Experiment: HNL05_cps7_1MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1553 and BT1554 are separated by 105 nucleotides BT1554 and BT1555 are separated by 84 nucleotides
BT1553: BT1553 - hypothetical protein (NCBI ptt file), at 1,920,134 to 1,921,462
BT1553
BT1554: BT1554 - alanine dehydrogenase (NCBI ptt file), at 1,921,568 to 1,922,674
BT1554
BT1555: BT1555 - dioxygenase, related to 2-nitropropane dioxygenase (NCBI ptt file), at 1,922,759 to 1,923,694
BT1555
Position (kb)
1921
1922
1923 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1920.570 kb on + strand, within BT1553 at 1920.584 kb on + strand, within BT1553 at 1920.586 kb on + strand, within BT1553 at 1920.586 kb on + strand, within BT1553 at 1920.652 kb on - strand, within BT1553 at 1920.681 kb on - strand, within BT1553 at 1920.681 kb on - strand, within BT1553 at 1920.681 kb on - strand, within BT1553 at 1920.683 kb on - strand, within BT1553 at 1920.683 kb on - strand, within BT1553 at 1920.693 kb on + strand, within BT1553 at 1920.702 kb on + strand, within BT1553 at 1920.702 kb on + strand, within BT1553 at 1920.749 kb on + strand, within BT1553 at 1920.749 kb on + strand, within BT1553 at 1920.830 kb on + strand, within BT1553 at 1920.831 kb on - strand, within BT1553 at 1920.831 kb on - strand, within BT1553 at 1920.852 kb on + strand, within BT1553 at 1920.852 kb on + strand, within BT1553 at 1920.912 kb on - strand, within BT1553 at 1920.932 kb on - strand, within BT1553 at 1920.981 kb on + strand, within BT1553 at 1921.009 kb on + strand, within BT1553 at 1921.009 kb on + strand, within BT1553 at 1921.009 kb on + strand, within BT1553 at 1921.054 kb on + strand, within BT1553 at 1921.055 kb on - strand, within BT1553 at 1921.059 kb on - strand, within BT1553 at 1921.091 kb on - strand, within BT1553 at 1921.182 kb on + strand, within BT1553 at 1921.330 kb on - strand at 1921.330 kb on - strand at 1921.336 kb on + strand at 1921.379 kb on + strand at 1921.379 kb on + strand at 1921.379 kb on + strand at 1921.380 kb on - strand at 1921.413 kb on + strand at 1921.470 kb on - strand at 1921.470 kb on - strand at 1921.473 kb on + strand at 1921.509 kb on - strand at 1921.558 kb on - strand at 1921.561 kb on + strand at 1921.568 kb on - strand at 1921.665 kb on + strand at 1921.665 kb on + strand at 1921.666 kb on - strand at 1921.667 kb on + strand at 1921.667 kb on + strand at 1921.668 kb on - strand at 1921.669 kb on + strand at 1921.669 kb on + strand at 1921.669 kb on + strand at 1921.715 kb on + strand, within BT1554 at 1921.716 kb on - strand, within BT1554 at 1921.716 kb on - strand, within BT1554 at 1921.774 kb on + strand, within BT1554 at 1921.836 kb on + strand, within BT1554 at 1922.165 kb on - strand, within BT1554 at 1922.223 kb on - strand, within BT1554 at 1922.326 kb on + strand, within BT1554 at 1922.329 kb on - strand, within BT1554 at 1922.355 kb on + strand, within BT1554 at 1922.425 kb on + strand, within BT1554 at 1922.425 kb on + strand, within BT1554 at 1922.426 kb on - strand, within BT1554 at 1922.436 kb on + strand, within BT1554 at 1922.437 kb on - strand, within BT1554 at 1922.453 kb on + strand, within