Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS18635

Experiment: 4-Hydroxybenzoic Acid 2 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS18625 and PFLU_RS18630 are separated by 2 nucleotidesPFLU_RS18630 and PFLU_RS18635 overlap by 4 nucleotidesPFLU_RS18635 and PFLU_RS18640 are separated by 136 nucleotides PFLU_RS18625: PFLU_RS18625 - NADH-quinone oxidoreductase subunit C/D, at 4,214,925 to 4,216,709 _RS18625 PFLU_RS18630: PFLU_RS18630 - NADH-quinone oxidoreductase subunit NuoE, at 4,216,712 to 4,217,209 _RS18630 PFLU_RS18635: PFLU_RS18635 - NADH-quinone oxidoreductase subunit NuoF, at 4,217,206 to 4,218,561 _RS18635 PFLU_RS18640: PFLU_RS18640 - NADH-quinone oxidoreductase subunit NuoG, at 4,218,698 to 4,221,412 _RS18640 Position (kb) 4217 4218 4219Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4216.297 kb on - strand, within PFLU_RS18625at 4216.297 kb on - strand, within PFLU_RS18625at 4216.383 kb on + strand, within PFLU_RS18625at 4216.383 kb on + strand, within PFLU_RS18625at 4216.384 kb on - strand, within PFLU_RS18625at 4216.542 kb on + strandat 4216.542 kb on + strandat 4216.543 kb on - strandat 4216.543 kb on - strandat 4216.543 kb on - strandat 4216.546 kb on - strandat 4216.546 kb on - strandat 4216.576 kb on - strandat 4216.674 kb on - strandat 4216.707 kb on + strandat 4216.794 kb on - strand, within PFLU_RS18630at 4216.875 kb on - strand, within PFLU_RS18630at 4216.935 kb on - strand, within PFLU_RS18630at 4217.001 kb on - strand, within PFLU_RS18630at 4217.485 kb on + strand, within PFLU_RS18635at 4217.614 kb on + strand, within PFLU_RS18635at 4217.636 kb on - strand, within PFLU_RS18635at 4217.636 kb on - strand, within PFLU_RS18635at 4217.761 kb on + strand, within PFLU_RS18635at 4217.762 kb on - strand, within PFLU_RS18635at 4217.763 kb on + strand, within PFLU_RS18635at 4217.763 kb on + strand, within PFLU_RS18635at 4217.763 kb on + strand, within PFLU_RS18635at 4217.763 kb on + strand, within PFLU_RS18635at 4217.763 kb on + strand, within PFLU_RS18635at 4217.763 kb on + strand, within PFLU_RS18635at 4217.764 kb on - strand, within PFLU_RS18635at 4217.764 kb on - strand, within PFLU_RS18635at 4218.088 kb on + strand, within PFLU_RS18635at 4218.164 kb on - strand, within PFLU_RS18635at 4218.164 kb on - strand, within PFLU_RS18635at 4218.476 kb on - strandat 4218.548 kb on + strandat 4218.548 kb on + strandat 4218.549 kb on - strandat 4218.549 kb on - strandat 4218.566 kb on + strandat 4218.566 kb on + strandat 4218.641 kb on + strandat 4218.641 kb on + strandat 4218.641 kb on + strandat 4218.642 kb on - strandat 4218.715 kb on - strandat 4218.757 kb on - strandat 4218.757 kb on - strandat 4218.854 kb on + strandat 4218.855 kb on - strandat 4218.883 kb on + strandat 4219.180 kb on + strand, within PFLU_RS18640at 4219.181 kb on - strand, within PFLU_RS18640at 4219.188 kb on - strand, within PFLU_RS18640at 4219.213 kb on - strand, within PFLU_RS18640at 4219.235 kb on + strand, within PFLU_RS18640at 4219.236 kb on - strand, within PFLU_RS18640at 4219.340 kb on + strand, within PFLU_RS18640at 4219.348 kb on + strand, within PFLU_RS18640at 4219.348 kb on + strand, within PFLU_RS18640at 4219.349 kb on - strand, within PFLU_RS18640at 4219.475 kb on + strand, within PFLU_RS18640at 4219.476 kb on - strand, within PFLU_RS18640at 4219.512 kb on - strand, within PFLU_RS18640

