Experiment: DAC20_cps8_1MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2322 and BT2323 are separated by 697 nucleotides BT2323 and BT2324 are separated by 26 nucleotides
BT2322: BT2322 - putative integrase (NCBI ptt file), at 2,897,656 to 2,898,654
BT2322
BT2323: BT2323 - conserved hypothetical protein (NCBI ptt file), at 2,899,352 to 2,900,680
BT2323
BT2324: BT2324 - DNA topoisomerase III (NCBI ptt file), at 2,900,707 to 2,902,806
BT2324
Position (kb)
2899
2900
2901 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2898.369 kb on + strand, within BT2322 at 2898.369 kb on + strand, within BT2322 at 2898.369 kb on + strand, within BT2322 at 2898.407 kb on - strand, within BT2322 at 2898.450 kb on + strand, within BT2322 at 2898.451 kb on - strand, within BT2322 at 2898.451 kb on - strand, within BT2322 at 2898.480 kb on + strand, within BT2322 at 2898.480 kb on + strand, within BT2322 at 2898.482 kb on + strand, within BT2322 at 2898.482 kb on + strand, within BT2322 at 2898.485 kb on - strand, within BT2322 at 2898.485 kb on - strand, within BT2322 at 2898.493 kb on - strand, within BT2322 at 2898.510 kb on + strand, within BT2322 at 2898.542 kb on + strand, within BT2322 at 2898.543 kb on - strand, within BT2322 at 2898.552 kb on - strand, within BT2322 at 2898.613 kb on - strand at 2898.628 kb on + strand at 2898.628 kb on + strand at 2898.634 kb on + strand at 2898.655 kb on - strand at 2898.661 kb on - strand at 2898.661 kb on - strand at 2898.687 kb on - strand at 2898.691 kb on - strand at 2898.742 kb on + strand at 2898.750 kb on - strand at 2898.833 kb on + strand at 2898.833 kb on + strand at 2898.836 kb on + strand at 2898.894 kb on - strand at 2898.901 kb on + strand at 2898.902 kb on - strand at 2898.905 kb on - strand at 2898.912 kb on + strand at 2898.913 kb on - strand at 2898.913 kb on - strand at 2898.927 kb on + strand at 2898.957 kb on - strand at 2898.960 kb on - strand at 2898.969 kb on + strand at 2899.051 kb on - strand at 2899.141 kb on + strand at 2899.196 kb on - strand at 2899.215 kb on - strand at 2899.294 kb on - strand at 2899.317 kb on - strand at 2899.609 kb on - strand, within BT2323 at 2899.676 kb on + strand, within BT2323 at 2899.677 kb on - strand, within BT2323 at 2899.992 kb on - strand, within BT2323 at 2900.290 kb on + strand, within BT2323 at 2900.385 kb on + strand, within BT2323 at 2900.386 kb on - strand, within BT2323 at 2900.386 kb on - strand, within BT2323 at 2900.459 kb on - strand, within BT2323 at 2900.491 kb on - strand, within BT2323 at 2900.671 kb on - strand at 2900.691 kb on - strand at 2900.694 kb on + strand at 2900.698 kb on - strand at 2900.702 kb on + strand at 2900.702 kb on + strand at 2900.703 kb on - strand at 2900.703 kb on - strand at 2900.792 kb