Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_263 and Pf6N2E2_264 are separated by 231 nucleotides Pf6N2E2_264 and Pf6N2E2_265 are separated by 145 nucleotides
Pf6N2E2_263 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 326,556 to 327,200
_263
Pf6N2E2_264 - Transcriptional regulator, TetR family, at 327,432 to 327,887
_264
Pf6N2E2_265 - NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3), at 328,033 to 329,496
_265
Position (kb) 327
328 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 326.465 kb on + strand at 326.465 kb on + strand at 326.465 kb on + strand at 326.465 kb on + strand at 326.466 kb on - strand at 326.466 kb on - strand at 326.466 kb on - strand at 326.466 kb on - strand at 326.553 kb on + strand at 326.553 kb on + strand at 326.554 kb on - strand at 326.554 kb on - strand at 326.554 kb on - strand at 326.558 kb on - strand at 326.558 kb on - strand at 326.644 kb on + strand, within Pf6N2E2_263 at 326.644 kb on + strand, within Pf6N2E2_263 at 326.644 kb on + strand, within Pf6N2E2_263 at 326.644 kb on + strand, within Pf6N2E2_263 at 326.645 kb on - strand, within Pf6N2E2_263 at 326.645 kb on - strand, within Pf6N2E2_263 at 326.645 kb on - strand, within Pf6N2E2_263 at 326.645 kb on - strand, within Pf6N2E2_263 at 326.737 kb on + strand, within Pf6N2E2_263 at 326.738 kb on - strand, within Pf6N2E2_263 at 326.738 kb on - strand, within Pf6N2E2_263 at 326.738 kb on - strand, within Pf6N2E2_263 at 326.738 kb on - strand, within Pf6N2E2_263 at 326.784 kb on + strand, within Pf6N2E2_263 at 326.849 kb on + strand, within Pf6N2E2_263 at 326.850 kb on - strand, within Pf6N2E2_263 at 326.980 kb on - strand, within Pf6N2E2_263 at 327.129 kb on + strand, within Pf6N2E2_263 at 327.129 kb on + strand, within Pf6N2E2_263 at 327.130 kb on - strand, within Pf6N2E2_263 at 327.151 kb on - strand at 327.151 kb on - strand at 327.266 kb on + strand at 327.286 kb on + strand at 327.298 kb on - strand at 327.298 kb on - strand at 327.305 kb on + strand at 327.305 kb on + strand at 327.305 kb on + strand at 327.305 kb on + strand at 327.305 kb on + strand at 327.306 kb on - strand at 327.306 kb on - strand at 327.306 kb on - strand at 327.309 kb on + strand at 327.309 kb on + strand at 327.309 kb on + strand at 327.309 kb on + strand at 327.310 kb on - strand at 327.386 kb on - strand at 327.386 kb on - strand at 327.450 kb on + strand at 327.450 kb on + strand at 327.451 kb on - strand at 327.513 kb on + strand, within Pf6N2E2_264 at 327.576 kb on + strand, within Pf6N2E2_264 at 327.577 kb on - strand, within Pf6N2E2_264 at 327.584 kb on + strand, within Pf6N2E2_264 at 327.584 kb on + strand, within Pf6N2E2_264 at 327.748 kb on + strand, within Pf6N2E2_264 at 327.890 kb on + strand at 327.891 kb on - strand at 327.911 kb on + strand at 328.014 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 326,465 + +0.3 326,465 + -0.1 326,465 + -5.0 326,465 + -1.6 326,466 - +1.2 326,466 - -0.7 326,466 - +0.2 326,466 - -0.3 326,553 + -2.5 326,553 + +1.7 326,554 - +0.7 326,554 - -0.3 326,554 - -1.1 326,558 - -3.7 326,558 - -2.5 326,644 + Pf6N2E2_263 0.14 -1.4 326,644 + Pf6N2E2_263 0.14 +0.9 326,644 + Pf6N2E2_263 0.14 -0.4 326,644 + Pf6N2E2_263 0.14 +0.2 326,645 - Pf6N2E2_263 0.14 +0.7 326,645 - Pf6N2E2_263 0.14 +0.2 326,645 - Pf6N2E2_263 0.14 +0.2 326,645 - Pf6N2E2_263 0.14 -1.1 326,737 + Pf6N2E2_263 0.28 +2.5 326,738 - Pf6N2E2_263 0.28 -1.5 326,738 - Pf6N2E2_263 0.28 +1.2 326,738 - Pf6N2E2_263 0.28 -0.0 326,738 - Pf6N2E2_263 0.28 +0.9 326,784 + Pf6N2E2_263 0.35 +1.7 326,849 + Pf6N2E2_263 0.45 -0.0 326,850 - Pf6N2E2_263 0.46 -2.6 326,980 - Pf6N2E2_263 0.66 -0.7 327,129 + Pf6N2E2_263 0.89 -1.7 327,129 + Pf6N2E2_263 0.89 -1.0 327,130 - Pf6N2E2_263 0.89 -1.0 327,151 - +1.1 327,151 - -1.3 327,266 + -0.7 327,286 + -1.7 327,298 - +0.8 327,298 - -0.2 327,305 + -2.4 327,305 + -1.5 327,305 + +0.1 327,305 + -0.2 327,305 + -1.0 327,306 - -0.1 327,306 - +0.6 327,306 - +1.3 327,309 + -1.7 327,309 + +1.8 327,309 + -0.1 327,309 + +0.7 327,310 - -1.9 327,386 - +3.3 327,386 - -0.2 327,450 + +0.8 327,450 + +0.2 327,451 - -2.7 327,513 + Pf6N2E2_264 0.18 +0.8 327,576 + Pf6N2E2_264 0.32 -1.3 327,577 - Pf6N2E2_264 0.32 +1.8 327,584 + Pf6N2E2_264 0.33 -1.6 327,584 + Pf6N2E2_264 0.33 +1.8 327,748 + Pf6N2E2_264 0.69 -1.3 327,890 + -1.1 327,891 - +4.9 327,911 + +0.4 328,014 - -2.2
Or see this region's nucleotide sequence