Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2926 and Pf6N2E2_2927 are separated by 277 nucleotides Pf6N2E2_2927 and Pf6N2E2_2928 are separated by 27 nucleotides Pf6N2E2_2928 and Pf6N2E2_2929 overlap by 4 nucleotides Pf6N2E2_2929 and Pf6N2E2_2930 overlap by 4 nucleotides
Pf6N2E2_2926 - ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, ATPase component 2 LivF (from data) , at 3,419,194 to 3,419,895
_2926
Pf6N2E2_2927 - Putative signal peptide protein, at 3,420,173 to 3,420,478
_2927
Pf6N2E2_2928 - Uncharacterized protein conserved in bacteria, NMA0228-like, at 3,420,506 to 3,421,375
_2928
Pf6N2E2_2929 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 3,421,372 to 3,422,130
_2929
Pf6N2E2_2930 - INTEGRAL MEMBRANE PROTEIN (Rhomboid family), at 3,422,127 to 3,422,606
_2930
Position (kb)
3420
3421
3422 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3419.518 kb on + strand, within Pf6N2E2_2926 at 3419.519 kb on - strand, within Pf6N2E2_2926 at 3419.804 kb on - strand, within Pf6N2E2_2926 at 3419.804 kb on - strand, within Pf6N2E2_2926 at 3419.804 kb on - strand, within Pf6N2E2_2926 at 3419.882 kb on - strand at 3420.011 kb on - strand at 3420.091 kb on + strand at 3420.115 kb on + strand at 3420.115 kb on + strand at 3420.116 kb on - strand at 3420.116 kb on - strand at 3420.116 kb on - strand at 3420.116 kb on - strand at 3420.368 kb on + strand, within Pf6N2E2_2927 at 3420.368 kb on + strand, within Pf6N2E2_2927 at 3420.369 kb on - strand, within Pf6N2E2_2927 at 3420.514 kb on + strand at 3420.591 kb on + strand at 3420.592 kb on - strand at 3420.592 kb on - strand at 3420.641 kb on + strand, within Pf6N2E2_2928 at 3420.641 kb on + strand, within Pf6N2E2_2928 at 3420.641 kb on + strand, within Pf6N2E2_2928 at 3420.642 kb on - strand, within Pf6N2E2_2928 at 3420.642 kb on - strand, within Pf6N2E2_2928 at 3420.642 kb on - strand, within Pf6N2E2_2928 at 3420.642 kb on - strand, within Pf6N2E2_2928 at 3420.642 kb on - strand, within Pf6N2E2_2928 at 3420.872 kb on + strand, within Pf6N2E2_2928 at 3420.873 kb on - strand, within Pf6N2E2_2928 at 3420.938 kb on - strand, within Pf6N2E2_2928 at 3420.938 kb on - strand, within Pf6N2E2_2928 at 3420.965 kb on + strand, within Pf6N2E2_2928 at 3420.965 kb on + strand, within Pf6N2E2_2928 at 3420.966 kb on - strand, within Pf6N2E2_2928 at 3420.966 kb on - strand, within Pf6N2E2_2928 at 3421.064 kb on + strand, within Pf6N2E2_2928 at 3421.064 kb on + strand, within Pf6N2E2_2928 at 3421.065 kb on - strand, within Pf6N2E2_2928 at 3421.194 kb on + strand, within Pf6N2E2_2928 at 3421.238 kb on + strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.585 kb on + strand, within