on + strand, within PFLU_RS05350at 1191.605 kb on + strand, within PFLU_RS05350at 1191.606 kb on - strand, within PFLU_RS05350at 1191.606 kb on - strand, within PFLU_RS05350at 1191.606 kb on - strand, within PFLU_RS05350at 1191.606 kb on - strand, within PFLU_RS05350at 1191.606 kb on - strand, within PFLU_RS05350at 1191.606 kb on - strand, within PFLU_RS05350at 1191.606 kb on - strand, within PFLU_RS05350at 1191.606 kb on - strand, within PFLU_RS05350at 1191.606 kb on - strand, within PFLU_RS05350at 1191.606 kb on - strand, within PFLU_RS05350at 1191.606 kb on - strand, within PFLU_RS05350at 1191.899 kb on + strand, within PFLU_RS05350at 1191.899 kb on + strand, within PFLU_RS05350at 1191.899 kb on + strand, within PFLU_RS05350at 1191.900 kb on - strand, within PFLU_RS05350at 1191.900 kb on - strand, within PFLU_RS05350at 1191.973 kb on - strand, within PFLU_RS05350at 1191.973 kb on - strand, within PFLU_RS05350at 1191.987 kb on + strand, within PFLU_RS05350at 1192.095 kb on + strand, within PFLU_RS05350at 1192.096 kb on - strand, within PFLU_RS05350at 1192.181 kb on + strand, within PFLU_RS05350at 1192.181 kb on + strand, within PFLU_RS05350at 1192.181 kb on + strand, within PFLU_RS05350at 1192.181 kb on + strand, within PFLU_RS05350at 1192.182 kb on - strand, within PFLU_RS05350at 1192.182 kb on - strand, within PFLU_RS05350at 1192.182 kb on - strand, within PFLU_RS05350at 1192.182 kb on - strand, within PFLU_RS05350at 1192.339 kb on - strandat 1192.462 kb on + strandat 1192.462 kb on + strandat 1192.497 kb on + strandat 1192.497 kb on + strandat 1192.497 kb on + strandat 1192.497 kb on + strandat 1192.497 kb on + strandat 1192.497 kb on + strandat 1192.498 kb on - strandat 1192.498 kb on - strandat 1192.498 kb on - strandat 1192.554 kb on + strand, within PFLU_RS05355at 1192.554 kb on + strand, within PFLU_RS05355at 1192.554 kb on + strand, within PFLU_RS05355at 1192.554 kb on + strand, within PFLU_RS05355at 1192.554 kb on + strand, within PFLU_RS05355at 1192.554 kb on + strand, within PFLU_RS05355at 1192.554 kb on + strand, within PFLU_RS05355at 1192.554 kb on + strand, within PFLU_RS05355at 1192.554 kb on + strand, within PFLU_RS05355at 1192.554 kb on + strand, within PFLU_RS05355at 1192.554 kb on + strand, within PFLU_RS05355at 1192.554 kb on + strand, within PFLU_RS05355at 1192.554 kb on + strand, within PFLU_RS05355at 1192.554 kb on + strand, within PFLU_RS05355at 1192.554 kb on + strand, within PFLU_RS05355at 1192.554 kb on + strand, within PFLU_RS05355at 1192.554 kb on + strand, within PFLU_RS05355at 1192.555 kb on - strand, within PFLU_RS05355at 1192.555 kb on - strand, within PFLU_RS05355at 1192.555 kb on - strand, within PFLU_RS05355at 1192.555 kb on - strand, within PFLU_RS05355at 1192.555 kb on - strand, within PFLU_RS05355at 1192.555 kb on - strand, within PFLU_RS05355at 1192.555 kb on - strand, within PFLU_RS05355at 1192.555 kb on - strand, within PFLU_RS05355at 1192.555 kb on - strand, within PFLU_RS05355at 1192.555 kb on - strand, within PFLU_RS05355at 1192.555 kb on - strand, within PFLU_RS05355at 1192.555 kb on - strand, within PFLU_RS05355at 1192.563 kb on + strand, within PFLU_RS05355at 1192.563 kb on + strand, within PFLU_RS05355at 1192.563 kb on + strand, within PFLU_RS05355at 1192.563 kb on + strand, within PFLU_RS05355at 1192.564 kb on - strand, within PFLU_RS05355at 1192.564 kb on - strand, within PFLU_RS05355at 1192.564 kb on - strand, within PFLU_RS05355at 1192.902 kb on - strand, within PFLU_RS05355at 1192.994 kb on + strand, within PFLU_RS05355at 1192.994 kb on + strand, within PFLU_RS05355at 1192.994 kb on + strand, within PFLU_RS05355at 1192.995 kb on - strand, within PFLU_RS05355at 1192.995 kb on - strand, within PFLU_RS05355at 1192.995 kb on - strand, within PFLU_RS05355at 1193.114 kb on + strand, within PFLU_RS05355at 1193.115 kb on - strand, within PFLU_RS05355at 1193.268 kb on + strand, within PFLU_RS05355at 1193.268 kb on + strand, within PFLU_RS05355at 1193.268 kb on + strand, within PFLU_RS05355at 1193.268 kb on + strand, within PFLU_RS05355at 1193.268 kb on + strand, within PFLU_RS05355at 1193.268 kb on + strand, within PFLU_RS05355at 1193.268 kb on + strand, within PFLU_RS05355at 1193.268 kb on + strand, within PFLU_RS05355at 1193.268 kb on + strand, within PFLU_RS05355at 1193.269 kb on - strand, within PFLU_RS05355at 1193.274 kb on + strand, within PFLU_RS05355at 1193.274 kb on + strand, within PFLU_RS05355at 1193.275 kb on - strand, within PFLU_RS05355at 1193.275 kb on - strand, within PFLU_RS05355at 1193.328 kb on + strand, within PFLU_RS05355at 1193.328 kb on + strand, within PFLU_RS05355at 1193.328 kb on + strand, within PFLU_RS05355at 1193.328 kb on + strand, within PFLU_RS05355at 1193.329 kb on - strand, within PFLU_RS05355at 1193.329 kb on - strand, within PFLU_RS05355at 1193.329 kb on - strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.331 kb on + strand, within PFLU_RS05355at 1193.332 kb on - strand, within PFLU_RS05355at 1193.332 kb on - strand, within PFLU_RS05355at 1193.332 kb on - strand, within PFLU_RS05355at 1193.332 kb on - strand, within PFLU_RS05355at 1193.332 kb on - strand, within PFLU_RS05355at 1193.332 kb on - strand, within PFLU_RS05355at 1193.332 kb on - strand, within PFLU_RS05355at 1193.332 kb on - strand, within PFLU_RS05355at 1193.332 kb on - strand, within PFLU_RS05355at 1193.332 kb on - strand, within PFLU_RS05355at 1193.332 kb on - strand, within PFLU_RS05355at 1193.332 kb on - strand, within PFLU_RS05355at 1193.332 kb on - strand, within PFLU_RS05355at 1193.385 kb on + strand, within PFLU_RS05355at 1193.385 kb on + strand, within PFLU_RS05355at 1193.386 kb on - strand, within PFLU_RS05355at 1193.386 kb on - strand, within PFLU_RS05355at 1193.386 kb on - strand, within PFLU_RS05355at 1193.392 kb on - strand, within PFLU_RS05355at 1193.392 kb on - strand, within PFLU_RS05355

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Per-strain Table

Position Strand Gene LocusTag Fraction 4-hydroxyphenylacetic 2 mM (C)
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1,190,716 + PFLU_RS05340 0.62 -0.3
