Experiment: L-Valine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS25915 and PFLU_RS25920 are separated by 98 nucleotides PFLU_RS25920 and PFLU_RS25925 are separated by 63 nucleotides PFLU_RS25925 and PFLU_RS25930 overlap by 8 nucleotides PFLU_RS25930 and PFLU_RS25935 are separated by 168 nucleotides
PFLU_RS25915: PFLU_RS25915 - ferric iron uptake transcriptional regulator, at 5,789,671 to 5,790,075
_RS25915
PFLU_RS25920: PFLU_RS25920 - outer membrane protein assembly factor BamE, at 5,790,174 to 5,790,701
_RS25920
PFLU_RS25925: PFLU_RS25925 - RnfH family protein, at 5,790,765 to 5,791,067
_RS25925
PFLU_RS25930: PFLU_RS25930 - type II toxin-antitoxin system RatA family toxin, at 5,791,060 to 5,791,494
_RS25930
PFLU_RS25935: PFLU_RS25935 - SsrA-binding protein SmpB, at 5,791,663 to 5,792,145
_RS25935
Position (kb) 5790
5791
5792 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 5790.115 kb on - strand at 5790.124 kb on - strand at 5790.124 kb on - strand at 5790.124 kb on - strand at 5790.155 kb on + strand at 5790.155 kb on + strand at 5790.548 kb on + strand, within PFLU_RS25920 at 5790.548 kb on + strand, within PFLU_RS25920 at 5790.595 kb on + strand, within PFLU_RS25920 at 5790.595 kb on + strand, within PFLU_RS25920 at 5790.596 kb on - strand, within PFLU_RS25920 at 5790.596 kb on - strand, within PFLU_RS25920 at 5790.700 kb on - strand at 5790.700 kb on - strand at 5790.766 kb on + strand at 5790.767 kb on - strand at 5790.767 kb on - strand at 5790.767 kb on - strand at 5790.767 kb on - strand at 5790.857 kb on + strand, within PFLU_RS25925 at 5790.857 kb on + strand, within PFLU_RS25925 at 5790.858 kb on - strand, within PFLU_RS25925 at 5790.858 kb on - strand, within PFLU_RS25925 at 5790.963 kb on - strand, within PFLU_RS25925 at 5791.045 kb on + strand at 5791.045 kb on + strand at 5791.045 kb on + strand at 5791.045 kb on + strand at 5791.045 kb on + strand at 5791.045 kb on + strand at 5791.045 kb on + strand at 5791.045 kb on + strand at 5791.045 kb on + strand at 5791.045 kb on + strand at 5791.045 kb on + strand at 5791.046 kb on - strand at 5791.046 kb on - strand at 5791.046 kb on - strand at 5791.046 kb on - strand at 5791.504 kb on - strand at 5791.504 kb on - strand at 5791.504 kb on - strand at 5791.648 kb on + strand at 5791.648 kb on + strand at 5791.648 kb on + strand at 5791.648 kb on + strand at 5791.648 kb on + strand at 5791.648 kb on + strand at 5791.648 kb on + strand at 5791.648 kb on + strand at 5791.648 kb on + strand at 5791.648 kb on + strand at 5791.649 kb on - strand at 5791.649 kb on - strand at 5791.650 kb on + strand at 5791.650 kb on + strand at 5791.650 kb on + strand at 5791.650 kb on + strand at 5791.668 kb on + strand at 5791.778 kb on + strand, within PFLU_RS25935 at 5791.819 kb on + strand, within PFLU_RS25935 at 5791.819 kb on + strand, within PFLU_RS25935 at 5791.820 kb on - strand, within PFLU_RS25935
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine 10 mM (C) remove 5,790,115 - -0.4 5,790,124 - +1.2 5,790,124 - +0.2 5,790,124 - +2.2 5,790,155 + -0.4 5,790,155 + +2.2 5,790,548 + PFLU_RS25920 0.71 +1.9 5,790,548 + PFLU_RS25920 0.71 +2.3 5,790,595 + PFLU_RS25920 0.80 +1.8 5,790,595 + PFLU_RS25920 0.80 +2.0 5,790,596 - PFLU_RS25920 0.80 +3.2 5,790,596 - PFLU_RS25920 0.80 +2.1 5,790,700 - -0.6 5,790,700 - +4.1 5,790,766 + -1.6 5,790,767 - -2.0 5,790,767 - +0.7 5,790,767 - +0.6 5,790,767 - -1.6 5,790,857 + PFLU_RS25925 0.30 +0.6 5,790,857 + PFLU_RS25925 0.30 -1.1 5,790,858 - PFLU_RS25925 0.31 +1.2 5,790,858 - PFLU_RS25925 0.31 -0.9 5,790,963 - PFLU_RS25925 0.65 -1.8 5,791,045 + -2.3 5,791,045 + -0.2 5,791,045 + +0.2 5,791,045 + +3.3 5,791,045 + -1.0 5,791,045 + -1.6 5,791,045 + -0.3 5,791,045 + -2.0 5,791,045 + -1.9 5,791,045 + +0.4 5,791,045 + -0.6 5,791,046 - +0.9 5,791,046 - -0.2 5,791,046 - +0.6 5,791,046 - +2.4 5,791,504 - +0.4 5,791,504 - +0.9 5,791,504 - -0.4 5,791,648 + +1.3 5,791,648 + +0.3 5,791,648 + -0.5 5,791,648 + +0.9 5,791,648 + -0.1 5,791,648 + +0.6 5,791,648 + -1.2 5,791,648 + +0.6 5,791,648 + -1.0 5,791,648 + +0.4 5,791,649 - +1.2 5,791,649 - +2.2 5,791,650 + +0.6 5,791,650 + -0.4 5,791,650 + +4.6 5,791,650 + +2.2 5,791,668 + +2.2 5,791,778 + PFLU_RS25935 0.24 +1.2 5,791,819 + PFLU_RS25935 0.32 +1.2 5,791,819 + PFLU_RS25935 0.32 +3.0 5,791,820 - PFLU_RS25935 0.33 +0.6
Or see this region's nucleotide sequence