Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1016 and Pf6N2E2_1017 overlap by 4 nucleotides Pf6N2E2_1017 and Pf6N2E2_1018 are separated by 124 nucleotides
Pf6N2E2_1016 - Protein involved in biosynthesis of mitomycin antibiotics/polyketide fumonisin, at 1,185,906 to 1,187,060
_1016
Pf6N2E2_1017 - small molecule metabolism, at 1,187,057 to 1,187,905
_1017
Pf6N2E2_1018 - putative DNA-binding protein, at 1,188,030 to 1,189,055
_1018
Position (kb)
1188
1189
1190 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1187.430 kb on + strand, within Pf6N2E2_1017 at 1187.865 kb on + strand at 1187.865 kb on + strand at 1187.885 kb on - strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.922 kb on - strand at 1187.968 kb on + strand at 1187.969 kb on - strand at 1188.112 kb on + strand at 1188.113 kb on - strand at 1188.113 kb on - strand at 1188.135 kb on + strand, within Pf6N2E2_1018 at 1188.212 kb on + strand, within Pf6N2E2_1018 at 1188.212 kb on + strand, within Pf6N2E2_1018 at 1188.213 kb on - strand, within Pf6N2E2_1018 at 1188.213 kb on - strand, within Pf6N2E2_1018 at 1188.213 kb on - strand, within Pf6N2E2_1018 at 1188.404 kb on - strand, within Pf6N2E2_1018 at 1188.404 kb on - strand, within Pf6N2E2_1018 at 1188.404 kb on - strand, within Pf6N2E2_1018 at 1188.404 kb on - strand, within Pf6N2E2_1018 at 1188.406 kb on + strand, within Pf6N2E2_1018 at 1188.407 kb on - strand, within Pf6N2E2_1018 at 1188.407 kb on - strand, within Pf6N2E2_1018 at 1188.407 kb on - strand, within Pf6N2E2_1018 at 1188.407 kb on - strand, within Pf6N2E2_1018 at 1188.407 kb on - strand, within Pf6N2E2_1018 at 1188.434 kb on - strand, within Pf6N2E2_1018 at 1188.519 kb on + strand, within Pf6N2E2_1018 at 1188.520 kb on - strand, within Pf6N2E2_1018 at 1188.561 kb on + strand, within Pf6N2E2_1018 at 1188.561 kb on + strand, within Pf6N2E2_1018 at 1188.789 kb on + strand, within Pf6N2E2_1018 at 1188.789 kb on + strand, within Pf6N2E2_1018 at 1188.790 kb on - strand, within Pf6N2E2_1018 at 1188.968 kb on + strand at 1189.008 kb on - strand at 1189.008 kb on - strand at 1189.050 kb on + strand at 1189.131 kb on - strand at 1189.131 kb on - strand at 1189.167 kb on - strand at 1189.172 kb on + strand at 1189.225 kb on - strand at 1189.228 kb on + strand at 1189.229 kb on - strand at 1189.246 kb on - strand at 1189.253 kb on + strand at 1189.253 kb on + strand at 1189.254 kb on - strand at 1189.304 kb on - strand at 1189.382 kb on + strand at 1189.405 kb on + strand at 1189.418 kb on + strand at 1189.419 kb on - strand at 1189.419 kb on - strand at 1189.422 kb on + strand at 1189.422 kb on + strand at 1189.422 kb on + strand at 1189.422 kb on + strand at 1189.422 kb on + strand at 1189.423 kb on - strand at 1189.428 kb on + strand at 1189.428 kb on + strand at 1189.428 kb on + strand at 1189.428 kb on + strand at 1189.429 kb on - strand at 1189.478 kb on - strand at 1189.560 kb on + strand at 1189.639 kb on + strand at 1189.639 kb on + strand at 1189.639 kb on + strand at 1189.639 kb on + strand at 1189.639 kb on + strand at 1189.639 kb on + strand at 1189.640 kb on - strand at 1189.640 kb on - strand at 1189.640 kb on - strand at 1189.723 kb on + strand at 1189.806 kb on + strand at 1189.806 kb on + strand at 1189.907 kb on + strand at 1189.907 kb on + strand at 1189.907 kb on + strand at 1189.907 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 1,187,430 + Pf6N2E2_1017 0.44 +0.4 1,187,865 + +0.8 1,187,865 + +1.8 1,187,885 - +0.9 1,187,921 + -0.1 1,187,921 + -1.5 1,187,921 + -0.8 1,187,921 + +1.8 1,187,921 + +1.8 1,187,921 + -2.7 1,187,921 + -0.2 1,187,921 + -0.9 1,187,922 - +0.2 1,187,968 + -3.8 1,187,969 - +0.5 1,188,112 + +0.0 1,188,113 - -0.4 1,188,113 - +1.3 1,188,135 + Pf6N2E2_1018 0.10 -1.2 1,188,212 + Pf6N2E2_1018 0.18 -0.8 1,188,212 + Pf6N2E2_1018 0.18 +0.8 1,188,213 - Pf6N2E2_1018 0.18 -1.2 1,188,213 - Pf6N2E2_1018 0.18 -0.5 1,188,213 - Pf6N2E2_1018 0.18 -2.0 1,188,404 - Pf6N2E2_1018 0.36 +0.1 1,188,404 - Pf6N2E2_1018 0.36 +0.6 1,188,404 - Pf6N2E2_1018 0.36 +1.1 1,188,404 - Pf6N2E2_1018 0.36 -0.7 1,188,406 + Pf6N2E2_1018 0.37 +1.8 1,188,407 - Pf6N2E2_1018 0.37 -0.5 1,188,407 - Pf6N2E2_1018 0.37 -0.1 1,188,407 - Pf6N2E2_1018 0.37 -0.6 1,188,407 - Pf6N2E2_1018 0.37 -0.6 1,188,407 - Pf6N2E2_1018 0.37 -1.2 1,188,434 - Pf6N2E2_1018 0.39 -0.6 1,188,519 + Pf6N2E2_1018 0.48 -1.8 1,188,520 - Pf6N2E2_1018 0.48 -0.4 1,188,561 + Pf6N2E2_1018 0.52 -1.9 1,188,561 + Pf6N2E2_1018 0.52 +0.4 1,188,789 + Pf6N2E2_1018 0.74 -0.1 1,188,789 + Pf6N2E2_1018 0.74 -1.9 1,188,790 - Pf6N2E2_1018 0.74 -0.2 1,188,968 + +0.8 1,189,008 - +1.8 1,189,008 - -1.4 1,189,050 + -0.6 1,189,131 - +1.8 1,189,131 - +0.2 1,189,167 - -2.2 1,189,172 + +0.6 1,189,225 - -2.1 1,189,228 + +0.5 1,189,229 - -1.0 1,189,246 - +0.2 1,189,253 + +0.2 1,189,253 + +1.1 1,189,254 - +1.1 1,189,304 - -0.3 1,189,382 + +1.8 1,189,405 + -0.5 1,189,418 + +0.8 1,189,419 - -1.9 1,189,419 - -0.5 1,189,422 + +1.0 1,189,422 + +0.2 1,189,422 + -0.0 1,189,422 + +0.2 1,189,422 + +1.8 1,189,423 - +1.8 1,189,428 + -1.2 1,189,428 + +0.9 1,189,428 + +0.8 1,189,428 + -1.9 1,189,429 - +0.2 1,189,478 - -2.6 1,189,560 + -0.9 1,189,639 + -0.3 1,189,639 + +1.8 1,189,639 + +0.2 1,189,639 + -2.2 1,189,639 + -0.7 1,189,639 + +0.1 1,189,640 - +0.8 1,189,640 - +0.2 1,189,640 - +1.3 1,189,723 + -0.1 1,189,806 + +1.5 1,189,806 + +1.8 1,189,907 + +1.0 1,189,907 + +0.8 1,189,907 + -1.1 1,189,907 + -0.2
Or see this region's nucleotide sequence