Experiment: 4-hydroxyphenylacetic 2 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS01165 and PFLU_RS01170 are separated by 40 nucleotides PFLU_RS01170 and PFLU_RS01175 overlap by 11 nucleotides PFLU_RS01175 and PFLU_RS01180 overlap by 4 nucleotides PFLU_RS01180 and PFLU_RS01185 are separated by 81 nucleotides
PFLU_RS01165: PFLU_RS01165 - class I SAM-dependent methyltransferase, at 258,809 to 259,435
_RS01165
PFLU_RS01170: PFLU_RS01170 - ABC transporter permease, at 259,476 to 260,120
_RS01170
PFLU_RS01175: PFLU_RS01175 - ATP-binding cassette domain-containing protein, at 260,110 to 261,225
_RS01175
PFLU_RS01180: PFLU_RS01180 - methionine ABC transporter substrate-binding protein, at 261,222 to 262,037
_RS01180
PFLU_RS01185: PFLU_RS01185 - LLM class flavin-dependent oxidoreductase, at 262,119 to 263,471
_RS01185
Position (kb) 260
261
262 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 259.209 kb on - strand, within PFLU_RS01165 at 259.209 kb on - strand, within PFLU_RS01165 at 259.300 kb on + strand, within PFLU_RS01165 at 259.300 kb on + strand, within PFLU_RS01165 at 259.300 kb on + strand, within PFLU_RS01165 at 259.300 kb on + strand, within PFLU_RS01165 at 259.380 kb on + strand at 259.380 kb on + strand at 259.380 kb on + strand at 259.380 kb on + strand at 259.381 kb on - strand at 259.381 kb on - strand at 259.381 kb on - strand at 259.381 kb on - strand at 259.381 kb on - strand at 259.426 kb on + strand at 259.427 kb on - strand at 259.427 kb on - strand at 259.470 kb on + strand at 259.470 kb on + strand at 259.471 kb on - strand at 259.471 kb on - strand at 259.471 kb on - strand at 259.472 kb on + strand at 259.472 kb on + strand at 259.472 kb on + strand at 259.472 kb on + strand at 259.472 kb on + strand at 259.472 kb on + strand at 259.472 kb on + strand at 259.473 kb on - strand at 259.473 kb on - strand at 259.574 kb on + strand, within PFLU_RS01170 at 259.588 kb on + strand, within PFLU_RS01170 at 259.600 kb on + strand, within PFLU_RS01170 at 259.863 kb on + strand, within PFLU_RS01170 at 259.870 kb on + strand, within PFLU_RS01170 at 259.870 kb on + strand, within PFLU_RS01170 at 259.870 kb on + strand, within PFLU_RS01170 at 259.871 kb on - strand, within PFLU_RS01170 at 259.871 kb on - strand, within PFLU_RS01170 at 259.871 kb on - strand, within PFLU_RS01170 at 259.871 kb on - strand, within PFLU_RS01170 at 259.874 kb on + strand, within PFLU_RS01170 at 259.874 kb on + strand, within PFLU_RS01170 at 259.874 kb on + strand, within PFLU_RS01170 at 259.874 kb on + strand, within PFLU_RS01170 at 259.874 kb on + strand, within PFLU_RS01170 at 259.875 kb on - strand, within PFLU_RS01170 at 259.875 kb on - strand, within PFLU_RS01170 at 259.875 kb on - strand, within PFLU_RS01170 at 259.875 kb on - strand, within PFLU_RS01170 at 259.875 kb on - strand, within