Experiment: nitrogen source nitrite 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5909 and Pf6N2E2_5910 are separated by 78 nucleotides Pf6N2E2_5910 and Pf6N2E2_5911 are separated by 25 nucleotides Pf6N2E2_5911 and Pf6N2E2_5912 are separated by 8 nucleotides Pf6N2E2_5912 and Pf6N2E2_5913 overlap by 1 nucleotides Pf6N2E2_5913 and Pf6N2E2_5914 overlap by 7 nucleotides
Pf6N2E2_5909 - Predicted ATPase with chaperone activity, associated with Flp pilus assembly, at 6,727,567 to 6,728,895
_5909
Pf6N2E2_5910 - Flp pilus assembly protein RcpC/CpaB, at 6,728,974 to 6,729,723
_5910
Pf6N2E2_5911 - Von Willebrand factor type A domain protein, associated with Flp pilus assembly, at 6,729,749 to 6,731,110
_5911
Pf6N2E2_5912 - Similar to TadZ/CpaE, associated with Flp pilus assembly, at 6,731,119 to 6,731,598
_5912
Pf6N2E2_5913 - Similar to TadZ/CpaE, associated with Flp pilus assembly, at 6,731,598 to 6,732,092
_5913
Pf6N2E2_5914 - Type II/IV secretion system ATPase TadZ/CpaE, associated with Flp pilus assembly, at 6,732,086 to 6,733,306
_5914
Position (kb)
6729
6730
6731
6732 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6728.812 kb on + strand at 6728.869 kb on + strand at 6728.869 kb on + strand at 6728.869 kb on + strand at 6728.869 kb on + strand at 6728.869 kb on + strand at 6728.869 kb on + strand at 6728.870 kb on - strand at 6728.870 kb on - strand at 6728.870 kb on - strand at 6728.870 kb on - strand at 6728.922 kb on + strand at 6728.922 kb on + strand at 6728.923 kb on - strand at 6729.156 kb on - strand, within Pf6N2E2_5910 at 6729.644 kb on + strand, within Pf6N2E2_5910 at 6729.645 kb on - strand, within Pf6N2E2_5910 at 6729.782 kb on + strand at 6729.782 kb on + strand at 6729.782 kb on + strand at 6729.782 kb on + strand at 6729.783 kb on - strand at 6729.837 kb on + strand at 6729.837 kb on + strand at 6729.837 kb on + strand at 6729.837 kb on + strand at 6729.837 kb on + strand at 6729.837 kb on + strand at 6729.838 kb on - strand at 6729.838 kb on - strand at 6730.131 kb on - strand, within Pf6N2E2_5911 at 6730.145 kb on + strand, within Pf6N2E2_5911 at 6730.145 kb on + strand, within Pf6N2E2_5911 at 6730.146 kb on - strand, within Pf6N2E2_5911 at 6730.146 kb on - strand, within Pf6N2E2_5911 at 6730.169 kb on + strand, within Pf6N2E2_5911 at 6730.169 kb on + strand, within Pf6N2E2_5911 at 6730.170 kb on - strand, within Pf6N2E2_5911 at 6730.170 kb on - strand, within Pf6N2E2_5911 at 6730.170 kb on - strand, within Pf6N2E2_5911 at 6730.170 kb on - strand, within Pf6N2E2_5911 at 6730.170 kb on - strand, within Pf6N2E2_5911 at 6730.173 kb on + strand, within Pf6N2E2_5911 at 6730.174 kb on - strand, within Pf6N2E2_5911 at 