Experiment: nitrogen source nitrite 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1500 and Pf6N2E2_1501 are separated by 34 nucleotides Pf6N2E2_1501 and Pf6N2E2_1502 are separated by 111 nucleotides Pf6N2E2_1502 and Pf6N2E2_1503 are separated by 20 nucleotides Pf6N2E2_1503 and Pf6N2E2_1504 are separated by 109 nucleotides Pf6N2E2_1504 and Pf6N2E2_1505 are separated by 28 nucleotides
Pf6N2E2_1500 - LysR family transcriptional regulator lrhA, at 1,754,098 to 1,754,955
_1500
Pf6N2E2_1501 - Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) (EC 3.6.1.17), at 1,754,990 to 1,755,433
_1501
Pf6N2E2_1502 - Two-component system regulatory protein, at 1,755,545 to 1,755,922
_1502
Pf6N2E2_1503 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 1,755,943 to 1,758,126
_1503
Pf6N2E2_1504 - putative 2-pyrone-4,6-dicarboxylic acid hydrolase, at 1,758,236 to 1,759,066
_1504
Pf6N2E2_1505 - Transporter, MFS superfamily, at 1,759,095 to 1,760,291
_1505
Position (kb)
1755
1756
1757
1758
1759 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1754.979 kb on - strand at 1755.123 kb on + strand, within Pf6N2E2_1501 at 1755.123 kb on + strand, within Pf6N2E2_1501 at 1755.123 kb on + strand, within Pf6N2E2_1501 at 1755.124 kb on - strand, within Pf6N2E2_1501 at 1755.355 kb on - strand, within Pf6N2E2_1501 at 1755.414 kb on + strand at 1755.414 kb on + strand at 1755.414 kb on + strand at 1755.454 kb on + strand at 1755.454 kb on + strand at 1755.486 kb on + strand at 1755.486 kb on + strand at 1755.486 kb on + strand at 1755.486 kb on + strand at 1755.487 kb on - strand at 1755.715 kb on + strand, within Pf6N2E2_1502 at 1755.715 kb on + strand, within Pf6N2E2_1502 at 1755.932 kb on + strand at 1755.933 kb on - strand at 1756.001 kb on + strand at 1756.005 kb on + strand at 1756.005 kb on + strand at 1756.005 kb on + strand at 1756.005 kb on + strand at 1756.006 kb on - strand at 1756.199 kb on + strand, within Pf6N2E2_1503 at 1756.199 kb on + strand, within Pf6N2E2_1503 at 1756.240 kb on - strand, within Pf6N2E2_1503 at 1756.240 kb on - strand, within Pf6N2E2_1503 at 1756.240 kb on - strand, within Pf6N2E2_1503 at 1756.759 kb on - strand, within Pf6N2E2_1503 at 1756.839 kb on - strand, within Pf6N2E2_1503 at 1756.839 kb on - strand, within Pf6N2E2_1503 at 1756.992 kb on + strand, within Pf6N2E2_1503 at 1756.992 kb on + strand, within Pf6N2E2_1503 at 1757.078 kb on + strand, within Pf6N2E2_1503 at 1757.078 kb on + strand, within Pf6N2E2_1503 at 1757.078 kb on + strand, within Pf6N2E2_1503 at 1757.078 kb on + strand, within Pf6N2E2_1503 at 1757.079 kb on - strand, within Pf6N2E2_1503 at 1757.079 