Experiment: nitrogen source nitrite 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1895 and Pf6N2E2_1896 are separated by 212 nucleotides Pf6N2E2_1896 and Pf6N2E2_1897 are separated by 79 nucleotides Pf6N2E2_1897 and Pf6N2E2_1898 overlap by 4 nucleotides
Pf6N2E2_1895 - PQQ-dependent oxidoreductase, gdhB family, at 2,245,833 to 2,246,951
_1895
Pf6N2E2_1896 - hypothetical protein, at 2,247,164 to 2,247,286
_1896
Pf6N2E2_1897 - Cytochrome B561, at 2,247,366 to 2,247,944
_1897
Pf6N2E2_1898 - Catalase (EC 1.11.1.6), at 2,247,941 to 2,249,023
_1898
Position (kb)
2247
2248 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2246.444 kb on - strand, within Pf6N2E2_1895 at 2246.606 kb on + strand, within Pf6N2E2_1895 at 2246.606 kb on + strand, within Pf6N2E2_1895 at 2246.648 kb on - strand, within Pf6N2E2_1895 at 2246.992 kb on + strand at 2247.005 kb on - strand at 2247.005 kb on - strand at 2247.014 kb on + strand at 2247.014 kb on + strand at 2247.028 kb on - strand at 2247.151 kb on + strand at 2247.152 kb on - strand at 2247.204 kb on - strand, within Pf6N2E2_1896 at 2247.204 kb on - strand, within Pf6N2E2_1896 at 2247.204 kb on - strand, within Pf6N2E2_1896 at 2247.289 kb on + strand at 2247.290 kb on - strand at 2247.290 kb on - strand at 2247.305 kb on + strand at 2247.524 kb on + strand, within Pf6N2E2_1897 at 2247.524 kb on + strand, within Pf6N2E2_1897 at 2247.524 kb on + strand, within Pf6N2E2_1897 at 2247.524 kb on + strand, within Pf6N2E2_1897 at 2247.525 kb on - strand, within Pf6N2E2_1897 at 2247.664 kb on + strand, within Pf6N2E2_1897 at 2247.664 kb on + strand, within Pf6N2E2_1897 at 2247.664 kb on + strand, within Pf6N2E2_1897 at 2247.665 kb on - strand, within Pf6N2E2_1897 at 2247.665 kb on - strand, within Pf6N2E2_1897 at 2247.665 kb on - strand, within Pf6N2E2_1897 at 2247.824 kb on - strand, within Pf6N2E2_1897 at 2247.824 kb on - strand, within Pf6N2E2_1897 at 2247.994 kb on - strand at 2248.024 kb on - strand at 2248.252 kb on + strand, within Pf6N2E2_1898 at 2248.253 kb on - strand, within Pf6N2E2_1898 at 2248.352 kb on + strand, within Pf6N2E2_1898 at 2248.352 kb on + strand, within Pf6N2E2_1898 at 2248.352 kb on + strand, within Pf6N2E2_1898 at 2248.435 kb on - strand, within Pf6N2E2_1898 at 2248.449 kb on + strand, within Pf6N2E2_1898 at 2248.572 kb on + strand, within Pf6N2E2_1898 at 2248.741 kb on - strand, within Pf6N2E2_1898 at 2248.741 kb on - strand, within Pf6N2E2_1898 at 2248.819 kb on + strand, within Pf6N2E2_1898 at 2248.820 kb on - strand, within Pf6N2E2_1898 at 2248.930 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM remove 2,246,444 - Pf6N2E2_1895 0.55 -0.0 2,246,606 + Pf6N2E2_1895 0.69 +0.5 2,246,606 + Pf6N2E2_1895 0.69 -1.7 2,246,648 - Pf6N2E2_1895 0.73 -0.1 2,246,992 + -1.9 2,247,005 - +0.5 2,247,005 - +0.4 2,247,014 + -0.4 2,247,014 + -1.2 2,247,028 - -0.3 2,247,151 + -0.4 2,247,152 - +1.5 2,247,204 - Pf6N2E2_1896 0.33 -0.5 2,247,204 - Pf6N2E2_1896 0.33 +0.5 2,247,204 - Pf6N2E2_1896 0.33 +0.2 2,247,289 + +2.0 2,247,290 - -0.2 2,247,290 - -1.2 2,247,305 + -1.3 2,247,524 + Pf6N2E2_1897 0.27 -2.3 2,247,524 + Pf6N2E2_1897 0.27 -0.2 2,247,524 + Pf6N2E2_1897 0.27 -1.1 2,247,524 + Pf6N2E2_1897 0.27 +0.1 2,247,525 - Pf6N2E2_1897 0.27 +0.5 2,247,664 + Pf6N2E2_1897 0.51 -0.5 2,247,664 + Pf6N2E2_1897 0.51 -0.4 2,247,664 + Pf6N2E2_1897 0.51 -2.3 2,247,665 - Pf6N2E2_1897 0.52 -1.4 2,247,665 - Pf6N2E2_1897 0.52 +0.9 2,247,665 - Pf6N2E2_1897 0.52 -0.8 2,247,824 - Pf6N2E2_1897 0.79 +0.5 2,247,824 - Pf6N2E2_1897 0.79 -1.4 2,247,994 - -0.9 2,248,024 - +1.5 2,248,252 + Pf6N2E2_1898 0.29 +0.5 2,248,253 - Pf6N2E2_1898 0.29 -1.3 2,248,352 + Pf6N2E2_1898 0.38 +0.2 2,248,352 + Pf6N2E2_1898 0.38 -0.6 2,248,352 + Pf6N2E2_1898 0.38 -1.3 2,248,435 - Pf6N2E2_1898 0.46 -0.1 2,248,449 + Pf6N2E2_1898 0.47 +0.4 2,248,572 + Pf6N2E2_1898 0.58 -0.5 2,248,741 - Pf6N2E2_1898 0.74 -2.3 2,248,741 - Pf6N2E2_1898 0.74 -0.7 2,248,819 + Pf6N2E2_1898 0.81 -0.8 2,248,820 - Pf6N2E2_1898 0.81 -1.1 2,248,930 - -0.2
Or see this region's nucleotide sequence