Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5906 and Pf6N2E2_5907 are separated by 158 nucleotides Pf6N2E2_5907 and Pf6N2E2_5908 are separated by 26 nucleotides Pf6N2E2_5908 and Pf6N2E2_5909 are separated by 2 nucleotides Pf6N2E2_5909 and Pf6N2E2_5910 are separated by 78 nucleotides Pf6N2E2_5910 and Pf6N2E2_5911 are separated by 25 nucleotides
Pf6N2E2_5906 - Flp pilus assembly protein, pilin Flp, at 6,726,430 to 6,726,654
_5906
Pf6N2E2_5907 - Flp pilus assembly protein, pilin Flp, at 6,726,813 to 6,727,007
_5907
Pf6N2E2_5908 - Type IV prepilin peptidase TadV/CpaA, at 6,727,034 to 6,727,564
_5908
Pf6N2E2_5909 - Predicted ATPase with chaperone activity, associated with Flp pilus assembly, at 6,727,567 to 6,728,895
_5909
Pf6N2E2_5910 - Flp pilus assembly protein RcpC/CpaB, at 6,728,974 to 6,729,723
_5910
Pf6N2E2_5911 - Von Willebrand factor type A domain protein, associated with Flp pilus assembly, at 6,729,749 to 6,731,110
_5911
Position (kb)
6727
6728
6729 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6726.687 kb on - strand at 6726.700 kb on + strand at 6726.727 kb on + strand at 6726.778 kb on + strand at 6726.778 kb on + strand at 6726.784 kb on - strand at 6726.829 kb on + strand at 6726.829 kb on + strand at 6726.829 kb on + strand at 6726.829 kb on + strand at 6726.829 kb on + strand at 6726.830 kb on - strand at 6727.172 kb on + strand, within Pf6N2E2_5908 at 6727.172 kb on + strand, within Pf6N2E2_5908 at 6727.173 kb on - strand, within Pf6N2E2_5908 at 6727.173 kb on - strand, within Pf6N2E2_5908 at 6727.173 kb on - strand, within Pf6N2E2_5908 at 6727.173 kb on - strand, within Pf6N2E2_5908 at 6727.173 kb on - strand, within Pf6N2E2_5908 at 6727.174 kb on + strand, within Pf6N2E2_5908 at 6727.175 kb on - strand, within Pf6N2E2_5908 at 6727.175 kb on - strand, within Pf6N2E2_5908 at 6727.206 kb on + strand, within Pf6N2E2_5908 at 6727.207 kb on - strand, within Pf6N2E2_5908 at 6727.207 kb on - strand, within Pf6N2E2_5908 at 6727.243 kb on + strand, within Pf6N2E2_5908 at 6727.244 kb on - strand, within Pf6N2E2_5908 at 6727.244 kb on - strand, within Pf6N2E2_5908 at 6727.247 kb on + strand, within Pf6N2E2_5908 at 6727.247 kb on + strand, within Pf6N2E2_5908 at 6727.247 kb on + strand, within Pf6N2E2_5908 at 6727.247 kb on + strand, within Pf6N2E2_5908 at 6727.247 kb on + strand, within Pf6N2E2_5908 at 6727.248 kb on - strand, within Pf6N2E2_5908 at 6727.248 kb on - strand, within Pf6N2E2_5908 at 6727.248 kb on - strand, within Pf6N2E2_5908 