6730.193 kb on + strand, within Pf6N2E2_5911 at 6730.193 kb on + strand, within Pf6N2E2_5911 at 6730.193 kb on + strand, within Pf6N2E2_5911 at 6730.552 kb on + strand, within Pf6N2E2_5911 at 6730.552 kb on + strand, within Pf6N2E2_5911 at 6730.552 kb on + strand, within Pf6N2E2_5911 at 6730.553 kb on - strand, within Pf6N2E2_5911 at 6730.553 kb on - strand, within Pf6N2E2_5911 at 6730.555 kb on + strand, within Pf6N2E2_5911 at 6730.567 kb on + strand, within Pf6N2E2_5911 at 6730.567 kb on + strand, within Pf6N2E2_5911 at 6730.585 kb on + strand, within Pf6N2E2_5911 at 6730.601 kb on + strand, within Pf6N2E2_5911 at 6730.601 kb on + strand, within Pf6N2E2_5911 at 6730.601 kb on + strand, within Pf6N2E2_5911 at 6730.601 kb on + strand, within Pf6N2E2_5911 at 6730.601 kb on + strand, within Pf6N2E2_5911 at 6730.602 kb on - strand, within Pf6N2E2_5911 at 6730.602 kb on - strand, within Pf6N2E2_5911 at 6730.602 kb on - strand, within Pf6N2E2_5911 at 6730.673 kb on + strand, within Pf6N2E2_5911 at 6730.674 kb on - strand, within Pf6N2E2_5911 at 6730.674 kb on - strand, within Pf6N2E2_5911 at 6730.700 kb on + strand, within Pf6N2E2_5911 at 6730.700 kb on + strand, within Pf6N2E2_5911 at 6730.701 kb on - strand, within Pf6N2E2_5911 at 6730.766 kb on + strand, within Pf6N2E2_5911 at 6730.766 kb on + strand, within Pf6N2E2_5911 at 6730.766 kb on + strand, within Pf6N2E2_5911 at 6730.766 kb on + strand, within Pf6N2E2_5911 at 6730.767 kb on - strand, within Pf6N2E2_5911 at 6730.928 kb on + strand, within Pf6N2E2_5911 at 6730.929 kb on - strand, within Pf6N2E2_5911 at 6730.972 kb on + strand, within Pf6N2E2_5911 at 6730.973 kb on - strand, within Pf6N2E2_5911 at 6731.013 kb on + strand at 6731.013 kb on + strand at 6731.013 kb on + strand at 6731.024 kb on - strand at 6731.063 kb on + strand at 6731.072 kb on + strand at 6731.073 kb on - strand at 6731.108 kb on + strand at 6731.109 kb on - strand at 6731.109 kb on - strand at 6731.242 kb on + strand, within Pf6N2E2_5912 at 6731.248 kb on + strand, within Pf6N2E2_5912 at 6731.248 kb on + strand, within Pf6N2E2_5912 at 6731.248 kb on + strand, within Pf6N2E2_5912 at 6731.248 kb on + strand, within Pf6N2E2_5912 at 6731.249 kb on - strand, within Pf6N2E2_5912 at 6731.249 kb on - strand, within Pf6N2E2_5912 at 6731.249 kb on - strand, within Pf6N2E2_5912 at 6731.249 kb on - strand, within Pf6N2E2_5912 at 6731.258 kb on + strand, within Pf6N2E2_5912 at 6731.258 kb on + strand, within Pf6N2E2_5912 at 6731.259 kb on - strand, within Pf6N2E2_5912 at 6731.310 kb on + strand, within Pf6N2E2_5912 at 6731.310 kb on + strand, within Pf6N2E2_5912 at 6731.311 kb on - strand, within Pf6N2E2_5912 at 6731.311 kb on - strand, within Pf6N2E2_5912 at 6731.374 kb on + strand, within Pf6N2E2_5912 at 6731.374 kb on + strand, within Pf6N2E2_5912 at 6731.375 kb on - strand, within Pf6N2E2_5912 at 6731.375 kb on - strand, within Pf6N2E2_5912 at 6731.479 kb on + strand, within Pf6N2E2_5912 at 6731.479 kb on + strand, within Pf6N2E2_5912 at 6731.480 kb on - strand, within Pf6N2E2_5912 at 6731.480 kb on - strand, within Pf6N2E2_5912 at 6731.480 kb on - strand, within Pf6N2E2_5912 at 6731.508 kb on + strand, within Pf6N2E2_5912 at 6731.625 kb on + strand at 6731.625 kb on + strand at 6731.626 kb on - strand at 6731.626 kb on - strand at 6731.626 kb on - strand at 6731.700 kb on + strand, within Pf6N2E2_5913 at 6731.700 kb on + strand, within Pf6N2E2_5913 at 6731.700 kb on + strand, within Pf6N2E2_5913 at 6731.701 kb on - strand, within Pf6N2E2_5913 at 6731.701 kb on - strand, within Pf6N2E2_5913 at 6731.701 kb on - strand, within Pf6N2E2_5913 at 6731.765 kb on + strand, within Pf6N2E2_5913 at 6731.766 kb on - strand, within Pf6N2E2_5913 at 6731.871 kb on + strand, within Pf6N2E2_5913 at 6731.872 kb on - strand, within Pf6N2E2_5913 at 6731.872 kb on - strand, within Pf6N2E2_5913 at 6731.947 kb on - strand, within Pf6N2E2_5913 at 6732.011 kb on + strand, within Pf6N2E2_5913 at 6732.012 kb on - strand, within Pf6N2E2_5913 at 6732.095 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM remove 6,728,812 + -0.0 6,728,869 + -1.9 6,728,869 + +1.5 6,728,869 + +0.5 6,728,869 + +1.5 6,728,869 + +1.5 6,728,869 + -0.2 6,728,870 - +1.5 6,728,870 - -0.6 6,728,870 - +0.5 6,728,870 - -0.5 6,728,922 + +0.4 6,728,922 + +1.5 6,728,923 - -0.1 6,729,156 - Pf6N2E2_5910 0.24 -1.9 6,729,644 + Pf6N2E2_5910 0.89 -0.5 6,729,645 - Pf6N2E2_5910 0.89 -0.1 6,729,782 + +0.6 6,729,782 + -0.1 6,729,782 + -1.2 6,729,782 + -1.1 6,729,783 - +1.5 6,729,837 + +0.5 6,729,837 + -0.8 6,729,837 + +1.1 6,729,837 + +0.1 6,729,837 + -1.9 6,729,837 + +1.8 6,729,838 - -0.5 6,729,838 - -0.2 6,730,131 - Pf6N2E2_5911 0.28 -3.4 6,730,145 + Pf6N2E2_5911 0.29 -1.5 6,730,145 + Pf6N2E2_5911 0.29 -1.1 6,730,146 - Pf6N2E2_5911 0.29 -0.9 6,730,146 - Pf6N2E2_5911 0.29 -0.8 6,730,169 + Pf6N2E2_5911 0.31 +0.5 6,730,169 + Pf6N2E2_5911 0.31 +0.0 6,730,170 - Pf6N2E2_5911 0.31 -0.5 6,730,170 - Pf6N2E2_5911 0.31 -0.6 6,730,170 - Pf6N2E2_5911 0.31 -0.2 6,730,170 - Pf6N2E2_5911 0.31 +0.1 6,730,170 - Pf6N2E2_5911 0.31 -0.7 6,730,173 + Pf6N2E2_5911 0.31 -1.3 6,730,174 - Pf6N2E2_5911 0.31 -0.1 6,730,193 + Pf6N2E2_5911 0.33 -0.8 6,730,193 + Pf6N2E2_5911 0.33 -0.8 6,730,193 + Pf6N2E2_5911 0.33 +0.5 6,730,552 + Pf6N2E2_5911 0.59 +0.4 6,730,552 + Pf6N2E2_5911 0.59 -0.1 6,730,552 + Pf6N2E2_5911 0.59 +0.2 6,730,553 - Pf6N2E2_5911 0.59 +0.1 