BT1554 at 1922.454 kb on - strand, within BT1554 at 1922.489 kb on - strand, within BT1554 at 1922.526 kb on - strand, within BT1554 at 1922.545 kb on + strand, within BT1554 at 1922.601 kb on + strand at 1922.601 kb on + strand at 1922.621 kb on + strand at 1922.622 kb on - strand at 1922.703 kb on - strand at 1922.705 kb on + strand at 1922.706 kb on - strand at 1923.003 kb on - strand, within BT1555 at 1923.003 kb on - strand, within BT1555 at 1923.067 kb on - strand, within BT1555 at 1923.128 kb on + strand, within BT1555 at 1923.163 kb on + strand, within BT1555 at 1923.359 kb on + strand, within BT1555 at 1923.367 kb on + strand, within BT1555 at 1923.400 kb on + strand, within BT1555 at 1923.478 kb on - strand, within BT1555 at 1923.656 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HNL05_cps7_1MOI remove 1,920,570 + BT1553 0.33 -1.4 1,920,584 + BT1553 0.34 -2.9 1,920,586 + BT1553 0.34 -0.8 1,920,586 + BT1553 0.34 -0.4 1,920,652 - BT1553 0.39 -2.8 1,920,681 - BT1553 0.41 -1.4 1,920,681 - BT1553 0.41 +1.2 1,920,681 - BT1553 0.41 -2.2 1,920,683 - BT1553 0.41 -1.7 1,920,683 - BT1553 0.41 -1.5 1,920,693 + BT1553 0.42 +0.2 1,920,702 + BT1553 0.43 -0.9 1,920,702 + BT1553 0.43 -0.1 1,920,749 + BT1553 0.46 -2.4 1,920,749 + BT1553 0.46 -0.8 1,920,830 + BT1553 0.52 +1.2 1,920,831 - BT1553 0.52 +1.9 1,920,831 - BT1553 0.52 -1.7 1,920,852 + BT1553 0.54 +0.2 1,920,852 + BT1553 0.54 -3.0 1,920,912 - BT1553 0.59 -2.9 1,920,932 - BT1553 0.60 -2.0 1,920,981 + BT1553 0.64 -2.2 1,921,009 + BT1553 0.66 +0.2 1,921,009 + BT1553 0.66 -1.5 1,921,009 + BT1553 0.66 -0.7 1,921,054 + BT1553 0.69 -0.7 1,921,055 - BT1553 0.69 -0.7 1,921,059 - BT1553 0.70 -2.0 1,921,091 - BT1553 0.72 -1.2 1,921,182 + BT1553 0.79 +1.2 1,921,330 - -1.7 1,921,330 - -1.4 1,921,336 + -1.0 1,921,379 + -0.3 1,921,379 + -2.2 1,921,379 + +1.2 1,921,380 - -2.7 1,921,413 + -1.3 1,921,470 - -0.9 1,921,470 - -2.6 1,921,473 + -0.3 1,921,509 - +1.5 1,921,558 - -2.7 1,921,561 + -1.2 1,921,568 - -1.1 1,921,665 + +0.1 1,921,665 + -2.1 1,921,666 - +1.6 1,921,667 + -2.4 1,921,667 + -1.4 1,921,668 - -1.0 1,921,669 + -1.1 1,921,669 + -2.0 1,921,669 + -3.0 1,921,715 + BT1554 0.13 -0.7 1,921,716 - BT1554 0.13 -2.5 1,921,716 - BT1554 0.13 -2.0 1,921,774 + BT1554 0.19 -1.3 1,921,836 + BT1554 0.24 -2.0 1,922,165 - BT1554 0.54 +0.1 1,922,223 - BT1554 0.59 -1.7 1,922,326 + BT1554 0.68 -0.4 1,922,329 - BT1554 0.69 -2.2 1,922,355 + BT1554 0.71 -0.9 1,922,425 + BT1554 0.77 -2.4 1,922,425 + BT1554 0.77 -1.8 1,922,426 - BT1554 0.78 -0.7 1,922,436 + BT1554 0.78 -3.0 1,922,437 - BT1554 0.79 -1.2 1,922,453 + BT1554 0.80 -1.4 1,922,454 - BT1554 0.80 -1.4 1,922,489 - BT1554 0.83 -0.9 1,922,526 - BT1554 0.87 -2.8 1,922,545 + BT1554 0.88 -1.9 1,922,601 + -2.8 1,922,601 + -0.8 1,922,621 + +1.0 1,922,622 - +0.1 1,922,703 - +0.4 1,922,705 + -1.9 1,922,706 - -1.4 1,923,003 - BT1555 0.26 -1.7 1,923,003 - BT1555 0.26 -3.2 1,923,067 - BT1555 0.33 -1.9 1,923,128 + BT1555 0.39 -2.2 1,923,163 + BT1555 0.43 +1.4 1,923,359 + BT1555 0.64 +0.1 1,923,367 + BT1555 0.65 -2.0 1,923,400 + BT1555 0.68 -1.1 1,923,478 - BT1555 0.77 -2.0 1,923,656 - +0.1
Or see this region's nucleotide sequence