Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS29825

Experiment: acetate 30 mM (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	acetate 30 mM (C)
6,627,765	+		PFLU_RS29820	0.37	-1.0
6,627,765	+		PFLU_RS29820	0.37	-1.3
6,627,765	+		PFLU_RS29820	0.37	+1.1
6,627,765	+		PFLU_RS29820	0.37	+0.9
6,627,765	+		PFLU_RS29820	0.37	-1.0
6,627,765	+		PFLU_RS29820	0.37	-1.5
6,627,765	+		PFLU_RS29820	0.37	-1.2
6,627,765	+		PFLU_RS29820	0.37	+1.1
6,627,766	-		PFLU_RS29820	0.37	-0.5
6,627,766	-		PFLU_RS29820	0.37	+2.9
6,627,766	-		PFLU_RS29820	0.37	-0.7
6,627,766	-		PFLU_RS29820	0.37	+1.4
6,627,766	-		PFLU_RS29820	0.37	-0.0
6,627,766	-		PFLU_RS29820	0.37	+0.0
6,627,766	-		PFLU_RS29820	0.37	+0.8
6,627,766	-		PFLU_RS29820	0.37	-0.2
6,627,787	+		PFLU_RS29820	0.38	-0.5
6,627,788	-		PFLU_RS29820	0.38	+0.8
6,627,788	-		PFLU_RS29820	0.38	+1.8
6,627,870	+		PFLU_RS29820	0.44	-2.4
6,627,870	+		PFLU_RS29820	0.44	-0.2
6,627,870	+		PFLU_RS29820	0.44	+0.3
6,627,870	+		PFLU_RS29820	0.44	+1.7
6,627,870	+		PFLU_RS29820	0.44	+1.6
6,627,870	+		PFLU_RS29820	0.44	-1.3
6,627,870	+		PFLU_RS29820	0.44	-1.7
6,627,870	+		PFLU_RS29820	0.44	+0.8
6,627,871	-		PFLU_RS29820	0.44	+0.1
6,627,871	-		PFLU_RS29820	0.44	-2.4
6,627,871	-		PFLU_RS29820	0.44	-1.7
6,627,871	-		PFLU_RS29820	0.44	+1.5
6,627,871	-		PFLU_RS29820	0.44	+0.5
6,627,888	+		PFLU_RS29820	0.45	+0.1
6,627,888	+		PFLU_RS29820	0.45	-1.5
6,627,888	+		PFLU_RS29820	0.45	-1.5
6,627,888	+		PFLU_RS29820	0.45	-2.0
6,627,888	+		PFLU_RS29820	0.45	-2.2
6,627,888	+		PFLU_RS29820	0.45	+1.0
6,627,889	-		PFLU_RS29820	0.45	+0.9
6,627,889	-		PFLU_RS29820	0.45	-2.3
6,627,889	-		PFLU_RS29820	0.45	-1.2
6,627,916	+		PFLU_RS29820	0.47	+0.0
6,627,916	+		PFLU_RS29820	0.47	-0.8
6,627,916	+		PFLU_RS29820	0.47	-1.1
6,628,007	+		PFLU_RS29820	0.53	+0.7
6,628,007	+		PFLU_RS29820	0.53	-2.4
6,628,052	+		PFLU_RS29820	0.56	-3.2
6,628,075	+		PFLU_RS29820	0.58	+0.8
6,628,075	+		PFLU_RS29820	0.58	+0.8
6,628,075	+		PFLU_RS29820	0.58	+0.8
6,628,075	+		PFLU_RS29820	0.58	+0.8
6,628,076	-		PFLU_RS29820	0.58	-1.4
6,628,076	-		PFLU_RS29820	0.58	+2.7
6,628,076	-		PFLU_RS29820	0.58	-1.8
6,628,076	-		PFLU_RS29820	0.58	-2.1
6,628,142	+		PFLU_RS29820	0.63	+1.2
6,628,142	+		PFLU_RS29820	0.63	-1.2
6,628,142	+		PFLU_RS29820	0.63	-1.5
6,628,142	+		PFLU_RS29820	0.63	-1.2
6,628,142	+		PFLU_RS29820	0.63	+1.5