6,730,553 - Pf6N2E2_5911 0.59 -1.0 6,730,555 + Pf6N2E2_5911 0.59 -0.7 6,730,567 + Pf6N2E2_5911 0.60 -1.5 6,730,567 + Pf6N2E2_5911 0.60 -1.0 6,730,585 + Pf6N2E2_5911 0.61 -0.0 6,730,601 + Pf6N2E2_5911 0.63 +0.5 6,730,601 + Pf6N2E2_5911 0.63 +1.5 6,730,601 + Pf6N2E2_5911 0.63 -1.3 6,730,601 + Pf6N2E2_5911 0.63 -0.8 6,730,601 + Pf6N2E2_5911 0.63 +0.5 6,730,602 - Pf6N2E2_5911 0.63 -0.1 6,730,602 - Pf6N2E2_5911 0.63 -0.0 6,730,602 - Pf6N2E2_5911 0.63 +1.5 6,730,673 + Pf6N2E2_5911 0.68 -0.8 6,730,674 - Pf6N2E2_5911 0.68 +1.5 6,730,674 - Pf6N2E2_5911 0.68 -0.1 6,730,700 + Pf6N2E2_5911 0.70 -3.0 6,730,700 + Pf6N2E2_5911 0.70 -1.7 6,730,701 - Pf6N2E2_5911 0.70 -0.3 6,730,766 + Pf6N2E2_5911 0.75 -0.3 6,730,766 + Pf6N2E2_5911 0.75 -2.9 6,730,766 + Pf6N2E2_5911 0.75 +1.5 6,730,766 + Pf6N2E2_5911 0.75 -0.8 6,730,767 - Pf6N2E2_5911 0.75 -0.4 6,730,928 + Pf6N2E2_5911 0.87 +0.5 6,730,929 - Pf6N2E2_5911 0.87 -1.0 6,730,972 + Pf6N2E2_5911 0.90 -1.3 6,730,973 - Pf6N2E2_5911 0.90 +0.4 6,731,013 + +1.5 6,731,013 + -0.5 6,731,013 + +0.1 6,731,024 - -0.5 6,731,063 + +0.1 6,731,072 + +1.4 6,731,073 - -1.5 6,731,108 + -0.8 6,731,109 - -1.4 6,731,109 - -2.0 6,731,242 + Pf6N2E2_5912 0.26 -0.8 6,731,248 + Pf6N2E2_5912 0.27 -0.5 6,731,248 + Pf6N2E2_5912 0.27 -1.2 6,731,248 + Pf6N2E2_5912 0.27 +0.5 6,731,248 + Pf6N2E2_5912 0.27 -1.1 6,731,249 - Pf6N2E2_5912 0.27 +0.6 6,731,249 - Pf6N2E2_5912 0.27 -0.0 6,731,249 - Pf6N2E2_5912 0.27 +0.1 6,731,249 - Pf6N2E2_5912 0.27 +1.5 6,731,258 + Pf6N2E2_5912 0.29 -0.5 6,731,258 + Pf6N2E2_5912 0.29 +0.8 6,731,259 - Pf6N2E2_5912 0.29 -1.0 6,731,310 + Pf6N2E2_5912 0.40 -0.6 6,731,310 + Pf6N2E2_5912 0.40 +0.1 6,731,311 - Pf6N2E2_5912 0.40 +1.5 6,731,311 - Pf6N2E2_5912 0.40 +1.5 6,731,374 + Pf6N2E2_5912 0.53 -1.9 6,731,374 + Pf6N2E2_5912 0.53 +0.1 6,731,375 - Pf6N2E2_5912 0.53 -0.0 6,731,375 - Pf6N2E2_5912 0.53 -0.2 6,731,479 + Pf6N2E2_5912 0.75 -0.7 6,731,479 + Pf6N2E2_5912 0.75 +0.8 6,731,480 - Pf6N2E2_5912 0.75 -0.1 6,731,480 - Pf6N2E2_5912 0.75 -0.9 6,731,480 - Pf6N2E2_5912 0.75 -0.2 6,731,508 + Pf6N2E2_5912 0.81 +0.9 6,731,625 + -0.6 6,731,625 + -0.7 6,731,626 - -0.6 6,731,626 - +0.5 6,731,626 - -1.6 6,731,700 + Pf6N2E2_5913 0.21 -0.6 6,731,700 + Pf6N2E2_5913 0.21 -0.3 6,731,700 + Pf6N2E2_5913 0.21 -0.8 6,731,701 - Pf6N2E2_5913 0.21 -0.6 6,731,701 - Pf6N2E2_5913 0.21 -0.2 6,731,701 - Pf6N2E2_5913 0.21 +0.5 6,731,765 + Pf6N2E2_5913 0.34 -0.1 6,731,766 - Pf6N2E2_5913 0.34 -1.0 6,731,871 + Pf6N2E2_5913 0.55 +1.6 6,731,872 - Pf6N2E2_5913 0.55 -2.7 6,731,872 - Pf6N2E2_5913 0.55 -1.4 6,731,947 - Pf6N2E2_5913 0.71 -1.0 6,732,011 + Pf6N2E2_5913 0.83 +1.5 6,732,012 - Pf6N2E2_5913 0.84 +0.4 6,732,095 - -0.6
Or see this region's nucleotide sequence