Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4357 and Pf6N2E2_4358 are separated by 248 nucleotides Pf6N2E2_4358 and Pf6N2E2_4359 are separated by 51 nucleotides Pf6N2E2_4359 and Pf6N2E2_4360 are separated by 55 nucleotides
Pf6N2E2_4357 - Nucleoside-diphosphate-sugar epimerases, at 5,087,694 to 5,088,551
_4357
Pf6N2E2_4358 - Permease of the drug/metabolite transporter (DMT) superfamily, at 5,088,800 to 5,089,699
_4358
Pf6N2E2_4359 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,089,751 to 5,090,491
_4359
Pf6N2E2_4360 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 5,090,547 to 5,090,927
_4360
Position (kb)
5088
5089
5090 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5087.889 kb on + strand, within Pf6N2E2_4357 at 5087.890 kb on - strand, within Pf6N2E2_4357 at 5087.890 kb on - strand, within Pf6N2E2_4357 at 5087.898 kb on + strand, within Pf6N2E2_4357 at 5087.898 kb on + strand, within Pf6N2E2_4357 at 5087.898 kb on + strand, within Pf6N2E2_4357 at 5087.899 kb on - strand, within Pf6N2E2_4357 at 5087.899 kb on - strand, within Pf6N2E2_4357 at 5087.946 kb on + strand, within Pf6N2E2_4357 at 5087.946 kb on + strand, within Pf6N2E2_4357 at 5087.946 kb on + strand, within Pf6N2E2_4357 at 5087.946 kb on + strand, within Pf6N2E2_4357 at 5087.946 kb on + strand, within Pf6N2E2_4357 at 5087.947 kb on - strand, within Pf6N2E2_4357 at 5087.947 kb on - strand, within Pf6N2E2_4357 at 5088.094 kb on - strand, within Pf6N2E2_4357 at 5088.177 kb on + strand, within Pf6N2E2_4357 at 5088.177 kb on + strand, within Pf6N2E2_4357 at 5088.177 kb on + strand, within Pf6N2E2_4357 at 5088.177 kb on + strand, within Pf6N2E2_4357 at 5088.177 kb on + strand, within Pf6N2E2_4357 at 5088.177 kb on + strand, within Pf6N2E2_4357 at 5088.177 kb on + strand, within Pf6N2E2_4357 at 5088.178 kb on - strand, within Pf6N2E2_4357 at 5088.178 kb on - strand, within Pf6N2E2_4357 at 5088.178 kb on - strand, within Pf6N2E2_4357 at 5088.178 kb on - strand, within Pf6N2E2_4357 at 5088.178 kb on - strand, within Pf6N2E2_4357 at 5088.178 kb on - strand, within Pf6N2E2_4357 at 5088.178 kb on - strand, within Pf6N2E2_4357 at 5088.178 kb on - strand, within Pf6N2E2_4357 at 5088.249 kb on + strand, within Pf6N2E2_4357 at 5088.250 kb on - strand, within Pf6N2E2_4357 at 5088.250 kb on - strand, within Pf6N2E2_4357 at 5088.339 kb on + strand, within Pf6N2E2_4357 at 5088.339 kb on + strand, within Pf6N2E2_4357 at 5088.339 kb on + strand, within Pf6N2E2_4357 at 5088.339 kb on + strand, within Pf6N2E2_4357 at 5088.339 kb on + strand, within Pf6N2E2_4357 at 5088.340 kb on - strand, within Pf6N2E2_4357 at 5088.340 kb on - strand, within Pf6N2E2_4357 at 5088.399 kb on + strand, within Pf6N2E2_4357 at 5088.399 kb on + strand, within Pf6N2E2_4357 at 5088.399 kb on + strand, within Pf6N2E2_4357 at 5088.399 kb on + strand, within Pf6N2E2_4357 at 5088.399 kb on + strand, within Pf6N2E2_4357 at 5088.399 kb on + strand, within Pf6N2E2_4357 at 5088.399 kb on + strand, within Pf6N2E2_4357 at 