6,628,143	-		PFLU_RS29820	0.63	-0.2
6,628,143	-		PFLU_RS29820	0.63	-1.5
6,628,143	-		PFLU_RS29820	0.63	-1.0
6,628,179	+		PFLU_RS29820	0.65	+0.3
6,628,180	-		PFLU_RS29820	0.65	-2.5
6,628,180	-		PFLU_RS29820	0.65	-2.2
6,628,180	-		PFLU_RS29820	0.65	-0.5
6,628,305	+		PFLU_RS29820	0.74	+0.2
6,628,305	+		PFLU_RS29820	0.74	-1.2
6,628,305	+		PFLU_RS29820	0.74	-1.0
6,628,305	+		PFLU_RS29820	0.74	+0.9
6,628,305	+		PFLU_RS29820	0.74	+1.5
6,628,305	+		PFLU_RS29820	0.74	-0.4
6,628,305	+		PFLU_RS29820	0.74	-1.4
6,628,305	+		PFLU_RS29820	0.74	-2.6
6,628,305	+		PFLU_RS29820	0.74	-0.1
6,628,305	+		PFLU_RS29820	0.74	-0.8
6,628,305	+		PFLU_RS29820	0.74	+1.6
6,628,305	+		PFLU_RS29820	0.74	-1.4
6,628,305	+		PFLU_RS29820	0.74	+3.0
6,628,305	+		PFLU_RS29820	0.74	-0.7
6,628,305	+		PFLU_RS29820	0.74	-2.4
6,628,305	+		PFLU_RS29820	0.74	+1.6
6,628,305	+		PFLU_RS29820	0.74	-2.8
6,628,305	+		PFLU_RS29820	0.74	+1.0
6,628,306	-		PFLU_RS29820	0.74	+1.8
6,628,306	-		PFLU_RS29820	0.74	-0.5
6,628,306	-		PFLU_RS29820	0.74	-1.8
6,628,306	-		PFLU_RS29820	0.74	-0.8
6,628,306	-		PFLU_RS29820	0.74	-1.8
6,628,306	-		PFLU_RS29820	0.74	-1.8
6,628,306	-		PFLU_RS29820	0.74	+0.6
6,628,306	-		PFLU_RS29820	0.74	+2.4
6,628,306	-		PFLU_RS29820	0.74	+0.7
6,628,306	-		PFLU_RS29820	0.74	-2.3
6,628,306	-		PFLU_RS29820	0.74	+1.0
6,628,306	-		PFLU_RS29820	0.74	-0.1
6,628,306	-		PFLU_RS29820	0.74	+3.2
6,628,306	-		PFLU_RS29820	0.74	-0.9
6,628,306	-		PFLU_RS29820	0.74	-2.2
6,628,306	-		PFLU_RS29820	0.74	-0.2
6,628,306	-		PFLU_RS29820	0.74	-1.4
6,628,306	-		PFLU_RS29820	0.74	+0.7
6,628,306	-		PFLU_RS29820	0.74	+0.7
6,628,306	-		PFLU_RS29820	0.74	+0.9
6,628,306	-		PFLU_RS29820	0.74	-2.4
6,628,306	-		PFLU_RS29820	0.74	-0.6
6,628,311	+		PFLU_RS29820	0.74	+0.5
6,628,311	+		PFLU_RS29820	0.74	+3.0
6,628,311	+		PFLU_RS29820	0.74	-0.8
6,628,311	+		PFLU_RS29820	0.74	-0.2
6,628,311	+		PFLU_RS29820	0.74	-2.4
6,628,311	+		PFLU_RS29820	0.74	-1.2
6,628,311	+		PFLU_RS29820	0.74	-0.3
6,628,311	+		PFLU_RS29820	0.74	+0.4
6,628,311	+		PFLU_RS29820	0.74	+0.2
6,628,311	+		PFLU_RS29820	0.74	-1.5
6,628,311	+		PFLU_RS29820	0.74	+0.6
6,628,311	+		PFLU_RS29820	0.74	+1.4
6,628,311	+		PFLU_RS29820	0.74	-3.0
6,628,311	+		PFLU_RS29820	0.74	-2.9
6,628,311	+		PFLU_RS29820	0.74	+2.6
6,628,311	+		PFLU_RS29820	0.74	-1.5
6,628,311	+		PFLU_RS29820	0.74	+0.8