PFLU_RS01170 at 259.875 kb on - strand, within PFLU_RS01170 at 259.875 kb on - strand, within PFLU_RS01170 at 259.991 kb on - strand, within PFLU_RS01170 at 259.991 kb on - strand, within PFLU_RS01170 at 259.991 kb on - strand, within PFLU_RS01170 at 259.991 kb on - strand, within PFLU_RS01170 at 259.991 kb on - strand, within PFLU_RS01170 at 260.103 kb on + strand at 260.104 kb on - strand at 260.104 kb on - strand at 260.120 kb on + strand at 260.120 kb on + strand at 260.120 kb on + strand at 260.120 kb on + strand at 260.121 kb on - strand at 260.121 kb on - strand at 260.121 kb on - strand at 260.121 kb on - strand at 260.121 kb on - strand at 260.121 kb on - strand at 260.121 kb on - strand at 260.121 kb on - strand at 260.338 kb on + strand, within PFLU_RS01175 at 260.338 kb on + strand, within PFLU_RS01175 at 260.338 kb on + strand, within PFLU_RS01175 at 260.339 kb on - strand, within PFLU_RS01175 at 260.339 kb on - strand, within PFLU_RS01175 at 260.339 kb on - strand, within PFLU_RS01175 at 260.377 kb on + strand, within PFLU_RS01175 at 260.378 kb on - strand, within PFLU_RS01175 at 260.378 kb on - strand, within PFLU_RS01175 at 260.973 kb on + strand, within PFLU_RS01175 at 260.973 kb on + strand, within PFLU_RS01175 at 260.973 kb on + strand, within PFLU_RS01175 at 260.973 kb on + strand, within PFLU_RS01175 at 260.973 kb on + strand, within PFLU_RS01175 at 260.973 kb on + strand, within PFLU_RS01175 at 260.973 kb on + strand, within PFLU_RS01175 at 260.974 kb on - strand, within PFLU_RS01175 at 260.974 kb on - strand, within PFLU_RS01175 at 260.974 kb on - strand, within PFLU_RS01175 at 261.104 kb on + strand, within PFLU_RS01175 at 261.104 kb on + strand, within PFLU_RS01175 at 261.104 kb on + strand, within PFLU_RS01175 at 261.105 kb on - strand, within PFLU_RS01175 at 261.105 kb on - strand, within PFLU_RS01175 at 261.105 kb on - strand, within PFLU_RS01175 at 261.105 kb on - strand, within PFLU_RS01175 at 261.105 kb on - strand, within PFLU_RS01175 at 261.105 kb on - strand, within PFLU_RS01175 at 261.105 kb on - strand, within PFLU_RS01175 at 261.123 kb on + strand at 261.124 kb on - strand at 261.231 kb on - strand at 261.250 kb on + strand at 261.250 kb on + strand at 261.251 kb on - strand at 261.251 kb on - strand at 261.251 kb on - strand at 261.251 kb on - strand at 261.251 kb on - strand at 261.304 kb on + strand, within PFLU_RS01180 at 261.304 kb on + strand, within PFLU_RS01180 at 261.304 kb on + strand, within PFLU_RS01180 at 261.304 kb on + strand, within PFLU_RS01180 at 261.304 kb on + strand, within PFLU_RS01180 at 261.304 kb on + strand, within PFLU_RS01180 at 261.304 kb on + strand, within PFLU_RS01180 at 261.304 kb on + strand, within PFLU_RS01180 at 261.305 kb on - strand, within PFLU_RS01180 at 261.305 kb on - strand, within PFLU_RS01180 at 