1,190,749 - PFLU_RS05340 0.66 -2.8
1,190,749 - PFLU_RS05340 0.66 -1.9
1,191,012 + -2.0
1,191,012 + -0.3
1,191,012 + +0.5
1,191,012 + -2.5
1,191,012 + +0.4
1,191,013 - -0.7
1,191,013 - +0.4
1,191,013 - -0.9
1,191,013 - -1.2
1,191,176 + PFLU_RS05345 0.54 -0.0
1,191,176 + PFLU_RS05345 0.54 -0.5
1,191,177 - PFLU_RS05345 0.54 -1.6
1,191,177 - PFLU_RS05345 0.54 +1.1
1,191,200 - PFLU_RS05345 0.62 -2.4
1,191,435 + -1.6
1,191,435 + -2.3
1,191,435 + -1.1
1,191,435 + -0.1
1,191,436 - +0.6
1,191,436 - -1.9
1,191,436 - +1.9
1,191,436 - +1.2
1,191,436 - -2.2
1,191,450 + -2.8
1,191,450 + +0.7
1,191,451 - +0.1
1,191,451 - +0.4
1,191,451 - -0.2
1,191,451 - -2.1
1,191,605 + PFLU_RS05350 0.17 -0.6
1,191,605 + PFLU_RS05350 0.17 -1.9
1,191,605 + PFLU_RS05350 0.17 -0.4
1,191,605 + PFLU_RS05350 0.17 -1.9
1,191,605 + PFLU_RS05350 0.17 -2.5
1,191,605 + PFLU_RS05350 0.17 +0.3
1,191,605 + PFLU_RS05350 0.17 -1.6
1,191,605 + PFLU_RS05350 0.17 -1.0
1,191,605 + PFLU_RS05350 0.17 +0.1
1,191,605 + PFLU_RS05350 0.17 -0.1
1,191,605 + PFLU_RS05350 0.17 -0.8
1,191,606 - PFLU_RS05350 0.17 -2.3
1,191,606 - PFLU_RS05350 0.17 +1.9
1,191,606 - PFLU_RS05350 0.17 -1.6
1,191,606 - PFLU_RS05350 0.17 -1.5
1,191,606 - PFLU_RS05350 0.17 -0.4
1,191,606 - PFLU_RS05350 0.17 -0.6
1,191,606 - PFLU_RS05350 0.17 -0.0
1,191,606 - PFLU_RS05350 0.17 -0.9
1,191,606 - PFLU_RS05350 0.17 -1.9
1,191,606 - PFLU_RS05350 0.17 +1.3
1,191,606 - PFLU_RS05350 0.17 +0.7
1,191,899 + PFLU_RS05350 0.47 +2.2
1,191,899 + PFLU_RS05350 0.47 +0.1
1,191,899 + PFLU_RS05350 0.47 -0.1
1,191,900 - PFLU_RS05350 0.48 +0.2
1,191,900 - PFLU_RS05350 0.48 -0.9
1,191,973 - PFLU_RS05350 0.55 +2.0
1,191,973 - PFLU_RS05350 0.55 +1.7
1,191,987 + PFLU_RS05350 0.57 -0.2
1,192,095 + PFLU_RS05350 0.68 +1.2
1,192,096 - PFLU_RS05350 0.68 +1.2
1,192,181 + PFLU_RS05350 0.76 +1.1
1,192,181 + PFLU_RS05350 0.76 +0.2
1,192,181 + PFLU_RS05350 0.76 -1.8
1,192,181 + PFLU_RS05350 0.76 +0.8
1,192,182 - PFLU_RS05350 0.77 -1.1
1,192,182 - PFLU_RS05350 0.77 -0.5
1,192,182 - PFLU_RS05350 0.77 +0.8
1,192,182 - PFLU_RS05350 0.77 -1.3
1,192,339 - -0.5
1,192,462 + -0.2
1,192,462 + +0.5
1,192,497 + -0.5
1,192,497 + +1.0
1,192,497 + +0.4
1,192,497 + +0.8
1,192,497 + +0.3
1,192,497 + +1.4
1,192,498 - -0.4
1,192,498 - -1.0
1,192,498 - -1.2
1,192,554 + PFLU_RS05355 0.12 +0.7
1,192,554 + PFLU_RS05355 0.12 -0.5
1,192,554 + PFLU_RS05355 0.12 -0.4
1,192,554 + PFLU_RS05355 0.12 -1.1
1,192,554 + PFLU_RS05355 0.12 +0.4
1,192,554 + PFLU_RS05355 0.12 +0.7
1,192,554 + PFLU_RS05355 0.12 -1.9
1,192,554 + PFLU_RS05355 0.12 +1.7
1,192,554 + PFLU_RS05355 0.12 +1.5
1,192,554 + PFLU_RS05355 0.12 -1.1
1,192,554 + PFLU_RS05355 0.12 -0.2
1,192,554 + PFLU_RS05355 0.12 -0.1
1,192,554 + PFLU_RS05355 0.12 -1.6
1,192,554 + PFLU_RS05355 0.12 +1.1
1,192,554 + PFLU_RS05355 0.12 -0.6
1,192,554 + PFLU_RS05355 0.12 -1.1
1,192,554 + PFLU_RS05355 0.12 +0.7
1,192,555 - PFLU_RS05355 0.13 +0.7
1,192,555 - PFLU_RS05355 0.13 -0.4
1,192,555 - PFLU_RS05355 0.13 -0.2
1,192,555 - PFLU_RS05355 0.13 +0.2
1,192,555 - PFLU_RS05355 0.13 -1.6
