Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_542 and Pf6N2E2_543 are separated by 450 nucleotides Pf6N2E2_543 and Pf6N2E2_544 are separated by 153 nucleotides Pf6N2E2_544 and Pf6N2E2_545 are separated by 24 nucleotides
Pf6N2E2_542 - hypothetical protein, at 610,330 to 611,763
_542
Pf6N2E2_543 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 612,214 to 613,329
_543
Pf6N2E2_544 - Short-chain dehydrogenase/reductase SDR, at 613,483 to 614,250
_544
Pf6N2E2_545 - Predicted aminoglycoside phosphotransferase, at 614,275 to 615,342
_545
Position (kb) 612
613
614 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 611.282 kb on + strand, within Pf6N2E2_542 at 611.282 kb on + strand, within Pf6N2E2_542 at 611.282 kb on + strand, within Pf6N2E2_542 at 611.282 kb on + strand, within Pf6N2E2_542 at 611.282 kb on + strand, within Pf6N2E2_542 at 611.282 kb on + strand, within Pf6N2E2_542 at 611.283 kb on - strand, within Pf6N2E2_542 at 611.283 kb on - strand, within Pf6N2E2_542 at 611.283 kb on - strand, within Pf6N2E2_542 at 611.283 kb on - strand, within Pf6N2E2_542 at 611.504 kb on - strand, within Pf6N2E2_542 at 611.811 kb on + strand at 611.811 kb on + strand at 612.215 kb on + strand at 612.216 kb on - strand at 612.217 kb on + strand at 612.217 kb on + strand at 612.217 kb on + strand at 612.217 kb on + strand at 612.217 kb on + strand at 612.217 kb on + strand at 612.217 kb on + strand at 612.217 kb on + strand at 612.218 kb on - strand at 612.218 kb on - strand at 612.218 kb on - strand at 612.218 kb on - strand at 612.218 kb on - strand at 612.218 kb on - strand at 612.378 kb on - strand, within Pf6N2E2_543 at 612.378 kb on - strand, within Pf6N2E2_543 at 612.648 kb on + strand, within Pf6N2E2_543 at 612.648 kb on + strand, within Pf6N2E2_543 at 612.648 kb on + strand, within Pf6N2E2_543 at 612.648 kb on + strand, within Pf6N2E2_543 at 612.648 kb on + strand, within Pf6N2E2_543 at 612.648 kb on + strand, within Pf6N2E2_543 at 612.648 kb on + strand, within Pf6N2E2_543 at 612.649 kb on - strand, within Pf6N2E2_543 at 612.649 kb on - strand, within Pf6N2E2_543 at 612.649 kb on - strand, within Pf6N2E2_543 at 612.935 kb on + strand, within Pf6N2E2_543 at 612.935 kb on + strand, within Pf6N2E2_543 at 612.936 kb on - strand, within Pf6N2E2_543 at 612.936 kb on - strand, within Pf6N2E2_543 at 612.936 kb on - strand, within Pf6N2E2_543 at 612.941 kb on + strand, within Pf6N2E2_543 at 612.941 kb on + strand, within Pf6N2E2_543 at 612.942 kb on - strand, within Pf6N2E2_543 at 613.031 kb on + strand, within Pf6N2E2_543 at 613.032 kb on - strand, within Pf6N2E2_543 at 613.444 kb on + strand at 613.444 kb on + strand at 613.445 kb on - strand at 613.445 kb on - strand at 613.445 kb on - strand at 613.445 kb