at 6727.248 kb on - strand, within Pf6N2E2_5908 at 6727.320 kb on - strand, within Pf6N2E2_5908 at 6727.445 kb on + strand, within Pf6N2E2_5908 at 6727.446 kb on - strand, within Pf6N2E2_5908 at 6727.446 kb on - strand, within Pf6N2E2_5908 at 6727.562 kb on + strand at 6727.563 kb on - strand at 6727.563 kb on - strand at 6727.563 kb on - strand at 6727.563 kb on - strand at 6727.838 kb on + strand, within Pf6N2E2_5909 at 6727.838 kb on + strand, within Pf6N2E2_5909 at 6727.839 kb on - strand, within Pf6N2E2_5909 at 6727.909 kb on + strand, within Pf6N2E2_5909 at 6727.910 kb on - strand, within Pf6N2E2_5909 at 6727.942 kb on + strand, within Pf6N2E2_5909 at 6727.942 kb on + strand, within Pf6N2E2_5909 at 6727.942 kb on + strand, within Pf6N2E2_5909 at 6727.943 kb on - strand, within Pf6N2E2_5909 at 6728.089 kb on + strand, within Pf6N2E2_5909 at 6728.089 kb on + strand, within Pf6N2E2_5909 at 6728.089 kb on + strand, within Pf6N2E2_5909 at 6728.089 kb on + strand, within Pf6N2E2_5909 at 6728.090 kb on - strand, within Pf6N2E2_5909 at 6728.090 kb on - strand, within Pf6N2E2_5909 at 6728.090 kb on - strand, within Pf6N2E2_5909 at 6728.090 kb on - strand, within Pf6N2E2_5909 at 6728.090 kb on - strand, within Pf6N2E2_5909 at 6728.090 kb on - strand, within Pf6N2E2_5909 at 6728.116 kb on + strand, within Pf6N2E2_5909 at 6728.116 kb on + strand, within Pf6N2E2_5909 at 6728.116 kb on + strand, within Pf6N2E2_5909 at 6728.117 kb on - strand, within Pf6N2E2_5909 at 6728.117 kb on - strand, within Pf6N2E2_5909 at 6728.117 kb on - strand, within Pf6N2E2_5909 at 6728.203 kb on + strand, within Pf6N2E2_5909 at 6728.204 kb on - strand, within Pf6N2E2_5909 at 6728.204 kb on - strand, within Pf6N2E2_5909 at 6728.353 kb on + strand, within Pf6N2E2_5909 at 6728.374 kb on + strand, within Pf6N2E2_5909 at 6728.617 kb on + strand, within Pf6N2E2_5909 at 6728.618 kb on - strand, within Pf6N2E2_5909 at 6728.618 kb on - strand, within Pf6N2E2_5909 at 6728.654 kb on - strand, within Pf6N2E2_5909 at 6728.698 kb on + strand, within Pf6N2E2_5909 at 6728.698 kb on + strand, within Pf6N2E2_5909 at 6728.719 kb on + strand, within Pf6N2E2_5909 at 6728.720 kb on - strand, within Pf6N2E2_5909 at 6728.720 kb on - strand, within Pf6N2E2_5909 at 6728.720 kb on - strand, within Pf6N2E2_5909 at 6728.720 kb on - strand, within Pf6N2E2_5909 at 6728.720 kb on - strand, within Pf6N2E2_5909 at 6728.737 kb on + strand, within Pf6N2E2_5909 at 6728.737 kb on + strand, within Pf6N2E2_5909 at 6728.737 kb