Experiment: HNL05_cps7_1MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2177 and BT2178 are separated by 292 nucleotides BT2178 and BT2179 are separated by 129 nucleotides BT2179 and BT2180 are separated by 126 nucleotides BT2180 and BT2181 are separated by 40 nucleotides
BT2177: BT2177 - putative membrane protein (NCBI ptt file), at 2,736,386 to 2,736,994
BT2177
BT2178: BT2178 - hypothetical protein (NCBI ptt file), at 2,737,287 to 2,737,643
BT2178
BT2179: BT2179 - putative DNA mismatch repair protein (NCBI ptt file), at 2,737,773 to 2,738,837
BT2179
BT2180: BT2180 - conserved hypothetical protein (NCBI ptt file), at 2,738,964 to 2,739,659
BT2180
BT2181: BT2181 - transcriptional regulator (NCBI ptt file), at 2,739,700 to 2,740,473
BT2181
Position (kb)
2737
2738
2739 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2736.787 kb on - strand, within BT2177 at 2736.838 kb on + strand, within BT2177 at 2736.872 kb on - strand, within BT2177 at 2737.230 kb on + strand at 2737.252 kb on + strand at 2737.265 kb on + strand at 2737.284 kb on - strand at 2737.284 kb on - strand at 2737.286 kb on + strand at 2737.361 kb on + strand, within BT2178 at 2737.397 kb on + strand, within BT2178 at 2737.421 kb on - strand, within BT2178 at 2737.506 kb on - strand, within BT2178 at 2737.534 kb on - strand, within BT2178 at 2737.587 kb on + strand, within BT2178 at 2737.588 kb on - strand, within BT2178 at 2737.608 kb on - strand at 2737.608 kb on - strand at 2737.612 kb on + strand at 2737.638 kb on - strand at 2737.641 kb on + strand at 2737.655 kb on - strand at 2737.655 kb on - strand at 2738.009 kb on + strand, within BT2179 at 2738.030 kb on + strand, within BT2179 at 2738.030 kb on + strand, within BT2179 at 2738.109 kb on - strand, within BT2179 at 2738.161 kb on + strand, within BT2179 at 2738.161 kb on + strand, within BT2179 at 2738.161 kb on + strand, within BT2179 at 2738.162 kb on - strand, within BT2179 at 2738.162 kb on - strand, within BT2179 at 2738.163 kb on + strand, within BT2179 at 2738.164 kb on - strand, within BT2179 at 2738.164 kb on - strand, within BT2179 at 2738.196 kb on - strand, within BT2179 at 2738.243 kb on + strand, within BT2179 at 2738.255 kb on - strand, within BT2179 at 2738.368 kb on - strand, within BT2179 at 2738.391 kb on + strand, within BT2179 at 2738.461 kb on + strand, within BT2179 at 2738.462 kb on - strand, within BT2179 at 2738.465 kb on + strand, within BT2179 at 2738.467 kb on + strand, within BT2179 at 2738.479 kb on + strand, within BT2179 at 2738.548 kb on + strand, within BT2179 at 2738.548 kb on + strand, within BT2179 at 2738.548 kb on + strand, within BT2179 at 2738.676 kb on + strand, within BT2179 at 2738.683 kb on + strand, within BT2179 at 2738.715 kb on + strand, within BT2179 at 2738.716 kb on - strand, within BT2179 at 2738.757 kb on + strand at 2738.800 kb on - strand at 2738.822 kb on + strand at 2738.843 kb on + strand at 2738.843 kb on + strand at 2738.923 kb on + strand at 2738.986 kb on + strand at 2739.072 kb on + strand, within