Experiment: DAC20_cps8_1MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1075 and BT1076 overlap by 8 nucleotides BT1076 and tRNA-Asp are separated by 137 nucleotides tRNA-Asp and BT1077 are separated by 157 nucleotides
BT1075: BT1075 - putative two-component system sensor protein, but no histidine kinase domain (NCBI ptt file), at 1,361,784 to 1,362,821
BT1075
BT1076: BT1076 - two-component system response regulator (NCBI ptt file), at 1,362,814 to 1,363,593
BT1076
BT_t19: tRNA-Asp - tRNA-Asp (RefSeq), at 1,363,731 to 1,363,804
tRNA-Asp
BT1077: BT1077 - putative aspartate aminotransferase (NCBI ptt file), at 1,363,962 to 1,365,278
BT1077
Position (kb)
1363
1364 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1362.761 kb on + strand at 1362.764 kb on + strand at 1362.876 kb on + strand at 1362.876 kb on + strand at 1362.882 kb on - strand at 1362.914 kb on + strand, within BT1076 at 1363.092 kb on + strand, within BT1076 at 1363.115 kb on + strand, within BT1076 at 1363.132 kb on - strand, within BT1076 at 1363.256 kb on - strand, within BT1076 at 1363.328 kb on - strand, within BT1076 at 1363.454 kb on + strand, within BT1076 at 1363.541 kb on + strand at 1363.562 kb on + strand at 1363.625 kb on + strand at 1363.886 kb on - strand at 1364.244 kb on - strand, within BT1077 at 1364.304 kb on - strand, within BT1077 at 1364.410 kb on + strand, within BT1077 at 1364.504 kb on + strand, within BT1077 at 1364.505 kb on - strand, within BT1077 at 1364.517 kb on - strand, within BT1077 at 1364.712 kb on + strand, within BT1077 at 1364.749 kb on - strand, within BT1077
Per-strain Table
Position Strand Gene LocusTag Fraction DAC20_cps8_1MOI remove 1,362,761 + +1.4 1,362,764 + -0.0 1,362,876 + +1.4 1,362,876 + -0.6 1,362,882 - -1.0 1,362,914 + BT1076 0.13 +0.4 1,363,092 + BT1076 0.36 -2.7 1,363,115 + BT1076 0.39 +0.7 1,363,132 - BT1076 0.41 -2.6 1,363,256 - BT1076 0.57 -0.4 1,363,328 - BT1076 0.66 -0.9 1,363,454 + BT1076 0.82 -1.8 1,363,541 + +0.4 1,363,562 + -0.6 1,363,625 + +0.7 1,363,886 - -1.3 1,364,244 - BT1077 0.21 -3.4 1,364,304 - BT1077 0.26 -0.9 1,364,410 + BT1077 0.34 -2.4 1,364,504 + BT1077 0.41 -0.3 1,364,505 - BT1077 0.41 -2.2 1,364,517 - BT1077 0.42 -0.0 1,364,712 + BT1077 0.57 +1.4 1,364,749 - BT1077 0.60 +1.0
Or see this region's nucleotide sequence