Experiment: nitrogen source nitrite 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3604 and Pf6N2E2_3605 are separated by 28 nucleotides Pf6N2E2_3605 and Pf6N2E2_3606 are separated by 306 nucleotides Pf6N2E2_3606 and Pf6N2E2_3607 overlap by 4 nucleotides
Pf6N2E2_3604 - SSU ribosomal protein S18p @ SSU ribosomal protein S18p, zinc-independent, at 4,195,474 to 4,195,704
_3604
Pf6N2E2_3605 - SSU ribosomal protein S6p, at 4,195,733 to 4,196,161
_3605
Pf6N2E2_3606 - 23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-), at 4,196,468 to 4,197,226
_3606
Pf6N2E2_3607 - 3'-to-5' exoribonuclease RNase R, at 4,197,223 to 4,199,853
_3607
Position (kb)
4196
4197
4198 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4196.251 kb on - strand at 4196.251 kb on - strand at 4196.323 kb on - strand at 4196.418 kb on + strand at 4196.418 kb on + strand at 4196.419 kb on - strand at 4196.419 kb on - strand at 4196.456 kb on + strand at 4196.456 kb on + strand at 4196.457 kb on - strand at 4196.457 kb on - strand at 4196.457 kb on - strand at 4196.457 kb on - strand at 4196.590 kb on - strand, within Pf6N2E2_3606 at 4196.676 kb on + strand, within Pf6N2E2_3606 at 4196.676 kb on + strand, within Pf6N2E2_3606 at 4196.676 kb on + strand, within Pf6N2E2_3606 at 4196.676 kb on + strand, within Pf6N2E2_3606 at 4196.676 kb on + strand, within Pf6N2E2_3606 at 4196.676 kb on + strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.677 kb on - strand, within Pf6N2E2_3606 at 4196.680 kb on + strand, within Pf6N2E2_3606 at 4196.680 kb on + strand, within Pf6N2E2_3606 at 4196.680 kb on + strand, within Pf6N2E2_3606 at 4196.681 kb on - strand, within Pf6N2E2_3606 at 4196.681 kb on - strand, within Pf6N2E2_3606 at 4196.681 kb on - strand, within Pf6N2E2_3606 at 4196.681 kb on - strand, within Pf6N2E2_3606 at 4196.681 kb on - strand, within Pf6N2E2_3606 at 4196.843 kb on - strand, within Pf6N2E2_3606 at 4196.902 kb on + strand, within Pf6N2E2_3606 at 4197.005 kb on + strand, within Pf6N2E2_3606 at 4197.006 kb on - strand, within Pf6N2E2_3606 at 4197.166 kb on + strand at 4197.238 kb on - strand at 4197.238 kb on - strand at 4197.295 kb on - strand at 4197.303 kb on + strand at 4197.303 kb on + strand at 4197.303 kb on + strand at 4197.303 kb on + strand at 4197.303 kb on + strand at 4197.304 kb on - strand at 4197.304 kb on - strand at 4197.304 kb on - strand at 4197.304 kb on - strand at 4197.361 kb on - strand at 4197.538 kb on - strand, within Pf6N2E2_3607 at 4197.740 kb on + strand, within Pf6N2E2_3607 at 4197.740 kb on + strand, within Pf6N2E2_3607 at 4197.741 kb on - strand, within Pf6N2E2_3607 at 4197.741 kb on - strand, within Pf6N2E2_3607 at 4197.741 kb on - strand, within Pf6N2E2_3607 at 4197.741 kb on - strand, within Pf6N2E2_3607 at 4197.743 kb on + strand, within Pf6N2E2_3607 at 4197.744 kb on - strand, within Pf6N2E2_3607 at 4197.744 kb on - strand, within Pf6N2E2_3607 at 4197.744 kb on - strand, within Pf6N2E2_3607 at 4197.785 kb on + strand, within Pf6N2E2_3607 at 4197.785 kb