on + strand at 2900.792 kb on + strand at 2900.950 kb on + strand, within BT2324 at 2901.073 kb on + strand, within BT2324 at 2901.090 kb on + strand, within BT2324 at 2901.091 kb on - strand, within BT2324 at 2901.179 kb on - strand, within BT2324 at 2901.365 kb on - strand, within BT2324 at 2901.484 kb on - strand, within BT2324 at 2901.504 kb on - strand, within BT2324 at 2901.641 kb on + strand, within BT2324 at 2901.641 kb on + strand, within BT2324 at 2901.647 kb on - strand, within BT2324 at 2901.669 kb on + strand, within BT2324 at 2901.669 kb on + strand, within BT2324 at 2901.669 kb on + strand, within BT2324 at 2901.670 kb on - strand at 2901.670 kb on - strand, within BT2324
Per-strain Table
Position Strand Gene LocusTag Fraction DAC20_cps8_1MOI remove 2,898,369 + BT2322 0.71 +0.3 2,898,369 + BT2322 0.71 +0.6 2,898,369 + BT2322 0.71 -1.0 2,898,407 - BT2322 0.75 -1.4 2,898,450 + BT2322 0.79 -2.7 2,898,451 - BT2322 0.80 -1.8 2,898,451 - BT2322 0.80 -3.2 2,898,480 + BT2322 0.82 +0.7 2,898,480 + BT2322 0.82 +1.3 2,898,482 + BT2322 0.83 -0.4 2,898,482 + BT2322 0.83 -0.2 2,898,485 - BT2322 0.83 -0.6 2,898,485 - BT2322 0.83 -2.0 2,898,493 - BT2322 0.84 -2.1 2,898,510 + BT2322 0.85 -2.9 2,898,542 + BT2322 0.89 -1.8 2,898,543 - BT2322 0.89 -1.4 2,898,552 - BT2322 0.90 -2.8 2,898,613 - +1.0 2,898,628 + -0.6 2,898,628 + -0.6 2,898,634 + -1.0 2,898,655 - -0.0 2,898,661 - -0.3 2,898,661 - -0.0 2,898,687 - -2.3 2,898,691 - -0.8 2,898,742 + -1.2 2,898,750 - -0.4 2,898,833 + +1.2 2,898,833 + -1.3 2,898,836 + -0.4 2,898,894 - -1.0 2,898,901 + -0.6 2,898,902 - -0.6 2,898,905 - -0.0 2,898,912 + +0.1 2,898,913 - -0.3 2,898,913 - -1.7 2,898,927 + -1.3 2,898,957 - -0.2 2,898,960 - -3.2 2,898,969 + -3.2 2,899,051 - -2.6 2,899,141 + +1.0 2,899,196 - -1.5 2,899,215 - +0.1 2,899,294 - -0.0 2,899,317 - -2.0 2,899,609 - BT2323 0.19 -0.6 2,899,676 + BT2323 0.24 -2.3 2,899,677 - BT2323 0.24 -1.7 2,899,992 - BT2323 0.48 -1.0 2,900,290 + BT2323 0.71 -1.8 2,900,385 + BT2323 0.78 -1.6 2,900,386 - BT2323 0.78 -3.9 2,900,386 - BT2323 0.78 -1.4 2,900,459 - BT2323 0.83 -2.2 2,900,491 - BT2323 0.86 +0.2 2,900,671 - -2.0 2,900,691 - -2.6 2,900,694 + +0.6 2,900,698 - -2.8 2,900,702 + -0.3 2,900,702 + -0.2 2,900,703 - +0.2 2,900,703 - -0.7 2,900,792 + -0.0 2,900,792 + -2.2 2,900,950 + BT2324 0.12 +0.4 2,901,073 + BT2324 0.17 -2.5 2,901,090 + BT2324 0.18 -1.6 2,901,091 - BT2324 0.18 +1.0 2,901,179 - BT2324 0.22 +0.4 2,901,365 - BT2324 0.31 -2.0 2,901,484 - BT2324 0.37 -0.7 2,901,504 - BT2324 0.38 -2.3 2,901,641 + BT2324 0.44 -2.0 2,901,641 + BT2324 0.44 -0.6 2,901,647 - BT2324 0.45 -0.8 2,901,669 + BT2324 0.46 -1.1 2,901,669 + BT2324 0.46 -2.0 2,901,669 + BT2324 0.46 +1.0 2,901,670 - -1.0 2,901,670 - BT2324 0.46 -0.0
Or see this region's nucleotide sequence