on - strand at 613.445 kb on - strand at 613.445 kb on - strand at 613.529 kb on + strand at 613.529 kb on + strand at 613.553 kb on + strand at 613.553 kb on + strand at 613.554 kb on - strand at 613.769 kb on + strand, within Pf6N2E2_544 at 613.769 kb on + strand, within Pf6N2E2_544 at 613.769 kb on + strand, within Pf6N2E2_544 at 613.770 kb on - strand, within Pf6N2E2_544 at 613.773 kb on + strand, within Pf6N2E2_544 at 613.774 kb on - strand, within Pf6N2E2_544 at 614.266 kb on + strand at 614.266 kb on + strand at 614.266 kb on + strand at 614.266 kb on + strand at 614.266 kb on + strand at 614.266 kb on + strand at 614.266 kb on + strand at 614.267 kb on - strand at 614.267 kb on - strand at 614.267 kb on - strand at 614.267 kb on - strand at 614.267 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 611,282 + Pf6N2E2_542 0.66 +1.5 611,282 + Pf6N2E2_542 0.66 -0.4 611,282 + Pf6N2E2_542 0.66 -1.4 611,282 + Pf6N2E2_542 0.66 +0.7 611,282 + Pf6N2E2_542 0.66 -1.3 611,282 + Pf6N2E2_542 0.66 -1.2 611,283 - Pf6N2E2_542 0.66 +0.5 611,283 - Pf6N2E2_542 0.66 -0.5 611,283 - Pf6N2E2_542 0.66 -0.3 611,283 - Pf6N2E2_542 0.66 +0.5 611,504 - Pf6N2E2_542 0.82 +1.7 611,811 + +1.7 611,811 + -0.5 612,215 + -0.6 612,216 - -0.3 612,217 + -0.8 612,217 + -2.0 612,217 + +1.7 612,217 + +0.0 612,217 + +1.7 612,217 + +0.0 612,217 + -0.1 612,217 + +1.0 612,218 - +1.3 612,218 - -1.2 612,218 - -3.0 612,218 - -0.5 612,218 - -1.4 612,218 - -0.3 612,378 - Pf6N2E2_543 0.15 -0.4 612,378 - Pf6N2E2_543 0.15 -0.2 612,648 + Pf6N2E2_543 0.39 +3.3 612,648 + Pf6N2E2_543 0.39 +0.3 612,648 + Pf6N2E2_543 0.39 -1.1 612,648 + Pf6N2E2_543 0.39 +1.7 612,648 + Pf6N2E2_543 0.39 -0.4 612,648 + Pf6N2E2_543 0.39 +0.7 612,648 + Pf6N2E2_543 0.39 +1.0 612,649 - Pf6N2E2_543 0.39 -1.5 612,649 - Pf6N2E2_543 0.39 +1.1 612,649 - Pf6N2E2_543 0.39 -0.6 612,935 + Pf6N2E2_543 0.65 -0.0 612,935 + Pf6N2E2_543 0.65 +0.1 612,936 - Pf6N2E2_543 0.65 +1.7 612,936 - Pf6N2E2_543 0.65 -0.9 612,936 - Pf6N2E2_543 0.65 +0.6 612,941 + Pf6N2E2_543 0.65 -0.4 612,941 + Pf6N2E2_543 0.65 +0.9 612,942 - Pf6N2E2_543 0.65 +0.7 613,031 + Pf6N2E2_543 0.73 -0.2 613,032 - Pf6N2E2_543 0.73 -0.2 613,444 + -1.5 613,444 + -0.3 613,445 - +0.1 613,445 - +1.7 613,445 - -2.4 613,445 - +0.7 613,445 - -1.0 613,445 - -1.4 613,529 + -0.9 613,529 + +0.4 613,553 + -0.2 613,553 + +0.1 613,554 - -0.3 613,769 + Pf6N2E2_544 0.37 -3.1 613,769 + Pf6N2E2_544 0.37 +1.9 613,769 + Pf6N2E2_544 0.37 -1.7 613,770 - Pf6N2E2_544 0.37 +0.3 613,773 + Pf6N2E2_544 0.38 -1.9 613,774 - Pf6N2E2_544 0.38 -1.1 614,266 + +0.2 614,266 + +0.1 614,266 + +0.5 614,266 + -0.5 614,266 + +1.7 614,266 + -2.7 614,266 + -0.5 614,267 - -0.3 614,267 - -1.3 614,267 - +0.7 614,267 - +0.4 614,267 - +0.7
Or see this region's nucleotide sequence