on + strand, within Pf6N2E2_3607 at 4197.785 kb on + strand, within Pf6N2E2_3607 at 4197.785 kb on + strand, within Pf6N2E2_3607 at 4197.786 kb on - strand, within Pf6N2E2_3607 at 4197.786 kb on - strand, within Pf6N2E2_3607 at 4197.786 kb on - strand, within Pf6N2E2_3607 at 4197.980 kb on + strand, within Pf6N2E2_3607 at 4197.981 kb on - strand, within Pf6N2E2_3607 at 4198.118 kb on + strand, within Pf6N2E2_3607 at 4198.119 kb on - strand, within Pf6N2E2_3607 at 4198.127 kb on + strand, within Pf6N2E2_3607 at 4198.163 kb on + strand, within Pf6N2E2_3607 at 4198.164 kb on - strand, within Pf6N2E2_3607
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM remove 4,196,251 - -0.2 4,196,251 - +1.4 4,196,323 - +0.5 4,196,418 + +0.5 4,196,418 + +0.7 4,196,419 - +0.9 4,196,419 - -0.1 4,196,456 + -0.5 4,196,456 + -0.1 4,196,457 - -0.2 4,196,457 - +1.8 4,196,457 - -0.0 4,196,457 - +0.3 4,196,590 - Pf6N2E2_3606 0.16 -1.0 4,196,676 + Pf6N2E2_3606 0.27 -1.1 4,196,676 + Pf6N2E2_3606 0.27 +0.0 4,196,676 + Pf6N2E2_3606 0.27 -1.1 4,196,676 + Pf6N2E2_3606 0.27 -1.0 4,196,676 + Pf6N2E2_3606 0.27 -0.9 4,196,676 + Pf6N2E2_3606 0.27 -0.6 4,196,677 - Pf6N2E2_3606 0.28 +0.5 4,196,677 - Pf6N2E2_3606 0.28 -0.6 4,196,677 - Pf6N2E2_3606 0.28 +0.4 4,196,677 - Pf6N2E2_3606 0.28 +0.1 4,196,677 - Pf6N2E2_3606 0.28 -0.4 4,196,677 - Pf6N2E2_3606 0.28 +0.3 4,196,677 - Pf6N2E2_3606 0.28 -2.1 4,196,677 - Pf6N2E2_3606 0.28 -0.6 4,196,677 - Pf6N2E2_3606 0.28 -1.0 4,196,680 + Pf6N2E2_3606 0.28 -0.3 4,196,680 + Pf6N2E2_3606 0.28 +0.2 4,196,680 + Pf6N2E2_3606 0.28 +0.5 4,196,681 - Pf6N2E2_3606 0.28 +1.0 4,196,681 - Pf6N2E2_3606 0.28 +0.8 4,196,681 - Pf6N2E2_3606 0.28 +0.5 4,196,681 - Pf6N2E2_3606 0.28 -0.1 4,196,681 - Pf6N2E2_3606 0.28 -0.5 4,196,843 - Pf6N2E2_3606 0.49 -0.1 4,196,902 + Pf6N2E2_3606 0.57 -1.1 4,197,005 + Pf6N2E2_3606 0.71 -0.7 4,197,006 - Pf6N2E2_3606 0.71 -0.3 4,197,166 + -0.6 4,197,238 - -0.4 4,197,238 - -2.7 4,197,295 - -2.3 4,197,303 + -0.8 4,197,303 + -1.6 4,197,303 + -0.5 4,197,303 + -1.1 4,197,303 + -1.5 4,197,304 - +2.6 4,197,304 - -0.3 4,197,304 - +0.3 4,197,304 - +1.5 4,197,361 - -1.7 4,197,538 - Pf6N2E2_3607 0.12 -1.0 4,197,740 + Pf6N2E2_3607 0.20 -0.8 4,197,740 + Pf6N2E2_3607 0.20 +0.5 4,197,741 - Pf6N2E2_3607 0.20 -0.4 4,197,741 - Pf6N2E2_3607 0.20 -1.1 4,197,741 - Pf6N2E2_3607 0.20 -2.1 4,197,741 - Pf6N2E2_3607 0.20 -2.3 4,197,743 + Pf6N2E2_3607 0.20 -1.1 4,197,744 - Pf6N2E2_3607 0.20 -3.4 4,197,744 - Pf6N2E2_3607 0.20 -2.6 4,197,744 - Pf6N2E2_3607 0.20 -0.1 4,197,785 + Pf6N2E2_3607 0.21 -0.8 4,197,785 + Pf6N2E2_3607 0.21 -1.8 4,197,785 + Pf6N2E2_3607 0.21 +0.0 4,197,785 + Pf6N2E2_3607 0.21 -0.1 4,197,786 - Pf6N2E2_3607 0.21 -1.8 4,197,786 - Pf6N2E2_3607 0.21 +0.4 4,197,786 - Pf6N2E2_3607 0.21 +0.0 4,197,980 + Pf6N2E2_3607 0.29 -2.0 4,197,981 - Pf6N2E2_3607 0.29 -1.9 4,198,118 + Pf6N2E2_3607 0.34 +1.5 4,198,119 - Pf6N2E2_3607 0.34 +0.5 4,198,127 + Pf6N2E2_3607 0.34 -0.8 4,198,163 + Pf6N2E2_3607 0.36 -1.7 4,198,164 - Pf6N2E2_3607 0.36 +1.5
Or see this region's nucleotide sequence