1,192,555 - PFLU_RS05355 0.13 -2.2
1,192,555 - PFLU_RS05355 0.13 +1.0
1,192,555 - PFLU_RS05355 0.13 +0.5
1,192,555 - PFLU_RS05355 0.13 -0.6
1,192,555 - PFLU_RS05355 0.13 -1.9
1,192,555 - PFLU_RS05355 0.13 -0.0
1,192,555 - PFLU_RS05355 0.13 -1.4
1,192,563 + PFLU_RS05355 0.13 -1.5
1,192,563 + PFLU_RS05355 0.13 +0.2
1,192,563 + PFLU_RS05355 0.13 -0.5
1,192,563 + PFLU_RS05355 0.13 +1.0
1,192,564 - PFLU_RS05355 0.13 +0.6
1,192,564 - PFLU_RS05355 0.13 -0.1
1,192,564 - PFLU_RS05355 0.13 -1.3
1,192,902 - PFLU_RS05355 0.42 -0.1
1,192,994 + PFLU_RS05355 0.50 +1.0
1,192,994 + PFLU_RS05355 0.50 +1.7
1,192,994 + PFLU_RS05355 0.50 -0.0
1,192,995 - PFLU_RS05355 0.50 +2.0
1,192,995 - PFLU_RS05355 0.50 -0.3
1,192,995 - PFLU_RS05355 0.50 +0.7
1,193,114 + PFLU_RS05355 0.60 -0.5
1,193,115 - PFLU_RS05355 0.60 -1.3
1,193,268 + PFLU_RS05355 0.73 -2.8
1,193,268 + PFLU_RS05355 0.73 +0.3
1,193,268 + PFLU_RS05355 0.73 -0.5
1,193,268 + PFLU_RS05355 0.73 +2.8
1,193,268 + PFLU_RS05355 0.73 -1.8
1,193,268 + PFLU_RS05355 0.73 +0.8
1,193,268 + PFLU_RS05355 0.73 -0.2
1,193,268 + PFLU_RS05355 0.73 -0.6
1,193,268 + PFLU_RS05355 0.73 -0.6
1,193,269 - PFLU_RS05355 0.73 -2.4
1,193,274 + PFLU_RS05355 0.74 -0.8
1,193,274 + PFLU_RS05355 0.74 -0.9
1,193,275 - PFLU_RS05355 0.74 +0.5
1,193,275 - PFLU_RS05355 0.74 +0.5
1,193,328 + PFLU_RS05355 0.78 -1.3
1,193,328 + PFLU_RS05355 0.78 -1.2
1,193,328 + PFLU_RS05355 0.78 +2.2
1,193,328 + PFLU_RS05355 0.78 +1.1
1,193,329 - PFLU_RS05355 0.78 -0.6
1,193,329 - PFLU_RS05355 0.78 +1.7
1,193,329 - PFLU_RS05355 0.78 -0.4
1,193,331 + PFLU_RS05355 0.79 -0.5
1,193,331 + PFLU_RS05355 0.79 +0.9
1,193,331 + PFLU_RS05355 0.79 +0.4
1,193,331 + PFLU_RS05355 0.79 +1.2
1,193,331 + PFLU_RS05355 0.79 -2.0
1,193,331 + PFLU_RS05355 0.79 +0.4
1,193,331 + PFLU_RS05355 0.79 +0.7
1,193,331 + PFLU_RS05355 0.79 -1.7
1,193,331 + PFLU_RS05355 0.79 +1.7
1,193,331 + PFLU_RS05355 0.79 -2.3
1,193,331 + PFLU_RS05355 0.79 +1.2
1,193,331 + PFLU_RS05355 0.79 +1.1
1,193,331 + PFLU_RS05355 0.79 +1.1
1,193,331 + PFLU_RS05355 0.79 -1.0
1,193,331 + PFLU_RS05355 0.79 -0.4
1,193,331 + PFLU_RS05355 0.79 +0.6
1,193,331 + PFLU_RS05355 0.79 -0.9
1,193,331 + PFLU_RS05355 0.79 -2.9
1,193,331 + PFLU_RS05355 0.79 +1.0
1,193,331 + PFLU_RS05355 0.79 -1.9
1,193,331 + PFLU_RS05355 0.79 -2.5
1,193,331 + PFLU_RS05355 0.79 +1.0
1,193,332 - PFLU_RS05355 0.79 +1.3
1,193,332 - PFLU_RS05355 0.79 +0.1
1,193,332 - PFLU_RS05355 0.79 +2.2
1,193,332 - PFLU_RS05355 0.79 +1.7
1,193,332 - PFLU_RS05355 0.79 -0.4
1,193,332 - PFLU_RS05355 0.79 -2.8
1,193,332 - PFLU_RS05355 0.79 -0.6
1,193,332 - PFLU_RS05355 0.79 -0.8
1,193,332 - PFLU_RS05355 0.79 -0.2
1,193,332 - PFLU_RS05355 0.79 +0.1
1,193,332 - PFLU_RS05355 0.79 +0.7
1,193,332 - PFLU_RS05355 0.79 +0.7
1,193,332 - PFLU_RS05355 0.79 +1.0
1,193,385 + PFLU_RS05355 0.83 +0.2
1,193,385 + PFLU_RS05355 0.83 -0.2
1,193,386 - PFLU_RS05355 0.83 +0.3
1,193,386 - PFLU_RS05355 0.83 -1.1
1,193,386 - PFLU_RS05355 0.83 +1.3
1,193,392 - PFLU_RS05355 0.84 +0.4
1,193,392 - PFLU_RS05355 0.84 +2.2

Or see this region's nucleotide sequence