on + strand, within Pf6N2E2_5909 at 6728.737 kb on + strand, within Pf6N2E2_5909 at 6728.737 kb on + strand, within Pf6N2E2_5909 at 6728.737 kb on + strand, within Pf6N2E2_5909 at 6728.738 kb on - strand, within Pf6N2E2_5909 at 6728.738 kb on - strand, within Pf6N2E2_5909 at 6728.738 kb on - strand, within Pf6N2E2_5909 at 6728.738 kb on - strand, within Pf6N2E2_5909 at 6728.812 kb on + strand at 6728.869 kb on + strand at 6728.869 kb on + strand at 6728.869 kb on + strand at 6728.869 kb on + strand at 6728.869 kb on + strand at 6728.869 kb on + strand at 6728.870 kb on - strand at 6728.870 kb on - strand at 6728.870 kb on - strand at 6728.870 kb on - strand at 6728.922 kb on + strand at 6728.922 kb on + strand at 6728.923 kb on - strand at 6729.156 kb on - strand, within Pf6N2E2_5910 at 6729.644 kb on + strand, within Pf6N2E2_5910 at 6729.645 kb on - strand, within Pf6N2E2_5910 at 6729.782 kb on + strand at 6729.782 kb on + strand at 6729.782 kb on + strand at 6729.782 kb on + strand at 6729.783 kb on - strand at 6729.837 kb on + strand at 6729.837 kb on + strand at 6729.837 kb on + strand at 6729.837 kb on + strand at 6729.837 kb on + strand at 6729.837 kb on + strand at 6729.838 kb on - strand at 6729.838 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 6,726,687 - -0.5 6,726,700 + -0.5 6,726,727 + +0.7 6,726,778 + +0.3 6,726,778 + -0.2 6,726,784 - -2.1 6,726,829 + +2.3 6,726,829 + -0.1 6,726,829 + -1.3 6,726,829 + +0.3 6,726,829 + +0.6 6,726,830 - +0.6 6,727,172 + Pf6N2E2_5908 0.26 -0.6 6,727,172 + Pf6N2E2_5908 0.26 -0.9 6,727,173 - Pf6N2E2_5908 0.26 -0.7 6,727,173 - Pf6N2E2_5908 0.26 -2.1 6,727,173 - Pf6N2E2_5908 0.26 +0.2 6,727,173 - Pf6N2E2_5908 0.26 +0.1 6,727,173 - Pf6N2E2_5908 0.26 +0.1 6,727,174 + Pf6N2E2_5908 0.26 -0.6 6,727,175 - Pf6N2E2_5908 0.27 +0.2 6,727,175 - Pf6N2E2_5908 0.27 +2.5 6,727,206 + Pf6N2E2_5908 0.32 -2.1 6,727,207 - Pf6N2E2_5908 0.33 -0.4 6,727,207 - Pf6N2E2_5908 0.33 +0.0 6,727,243 + Pf6N2E2_5908 0.39 -0.9 6,727,244 - Pf6N2E2_5908 0.40 -0.4 6,727,244 - Pf6N2E2_5908 0.40 -0.2 6,727,247 + Pf6N2E2_5908 0.40 +0.1 6,727,247 + Pf6N2E2_5908 0.40 -0.7 6,727,247 + Pf6N2E2_5908 0.40 +0.9 6,727,247 + Pf6N2E2_5908 0.40 +0.1 6,727,247 + Pf6N2E2_5908 0.40 +1.7 6,727,248 - Pf6N2E2_5908 0.40 -0.0 6,727,248 - Pf6N2E2_5908 0.40 -0.3 6,727,248 - Pf6N2E2_5908 0.40 +1.7 6,727,248 - Pf6N2E2_5908 0.40 -0.4 6,727,320 - Pf6N2E2_5908 0.54 +0.6 6,727,445 + Pf6N2E2_5908 0.77 +0.2 6,727,446 - Pf6N2E2_5908 0.78 -0.5 6,727,446 - Pf6N2E2_5908 0.78 -1.8 