Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3726 and Pf6N2E2_3727 overlap by 4 nucleotides Pf6N2E2_3727 and Pf6N2E2_3728 overlap by 4 nucleotides Pf6N2E2_3728 and Pf6N2E2_3729 overlap by 1 nucleotides
Pf6N2E2_3726 - 3-oxoacyl-[ACP] synthase (EC 2.3.1.41) FabV like, at 4,339,742 to 4,340,938
_3726
Pf6N2E2_3727 - 3-hydroxydecanoyl-[ACP] dehydratase (EC 4.2.1.60), at 4,340,935 to 4,341,402
_3727
Pf6N2E2_3728 - 3-oxoacyl-[ACP] reductase (EC 1.1.1.100), at 4,341,399 to 4,342,127
_3728
Pf6N2E2_3729 - FIG138576: 3-oxoacyl-[ACP] synthase (EC 2.3.1.41), at 4,342,127 to 4,343,353
_3729
Position (kb)
4341
4342
4343 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4340.491 kb on + strand, within Pf6N2E2_3726 at 4340.491 kb on + strand, within Pf6N2E2_3726 at 4340.492 kb on - strand, within Pf6N2E2_3726 at 4340.583 kb on - strand, within Pf6N2E2_3726 at 4340.761 kb on + strand, within Pf6N2E2_3726 at 4341.338 kb on + strand, within Pf6N2E2_3727 at 4341.339 kb on - strand, within Pf6N2E2_3727 at 4341.339 kb on - strand, within Pf6N2E2_3727 at 4341.364 kb on + strand at 4341.639 kb on + strand, within Pf6N2E2_3728 at 4341.639 kb on + strand, within Pf6N2E2_3728 at 4341.640 kb on - strand, within Pf6N2E2_3728 at 4341.640 kb on - strand, within Pf6N2E2_3728 at 4341.640 kb on - strand, within Pf6N2E2_3728 at 4341.643 kb on - strand, within Pf6N2E2_3728 at 4341.744 kb on + strand, within Pf6N2E2_3728 at 4341.745 kb on - strand, within Pf6N2E2_3728 at 4341.745 kb on - strand, within Pf6N2E2_3728 at 4341.855 kb on + strand, within Pf6N2E2_3728 at 4341.855 kb on + strand, within Pf6N2E2_3728 at 4341.855 kb on + strand, within Pf6N2E2_3728 at 4341.855 kb on + strand, within Pf6N2E2_3728 at 4341.855 kb on + strand, within Pf6N2E2_3728 at 4341.856 kb on - strand, within Pf6N2E2_3728 at 4341.856 kb on - strand, within Pf6N2E2_3728 at 4341.856 kb on - strand, within Pf6N2E2_3728 at 4341.856 kb on - strand, within Pf6N2E2_3728 at 4341.856 kb on - strand, within Pf6N2E2_3728 at 4341.856 kb on - strand, within Pf6N2E2_3728 at 4342.083 kb on + strand at 4342.084 kb on - strand at 4342.529 kb on + strand, within Pf6N2E2_3729 at 4342.649 kb on + strand, within Pf6N2E2_3729 at 4342.650 kb on - strand, within Pf6N2E2_3729 at 4342.742 kb on + strand, within Pf6N2E2_3729 at 4343.016 kb on - strand, within Pf6N2E2_3729 at 4343.016 kb on - strand, within Pf6N2E2_3729 at 4343.016 kb on - strand, within Pf6N2E2_3729
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 4,340,491 + Pf6N2E2_3726 0.63 +0.4 4,340,491 + Pf6N2E2_3726 0.63 -0.6 4,340,492 - Pf6N2E2_3726 0.63 +0.2 4,340,583 - Pf6N2E2_3726 0.70 +1.4 4,340,761 + Pf6N2E2_3726 0.85 +0.7 4,341,338 + Pf6N2E2_3727 0.86 +0.5 4,341,339 - Pf6N2E2_3727 0.86 -2.5 4,341,339 - Pf6N2E2_3727 0.86 -0.2 4,341,364 + +0.3 4,341,639 + Pf6N2E2_3728 0.33 -2.3 4,341,639 + Pf6N2E2_3728 0.33 +0.5 4,341,640 - Pf6N2E2_3728 0.33 +1.3 4,341,640 - Pf6N2E2_3728 0.33 +0.2 4,341,640 - Pf6N2E2_3728 0.33 +3.3 4,341,643 - Pf6N2E2_3728 0.33 +0.1 4,341,744 + Pf6N2E2_3728 0.47 +0.1 4,341,745 - Pf6N2E2_3728 0.47 -1.0 4,341,745 - Pf6N2E2_3728 0.47 -0.9 4,341,855 + Pf6N2E2_3728 0.63 +0.5 4,341,855 + Pf6N2E2_3728 0.63 +0.1 4,341,855 + Pf6N2E2_3728 0.63 -1.1 4,341,855 + Pf6N2E2_3728 0.63 +0.8 4,341,855 + Pf6N2E2_3728 0.63 +1.2 4,341,856 - Pf6N2E2_3728 0.63 -0.8 4,341,856 - Pf6N2E2_3728 0.63 +0.4 4,341,856 - Pf6N2E2_3728 0.63 +0.1 4,341,856 - Pf6N2E2_3728 0.63 +1.0 4,341,856 - Pf6N2E2_3728 0.63 +0.9 4,341,856 - Pf6N2E2_3728 0.63 -0.3 4,342,083 + -2.4 4,342,084 - +0.2 4,342,529 + Pf6N2E2_3729 0.33 +1.7 4,342,649 + Pf6N2E2_3729 0.43 -0.3 4,342,650 - Pf6N2E2_3729 0.43 -1.2 4,342,742 + Pf6N2E2_3729 0.50 -0.3 4,343,016 - Pf6N2E2_3729 0.72 -0.8 4,343,016 - Pf6N2E2_3729 0.72 -0.6 4,343,016 - Pf6N2E2_3729 0.72 -0.6
Or see this region's nucleotide sequence