261.305 kb on - strand, within PFLU_RS01180 at 261.305 kb on - strand, within PFLU_RS01180 at 261.305 kb on - strand, within PFLU_RS01180 at 261.373 kb on + strand, within PFLU_RS01180 at 261.373 kb on + strand, within PFLU_RS01180 at 261.373 kb on + strand, within PFLU_RS01180 at 261.374 kb on - strand, within PFLU_RS01180 at 261.441 kb on - strand, within PFLU_RS01180 at 261.442 kb on + strand, within PFLU_RS01180 at 261.442 kb on + strand, within PFLU_RS01180 at 261.442 kb on + strand, within PFLU_RS01180 at 261.443 kb on - strand, within PFLU_RS01180 at 261.443 kb on - strand, within PFLU_RS01180 at 261.443 kb on - strand, within PFLU_RS01180 at 261.443 kb on - strand, within PFLU_RS01180 at 261.443 kb on - strand, within PFLU_RS01180 at 261.443 kb on - strand, within PFLU_RS01180 at 261.443 kb on - strand, within PFLU_RS01180 at 261.478 kb on + strand, within PFLU_RS01180 at 261.479 kb on - strand, within PFLU_RS01180 at 261.562 kb on + strand, within PFLU_RS01180 at 261.562 kb on + strand, within PFLU_RS01180 at 261.562 kb on + strand, within PFLU_RS01180 at 261.563 kb on - strand, within PFLU_RS01180 at 261.563 kb on - strand, within PFLU_RS01180 at 261.563 kb on - strand, within PFLU_RS01180 at 261.567 kb on + strand, within PFLU_RS01180 at 261.567 kb on + strand, within PFLU_RS01180 at 261.567 kb on + strand, within PFLU_RS01180 at 261.568 kb on - strand, within PFLU_RS01180 at 261.568 kb on - strand, within PFLU_RS01180 at 261.568 kb on - strand, within PFLU_RS01180 at 261.688 kb on + strand, within PFLU_RS01180 at 261.688 kb on + strand, within PFLU_RS01180 at 261.688 kb on + strand, within PFLU_RS01180 at 261.688 kb on + strand, within PFLU_RS01180 at 261.688 kb on + strand, within PFLU_RS01180 at 261.688 kb on + strand, within PFLU_RS01180 at 261.688 kb on + strand, within PFLU_RS01180 at 261.689 kb on - strand, within PFLU_RS01180 at 261.689 kb on - strand, within PFLU_RS01180 at 261.689 kb on - strand, within PFLU_RS01180 at 261.700 kb on + strand, within PFLU_RS01180 at 261.701 kb on - strand, within PFLU_RS01180 at 261.701 kb on - strand, within PFLU_RS01180 at 261.796 kb on + strand, within PFLU_RS01180 at 261.797 kb on - strand, within PFLU_RS01180 at 261.797 kb on - strand, within PFLU_RS01180 at 261.797 kb on - strand, within PFLU_RS01180 at 261.797 kb on - strand, within PFLU_RS01180 at 261.797 kb on - strand, within PFLU_RS01180 at 261.797 kb on - strand, within PFLU_RS01180 at 262.054 kb on + strand at 262.054 kb on + strand at 262.054 kb on + strand at 262.054 kb on + strand at 262.054 kb on + strand at 262.054 kb on + strand at 262.054 kb on + strand at 262.054 kb on + strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.205 kb on - strand at 262.216 kb on + strand at 262.216 kb on + strand at 262.216 kb on + strand at 262.216 kb on + strand at 262.217 