5088.399 kb on + strand, within Pf6N2E2_4357 at 5088.400 kb on - strand, within Pf6N2E2_4357 at 5088.400 kb on - strand, within Pf6N2E2_4357 at 5088.400 kb on - strand, within Pf6N2E2_4357 at 5088.400 kb on - strand, within Pf6N2E2_4357 at 5088.400 kb on - strand, within Pf6N2E2_4357 at 5088.400 kb on - strand, within Pf6N2E2_4357 at 5088.522 kb on + strand at 5088.581 kb on + strand at 5088.581 kb on + strand at 5088.581 kb on + strand at 5088.581 kb on + strand at 5088.582 kb on - strand at 5088.582 kb on - strand at 5088.582 kb on - strand at 5088.582 kb on - strand at 5088.582 kb on - strand at 5088.601 kb on + strand at 5088.601 kb on + strand at 5088.601 kb on + strand at 5088.602 kb on - strand at 5088.602 kb on - strand at 5088.610 kb on - strand at 5088.619 kb on + strand at 5088.620 kb on - strand at 5088.620 kb on - strand at 5088.631 kb on + strand at 5088.631 kb on + strand at 5088.631 kb on + strand at 5088.632 kb on - strand at 5088.777 kb on - strand at 5088.777 kb on - strand at 5088.777 kb on - strand at 5088.853 kb on + strand at 5088.853 kb on + strand at 5088.854 kb on - strand at 5088.854 kb on - strand at 5088.854 kb on - strand at 5088.924 kb on + strand, within Pf6N2E2_4358 at 5088.924 kb on + strand, within Pf6N2E2_4358 at 5088.945 kb on + strand, within Pf6N2E2_4358 at 5088.945 kb on + strand, within Pf6N2E2_4358 at 5088.945 kb on + strand, within Pf6N2E2_4358 at 5088.945 kb on + strand, within Pf6N2E2_4358 at 5088.945 kb on + strand, within Pf6N2E2_4358 at 5088.945 kb on + strand, within Pf6N2E2_4358 at 5088.946 kb on - strand, within Pf6N2E2_4358 at 5088.946 kb on - strand, within Pf6N2E2_4358 at 5088.946 kb on - strand, within Pf6N2E2_4358 at 5088.946 kb on - strand, within Pf6N2E2_4358 at 5088.970 kb on + strand, within Pf6N2E2_4358 at 5088.970 kb on + strand, within Pf6N2E2_4358 at 5089.030 kb on + strand, within Pf6N2E2_4358 at 5089.030 kb on + strand, within Pf6N2E2_4358 at 5089.073 kb on + strand, within Pf6N2E2_4358 at 5089.074 kb on - strand, within Pf6N2E2_4358 at 5089.089 kb on + strand, within Pf6N2E2_4358 at 5089.089 kb on + strand, within Pf6N2E2_4358 at 5089.089 kb on + strand, within Pf6N2E2_4358 at 5089.089 kb on + strand, within Pf6N2E2_4358 at 5089.089 kb on + strand, within Pf6N2E2_4358 at 5089.090 kb on - strand, within Pf6N2E2_4358 at 5089.143 kb on + strand, within Pf6N2E2_4358 at 5089.143 kb on + strand, within Pf6N2E2_4358 at 5089.144 kb on - strand, within Pf6N2E2_4358 at 5089.144 kb on - strand, within Pf6N2E2_4358 at 5089.144 kb on - strand, within Pf6N2E2_4358 at 5089.144 kb on - strand, within Pf6N2E2_4358 at 5089.373 kb on + strand, within Pf6N2E2_4358 at 5089.373 kb on + strand, within Pf6N2E2_4358 at 5089.373 kb on + strand, within Pf6N2E2_4358 at 5089.373 kb on + strand, within Pf6N2E2_4358 at 5089.373 kb on + strand, within Pf6N2E2_4358 at 5089.374 kb on - strand, within Pf6N2E2_4358 at 5089.374 kb on - strand, within Pf6N2E2_4358 at 5089.505 kb on + strand, within Pf6N2E2_4358 at 5089.506 kb on - strand, within Pf6N2E2_4358 at 5089.724 kb on + strand at 5089.725 kb on - strand at 5089.730 kb on + strand at 5089.730 kb on + strand at 5089.731 kb on - strand at 5089.731 kb on - strand at 5089.731 kb on - strand at 5090.067 kb on + strand, within Pf6N2E2_4359 at 5090.171 kb on + strand, within Pf6N2E2_4359 at 5090.172 kb on - strand, within Pf6N2E2_4359 at 5090.214 kb on + strand, within Pf6N2E2_4359 at 5090.286 kb on + strand, within Pf6N2E2_4359 at 5090.545 kb on + strand at 5090.545 kb on + strand at 5090.545 kb on + strand at 5090.546 kb on - strand at 5090.546 kb on - strand at 5090.548 kb on + strand at 5090.548 kb on + strand at 5090.549 kb on - strand at 5090.549 kb on - strand at 5090.549 kb on - strand at 5090.629 kb on + strand, within Pf6N2E2_4360 at 5090.629 kb on + strand, within Pf6N2E2_4360 at 5090.644 kb on + strand, within Pf6N2E2_4360 at 5090.644 kb on + strand, within Pf6N2E2_4360 at 5090.644 kb on + strand, within Pf6N2E2_4360 at 5090.644 kb on + strand, within Pf6N2E2_4360 at 5090.644 kb on + strand, within Pf6N2E2_4360 at 5090.645 kb on - strand, within Pf6N2E2_4360 at 5090.645 kb on - strand, within Pf6N2E2_4360 at 5090.645 kb on - strand, within Pf6N2E2_4360 at 5090.645 kb on - strand, within Pf6N2E2_4360
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 5,087,889 + Pf6N2E2_4357 0.23 +0.5 5,087,890 - Pf6N2E2_4357 0.23 -1.4 5,087,890 - Pf6N2E2_4357 0.23 +0.1 5,087,898 + Pf6N2E2_4357 0.24 -1.0 5,087,898 + Pf6N2E2_4357 0.24 +0.3 5,087,898 + Pf6N2E2_4357 0.24 +0.8 5,087,899 - Pf6N2E2_4357 0.24 +0.7 5,087,899 - Pf6N2E2_4357 0.24 -0.6 5,087,946 + Pf6N2E2_4357 0.29 -0.3 5,087,946 + Pf6N2E2_4357 0.29 -0.3 5,087,946 + Pf6N2E2_4357 0.29 -2.0 5,087,946 + Pf6N2E2_4357 0.29 +0.4 5,087,946 + Pf6N2E2_4357 0.29 +0.1 5,087,947 - Pf6N2E2_4357 0.29 -0.3 5,087,947 - Pf6N2E2_4357 0.29 -1.8 5,088,094 - Pf6N2E2_4357 0.47 -1.3 5,088,177 + Pf6N2E2_4357 0.56 +0.5 5,088,177 + Pf6N2E2_4357 0.56 +0.2 5,088,177 + Pf6N2E2_4357 0.56 -0.3 5,088,177 + Pf6N2E2_4357 0.56 +0.1 5,088,177 + Pf6N2E2_4357 0.56 -1.3 5,088,177 + Pf6N2E2_4357 0.56 -0.8 5,088,177 + Pf6N2E2_4357 0.56 +0.2 5,088,178 - Pf6N2E2_4357 0.56 -0.3 5,088,178 - Pf6N2E2_4357 0.56 -1.1 5,088,178 - Pf6N2E2_4357 0.56 -0.3 5,088,178 - Pf6N2E2_4357 0.56 -0.1 5,088,178 - Pf6N2E2_4357 0.56 -1.6 5,088,178 - Pf6N2E2_4357 0.56 +0.6 5,088,178 - Pf6N2E2_4357 0.56 -1.8 5,088,178 - Pf6N2E2_4357 0.56 -0.0 5,088,249 + Pf6N2E2_4357 0.65 -0.9 5,088,250 - Pf6N2E2_4357 0.65 +0.6 5,088,250 - Pf6N2E2_4357 0.65 -2.3 5,088,339 + Pf6N2E2_4357 0.75 -0.1 5,088,339 + Pf6N2E2_4357 0.75 -2.0 5,088,339 + Pf6N2E2_4357 0.75 -2.3 5,088,339 + Pf6N2E2_4357 0.75 +0.7 5,088,339 + Pf6N2E2_4357 0.75 +1.7 5,088,340 - Pf6N2E2_4357 0.75 -0.3 5,088,340 - Pf6N2E2_4357 0.75 -0.8 5,088,399 + Pf6N2E2_4357 0.82 +1.0 5,088,399 + Pf6N2E2_4357 0.82 -0.5 5,088,399 + Pf6N2E2_4357 0.82 -1.5 5,088,399 + Pf6N2E2_4357 0.82 +1.0 5,088,399 + Pf6N2E2_4357 0.82 -2.0 5,088,399 + Pf6N2E2_4357 0.82 -1.8 5,088,399 + Pf6N2E2_4357 0.82 -0.4 5,088,399 + Pf6N2E2_4357 0.82 +0.4 5,088,400 - Pf6N2E2_4357 0.82 +0.1 5,088,400 - Pf6N2E2_4357 0.82 -1.0 5,088,400 - Pf6N2E2_4357 0.82 -1.1 5,088,400 - Pf6N2E2_4357 0.82 -1.5 5,088,400 - Pf6N2E2_4357 0.82 -0.6 5,088,400 - Pf6N2E2_4357 0.82 -1.6 5,088,522 + -1.6 5,088,581 + -1.7 5,088,581 + -2.1 5,088,581 + -0.3 5,088,581 + -0.7 5,088,582 - +0.4 5,088,582 - +0.6 5,088,582 - +0.1 5,088,582 - +0.6 5,088,582 - +0.9 5,088,601 + -1.1 5,088,601 + -0.3 5,088,601 + +1.7 5,088,602 - +0.0 5,088,602 - +1.0 5,088,610 - -1.3 5,088,619 + +0.3 5,088,620 - -1.5 5,088,620 - +1.7 5,088,631 + +1.7 5,088,631 + -1.3 5,088,631 + -0.2 5,088,632 - -0.2 5,088,777 - +0.3 5,088,777 - +0.8 5,088,777 - -0.6 5,088,853 + +0.8 5,088,853 + +0.1 5,088,854 - -0.2 5,088,854 - -1.2 5,088,854 - +0.3 5,088,924 + Pf6N2E2_4358 0.14 +1.7 5,088,924 + Pf6N2E2_4358 0.14 -0.1 5,088,945 + Pf6N2E2_4358 0.16 +0.4 5,088,945 + Pf6N2E2_4358 0.16 -1.3 5,088,945 + Pf6N2E2_4358 0.16 -0.0 5,088,945 + Pf6N2E2_4358 0.16 -0.6 5,088,945 + Pf6N2E2_4358 0.16 -1.9 5,088,945 + Pf6N2E2_4358 0.16 -1.4 5,088,946 - Pf6N2E2_4358 0.16 -0.6 5,088,946 - Pf6N2E2_4358 0.16 +1.7 5,088,946 - Pf6N2E2_4358 0.16 +0.1 5,088,946 - Pf6N2E2_4358 0.16 +0.4 5,088,970 + Pf6N2E2_4358 0.19 +0.7 5,088,970 + Pf6N2E2_4358 0.19 -1.7 5,089,030 + Pf6N2E2_4358 0.26 -0.8 5,089,030 + Pf6N2E2_4358 0.26 +0.3 5,089,073 + Pf6N2E2_4358 0.30 -1.8 5,089,074 - Pf6N2E2_4358 0.30 +0.4 5,089,089 + Pf6N2E2_4358 0.32 +0.7 5,089,089 + Pf6N2E2_4358 0.32 +0.1 5,089,089 + Pf6N2E2_4358 0.32 -0.6 5,089,089 + Pf6N2E2_4358 0.32 -0.3 5,089,089 + Pf6N2E2_4358 0.32 -0.6 5,089,090 - Pf6N2E2_4358 0.32 +0.1 5,089,143 + Pf6N2E2_4358 0.38 -1.0 5,089,143 + Pf6N2E2_4358 0.38 -0.5 5,089,144 - Pf6N2E2_4358 0.38 -0.3 5,089,144 - Pf6N2E2_4358 0.38 +0.1 5,089,144 - Pf6N2E2_4358 0.38 -0.9 5,089,144 - Pf6N2E2_4358 0.38 -3.4 5,089,373 + Pf6N2E2_4358 0.64 +0.6 5,089,373 + Pf6N2E2_4358 0.64 -0.7 5,089,373 + Pf6N2E2_4358 0.64 +1.2 5,089,373 + Pf6N2E2_4358 0.64 +0.1 5,089,373 + Pf6N2E2_4358 0.64 -1.2 5,089,374 - Pf6N2E2_4358 0.64 -0.1 5,089,374 - Pf6N2E2_4358 0.64 +0.6 5,089,505 + Pf6N2E2_4358 0.78 -2.8 5,089,506 - Pf6N2E2_4358 0.78 +2.3 5,089,724 + -0.0 5,089,725 - -0.7 5,089,730 + +0.4 5,089,730 + +0.3 5,089,731 - +0.3 5,089,731 - +1.7 5,089,731 - +0.1 5,090,067 + Pf6N2E2_4359 0.43 +0.7 5,090,171 + Pf6N2E2_4359 0.57 +0.1 5,090,172 - Pf6N2E2_4359 0.57 +1.0 5,090,214 + Pf6N2E2_4359 0.62 -0.6 5,090,286 + Pf6N2E2_4359 0.72 -0.2 5,090,545 + -0.0 5,090,545 + -0.6 5,090,545 + +0.2 5,090,546 - +0.3 5,090,546 - +1.7 5,090,548 + +0.7 5,090,548 + +0.7 5,090,549 - -0.5 5,090,549 - -1.6 5,090,549 - +0.1 5,090,629 + Pf6N2E2_4360 0.22 -1.1 5,090,629 + Pf6N2E2_4360 0.22 -0.3 5,090,644 + Pf6N2E2_4360 0.25 -1.9 5,090,644 + Pf6N2E2_4360 0.25 +0.0 5,090,644 + Pf6N2E2_4360 0.25 +0.4 5,090,644 + Pf6N2E2_4360 0.25 -1.2 5,090,644 + Pf6N2E2_4360 0.25 -0.1 5,090,645 - Pf6N2E2_4360 0.26 -0.3 5,090,645 - Pf6N2E2_4360 0.26 -1.6 5,090,645 - Pf6N2E2_4360 0.26 -0.2 5,090,645 - Pf6N2E2_4360 0.26 +1.7
Or see this region's nucleotide sequence