6,628,311	+		PFLU_RS29820	0.74	-0.5
6,628,311	+		PFLU_RS29820	0.74	-0.4
6,628,311	+		PFLU_RS29820	0.74	+3.7
6,628,311	+		PFLU_RS29820	0.74	-2.3
6,628,311	+		PFLU_RS29820	0.74	-1.2
6,628,311	+		PFLU_RS29820	0.74	-1.0
6,628,311	+		PFLU_RS29820	0.74	-1.9
6,628,311	+		PFLU_RS29820	0.74	-2.5
6,628,311	+		PFLU_RS29820	0.74	-1.3
6,628,311	+		PFLU_RS29820	0.74	-0.6
6,628,311	+		PFLU_RS29820	0.74	-0.3
6,628,311	+		PFLU_RS29820	0.74	-0.3
6,628,311	+		PFLU_RS29820	0.74	-1.7
6,628,311	+		PFLU_RS29820	0.74	-1.9
6,628,311	+		PFLU_RS29820	0.74	-2.2
6,628,311	+		PFLU_RS29820	0.74	-0.7
6,628,311	+		PFLU_RS29820	0.74	-0.9
6,628,311	+		PFLU_RS29820	0.74	-0.7
6,628,311	+		PFLU_RS29820	0.74	+0.2
6,628,311	+		PFLU_RS29820	0.74	-1.5
6,628,311	+		PFLU_RS29820	0.74	+0.4
6,628,312	-		PFLU_RS29820	0.74	+0.8
6,628,312	-		PFLU_RS29820	0.74	-0.6
6,628,312	-		PFLU_RS29820	0.74	-1.1
6,628,312	-		PFLU_RS29820	0.74	-0.2
6,628,312	-		PFLU_RS29820	0.74	-0.4
6,628,312	-		PFLU_RS29820	0.74	+1.0
6,628,312	-		PFLU_RS29820	0.74	-1.2
6,628,312	-		PFLU_RS29820	0.74	-2.1
6,628,312	-		PFLU_RS29820	0.74	-4.0
6,628,312	-		PFLU_RS29820	0.74	+0.8
6,628,312	-		PFLU_RS29820	0.74	-1.5
6,628,312	-		PFLU_RS29820	0.74	-1.2
6,628,312	-		PFLU_RS29820	0.74	+0.8
6,628,312	-		PFLU_RS29820	0.74	-2.1
6,628,312	-		PFLU_RS29820	0.74	-1.4
6,628,312	-		PFLU_RS29820	0.74	+2.4
6,628,312	-		PFLU_RS29820	0.74	-0.9
6,628,312	-		PFLU_RS29820	0.74	+1.5
6,628,312	-		PFLU_RS29820	0.74	-2.0
6,628,312	-		PFLU_RS29820	0.74	-2.7
6,628,312	-		PFLU_RS29820	0.74	+1.1
6,628,312	-		PFLU_RS29820	0.74	+0.4
6,628,312	-		PFLU_RS29820	0.74	+1.6
6,628,312	-		PFLU_RS29820	0.74	+2.0
6,628,312	-		PFLU_RS29820	0.74	-0.7
6,628,312	-		PFLU_RS29820	0.74	+0.2
6,628,312	-		PFLU_RS29820	0.74	-1.5
6,628,312	-		PFLU_RS29820	0.74	-1.7
6,628,312	-		PFLU_RS29820	0.74	-1.0
6,628,314	+		PFLU_RS29820	0.74	+0.6
6,628,314	+		PFLU_RS29820	0.74	-0.8
6,628,314	+		PFLU_RS29820	0.74	+0.4
6,628,314	+		PFLU_RS29820	0.74	-1.5
6,628,314	+		PFLU_RS29820	0.74	-0.7
6,628,315	-		PFLU_RS29820	0.75	+0.7
6,628,349	+		PFLU_RS29820	0.77	+2.2
6,628,686	+				+1.2
6,628,686	+				+2.8
6,628,686	+				-2.3
6,628,686	+				-1.7
6,628,686	+				-1.5
6,628,686	+				+0.1
6,628,686	+				-2.0
6,628,686	+				-1.0
6,628,686	+				+0.2
6,628,686	+				+2.2
6,628,686	+				+0.3
6,628,686	+				-2.3
6,628,686	+				-1.4