Pf6N2E2_2929 at 3421.585 kb on + strand, within Pf6N2E2_2929 at 3421.585 kb on + strand, within Pf6N2E2_2929 at 3421.585 kb on + strand, within Pf6N2E2_2929 at 3421.586 kb on - strand, within Pf6N2E2_2929 at 3421.684 kb on + strand, within Pf6N2E2_2929 at 3421.685 kb on - strand, within Pf6N2E2_2929 at 3421.685 kb on - strand, within Pf6N2E2_2929 at 3422.101 kb on + strand at 3422.101 kb on + strand at 3422.101 kb on + strand at 3422.101 kb on + strand at 3422.102 kb on - strand at 3422.102 kb on - strand at 3422.108 kb on + strand at 3422.108 kb on + strand at 3422.108 kb on + strand at 3422.109 kb on - strand at 3422.121 kb on - strand at 3422.121 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 3,419,518 + Pf6N2E2_2926 0.46 -0.5 3,419,519 - Pf6N2E2_2926 0.46 -0.0 3,419,804 - Pf6N2E2_2926 0.87 +1.0 3,419,804 - Pf6N2E2_2926 0.87 -0.1 3,419,804 - Pf6N2E2_2926 0.87 -0.8 3,419,882 - +0.2 3,420,011 - +0.3 3,420,091 + +0.2 3,420,115 + -0.0 3,420,115 + +0.1 3,420,116 - -0.0 3,420,116 - +0.2 3,420,116 - +0.1 3,420,116 - -1.1 3,420,368 + Pf6N2E2_2927 0.64 +0.1 3,420,368 + Pf6N2E2_2927 0.64 +0.1 3,420,369 - Pf6N2E2_2927 0.64 -0.5 3,420,514 + -0.3 3,420,591 + -0.3 3,420,592 - -1.1 3,420,592 - -1.6 3,420,641 + Pf6N2E2_2928 0.16 -1.1 3,420,641 + Pf6N2E2_2928 0.16 +1.1 3,420,641 + Pf6N2E2_2928 0.16 -1.2 3,420,642 - Pf6N2E2_2928 0.16 -0.5 3,420,642 - Pf6N2E2_2928 0.16 +0.7 3,420,642 - Pf6N2E2_2928 0.16 -0.3 3,420,642 - Pf6N2E2_2928 0.16 -0.3 3,420,642 - Pf6N2E2_2928 0.16 -1.4 3,420,872 + Pf6N2E2_2928 0.42 -0.9 3,420,873 - Pf6N2E2_2928 0.42 +0.2 3,420,938 - Pf6N2E2_2928 0.50 -2.1 3,420,938 - Pf6N2E2_2928 0.50 +0.6 3,420,965 + Pf6N2E2_2928 0.53 +0.7 3,420,965 + Pf6N2E2_2928 0.53 +1.7 3,420,966 - Pf6N2E2_2928 0.53 -1.2 3,420,966 - Pf6N2E2_2928 0.53 -0.5 3,421,064 + Pf6N2E2_2928 0.64 -0.0 3,421,064 + Pf6N2E2_2928 0.64 -0.6 3,421,065 - Pf6N2E2_2928 0.64 +0.7 3,421,194 + Pf6N2E2_2928 0.79 -1.1 3,421,238 + Pf6N2E2_2928 0.84 -0.6 3,421,239 - Pf6N2E2_2928 0.84 -1.1 3,421,239 - Pf6N2E2_2928 0.84 -1.3 3,421,239 - Pf6N2E2_2928 0.84 -1.2 3,421,239 - Pf6N2E2_2928 0.84 -0.2 3,421,239 - Pf6N2E2_2928 0.84 +0.8 3,421,239 - Pf6N2E2_2928 0.84 -0.5 3,421,585 + Pf6N2E2_2929 0.28 -4.0 3,421,585 + Pf6N2E2_2929 0.28 -0.9 3,421,585 + Pf6N2E2_2929 0.28 -0.3 3,421,585 + Pf6N2E2_2929 0.28 -0.2 3,421,586 - Pf6N2E2_2929 0.28 +0.1 3,421,684 + Pf6N2E2_2929 0.41 +0.6 3,421,685 - Pf6N2E2_2929 0.41 -0.7 3,421,685 - Pf6N2E2_2929 0.41 +0.9 3,422,101 + -0.0 3,422,101 + -0.3 3,422,101 + +0.1 3,422,101 + +1.7 3,422,102 - +1.7 3,422,102 - -1.2 3,422,108 + -0.3 3,422,108 + -1.9 3,422,108 + -0.9 3,422,109 - +0.4 3,422,121 - -3.4 3,422,121 - -1.4
Or see this region's nucleotide sequence