Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1868 and Pf6N2E2_1869 are separated by 822 nucleotides Pf6N2E2_1869 and Pf6N2E2_1870 are separated by 788 nucleotides
Pf6N2E2_1868 - hypothetical protein, at 2,213,819 to 2,214,892
_1868
Pf6N2E2_1869 - hypothetical protein, at 2,215,715 to 2,215,939
_1869
Pf6N2E2_1870 - hypothetical protein, at 2,216,728 to 2,217,258
_1870
Position (kb)
2215
2216 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2214.792 kb on + strand at 2214.793 kb on - strand at 2214.824 kb on + strand at 2214.927 kb on - strand at 2214.927 kb on - strand at 2214.980 kb on + strand at 2215.006 kb on - strand at 2215.012 kb on + strand at 2215.012 kb on + strand at 2215.012 kb on + strand at 2215.145 kb on + strand at 2215.146 kb on - strand at 2215.168 kb on + strand at 2215.169 kb on - strand at 2215.169 kb on - strand at 2215.169 kb on - strand at 2215.221 kb on + strand at 2215.240 kb on + strand at 2215.361 kb on + strand at 2215.388 kb on + strand at 2215.388 kb on + strand at 2215.388 kb on + strand at 2215.389 kb on - strand at 2215.394 kb on + strand at 2215.616 kb on + strand at 2215.616 kb on + strand at 2215.616 kb on + strand at 2215.616 kb on + strand at 2215.649 kb on + strand at 2215.650 kb on - strand at 2215.690 kb on - strand at 2215.788 kb on + strand, within Pf6N2E2_1869 at 2215.788 kb on + strand, within Pf6N2E2_1869 at 2215.789 kb on - strand, within Pf6N2E2_1869 at 2215.789 kb on - strand, within Pf6N2E2_1869 at 2215.824 kb on - strand, within Pf6N2E2_1869 at 2215.875 kb on + strand, within Pf6N2E2_1869 at 2215.875 kb on + strand, within Pf6N2E2_1869 at 2215.876 kb on - strand, within Pf6N2E2_1869 at 2215.876 kb on - strand, within Pf6N2E2_1869 at 2215.907 kb on + strand, within Pf6N2E2_1869 at 2215.916 kb on + strand, within Pf6N2E2_1869 at 2215.952 kb on + strand at 2216.002 kb on + strand at 2216.032 kb on + strand at 2216.032 kb on + strand at 2216.032 kb on + strand at 2216.044 kb on - strand at 2216.044 kb on - strand at 2216.086 kb on + strand at 2216.429 kb on + strand at 2216.430 kb on - strand at 2216.469 kb on + strand at 2216.470 kb on - strand at 2216.578 kb on + strand at 2216.578 kb on + strand at 2216.579 kb on - strand at 2216.579 kb on - strand at 2216.610 kb on - strand at 2216.683 kb on + strand at 2216.684 kb on - strand at 2216.684 kb on - strand at 2216.739 kb on + strand at 2216.740 kb on - strand at 2216.740 kb on - strand at 2216.740 kb on - strand at 2216.750 kb on - strand at 2216.750 kb on - strand at 2216.938 kb on + strand, within Pf6N2E2_1870
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 2,214,792 + +1.7 2,214,793 - +1.7 2,214,824 + +1.7 2,214,927 - -0.8 2,214,927 - +1.4 2,214,980 + +0.6 2,215,006 - +0.1 2,215,012 + -2.5 2,215,012 + -0.8 2,215,012 + +1.7 2,215,145 + +1.3 2,215,146 - -0.5 2,215,168 + -2.4 2,215,169 - +3.3 2,215,169 - +2.7 2,215,169 - +0.4 2,215,221 + -1.9 2,215,240 + -0.3 2,215,361 + -0.0 2,215,388 + +0.1 2,215,388 + +0.7 2,215,388 + +1.7 2,215,389 - +1.7 2,215,394 + +1.7 2,215,616 + +1.7 2,215,616 + -0.3 2,215,616 + +0.7 2,215,616 + -0.5 2,215,649 + -0.3 2,215,650 - +0.1 2,215,690 - +1.4 2,215,788 + Pf6N2E2_1869 0.32 +0.7 2,215,788 + Pf6N2E2_1869 0.32 -2.3 2,215,789 - Pf6N2E2_1869 0.33 +0.7 2,215,789 - Pf6N2E2_1869 0.33 -0.9 2,215,824 - Pf6N2E2_1869 0.48 -1.0 2,215,875 + Pf6N2E2_1869 0.71 +1.7 2,215,875 + Pf6N2E2_1869 0.71 -1.9 2,215,876 - Pf6N2E2_1869 0.72 -1.5 2,215,876 - Pf6N2E2_1869 0.72 -0.3 2,215,907 + Pf6N2E2_1869 0.85 +1.7 2,215,916 + Pf6N2E2_1869 0.89 -0.3 2,215,952 + -0.5 2,216,002 + +0.5 2,216,032 + -1.1 2,216,032 + +1.7 2,216,032 + +0.2 2,216,044 - -1.7 2,216,044 - -1.7 2,216,086 + -0.3 2,216,429 + +1.7 2,216,430 - +1.7 2,216,469 + -1.1 2,216,470 - +1.7 2,216,578 + -0.2 2,216,578 + -1.5 2,216,579 - -0.6 2,216,579 - -0.3 2,216,610 - +0.3 2,216,683 + -1.9 2,216,684 - +1.3 2,216,684 - -0.4 2,216,739 + -0.4 2,216,740 - -0.9 2,216,740 - +1.1 2,216,740 - +0.4 2,216,750 - -2.8 2,216,750 - -0.7 2,216,938 + Pf6N2E2_1870 0.40 -1.5
Or see this region's nucleotide sequence