kb on - strand at 262.217 kb on - strand at 262.217 kb on - strand at 262.217 kb on - strand at 262.217 kb on - strand at 262.217 kb on - strand at 262.217 kb on - strand at 262.217 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-hydroxyphenylacetic 2 mM (C) remove 259,209 - PFLU_RS01165 0.64 +0.9 259,209 - PFLU_RS01165 0.64 -0.5 259,300 + PFLU_RS01165 0.78 +0.7 259,300 + PFLU_RS01165 0.78 -0.1 259,300 + PFLU_RS01165 0.78 -1.4 259,300 + PFLU_RS01165 0.78 -3.7 259,380 + +0.8 259,380 + -0.3 259,380 + -0.4 259,380 + -2.0 259,381 - -1.1 259,381 - +0.1 259,381 - +1.8 259,381 - +0.9 259,381 - +1.2 259,426 + -0.6 259,427 - -1.7 259,427 - +1.7 259,470 + -1.1 259,470 + -0.7 259,471 - +0.0 259,471 - +0.9 259,471 - +1.0 259,472 + +0.3 259,472 + +1.6 259,472 + +0.9 259,472 + +1.3 259,472 + -0.2 259,472 + +0.9 259,472 + +0.6 259,473 - -0.8 259,473 - +1.1 259,574 + PFLU_RS01170 0.15 +0.9 259,588 + PFLU_RS01170 0.17 +0.7 259,600 + PFLU_RS01170 0.19 -1.6 259,863 + PFLU_RS01170 0.60 +0.5 259,870 + PFLU_RS01170 0.61 -2.3 259,870 + PFLU_RS01170 0.61 +0.4 259,870 + PFLU_RS01170 0.61 -0.0 259,871 - PFLU_RS01170 0.61 +0.7 259,871 - PFLU_RS01170 0.61 +0.9 259,871 - PFLU_RS01170 0.61 -0.6 259,871 - PFLU_RS01170 0.61 -0.4 259,874 + PFLU_RS01170 0.62 +0.3 259,874 + PFLU_RS01170 0.62 +1.9 259,874 + PFLU_RS01170 0.62 -3.1 259,874 + PFLU_RS01170 0.62 +1.9 259,874 + PFLU_RS01170 0.62 -1.6 259,875 - PFLU_RS01170 0.62 +0.1 259,875 - PFLU_RS01170 0.62 -1.0 259,875 - PFLU_RS01170 0.62 +0.2 259,875 - PFLU_RS01170 0.62 -0.7 259,875 - PFLU_RS01170 0.62 +0.4 259,875 - PFLU_RS01170 0.62 +0.2 259,875 - PFLU_RS01170 0.62 +0.9 259,991 - PFLU_RS01170 0.80 -0.9 259,991 - PFLU_RS01170 0.80 -1.2 259,991 - PFLU_RS01170 0.80 -0.4 259,991 - PFLU_RS01170 0.80 +2.3 259,991 - PFLU_RS01170 0.80 +1.3 260,103 + +1.1 260,104 - +0.9 260,104 - +1.7 260,120 + -0.9 260,120 + -1.2 260,120 + -0.9 260,120 + -0.7 260,121 - -0.4 260,121 - -1.4 260,121 - -1.4 260,121 - -0.3 260,121 - -1.3 260,121 - +1.1 260,121 - +1.2 260,121 - -0.5 260,338 + PFLU_RS01175 0.20 -0.1 260,338 + PFLU_RS01175 0.20 +0.8 260,338 + PFLU_RS01175 0.20 +2.1 260,339 - PFLU_RS01175 0.21 -1.7 260,339 - PFLU_RS01175 0.21 -0.7 260,339 - PFLU_RS01175 0.21 -1.7 260,377 + PFLU_RS01175 0.24 -0.8 260,378 - PFLU_RS01175 0.24 -0.7 260,378 - PFLU_RS01175 0.24 -0.6 260,973 + PFLU_RS01175 0.77 +0.2 260,973 + PFLU_RS01175 0.77 +0.1 260,973 + PFLU_RS01175 0.77 -0.1 260,973 + PFLU_RS01175 0.77 +0.2 260,973 + PFLU_RS01175 0.77 -2.1 260,973 + PFLU_RS01175 0.77 -0.1 260,973 + PFLU_RS01175 0.77 -1.7 260,974 - PFLU_RS01175 0.77 +1.3 260,974 - PFLU_RS01175 0.77 -1.4 260,974 - PFLU_RS01175 0.77 +0.9 261,104 + PFLU_RS01175 0.89 -1.3 261,104 + PFLU_RS01175 0.89 +0.3 261,104 + PFLU_RS01175 0.89 -0.1 261,105 - PFLU_RS01175 0.89 +0.4 261,105 - PFLU_RS01175 0.89 -2.8 261,105 - PFLU_RS01175 0.89 -2.3 