Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1670 and Pf6N2E2_1671 are separated by 24 nucleotides Pf6N2E2_1671 and Pf6N2E2_1672 are separated by 148 nucleotides Pf6N2E2_1672 and Pf6N2E2_1673 are separated by 19 nucleotides
Pf6N2E2_1670 - conserved membrane protein, at 1,976,801 to 1,977,310
_1670
Pf6N2E2_1671 - Transcriptional regulator, TetR family, at 1,977,335 to 1,978,012
_1671
Pf6N2E2_1672 - hypothetical protein, at 1,978,161 to 1,979,042
_1672
Pf6N2E2_1673 - Transcriptional regulator, TetR family, at 1,979,062 to 1,979,499
_1673
Position (kb)
1978
1979
1980 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1977.182 kb on + strand, within Pf6N2E2_1670 at 1977.183 kb on - strand, within Pf6N2E2_1670 at 1977.211 kb on - strand, within Pf6N2E2_1670 at 1977.258 kb on - strand, within Pf6N2E2_1670 at 1977.284 kb on + strand at 1977.284 kb on + strand at 1977.390 kb on + strand at 1977.390 kb on + strand at 1977.391 kb on - strand at 1977.391 kb on - strand at 1977.391 kb on - strand at 1977.391 kb on - strand at 1977.471 kb on - strand at 1977.471 kb on - strand, within Pf6N2E2_1671 at 1977.471 kb on - strand, within Pf6N2E2_1671 at 1977.472 kb on + strand, within Pf6N2E2_1671 at 1977.605 kb on + strand, within Pf6N2E2_1671 at 1977.716 kb on + strand, within Pf6N2E2_1671 at 1977.716 kb on + strand, within Pf6N2E2_1671 at 1977.717 kb on - strand, within Pf6N2E2_1671 at 1977.828 kb on - strand, within Pf6N2E2_1671 at 1977.837 kb on + strand, within Pf6N2E2_1671 at 1978.027 kb on - strand at 1978.029 kb on - strand at 1978.029 kb on - strand at 1978.029 kb on - strand at 1978.035 kb on - strand at 1978.063 kb on + strand at 1978.063 kb on + strand at 1978.067 kb on + strand at 1978.067 kb on + strand at 1978.068 kb on - strand at 1978.068 kb on - strand at 1978.092 kb on + strand at 1978.093 kb on - strand at 1978.143 kb on + strand at 1978.143 kb on + strand at 1978.144 kb on - strand at 1978.144 kb on - strand at 1978.144 kb on - strand at 1978.154 kb on + strand at 1978.155 kb on - strand at 1978.155 kb on - strand at 1978.174 kb on + strand at 1978.191 kb on + strand at 1978.254 kb on + strand, within Pf6N2E2_1672 at 1978.408 kb on - strand, within Pf6N2E2_1672 at 1978.435 kb on - strand, within Pf6N2E2_1672 at 1978.435 kb on - strand, within Pf6N2E2_1672 at 1978.435 kb on - strand, within Pf6N2E2_1672 at 1978.435 kb on - strand, within Pf6N2E2_1672 at 1978.521 kb on + strand, within Pf6N2E2_1672 at 1978.540 kb on - strand, within Pf6N2E2_1672 at 1978.614 kb on + strand, within Pf6N2E2_1672 at 1978.615 kb on - strand, within Pf6N2E2_1672 at 1978.615 kb on - strand, within Pf6N2E2_1672 at 1978.615 kb on - strand, within Pf6N2E2_1672 at 1978.615 kb on - strand, within Pf6N2E2_1672 at 1978.622 kb on + strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.623 kb on - strand, within Pf6N2E2_1672 at 1978.642 kb on - strand, within Pf6N2E2_1672 at 1978.642 kb on - strand, within Pf6N2E2_1672 at 1978.732 kb on + strand, within Pf6N2E2_1672 at 1978.733 kb on - strand, within Pf6N2E2_1672 at 1978.780 kb on + strand, within Pf6N2E2_1672 at 1978.780 kb on + strand, within Pf6N2E2_1672 at 1978.780 kb on + strand, within Pf6N2E2_1672 at 1978.830 kb on + strand, within Pf6N2E2_1672 at 1978.831 kb on - strand, within Pf6N2E2_1672 at 1978.906 kb on + strand, within Pf6N2E2_1672 at 1978.907 kb on - strand, within Pf6N2E2_1672 at 1979.040 kb on + strand at 1979.040 kb on + strand at 1979.227 kb on - strand, within Pf6N2E2_1673 at 1979.227 kb on - strand, within Pf6N2E2_1673 at 1979.446 kb on - strand, within Pf6N2E2_1673 at 1979.483 kb on + strand at 1979.484 kb on - strand at 1979.525 kb on + strand at 1979.526 kb on - strand at 1979.598 kb on + strand at 1979.599 kb on - strand at 1979.599 kb on - strand at 1979.599 kb on - strand at 1979.599 kb on - strand at 1979.608 kb on + strand at 1979.609 kb on - strand at 1979.688 kb on + strand at 1979.689 kb on - strand at 1979.778 kb on + strand at 1979.781 kb on - strand at 1979.781 kb on - strand at 1979.785 kb on - strand at 1979.785 kb on - strand at 1979.785 kb on - strand at 1979.787 kb on - strand at 1979.787 kb on - strand at 1979.828 kb on + strand at 1979.829 kb on - strand at 1979.829 kb on - strand at 1979.829 kb on - strand at 1979.842 kb on - strand at 1979.849 kb on + strand at 1979.849 kb on + strand at 1979.850 kb on - strand at 1979.910 kb on + strand at 1979.910 kb on + strand at 1979.914 kb on + strand at 1979.937 kb on + strand at 1979.986 kb on + strand at 1980.008 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 1,977,182 + Pf6N2E2_1670 0.75 +0.4 1,977,183 - Pf6N2E2_1670 0.75 +1.8 1,977,211 - Pf6N2E2_1670 0.80 -1.3 1,977,258 - Pf6N2E2_1670 0.90 +0.7 1,977,284 + -0.7 1,977,284 + -0.8 1,977,390 + -1.5 1,977,390 + -0.9 1,977,391 - -1.3 1,977,391 - +0.3 1,977,391 - +0.3 1,977,391 - +0.7 1,977,471 - -0.3 1,977,471 - Pf6N2E2_1671 0.20 -1.5 1,977,471 - Pf6N2E2_1671 0.20 +1.0 1,977,472 + Pf6N2E2_1671 0.20 +0.5 1,977,605 + Pf6N2E2_1671 0.40 -0.6 1,977,716 + Pf6N2E2_1671 0.56 +1.7 1,977,716 + Pf6N2E2_1671 0.56 +1.7 1,977,717 - Pf6N2E2_1671 0.56 +0.1 1,977,828 - Pf6N2E2_1671 0.73 -0.6 1,977,837 + Pf6N2E2_1671 0.74 -0.5 1,978,027 - -3.2 1,978,029 - +1.7 1,978,029 - +0.7 1,978,029 - +1.7 1,978,035 - +0.7 1,978,063 + -1.1 1,978,063 + -1.6 1,978,067 + -1.7 1,978,067 + -0.5 1,978,068 - -2.4 1,978,068 - +1.7 1,978,092 + -1.9 1,978,093 - +0.1 1,978,143 + -2.6 1,978,143 + +1.7 1,978,144 - +0.7 1,978,144 - -1.9 1,978,144 - -1.9 1,978,154 + -1.9 1,978,155 - -0.6 1,978,155 - +0.7 1,978,174 + +2.7 1,978,191 + -2.3 1,978,254 + Pf6N2E2_1672 0.11 -1.8 1,978,408 - Pf6N2E2_1672 0.28 -0.6 1,978,435 - Pf6N2E2_1672 0.31 -4.7 1,978,435 - Pf6N2E2_1672 0.31 +0.7 1,978,435 - Pf6N2E2_1672 0.31 +1.7 1,978,435 - Pf6N2E2_1672 0.31 -2.1 1,978,521 + Pf6N2E2_1672 0.41 -1.4 1,978,540 - Pf6N2E2_1672 0.43 -4.7 1,978,614 + Pf6N2E2_1672 0.51 -1.5 1,978,615 - Pf6N2E2_1672 0.51 -0.9 1,978,615 - Pf6N2E2_1672 0.51 +0.3 1,978,615 - Pf6N2E2_1672 0.51 -2.1 1,978,615 - Pf6N2E2_1672 0.51 +0.1 1,978,622 + Pf6N2E2_1672 0.52 -3.4 1,978,623 - Pf6N2E2_1672 0.52 +0.5 1,978,623 - Pf6N2E2_1672 0.52 +0.1 1,978,623 - Pf6N2E2_1672 0.52 -2.9 1,978,623 - Pf6N2E2_1672 0.52 -1.2 1,978,623 - Pf6N2E2_1672 0.52 -2.4 1,978,623 - Pf6N2E2_1672 0.52 -2.8 1,978,642 - Pf6N2E2_1672 0.55 +1.7 1,978,642 - Pf6N2E2_1672 0.55 +0.1 1,978,732 + Pf6N2E2_1672 0.65 -0.5 1,978,733 - Pf6N2E2_1672 0.65 +0.7 1,978,780 + Pf6N2E2_1672 0.70 +0.7 1,978,780 + Pf6N2E2_1672 0.70 -0.4 1,978,780 + Pf6N2E2_1672 0.70 -1.6 1,978,830 + Pf6N2E2_1672 0.76 -2.5 1,978,831 - Pf6N2E2_1672 0.76 -3.6 1,978,906 + Pf6N2E2_1672 0.84 -1.6 1,978,907 - Pf6N2E2_1672 0.85 +0.7 1,979,040 + -2.2 1,979,040 + -1.5 1,979,227 - Pf6N2E2_1673 0.38 -0.9 1,979,227 - Pf6N2E2_1673 0.38 +1.7 1,979,446 - Pf6N2E2_1673 0.88 -1.4 1,979,483 + -1.7 1,979,484 - -0.1 1,979,525 + -2.1 1,979,526 - +0.2 1,979,598 + -1.1 1,979,599 - -0.8 1,979,599 - +0.6 1,979,599 - +0.9 1,979,599 - -0.3 1,979,608 + +0.7 1,979,609 - +1.2 1,979,688 + -1.6 1,979,689 - +0.7 1,979,778 + +0.7 1,979,781 - +0.3 1,979,781 - +2.2 1,979,785 - +1.0 1,979,785 - +0.7 1,979,785 - +0.8 1,979,787 - +0.7 1,979,787 - -0.6 1,979,828 + +1.9 1,979,829 - +1.7 1,979,829 - +0.4 1,979,829 - +1.7 1,979,842 - +1.4 1,979,849 + +1.5 1,979,849 + +1.1 1,979,850 - +1.0 1,979,910 + +0.4 1,979,910 + +2.6 1,979,914 + +0.1 1,979,937 + +1.7 1,979,986 + +0.1 1,980,008 + +0.9
Or see this region's nucleotide sequence