Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS15945

Experiment: 4-hydroxyphenylacetic 2 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS15935 and PFLU_RS15940 are separated by 45 nucleotidesPFLU_RS15940 and PFLU_RS15945 are separated by 50 nucleotidesPFLU_RS15945 and PFLU_RS15950 are separated by 13 nucleotidesPFLU_RS15950 and PFLU_RS15955 are separated by 14 nucleotides PFLU_RS15935: PFLU_RS15935 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 3,621,836 to 3,622,693 _RS15935 PFLU_RS15940: PFLU_RS15940 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 3,622,739 to 3,623,131 _RS15940 PFLU_RS15945: PFLU_RS15945 - MFS transporter, at 3,623,182 to 3,624,486 _RS15945 PFLU_RS15950: PFLU_RS15950 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase, at 3,624,500 to 3,625,303 _RS15950 PFLU_RS15955: PFLU_RS15955 - 4-hydroxy-2-oxoheptanedioate aldolase, at 3,625,318 to 3,626,121 _RS15955 Position (kb) 3623 3624 3625Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3622.232 kb on + strand, within PFLU_RS15935at 3622.233 kb on - strand, within PFLU_RS15935at 3622.233 kb on - strand, within PFLU_RS15935at 3622.613 kb on + strandat 3622.760 kb on + strandat 3622.760 kb on + strandat 3622.760 kb on + strandat 3622.761 kb on - strandat 3622.761 kb on - strandat 3622.914 kb on - strand, within PFLU_RS15940at 3623.197 kb on - strandat 3623.197 kb on - strandat 3623.281 kb on + strandat 3623.398 kb on + strand, within PFLU_RS15945at 3623.530 kb on + strand, within PFLU_RS15945at 3623.530 kb on + strand, within PFLU_RS15945at 3623.530 kb on + strand, within PFLU_RS15945at 3623.530 kb on + strand, within PFLU_RS15945at 3623.531 kb on - strand, within PFLU_RS15945at 3623.531 kb on - strand, within PFLU_RS15945at 3623.531 kb on - strand, within PFLU_RS15945at 3623.531 kb on - strand, within PFLU_RS15945at 3623.531 kb on - strand, within PFLU_RS15945at 3623.531 kb on - strand, within PFLU_RS15945at 3623.531 kb on - strand, within PFLU_RS15945at 3623.531 kb on - strand, within PFLU_RS15945at 3623.531 kb on - strand, within PFLU_RS15945at 3623.950 kb on + strand, within PFLU_RS15945at 3623.950 kb on + strand, within PFLU_RS15945at 3623.951 kb on - strand, within PFLU_RS15945at 3623.961 kb on - strand, within PFLU_RS15945at 3624.061 kb on + strand, within PFLU_RS15945at 3624.061 kb on + strand, within PFLU_RS15945at 3624.062 kb on - strand, within PFLU_RS15945at 3624.358 kb on + strandat 3624.359 kb on - strandat 3624.359 kb on - strandat 3624.359 kb on - strandat 3624.410 kb on - strandat 3624.410 kb on - strandat 3624.484 kb on + strandat 3624.490 kb on + strandat 3624.490 kb on + strandat 3624.617 kb on + strand, within PFLU_RS15950at 3624.617 kb on + strand, within PFLU_RS15950at 3624.617 kb on + strand, within PFLU_RS15950at 3624.618 kb on - strand, within PFLU_RS15950at 3624.618 kb on - strand, within PFLU_RS15950at 3624.880 kb on - strand, within PFLU_RS15950at 3625.076 kb on + strand, within PFLU_RS15950at 3625.172 kb on + strand, within PFLU_RS15950at 3625.172 kb on + strand, within PFLU_RS15950at 3625.172 kb on + strand, within PFLU_RS15950at 3625.173 kb on - strand, within PFLU_RS15950at 3625.173 kb on - strand, within PFLU_RS15950at 3625.249 kb on + strandat 3625.323 kb on + strandat 3625.324 kb on - strandat 3625.402 kb on + strand, within PFLU_RS15955

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Per-strain Table

Position Strand Gene LocusTag Fraction 4-hydroxyphenylacetic 2 mM (C)
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3,622,232 + PFLU_RS15935 0.46 -0.4
3,622,233 - PFLU_RS15935 0.46 -2.9
3,622,233 - PFLU_RS15935 0.46 -1.4
3,622,613 + -2.0
3,622,760 + -2.4
3,622,760 + -2.4
3,622,760 + -2.8
3,622,761 - -2.3
3,622,761 - -3.3
3,622,914 - PFLU_RS15940 0.45 -1.9
3,623,197 - -1.7
3,623,197 - -2.0
3,623,281 + -2.0
3,623,398 + PFLU_RS15945 0.17 -2.8
3,623,530 + PFLU_RS15945 0.27 -2.0
3,623,530 + PFLU_RS15945 0.27 -1.9
3,623,530 + PFLU_RS15945 0.27 -2.3
3,623,530 + PFLU_RS15945 0.27 -2.3
3,623,531 - PFLU_RS15945 0.27 -3.0
3,623,531 - PFLU_RS15945 0.27 -2.7
3,623,531 - PFLU_RS15945 0.27 -2.2
3,623,531 - PFLU_RS15945 0.27 -3.5
3,623,531 - PFLU_RS15945 0.27 -3.1
3,623,531 - PFLU_RS15945 0.27 -2.6
3,623,531 - PFLU_RS15945 0.27 -2.0
3,623,531 - PFLU_RS15945 0.27 -2.6
3,623,531 - PFLU_RS15945 0.27 -2.0
3,623,950 + PFLU_RS15945 0.59 -1.3
3,623,950 + PFLU_RS15945 0.59 -2.6
3,623,951 - PFLU_RS15945 0.59 -2.0
3,623,961 - PFLU_RS15945 0.60 -1.7
3,624,061 + PFLU_RS15945 0.67 -0.4
3,624,061 + PFLU_RS15945 0.67 -2.0
3,624,062 - PFLU_RS15945 0.67 -0.4
3,624,358 + -1.8
3,624,359 - -1.9
3,624,359 - -2.0
3,624,359 - -2.7
3,624,410 - -3.2
3,624,410 - -1.9
3,624,484 + +0.1
3,624,490 + -4.2
3,624,490 + +0.1
3,624,617 + PFLU_RS15950 0.15 -3.2
3,624,617 + PFLU_RS15950 0.15 +0.1
3,624,617 + PFLU_RS15950 0.15 -2.2
3,624,618 - PFLU_RS15950 0.15 -2.7
3,624,618 - PFLU_RS15950 0.15 -1.7
3,624,880 - PFLU_RS15950 0.47 -1.4
3,625,076 + PFLU_RS15950 0.72 -2.3
3,625,172 + PFLU_RS15950 0.84 -2.2
3,625,172 + PFLU_RS15950 0.84 -2.2
3,625,172 + PFLU_RS15950 0.84 -0.3
3,625,173 - PFLU_RS15950 0.84 -2.3
3,625,173 - PFLU_RS15950 0.84 -2.0
3,625,249 + -2.7
3,625,323 + -1.9
3,625,324 - -1.2
3,625,402 + PFLU_RS15955 0.10 -2.0

Or see this region's nucleotide sequence