Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_490 and Pf6N2E2_491 are separated by 788 nucleotides Pf6N2E2_491 and Pf6N2E2_492 are separated by 273 nucleotides Pf6N2E2_492 and Pf6N2E2_493 are separated by 55 nucleotides
Pf6N2E2_490 - PhaK-like protein, at 556,392 to 557,693
_490
Pf6N2E2_491 - hypothetical protein, at 558,482 to 558,835
_491
Pf6N2E2_492 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 559,109 to 559,729
_492
Pf6N2E2_493 - YD repeat protein, at 559,785 to 560,831
_493
Position (kb) 558
559 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 557.538 kb on - strand, within Pf6N2E2_490 at 557.694 kb on + strand at 557.694 kb on + strand at 557.759 kb on + strand at 557.803 kb on + strand at 557.803 kb on + strand at 557.949 kb on + strand at 557.968 kb on + strand at 557.968 kb on + strand at 558.118 kb on - strand at 558.154 kb on + strand at 558.154 kb on + strand at 558.154 kb on + strand at 558.154 kb on + strand at 558.155 kb on - strand at 558.155 kb on - strand at 558.175 kb on + strand at 558.175 kb on + strand at 558.176 kb on - strand at 558.176 kb on - strand at 558.176 kb on - strand at 558.176 kb on - strand at 558.197 kb on + strand at 558.197 kb on + strand at 558.198 kb on - strand at 558.198 kb on - strand at 558.198 kb on - strand at 558.231 kb on + strand at 558.232 kb on - strand at 558.245 kb on + strand at 558.246 kb on - strand at 558.246 kb on - strand at 558.306 kb on - strand at 558.397 kb on + strand at 558.397 kb on + strand at 558.409 kb on + strand at 558.410 kb on - strand at 558.423 kb on + strand at 558.424 kb on - strand at 558.424 kb on - strand at 558.458 kb on + strand at 558.458 kb on + strand at 558.458 kb on + strand at 558.459 kb on - strand at 558.459 kb on - strand at 558.459 kb on - strand at 558.461 kb on + strand at 558.461 kb on + strand at 558.461 kb on + strand at 558.462 kb on - strand at 558.462 kb on - strand at 558.462 kb on - strand at 558.462 kb on - strand at 558.462 kb on - strand at 558.481 kb on + strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.578 kb on + strand, within Pf6N2E2_491 at 558.578 kb on + strand, within Pf6N2E2_491 at 558.579 kb on - strand, within Pf6N2E2_491 at 558.579 kb on - strand, within Pf6N2E2_491 at 558.579 kb on - strand, within Pf6N2E2_491 at 558.581 kb on + strand, within Pf6N2E2_491 at 558.581 kb on + strand, within Pf6N2E2_491 at 558.582 kb on - strand, within Pf6N2E2_491 at 558.582 kb on - strand, within Pf6N2E2_491 at 558.582 kb on - strand, within Pf6N2E2_491 at 558.796 kb on - strand, within Pf6N2E2_491 at 558.870 kb on - strand at 558.870 kb on - strand at 558.900 kb on - strand at 558.998 kb on + strand at 558.998 kb on + strand at 558.998 kb on + strand at 558.999 kb on - strand at 558.999 kb on - strand at 558.999 kb on - strand at 558.999 kb on - strand at 558.999 kb on - strand at 559.018 kb on + strand at 559.018 kb on + strand at 559.019 kb on - strand at 559.141 kb on + strand at 559.142 kb on - strand at 559.256 kb on + strand, within Pf6N2E2_492 at 559.256 kb on + strand, within Pf6N2E2_492 at 559.257 kb on - strand, within Pf6N2E2_492 at 559.257 kb on - strand, within Pf6N2E2_492 at 559.259 kb on + strand, within Pf6N2E2_492 at 559.259 kb on + strand, within Pf6N2E2_492 at 559.260 kb on - strand, within Pf6N2E2_492 at 559.260 kb on - strand, within Pf6N2E2_492 at 559.260 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.284 kb on - strand, within Pf6N2E2_492 at 559.750 kb on + strand at 559.751 kb on - strand at 559.751 kb on - strand at 559.758 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 557,538 - Pf6N2E2_490 0.88 -0.0 557,694 + -1.0 557,694 + +1.6 557,759 + -1.4 557,803 + -0.1 557,803 + -0.8 557,949 + -0.2 557,968 + -0.3 557,968 + +2.4 558,118 - -1.2 558,154 + -0.3 558,154 + -2.2 558,154 + -0.7 558,154 + +1.6 558,155 - -1.0 558,155 - -0.0 558,175 + +1.6 558,175 + -0.9 558,176 - -0.1 558,176 - +0.1 558,176 - +0.6 558,176 - -0.4 558,197 + -1.3 558,197 + +1.6 558,198 - +1.6 558,198 - +1.6 558,198 - -2.1 558,231 + -0.5 558,232 - -0.0 558,245 + +3.2 558,246 - -0.4 558,246 - +0.6 558,306 - +0.3 558,397 + -0.0 558,397 + +1.9 558,409 + -0.0 558,410 - +1.6 558,423 + +0.6 558,424 - +0.1 558,424 - +1.6 558,458 + -0.8 558,458 + +0.9 558,458 + +0.4 558,459 - -0.9 558,459 - +0.6 558,459 - +0.4 558,461 + +1.3 558,461 + -0.7 558,461 + +1.6 558,462 - -0.5 558,462 - -0.4 558,462 - -0.9 558,462 - -0.1 558,462 - -0.2 558,481 + +0.6 558,482 - +1.6 558,482 - +0.4 558,482 - -0.9 558,482 - -0.1 558,482 - -1.0 558,482 - +0.6 558,482 - -0.7 558,482 - +1.9 558,482 - -1.2 558,482 - -0.6 558,482 - -0.0 558,578 + Pf6N2E2_491 0.27 +1.6 558,578 + Pf6N2E2_491 0.27 +1.4 558,579 - Pf6N2E2_491 0.27 +0.8 558,579 - Pf6N2E2_491 0.27 +0.4 558,579 - Pf6N2E2_491 0.27 -0.4 558,581 + Pf6N2E2_491 0.28 +0.1 558,581 + Pf6N2E2_491 0.28 -0.4 558,582 - Pf6N2E2_491 0.28 +0.6 558,582 - Pf6N2E2_491 0.28 +0.7 558,582 - Pf6N2E2_491 0.28 +1.8 558,796 - Pf6N2E2_491 0.89 +0.3 558,870 - +0.6 558,870 - -1.8 558,900 - -2.2 558,998 + +1.6 558,998 + -1.2 558,998 + -0.3 558,999 - -1.7 558,999 - -1.9 558,999 - +0.6 558,999 - -0.0 558,999 - -0.3 559,018 + +0.3 559,018 + +0.2 559,019 - +1.1 559,141 + -0.0 559,142 - -0.4 559,256 + Pf6N2E2_492 0.24 -1.4 559,256 + Pf6N2E2_492 0.24 -0.4 559,257 - Pf6N2E2_492 0.24 -0.4 559,257 - Pf6N2E2_492 0.24 +0.3 559,259 + Pf6N2E2_492 0.24 -0.2 559,259 + Pf6N2E2_492 0.24 +1.6 559,260 - Pf6N2E2_492 0.24 -0.5 559,260 - Pf6N2E2_492 0.24 -1.6 559,260 - Pf6N2E2_492 0.24 -1.1 559,284 - Pf6N2E2_492 0.28 +1.6 559,284 - Pf6N2E2_492 0.28 +1.6 559,284 - Pf6N2E2_492 0.28 -1.3 559,284 - Pf6N2E2_492 0.28 +1.6 559,284 - Pf6N2E2_492 0.28 -0.1 559,750 + -0.3 559,751 - +0.6 559,751 - -0.3 559,758 - +0.8
Or see this region's nucleotide sequence