261,105 - PFLU_RS01175 0.89 -1.0 261,105 - PFLU_RS01175 0.89 +1.7 261,105 - PFLU_RS01175 0.89 -0.3 261,105 - PFLU_RS01175 0.89 +0.6 261,123 + -0.2 261,124 - -2.6 261,231 - -2.6 261,250 + -0.2 261,250 + +0.1 261,251 - +0.8 261,251 - -1.8 261,251 - -0.5 261,251 - -1.3 261,251 - -0.2 261,304 + PFLU_RS01180 0.10 +0.8 261,304 + PFLU_RS01180 0.10 -1.9 261,304 + PFLU_RS01180 0.10 -0.7 261,304 + PFLU_RS01180 0.10 +0.6 261,304 + PFLU_RS01180 0.10 -0.2 261,304 + PFLU_RS01180 0.10 -0.7 261,304 + PFLU_RS01180 0.10 -0.9 261,304 + PFLU_RS01180 0.10 +0.3 261,305 - PFLU_RS01180 0.10 +0.0 261,305 - PFLU_RS01180 0.10 -1.3 261,305 - PFLU_RS01180 0.10 +0.4 261,305 - PFLU_RS01180 0.10 +0.2 261,305 - PFLU_RS01180 0.10 +0.7 261,373 + PFLU_RS01180 0.19 +1.6 261,373 + PFLU_RS01180 0.19 -0.2 261,373 + PFLU_RS01180 0.19 -0.0 261,374 - PFLU_RS01180 0.19 -1.6 261,441 - PFLU_RS01180 0.27 -0.6 261,442 + PFLU_RS01180 0.27 -0.1 261,442 + PFLU_RS01180 0.27 +0.3 261,442 + PFLU_RS01180 0.27 -0.9 261,443 - PFLU_RS01180 0.27 +0.3 261,443 - PFLU_RS01180 0.27 +0.5 261,443 - PFLU_RS01180 0.27 +0.7 261,443 - PFLU_RS01180 0.27 +0.6 261,443 - PFLU_RS01180 0.27 +0.2 261,443 - PFLU_RS01180 0.27 +0.5 261,443 - PFLU_RS01180 0.27 -0.8 261,478 + PFLU_RS01180 0.31 +0.9 261,479 - PFLU_RS01180 0.31 -0.2 261,562 + PFLU_RS01180 0.42 +0.6 261,562 + PFLU_RS01180 0.42 +1.1 261,562 + PFLU_RS01180 0.42 -1.0 261,563 - PFLU_RS01180 0.42 +0.2 261,563 - PFLU_RS01180 0.42 -0.1 261,563 - PFLU_RS01180 0.42 -2.4 261,567 + PFLU_RS01180 0.42 -2.4 261,567 + PFLU_RS01180 0.42 -0.1 261,567 + PFLU_RS01180 0.42 +1.7 261,568 - PFLU_RS01180 0.42 +1.1 261,568 - PFLU_RS01180 0.42 +0.9 261,568 - PFLU_RS01180 0.42 +0.9 261,688 + PFLU_RS01180 0.57 +0.9 261,688 + PFLU_RS01180 0.57 -0.7 261,688 + PFLU_RS01180 0.57 -0.2 261,688 + PFLU_RS01180 0.57 -2.7 261,688 + PFLU_RS01180 0.57 -0.0 261,688 + PFLU_RS01180 0.57 +1.2 261,688 + PFLU_RS01180 0.57 +0.1 261,689 - PFLU_RS01180 0.57 +0.6 261,689 - PFLU_RS01180 0.57 -0.2 261,689 - PFLU_RS01180 0.57 +2.0 261,700 + PFLU_RS01180 0.59 -1.9 261,701 - PFLU_RS01180 0.59 -0.4 261,701 - PFLU_RS01180 0.59 -0.4 261,796 + PFLU_RS01180 0.70 -0.1 261,797 - PFLU_RS01180 0.70 -0.4 261,797 - PFLU_RS01180 0.70 -1.9 261,797 - PFLU_RS01180 0.70 +0.3 261,797 - PFLU_RS01180 0.70 -0.4 261,797 - PFLU_RS01180 0.70 +1.5 261,797 - PFLU_RS01180 0.70 -1.3 262,054 + +0.3 262,054 + +0.2 262,054 + -1.1 262,054 + -1.3 262,054 + -2.9 262,054 + +1.6 262,054 + +0.2 262,054 + -0.6 262,055 - +0.5 262,055 - -2.1 262,055 - -2.2 262,055 - -2.4 262,055 - -1.2 262,055 - -0.3 262,055 - -1.0 262,055 - +0.2 262,055 - +3.2 262,055 - -1.1 262,205 - -0.4 262,216 + -0.1 262,216 + +1.3 262,216 + +0.5 262,216 + -1.3 262,217 - +0.4 262,217 - -2.9 262,217 - -1.4 262,217 - -2.1 262,217 - +0.4 262,217 - -0.4 262,217 - +0.4 262,217 - -1.9
Or see this region's nucleotide sequence