6,727,562 + -1.8 6,727,563 - -1.6 6,727,563 - -1.6 6,727,563 - -1.5 6,727,563 - -0.4 6,727,838 + Pf6N2E2_5909 0.20 +0.3 6,727,838 + Pf6N2E2_5909 0.20 +1.3 6,727,839 - Pf6N2E2_5909 0.20 -0.1 6,727,909 + Pf6N2E2_5909 0.26 -0.3 6,727,910 - Pf6N2E2_5909 0.26 +0.7 6,727,942 + Pf6N2E2_5909 0.28 -1.1 6,727,942 + Pf6N2E2_5909 0.28 -1.6 6,727,942 + Pf6N2E2_5909 0.28 +0.1 6,727,943 - Pf6N2E2_5909 0.28 +0.1 6,728,089 + Pf6N2E2_5909 0.39 -1.0 6,728,089 + Pf6N2E2_5909 0.39 -0.1 6,728,089 + Pf6N2E2_5909 0.39 +1.7 6,728,089 + Pf6N2E2_5909 0.39 -1.5 6,728,090 - Pf6N2E2_5909 0.39 -0.2 6,728,090 - Pf6N2E2_5909 0.39 -0.1 6,728,090 - Pf6N2E2_5909 0.39 +0.1 6,728,090 - Pf6N2E2_5909 0.39 +0.1 6,728,090 - Pf6N2E2_5909 0.39 +0.1 6,728,090 - Pf6N2E2_5909 0.39 +0.1 6,728,116 + Pf6N2E2_5909 0.41 -0.7 6,728,116 + Pf6N2E2_5909 0.41 -0.3 6,728,116 + Pf6N2E2_5909 0.41 -3.2 6,728,117 - Pf6N2E2_5909 0.41 -0.5 6,728,117 - Pf6N2E2_5909 0.41 -1.2 6,728,117 - Pf6N2E2_5909 0.41 +0.1 6,728,203 + Pf6N2E2_5909 0.48 -1.3 6,728,204 - Pf6N2E2_5909 0.48 +0.3 6,728,204 - Pf6N2E2_5909 0.48 -0.9 6,728,353 + Pf6N2E2_5909 0.59 -1.0 6,728,374 + Pf6N2E2_5909 0.61 +1.7 6,728,617 + Pf6N2E2_5909 0.79 -0.1 6,728,618 - Pf6N2E2_5909 0.79 -0.3 6,728,618 - Pf6N2E2_5909 0.79 -0.1 6,728,654 - Pf6N2E2_5909 0.82 -0.5 6,728,698 + Pf6N2E2_5909 0.85 -3.4 6,728,698 + Pf6N2E2_5909 0.85 +0.7 6,728,719 + Pf6N2E2_5909 0.87 -1.1 6,728,720 - Pf6N2E2_5909 0.87 +0.7 6,728,720 - Pf6N2E2_5909 0.87 +0.7 6,728,720 - Pf6N2E2_5909 0.87 -2.0 6,728,720 - Pf6N2E2_5909 0.87 -0.8 6,728,720 - Pf6N2E2_5909 0.87 +1.0 6,728,737 + Pf6N2E2_5909 0.88 +0.7 6,728,737 + Pf6N2E2_5909 0.88 -0.5 6,728,737 + Pf6N2E2_5909 0.88 -0.9 6,728,737 + Pf6N2E2_5909 0.88 -0.3 6,728,737 + Pf6N2E2_5909 0.88 -0.6 6,728,737 + Pf6N2E2_5909 0.88 +0.2 6,728,738 - Pf6N2E2_5909 0.88 -0.1 6,728,738 - Pf6N2E2_5909 0.88 -0.1 6,728,738 - Pf6N2E2_5909 0.88 -1.3 6,728,738 - Pf6N2E2_5909 0.88 +0.8 6,728,812 + +0.1 6,728,869 + -0.8 6,728,869 + +1.7 6,728,869 + -1.9 6,728,869 + +1.7 6,728,869 + +1.7 6,728,869 + +1.0 6,728,870 - +1.7 6,728,870 - -0.6 6,728,870 - +0.7 6,728,870 - -0.3 6,728,922 + +0.1 6,728,922 + +1.7 6,728,923 - -0.3 6,729,156 - Pf6N2E2_5910 0.24 -1.7 6,729,644 + Pf6N2E2_5910 0.89 -0.3 6,729,645 - Pf6N2E2_5910 0.89 +0.2 6,729,782 + -1.0 6,729,782 + -0.7 6,729,782 + -0.9 6,729,782 + -2.6 6,729,783 - +1.7 6,729,837 + +0.7 6,729,837 + -0.4 6,729,837 + +0.7 6,729,837 + -1.0 6,729,837 + -0.8 6,729,837 + -0.1 6,729,838 - -0.5 6,729,838 - -0.5
Or see this region's nucleotide sequence