Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_836

Experiment: nitrogen source nitrite 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_835 and Pf6N2E2_836 are separated by 231 nucleotidesPf6N2E2_836 and Pf6N2E2_837 are separated by 7 nucleotides Pf6N2E2_835 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179), at 976,137 to 977,429 _835 Pf6N2E2_836 - ABC-type protease/lipase transport system, ATPase and permease components, at 977,661 to 979,376 _836 Pf6N2E2_837 - HlyD family secretion protein, at 979,384 to 980,706 _837 Position (kb) 977 978 979 980Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 976.733 kb on + strand, within Pf6N2E2_835at 976.733 kb on + strand, within Pf6N2E2_835at 976.733 kb on + strand, within Pf6N2E2_835at 976.733 kb on + strand, within Pf6N2E2_835at 976.733 kb on + strand, within Pf6N2E2_835at 976.734 kb on - strand, within Pf6N2E2_835at 976.734 kb on - strand, within Pf6N2E2_835at 977.078 kb on + strand, within Pf6N2E2_835at 977.079 kb on - strand, within Pf6N2E2_835at 977.171 kb on + strand, within Pf6N2E2_835at 977.171 kb on + strand, within Pf6N2E2_835at 977.171 kb on + strand, within Pf6N2E2_835at 977.172 kb on - strand, within Pf6N2E2_835at 977.181 kb on - strand, within Pf6N2E2_835at 977.477 kb on + strandat 977.542 kb on + strandat 977.542 kb on + strandat 977.542 kb on + strandat 977.587 kb on - strandat 977.714 kb on + strandat 977.778 kb on + strandat 977.778 kb on + strandat 977.779 kb on - strandat 977.779 kb on - strandat 977.779 kb on - strandat 977.779 kb on - strandat 977.794 kb on - strandat 977.982 kb on - strand, within Pf6N2E2_836at 977.982 kb on - strand, within Pf6N2E2_836at 978.126 kb on + strand, within Pf6N2E2_836at 978.127 kb on - strand, within Pf6N2E2_836at 978.442 kb on - strand, within Pf6N2E2_836at 978.442 kb on - strand, within Pf6N2E2_836at 978.442 kb on - strand, within Pf6N2E2_836at 978.571 kb on - strand, within Pf6N2E2_836at 978.571 kb on - strand, within Pf6N2E2_836at 978.823 kb on + strand, within Pf6N2E2_836at 978.876 kb on + strand, within Pf6N2E2_836at 978.877 kb on - strand, within Pf6N2E2_836at 979.026 kb on + strand, within Pf6N2E2_836at 979.104 kb on + strand, within Pf6N2E2_836at 979.104 kb on + strand, within Pf6N2E2_836at 979.375 kb on - strandat 979.966 kb on + strand, within Pf6N2E2_837at 979.967 kb on - strand, within Pf6N2E2_837at 979.967 kb on - strand, within Pf6N2E2_837at 979.979 kb on + strand, within Pf6N2E2_837at 980.012 kb on - strand, within Pf6N2E2_837at 980.012 kb on - strand, within Pf6N2E2_837at 980.072 kb on + strand, within Pf6N2E2_837at 980.203 kb on + strand, within Pf6N2E2_837at 980.203 kb on + strand, within Pf6N2E2_837at 980.203 kb on + strand, within Pf6N2E2_837at 980.203 kb on + strand, within Pf6N2E2_837at 980.203 kb on + strand, within Pf6N2E2_837at 980.204 kb on - strand, within Pf6N2E2_837at 980.204 kb on - strand, within Pf6N2E2_837at 980.257 kb on - strand, within Pf6N2E2_837

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM
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976,733 + Pf6N2E2_835 0.46 -1.3
976,733 + Pf6N2E2_835 0.46 -1.8
976,733 + Pf6N2E2_835 0.46 +1.6
976,733 + Pf6N2E2_835 0.46 -0.8
976,733 + Pf6N2E2_835 0.46 -1.2
976,734 - Pf6N2E2_835 0.46 +0.5
976,734 - Pf6N2E2_835 0.46 -0.1
977,078 + Pf6N2E2_835 0.73 -0.4
977,079 - Pf6N2E2_835 0.73 -1.6
977,171 + Pf6N2E2_835 0.80 +0.7
977,171 + Pf6N2E2_835 0.80 +0.3
977,171 + Pf6N2E2_835 0.80 +1.3
977,172 - Pf6N2E2_835 0.80 -0.5
977,181 - Pf6N2E2_835 0.81 +0.4
977,477 + -0.4
977,542 + -1.0
977,542 + -0.8
977,542 + -1.0
977,587 - -0.6
977,714 + +0.6
977,778 + +1.6
977,778 + +1.6
977,779 - -3.0
977,779 - -1.5
977,779 - +0.4
977,779 - +1.6
977,794 - +1.6
977,982 - Pf6N2E2_836 0.19 -1.6
977,982 - Pf6N2E2_836 0.19 +0.6
978,126 + Pf6N2E2_836 0.27 -0.0
978,127 - Pf6N2E2_836 0.27 +0.6
978,442 - Pf6N2E2_836 0.46 +1.2
978,442 - Pf6N2E2_836 0.46 -0.1
978,442 - Pf6N2E2_836 0.46 +1.6
978,571 - Pf6N2E2_836 0.53 -0.2
978,571 - Pf6N2E2_836 0.53 +0.3
978,823 + Pf6N2E2_836 0.68 +1.1
978,876 + Pf6N2E2_836 0.71 +0.6
978,877 - Pf6N2E2_836 0.71 +1.2
979,026 + Pf6N2E2_836 0.80 -0.4
979,104 + Pf6N2E2_836 0.84 -0.9
979,104 + Pf6N2E2_836 0.84 +2.0
979,375 - -0.9
979,966 + Pf6N2E2_837 0.44 +0.2
979,967 - Pf6N2E2_837 0.44 -0.8
979,967 - Pf6N2E2_837 0.44 +0.9
979,979 + Pf6N2E2_837 0.45 +0.2
980,012 - Pf6N2E2_837 0.47 -0.0
980,012 - Pf6N2E2_837 0.47 -0.2
980,072 + Pf6N2E2_837 0.52 -2.0
980,203 + Pf6N2E2_837 0.62 +0.0
980,203 + Pf6N2E2_837 0.62 -0.8
980,203 + Pf6N2E2_837 0.62 -0.4
980,203 + Pf6N2E2_837 0.62 +0.3
980,203 + Pf6N2E2_837 0.62 +1.6
980,204 - Pf6N2E2_837 0.62 +3.2
980,204 - Pf6N2E2_837 0.62 -0.4
980,257 - Pf6N2E2_837 0.66 -0.1

Or see this region's nucleotide sequence