6,628,686	+				-0.3
6,628,686	+				+2.4
6,628,687	-				-1.5
6,628,687	-				+0.8
6,628,687	-				+0.1
6,628,687	-				+0.6
6,628,690	+				+1.4
6,628,690	+				-0.2
6,628,690	+				+0.8
6,628,690	+				+2.4
6,628,690	+				-0.2
6,628,690	+				-0.2
6,628,690	+				+2.0
6,628,690	+				+0.8
6,628,690	+				+1.5
6,628,690	+				+1.8
6,628,690	+				+3.2
6,628,690	+				+0.2
6,628,690	+				-1.9
6,628,690	+				-1.1
6,628,690	+				-1.9
6,628,690	+				+1.2
6,628,690	+				-1.4
6,628,690	+				-3.2
6,628,690	+				-1.8
6,628,690	+				-0.5
6,628,691	-				+2.3
6,628,691	-				+1.4
6,628,691	-				+0.8
6,628,691	-				-0.7
6,628,691	-				-2.3
6,628,691	-				-2.5
6,628,691	-				-0.2
6,628,691	-				-3.0
6,628,691	-				-0.3
6,628,691	-				+0.4
6,628,691	-				-0.1
6,628,691	-				+0.6
6,628,691	-				+0.6
6,628,691	-				-1.1
6,628,691	-				+0.2
6,628,691	-				+2.9
6,628,691	-				+1.3
6,628,691	-				+0.8
6,628,691	-				+0.3
6,628,691	-				+0.2
6,628,794	+				-2.2
6,628,794	+				+0.2
6,628,794	+				-1.3
6,628,794	+				+0.0
6,628,795	-				-0.5
6,628,795	-				+0.8
6,628,795	-				-1.8
6,628,795	-				-1.8
6,628,795	-				+1.4
6,628,795	-				-0.2
6,628,795	-				-0.5
6,628,956	+		PFLU_RS29825	0.20	-1.4
6,628,956	+		PFLU_RS29825	0.20	-0.2
6,629,014	+		PFLU_RS29825	0.26	-2.1
6,629,015	-		PFLU_RS29825	0.26	-1.2
6,629,015	-		PFLU_RS29825	0.26	-3.0
6,629,015	-		PFLU_RS29825	0.26	-2.0
6,629,015	-		PFLU_RS29825	0.26	-0.2
6,629,015	-		PFLU_RS29825	0.26	-2.8
6,629,046	+		PFLU_RS29825	0.29	-0.5
6,629,046	+		PFLU_RS29825	0.29	+1.8
6,629,046	+		PFLU_RS29825	0.29	+1.9
6,629,046	+		PFLU_RS29825	0.29	-0.6
6,629,208	+		PFLU_RS29825	0.46	-1.4
6,629,208	+		PFLU_RS29825	0.46	+2.8
6,629,208	+		PFLU_RS29825	0.46	+2.0
6,629,208	+		PFLU_RS29825	0.46	-1.8
6,629,208	+		PFLU_RS29825	0.46	-1.2
6,629,208	+		PFLU_RS29825	0.46	-1.8
6,629,208	+		PFLU_RS29825	0.46	+0.8
6,629,208	+		PFLU_RS29825	0.46	-1.8
6,629,208	+		PFLU_RS29825	0.46	-2.5
6,629,208	+		PFLU_RS29825	0.46	-0.4
6,629,208	+		PFLU_RS29825	0.46	-0.8
6,629,209	-		PFLU_RS29825	0.46	+2.8
6,629,209	-		PFLU_RS29825	0.46	+0.2
6,629,209	-		PFLU_RS29825	0.46	-1.0
6,629,209	-		PFLU_RS29825	0.46	+1.8
6,629,209	-		PFLU_RS29825	0.46	-0.0
6,629,209	-		PFLU_RS29825	0.46	+1.1
6,629,580	+		PFLU_RS29825	0.83	-2.0
6,629,580	+		PFLU_RS29825	0.83	-1.0
6,629,581	-		PFLU_RS29825	0.84	-0.5
