Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_263

Experiment: nitrogen source nitrite 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_261 and Pf6N2E2_262 are separated by 70 nucleotidesPf6N2E2_262 and Pf6N2E2_263 are separated by 263 nucleotidesPf6N2E2_263 and Pf6N2E2_264 are separated by 231 nucleotidesPf6N2E2_264 and Pf6N2E2_265 are separated by 145 nucleotides Pf6N2E2_261 - Aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 324,816 to 325,925 _261 Pf6N2E2_262 - hypothetical protein, at 325,996 to 326,292 _262 Pf6N2E2_263 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 326,556 to 327,200 _263 Pf6N2E2_264 - Transcriptional regulator, TetR family, at 327,432 to 327,887 _264 Pf6N2E2_265 - NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3), at 328,033 to 329,496 _265 Position (kb) 326 327 328Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4 5at 325.609 kb on - strand, within Pf6N2E2_261at 325.609 kb on - strand, within Pf6N2E2_261at 325.696 kb on + strand, within Pf6N2E2_261at 325.696 kb on + strand, within Pf6N2E2_261at 325.696 kb on + strand, within Pf6N2E2_261at 325.697 kb on - strand, within Pf6N2E2_261at 325.697 kb on - strand, within Pf6N2E2_261at 325.697 kb on - strand, within Pf6N2E2_261at 325.702 kb on + strand, within Pf6N2E2_261at 325.702 kb on + strand, within Pf6N2E2_261at 325.702 kb on + strand, within Pf6N2E2_261at 325.702 kb on + strand, within Pf6N2E2_261at 325.703 kb on - strand, within Pf6N2E2_261at 325.703 kb on - strand, within Pf6N2E2_261at 325.703 kb on - strand, within Pf6N2E2_261at 325.703 kb on - strand, within Pf6N2E2_261at 325.759 kb on + strand, within Pf6N2E2_261at 325.759 kb on + strand, within Pf6N2E2_261at 325.759 kb on + strand, within Pf6N2E2_261at 325.759 kb on + strand, within Pf6N2E2_261at 325.759 kb on + strand, within Pf6N2E2_261at 325.759 kb on + strand, within Pf6N2E2_261at 325.759 kb on + strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.965 kb on + strandat 326.096 kb on + strand, within Pf6N2E2_262at 326.097 kb on - strand, within Pf6N2E2_262at 326.253 kb on - strand, within Pf6N2E2_262at 326.265 kb on + strandat 326.266 kb on - strandat 326.465 kb on + strandat 326.465 kb on + strandat 326.465 kb on + strandat 326.465 kb on + strandat 326.466 kb on - strandat 326.466 kb on - strandat 326.466 kb on - strandat 326.466 kb on - strandat 326.553 kb on + strandat 326.553 kb on + strandat 326.554 kb on - strandat 326.554 kb on - strandat 326.554 kb on - strandat 326.558 kb on - strandat 326.558 kb on - strandat 326.644 kb on + strand, within Pf6N2E2_263at 326.644 kb on + strand, within Pf6N2E2_263at 326.644 kb on + strand, within Pf6N2E2_263at 326.644 kb on + strand, within Pf6N2E2_263at 326.645 kb on - strand, within Pf6N2E2_263at 326.645 kb on - strand, within Pf6N2E2_263at 326.645 kb on - strand, within Pf6N2E2_263at 326.645 kb on - strand, within Pf6N2E2_263at 326.737 kb on + strand, within Pf6N2E2_263at 326.738 kb on - strand, within Pf6N2E2_263at 326.738 kb on - strand, within Pf6N2E2_263at 326.738 kb on - strand, within Pf6N2E2_263at 326.738 kb on - strand, within Pf6N2E2_263at 326.784 kb on + strand, within Pf6N2E2_263at 326.849 kb on + strand, within Pf6N2E2_263at 326.850 kb on - strand, within Pf6N2E2_263at 326.980 kb on - strand, within Pf6N2E2_263at 327.129 kb on + strand, within Pf6N2E2_263at 327.129 kb on + strand, within Pf6N2E2_263at 327.130 kb on - strand, within Pf6N2E2_263at 327.151 kb on - strandat 327.151 kb on - strandat 327.266 kb on + strandat 327.286 kb on + strandat 327.298 kb on - strandat 327.298 kb on - strandat 327.305 kb on + strandat 327.305 kb on + strandat 327.305 kb on + strandat 327.305 kb on + strandat 327.305 kb on + strandat 327.306 kb on - strandat 327.306 kb on - strandat 327.306 kb on - strandat 327.309 kb on + strandat 327.309 kb on + strandat 327.309 kb on + strandat 327.309 kb on + strandat 327.310 kb on - strandat 327.386 kb on - strandat 327.386 kb on - strandat 327.450 kb on + strandat 327.450 kb on + strandat 327.451 kb on - strandat 327.513 kb on + strand, within Pf6N2E2_264at 327.576 kb on + strand, within Pf6N2E2_264at 327.577 kb on - strand, within Pf6N2E2_264at 327.584 kb on + strand, within Pf6N2E2_264at 327.584 kb on + strand, within Pf6N2E2_264at 327.748 kb on + strand, within Pf6N2E2_264at 327.890 kb on + strandat 327.891 kb on - strandat 327.911 kb on + strandat 328.014 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM
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325,609 - Pf6N2E2_261 0.71 -0.6
325,609 - Pf6N2E2_261 0.71 +1.8
325,696 + Pf6N2E2_261 0.79 -0.3
325,696 + Pf6N2E2_261 0.79 -3.5
325,696 + Pf6N2E2_261 0.79 +0.7
325,697 - Pf6N2E2_261 0.79 -1.6
325,697 - Pf6N2E2_261 0.79 +2.5
325,697 - Pf6N2E2_261 0.79 -1.7
325,702 + Pf6N2E2_261 0.80 +0.8
325,702 + Pf6N2E2_261 0.80 -1.8
325,702 + Pf6N2E2_261 0.80 +1.2
325,702 + Pf6N2E2_261 0.80 +0.6
325,703 - Pf6N2E2_261 0.80 -0.3
325,703 - Pf6N2E2_261 0.80 +0.3
325,703 - Pf6N2E2_261 0.80 +1.0
325,703 - Pf6N2E2_261 0.80 -0.8
325,759 + Pf6N2E2_261 0.85 -1.1
325,759 + Pf6N2E2_261 0.85 -0.4
325,759 + Pf6N2E2_261 0.85 -0.7
325,759 + Pf6N2E2_261 0.85 +1.7
325,759 + Pf6N2E2_261 0.85 -0.6
325,759 + Pf6N2E2_261 0.85 -0.1
325,759 + Pf6N2E2_261 0.85 +0.1
325,760 - Pf6N2E2_261 0.85 -0.3
325,760 - Pf6N2E2_261 0.85 +1.7
325,760 - Pf6N2E2_261 0.85 -0.3
325,760 - Pf6N2E2_261 0.85 -0.4
325,760 - Pf6N2E2_261 0.85 +1.2
325,760 - Pf6N2E2_261 0.85 -1.5
325,760 - Pf6N2E2_261 0.85 +0.5
325,760 - Pf6N2E2_261 0.85 -0.7
325,760 - Pf6N2E2_261 0.85 +1.0
325,760 - Pf6N2E2_261 0.85 +0.4
325,760 - Pf6N2E2_261 0.85 +0.7
325,760 - Pf6N2E2_261 0.85 -0.8
325,760 - Pf6N2E2_261 0.85 +1.7
325,965 + +0.7
326,096 + Pf6N2E2_262 0.34 -0.3
326,097 - Pf6N2E2_262 0.34 +0.4
326,253 - Pf6N2E2_262 0.87 +1.7
326,265 + -1.4
326,266 - -1.4
326,465 + +0.3
326,465 + -0.1
326,465 + -5.0
326,465 + -1.6
326,466 - +1.2
326,466 - -0.7
326,466 - +0.2
326,466 - -0.3
326,553 + -2.5
326,553 + +1.7
326,554 - +0.7
326,554 - -0.3
326,554 - -1.1
326,558 - -3.7
326,558 - -2.5
326,644 + Pf6N2E2_263 0.14 -1.4
326,644 + Pf6N2E2_263 0.14 +0.9
326,644 + Pf6N2E2_263 0.14 -0.4
326,644 + Pf6N2E2_263 0.14 +0.2
326,645 - Pf6N2E2_263 0.14 +0.7
326,645 - Pf6N2E2_263 0.14 +0.2
326,645 - Pf6N2E2_263 0.14 +0.2
326,645 - Pf6N2E2_263 0.14 -1.1
326,737 + Pf6N2E2_263 0.28 +2.5
326,738 - Pf6N2E2_263 0.28 -1.5
326,738 - Pf6N2E2_263 0.28 +1.2
326,738 - Pf6N2E2_263 0.28 -0.0
326,738 - Pf6N2E2_263 0.28 +0.9
326,784 + Pf6N2E2_263 0.35 +1.7
326,849 + Pf6N2E2_263 0.45 -0.0
326,850 - Pf6N2E2_263 0.46 -2.6
326,980 - Pf6N2E2_263 0.66 -0.7
327,129 + Pf6N2E2_263 0.89 -1.7
327,129 + Pf6N2E2_263 0.89 -1.0
327,130 - Pf6N2E2_263 0.89 -1.0
327,151 - +1.1
327,151 - -1.3
327,266 + -0.7
327,286 + -1.7
327,298 - +0.8
327,298 - -0.2
327,305 + -2.4
327,305 + -1.5
327,305 + +0.1
327,305 + -0.2
327,305 + -1.0
327,306 - -0.1
327,306 - +0.6
327,306 - +1.3
327,309 + -1.7
327,309 + +1.8
327,309 + -0.1
327,309 + +0.7
327,310 - -1.9
327,386 - +3.3
327,386 - -0.2
327,450 + +0.8
327,450 + +0.2
327,451 - -2.7
327,513 + Pf6N2E2_264 0.18 +0.8
327,576 + Pf6N2E2_264 0.32 -1.3
327,577 - Pf6N2E2_264 0.32 +1.8
327,584 + Pf6N2E2_264 0.33 -1.6
327,584 + Pf6N2E2_264 0.33 +1.8
327,748 + Pf6N2E2_264 0.69 -1.3
327,890 + -1.1
327,891 - +4.9
327,911 + +0.4
328,014 - -2.2

Or see this region's nucleotide sequence