BT2180 at 2739.073 kb on - strand, within BT2180 at 2739.122 kb on + strand, within BT2180 at 2739.122 kb on + strand, within BT2180 at 2739.123 kb on - strand at 2739.184 kb on + strand, within BT2180 at 2739.339 kb on - strand, within BT2180 at 2739.339 kb on - strand, within BT2180 at 2739.442 kb on - strand, within BT2180 at 2739.459 kb on - strand, within BT2180 at 2739.529 kb on + strand, within BT2180 at 2739.535 kb on + strand, within BT2180 at 2739.550 kb on + strand, within BT2180 at 2739.554 kb on + strand, within BT2180 at 2739.649 kb on + strand at 2739.658 kb on - strand at 2739.662 kb on - strand at 2739.662 kb on - strand at 2739.662 kb on - strand at 2739.662 kb on - strand at 2739.666 kb on + strand at 2739.666 kb on + strand at 2739.666 kb on + strand at 2739.698 kb on + strand at 2739.796 kb on - strand, within BT2181
Per-strain Table
Position Strand Gene LocusTag Fraction HNL05_cps7_1MOI remove 2,736,787 - BT2177 0.66 -1.1 2,736,838 + BT2177 0.74 +0.6 2,736,872 - BT2177 0.80 -0.8 2,737,230 + -3.5 2,737,252 + -0.6 2,737,265 + -1.9 2,737,284 - -2.5 2,737,284 - +1.7 2,737,286 + +2.1 2,737,361 + BT2178 0.21 -0.8 2,737,397 + BT2178 0.31 +1.2 2,737,421 - BT2178 0.38 +0.4 2,737,506 - BT2178 0.61 -1.4 2,737,534 - BT2178 0.69 +0.0 2,737,587 + BT2178 0.84 -0.8 2,737,588 - BT2178 0.84 +0.2 2,737,608 - -1.1 2,737,608 - -3.2 2,737,612 + -0.5 2,737,638 - -0.8 2,737,641 + -0.2 2,737,655 - +0.2 2,737,655 - +0.2 2,738,009 + BT2179 0.22 -1.1 2,738,030 + BT2179 0.24 +0.4 2,738,030 + BT2179 0.24 +0.8 2,738,109 - BT2179 0.32 +0.2 2,738,161 + BT2179 0.36 -1.9 2,738,161 + BT2179 0.36 +0.5 2,738,161 + BT2179 0.36 +1.0 2,738,162 - BT2179 0.37 -3.3 2,738,162 - BT2179 0.37 -2.1 2,738,163 + BT2179 0.37 -1.6 2,738,164 - BT2179 0.37 +0.4 2,738,164 - BT2179 0.37 -1.9 2,738,196 - BT2179 0.40 +1.2 2,738,243 + BT2179 0.44 +2.0 2,738,255 - BT2179 0.45 -0.8 2,738,368 - BT2179 0.56 -1.9 2,738,391 + BT2179 0.58 -0.6 2,738,461 + BT2179 0.65 +0.8 2,738,462 - BT2179 0.65 -1.8 2,738,465 + BT2179 0.65 +0.8 2,738,467 + BT2179 0.65 +1.2 2,738,479 + BT2179 0.66 -0.9 2,738,548 + BT2179 0.73 -1.6 2,738,548 + BT2179 0.73 +0.2 2,738,548 + BT2179 0.73 -3.0 2,738,676 + BT2179 0.85 +0.6 2,738,683 + BT2179 0.85 +0.1 2,738,715 + BT2179 0.88 -0.3 2,738,716 - BT2179 0.89 -0.3 2,738,757 + -1.3 2,738,800 - -0.1 2,738,822 + -0.3 2,738,843 + -0.0 2,738,843 + -0.8 2,738,923 + +0.0 2,738,986 + -0.9 2,739,072 + BT2180 0.16 -1.2 2,739,073 - BT2180 0.16 -0.0 2,739,122 + BT2180 0.23 +1.2 2,739,122 + BT2180 0.23 -3.4 2,739,123 - +0.2 2,739,184 + BT2180 0.32 -1.3 2,739,339 - BT2180 0.54 +0.2 2,739,339 - BT2180 0.54 +0.7 2,739,442 - BT2180 0.69 -1.1 2,739,459 - BT2180 0.71 +0.7 2,739,529 + BT2180 0.81 -1.8 2,739,535 + BT2180 0.82 -2.3 2,739,550 + BT2180 0.84 -0.8 2,739,554 + BT2180 0.85 -0.6 2,739,649 + -0.2 2,739,658 - -3.1 2,739,662 - -1.6 2,739,662 - +0.2 2,739,662 - -1.3 2,739,662 - -1.6 2,739,666 + -2.7 2,739,666 + -1.1 2,739,666 + -0.8 2,739,698 + -1.1 2,739,796 - BT2181 0.12 +0.3
Or see this region's nucleotide sequence