6,629,614	+		PFLU_RS29825	0.87	+1.4
6,629,614	+		PFLU_RS29825	0.87	+0.7
6,629,615	-		PFLU_RS29825	0.87	+0.3
6,629,748	+				-0.2
6,629,748	+				-0.2
6,629,748	+				+0.7
6,629,748	+				+0.5
6,629,748	+				-1.4
6,629,748	+				-0.0
6,629,748	+				+1.3
6,629,749	-				+0.2
6,629,749	-				+1.2
6,629,749	-				+1.2
6,629,749	-				+0.4
6,629,749	-				+1.9
6,629,749	-				-1.9
6,629,750	+				+0.2
6,629,750	+				+1.8
6,629,750	+				+1.0
6,629,750	+				-0.5
6,629,750	+				+0.2
6,629,750	+				+1.5
6,629,750	+				+0.1
6,629,750	+				+0.9
6,629,750	+				+3.4
6,629,750	+				+0.8
6,629,750	+				-1.4
6,629,750	+				+0.8
6,629,750	+				+1.8
6,629,750	+				-2.3
6,629,750	+				+0.9
6,629,750	+				+1.0
6,629,750	+				+0.2
6,629,750	+				-1.7
6,629,750	+				+0.4
6,629,751	-				+4.1
6,629,751	-				+2.0
6,629,751	-				+1.6
6,629,751	-				-2.1
6,629,751	-				-0.6
6,629,751	-				+0.4
6,629,751	-				+1.8
6,629,751	-				-0.1
6,629,751	-				+0.4
6,629,751	-				-1.4
6,629,751	-				+0.6
6,629,751	-				+0.2
6,629,751	-				-0.2
6,629,751	-				+2.1
6,629,751	-				-0.5
6,629,751	-				+0.6
6,629,751	-				+0.2
6,629,751	-				-1.8
6,629,751	-				+0.1
6,629,751	-				+0.2
6,629,751	-				-2.0
6,629,751	-				+2.1
6,629,751	-				-1.0
6,629,751	-				+0.8
6,629,751	-				-2.7
6,629,751	-				+0.4
6,629,751	-				+1.1
6,629,751	-				+2.5
6,629,843	+				-2.6
6,629,843	+				+0.7
6,629,844	-				+1.3
6,629,894	+		PFLU_RS29830	0.10	+1.3
6,629,895	-		PFLU_RS29830	0.10	+0.6
6,630,019	+		PFLU_RS29830	0.23	-0.8
6,630,019	+		PFLU_RS29830	0.23	-2.3
6,630,019	+		PFLU_RS29830	0.23	+1.1
6,630,019	+		PFLU_RS29830	0.23	+0.9
6,630,019	+		PFLU_RS29830	0.23	-0.5
6,630,019	+		PFLU_RS29830	0.23	-2.2
6,630,019	+		PFLU_RS29830	0.23	-2.0
6,630,019	+		PFLU_RS29830	0.23	+1.4
6,630,019	+		PFLU_RS29830	0.23	-0.4
6,630,019	+		PFLU_RS29830	0.23	+1.8
6,630,019	+		PFLU_RS29830	0.23	-2.5
6,630,019	+		PFLU_RS29830	0.23	+1.4
6,630,019	+		PFLU_RS29830	0.23	-1.6
6,630,019	+		PFLU_RS29830	0.23	+1.0
6,630,019	+		PFLU_RS29830	0.23	+0.1
6,630,019	+		PFLU_RS29830	0.23	+0.4
6,630,019	+		PFLU_RS29830	0.23	+1.3
6,630,019	+		PFLU_RS29830	0.23	+0.8
6,630,019	+		PFLU_RS29830	0.23	-1.2
6,630,019	+		PFLU_RS29830	0.23	-1.2
6,630,019	+		PFLU_RS29830	0.23	+0.2
6,630,019	+		PFLU_RS29830	0.23	+1.3
6,630,019	+		PFLU_RS29830	0.23	+0.4
6,630,019	+		PFLU_RS29830	0.23	+1.2