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Per-strain Table

Position Strand Gene LocusTag Fraction 4-Hydroxybenzoic Acid 2 mM (C)
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4,216,297 - PFLU_RS18625 0.77 +1.2
4,216,297 - PFLU_RS18625 0.77 -0.4
4,216,383 + PFLU_RS18625 0.82 +1.2
4,216,383 + PFLU_RS18625 0.82 +1.2
4,216,384 - PFLU_RS18625 0.82 +0.2
4,216,542 + +0.2
4,216,542 + +0.2
4,216,543 - +1.2
4,216,543 - +1.2
4,216,543 - -0.4
4,216,546 - -0.8
4,216,546 - +1.2
4,216,576 - +3.2
4,216,674 - -1.1
4,216,707 + -0.4
4,216,794 - PFLU_RS18630 0.16 -0.4
4,216,875 - PFLU_RS18630 0.33 -1.1
4,216,935 - PFLU_RS18630 0.45 +1.2
4,217,001 - PFLU_RS18630 0.58 +0.2
4,217,485 + PFLU_RS18635 0.21 -0.4
4,217,614 + PFLU_RS18635 0.30 +1.2
4,217,636 - PFLU_RS18635 0.32 +0.2
4,217,636 - PFLU_RS18635 0.32 -0.4
4,217,761 + PFLU_RS18635 0.41 +0.2
4,217,762 - PFLU_RS18635 0.41 +1.2
4,217,763 + PFLU_RS18635 0.41 +1.2
4,217,763 + PFLU_RS18635 0.41 -0.4
4,217,763 + PFLU_RS18635 0.41 +0.2
4,217,763 + PFLU_RS18635 0.41 -0.4
4,217,763 + PFLU_RS18635 0.41 -1.1
4,217,763 + PFLU_RS18635 0.41 +0.2
4,217,764 - PFLU_RS18635 0.41 +1.2
4,217,764 - PFLU_RS18635 0.41 +1.2
4,218,088 + PFLU_RS18635 0.65 +1.2
4,218,164 - PFLU_RS18635 0.71 +0.2
4,218,164 - PFLU_RS18635 0.71 -0.8
4,218,476 - +0.2
4,218,548 + -2.0
4,218,548 + +0.4
4,218,549 - +2.8
4,218,549 - +0.2
4,218,566 + -2.0
4,218,566 + +0.6
4,218,641 + +0.2
4,218,641 + +1.8
4,218,641 + -0.1
4,218,642 - +0.2
4,218,715 - +0.2
4,218,757 - -0.8
4,218,757 - -0.8
4,218,854 + +1.2
4,218,855 - +0.2
4,218,883 + +0.2
4,219,180 + PFLU_RS18640 0.18 +1.2
4,219,181 - PFLU_RS18640 0.18 +1.2
4,219,188 - PFLU_RS18640 0.18 -0.4
4,219,213 - PFLU_RS18640 0.19 +1.2
4,219,235 + PFLU_RS18640 0.20 +1.2
4,219,236 - PFLU_RS18640 0.20 +0.2
4,219,340 + PFLU_RS18640 0.24 -0.4
4,219,348 + PFLU_RS18640 0.24 +1.2
4,219,348 + PFLU_RS18640 0.24 -0.8
4,219,349 - PFLU_RS18640 0.24 +1.2
4,219,475 + PFLU_RS18640 0.29 -0.8
4,219,476 - PFLU_RS18640 0.29 +0.2
4,219,512 - PFLU_RS18640 0.30 +0.2

Or see this region's nucleotide sequence