kb on - strand, within Pf6N2E2_1503 at 1757.079 kb on - strand, within Pf6N2E2_1503 at 1757.079 kb on - strand, within Pf6N2E2_1503 at 1757.079 kb on - strand, within Pf6N2E2_1503 at 1757.079 kb on - strand, within Pf6N2E2_1503 at 1757.079 kb on - strand, within Pf6N2E2_1503 at 1757.079 kb on - strand, within Pf6N2E2_1503 at 1757.079 kb on - strand, within Pf6N2E2_1503 at 1757.082 kb on + strand, within Pf6N2E2_1503 at 1757.083 kb on - strand, within Pf6N2E2_1503 at 1757.111 kb on + strand, within Pf6N2E2_1503 at 1757.112 kb on - strand, within Pf6N2E2_1503 at 1757.112 kb on - strand, within Pf6N2E2_1503 at 1757.150 kb on + strand, within Pf6N2E2_1503 at 1757.150 kb on + strand, within Pf6N2E2_1503 at 1757.150 kb on + strand, within Pf6N2E2_1503 at 1757.150 kb on + strand, within Pf6N2E2_1503 at 1757.150 kb on + strand, within Pf6N2E2_1503 at 1757.276 kb on + strand, within Pf6N2E2_1503 at 1757.276 kb on + strand, within Pf6N2E2_1503 at 1757.276 kb on + strand, within Pf6N2E2_1503 at 1757.277 kb on - strand, within Pf6N2E2_1503 at 1757.277 kb on - strand, within Pf6N2E2_1503 at 1757.277 kb on - strand, within Pf6N2E2_1503 at 1757.344 kb on + strand, within Pf6N2E2_1503 at 1757.345 kb on - strand, within Pf6N2E2_1503 at 1757.361 kb on + strand, within Pf6N2E2_1503 at 1757.544 kb on - strand, within Pf6N2E2_1503 at 1757.544 kb on - strand, within Pf6N2E2_1503 at 1757.889 kb on + strand, within Pf6N2E2_1503 at 1757.890 kb on - strand, within Pf6N2E2_1503 at 1757.966 kb on - strand at 1757.988 kb on - strand at 1757.988 kb on - strand at 1757.988 kb on - strand at 1758.020 kb on + strand at 1758.021 kb on - strand at 1758.021 kb on - strand at 1758.078 kb on - strand at 1758.107 kb on - strand at 1758.181 kb on + strand at 1758.181 kb on + strand at 1758.182 kb on - strand at 1758.182 kb on - strand at 1758.182 kb on - strand at 1758.182 kb on - strand at 1758.182 kb on - strand at 1758.182 kb on - strand at 1758.189 kb on + strand at 1758.190 kb on - strand at 1758.472 kb on - strand, within Pf6N2E2_1504 at 1758.943 kb on - strand, within Pf6N2E2_1504 at 1758.943 kb on - strand, within Pf6N2E2_1504 at 1758.963 kb on + strand, within Pf6N2E2_1504 at 1758.963 kb on + strand, within Pf6N2E2_1504 at 1759.048 kb on + strand at 1759.051 kb on + strand at 1759.051 kb on + strand at 1759.051 kb on + strand at 1759.051 kb on + strand at 1759.052 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 1mM remove 1,754,979 - -0.1 1,755,123 + Pf6N2E2_1501 0.30 -0.1 1,755,123 + Pf6N2E2_1501 0.30 -0.9 1,755,123 + Pf6N2E2_1501 0.30 -2.2 1,755,124 - Pf6N2E2_1501 0.30 -0.7 1,755,355 - Pf6N2E2_1501 0.82 +1.5 1,755,414 + -0.1 1,755,414 + +0.0 1,755,414 + +1.0 1,755,454 + -0.9 1,755,454 + -0.1 1,755,486 + +1.0 1,755,486 + -1.8 1,755,486 + -0.6 1,755,486 + -0.7 1,755,487 - +0.4 1,755,715 + Pf6N2E2_1502 0.45 -0.1 1,755,715 + Pf6N2E2_1502 0.45 -1.4 1,755,932 + -0.1 1,755,933 - -0.4 1,756,001 + -0.3 1,756,005 + -0.9 1,756,005 + -1.5 1,756,005 + +1.5 1,756,005 + -1.5 1,756,006 - +1.5 1,756,199 + Pf6N2E2_1503 0.12 -0.6 1,756,199 + Pf6N2E2_1503 0.12 -0.5 1,756,240 - Pf6N2E2_1503 0.14 +1.5 1,756,240 - Pf6N2E2_1503 0.14 +0.6 1,756,240 - Pf6N2E2_1503 0.14 -0.1 1,756,759 - Pf6N2E2_1503 0.37 -0.3 1,756,839 - Pf6N2E2_1503 0.41 +1.5 1,756,839 - Pf6N2E2_1503 0.41 -0.2 1,756,992 + Pf6N2E2_1503 0.48 -0.5 1,756,992 + Pf6N2E2_1503 0.48 -0.5 1,757,078 + Pf6N2E2_1503 0.52 +0.5 1,757,078 + Pf6N2E2_1503 0.52 +1.1 1,757,078 + Pf6N2E2_1503 0.52 -1.1 1,757,078 + Pf6N2E2_1503 0.52 -1.2 1,757,079 - Pf6N2E2_1503 0.52 -1.1 1,757,079 - Pf6N2E2_1503 0.52 -0.7 1,757,079 - Pf6N2E2_1503 0.52 +0.5 1,757,079 - Pf6N2E2_1503 0.52 -0.5 1,757,079 - Pf6N2E2_1503 0.52 -0.8 1,757,079 - Pf6N2E2_1503 0.52 -0.1 1,757,079 - Pf6N2E2_1503 0.52 -0.1 1,757,079 - Pf6N2E2_1503 0.52 +0.1 1,757,079 - Pf6N2E2_1503 0.52 -1.0 1,757,082 + Pf6N2E2_1503 0.52 +0.2 1,757,083 - Pf6N2E2_1503 0.52 +0.2 1,757,111 + Pf6N2E2_1503 0.53 -0.7 1,757,112 - Pf6N2E2_1503 0.54 -1.3 1,757,112 - Pf6N2E2_1503 0.54 +1.5 1,757,150 + Pf6N2E2_1503 0.55 -1.6 1,757,150 + Pf6N2E2_1503 0.55 +2.1 1,757,150 + Pf6N2E2_1503 0.55 -0.4 1,757,150 + Pf6N2E2_1503 0.55 +0.5 1,757,150 + Pf6N2E2_1503 0.55 -1.3 1,757,276 + Pf6N2E2_1503 0.61 -0.9 1,757,276 + Pf6N2E2_1503 0.61 -0.1 1,757,276 + Pf6N2E2_1503 0.61 +0.9 1,757,277 - Pf6N2E2_1503 0.61 -2.5 1,757,277 - Pf6N2E2_1503 0.61 -2.8 1,757,277 - Pf6N2E2_1503 0.61 -1.1 1,757,344 + Pf6N2E2_1503 0.64 -0.9 1,757,345 - Pf6N2E2_1503 0.64 -0.5 1,757,361 + Pf6N2E2_1503 0.65 -0.8 1,757,544 - Pf6N2E2_1503 0.73 -0.5 1,757,544 - Pf6N2E2_1503 0.73 +1.5 1,757,889 + Pf6N2E2_1503 0.89 -2.7 1,757,890 - Pf6N2E2_1503 0.89 +1.5 1,757,966 - -0.8 1,757,988 - -1.8 1,757,988 - +1.5 1,757,988 - -2.8 1,758,020 + +0.4 1,758,021 - +0.5 1,758,021 - +0.8 1,758,078 - +2.4 1,758,107 - -0.1 1,758,181 + +1.5 1,758,181 + -1.5 1,758,182 - +0.2 1,758,182 - -0.4 1,758,182 - +1.5 1,758,182 - -0.1 1,758,182 - -0.8 1,758,182 - +0.8 1,758,189 + -0.8 1,758,190 - +1.5 1,758,472 - Pf6N2E2_1504 0.28 -0.1 1,758,943 - Pf6N2E2_1504 0.85 +1.5 1,758,943 - Pf6N2E2_1504 0.85 +1.0 1,758,963 + Pf6N2E2_1504 0.87 -0.1 1,758,963 + Pf6N2E2_1504 0.87 +0.2 1,759,048 + -2.0 1,759,051 + +0.4 1,759,051 + +0.6 1,759,051 + +1.5 1,759,051 + +0.1 1,759,052 - -1.7
Or see this region's nucleotide sequence