6,630,019	+		PFLU_RS29830	0.23	+1.5
6,630,019	+		PFLU_RS29830	0.23	-0.4
6,630,019	+		PFLU_RS29830	0.23	+0.2
6,630,020	-		PFLU_RS29830	0.23	-0.7
6,630,020	-		PFLU_RS29830	0.23	+2.3
6,630,020	-		PFLU_RS29830	0.23	-1.3
6,630,020	-		PFLU_RS29830	0.23	-0.3
6,630,020	-		PFLU_RS29830	0.23	+0.7
6,630,020	-		PFLU_RS29830	0.23	+3.2
6,630,020	-		PFLU_RS29830	0.23	+1.1
6,630,020	-		PFLU_RS29830	0.23	-2.4
6,630,020	-		PFLU_RS29830	0.23	+0.1
6,630,020	-		PFLU_RS29830	0.23	-0.1
6,630,020	-		PFLU_RS29830	0.23	+1.1
6,630,020	-		PFLU_RS29830	0.23	-1.3
6,630,020	-		PFLU_RS29830	0.23	-0.0
6,630,020	-		PFLU_RS29830	0.23	+1.0
6,630,020	-		PFLU_RS29830	0.23	-0.6
6,630,020	-		PFLU_RS29830	0.23	-0.5
6,630,020	-		PFLU_RS29830	0.23	+0.1
6,630,020	-		PFLU_RS29830	0.23	-3.2
6,630,020	-		PFLU_RS29830	0.23	+1.6
6,630,020	-		PFLU_RS29830	0.23	-1.8
6,630,020	-		PFLU_RS29830	0.23	+1.8
6,630,020	-		PFLU_RS29830	0.23	-1.2
6,630,020	-		PFLU_RS29830	0.23	-0.9
6,630,020	-		PFLU_RS29830	0.23	+0.9
6,630,020	-		PFLU_RS29830	0.23	-1.7
6,630,020	-		PFLU_RS29830	0.23	-1.1
6,630,020	-		PFLU_RS29830	0.23	+2.0
6,630,020	-		PFLU_RS29830	0.23	-0.9
6,630,020	-		PFLU_RS29830	0.23	-0.7
6,630,020	-		PFLU_RS29830	0.23	+0.8
6,630,020	-		PFLU_RS29830	0.23	-0.2
6,630,027	+		PFLU_RS29830	0.24	-0.0
6,630,042	+		PFLU_RS29830	0.25	+1.1
6,630,042	+		PFLU_RS29830	0.25	+1.5
6,630,042	+		PFLU_RS29830	0.25	-1.0
6,630,152	+		PFLU_RS29830	0.36	-1.2
6,630,152	+		PFLU_RS29830	0.36	-1.8
6,630,152	+		PFLU_RS29830	0.36	+2.2
6,630,152	+		PFLU_RS29830	0.36	+0.8
6,630,152	+		PFLU_RS29830	0.36	-2.7
6,630,152	+		PFLU_RS29830	0.36	+3.6
6,630,152	+		PFLU_RS29830	0.36	+1.3
6,630,152	+		PFLU_RS29830	0.36	-0.2
6,630,152	+		PFLU_RS29830	0.36	-1.1
6,630,153	-		PFLU_RS29830	0.36	-0.2
6,630,153	-		PFLU_RS29830	0.36	-0.1
6,630,153	-		PFLU_RS29830	0.36	-1.9
6,630,153	-		PFLU_RS29830	0.36	-1.5
6,630,153	-		PFLU_RS29830	0.36	-1.4
6,630,153	-		PFLU_RS29830	0.36	+0.9
6,630,153	-		PFLU_RS29830	0.36	-1.8
6,630,306	+		PFLU_RS29830	0.52	-1.0
6,630,319	-		PFLU_RS29830	0.53	-0.0
6,630,615	+		PFLU_RS29830	0.83	+2.0
6,630,615	+		PFLU_RS29830	0.83	-0.5
6,630,616	-		PFLU_RS29830	0.83	+1.5
6,630,616	-		PFLU_RS29830	0.83	+1.9
6,630,616	-		PFLU_RS29830	0.83	-2.8
6,630,616	-		PFLU_RS29830	0.83	+0.1
6,630,616	-		PFLU_RS29830	0.83	+0.8
6,630,687	+				+1.3
6,630,688	-				+2.1
6,630,688	-				+1.5