Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3221 and Pf6N2E2_3222 are separated by 108 nucleotides Pf6N2E2_3222 and Pf6N2E2_3223 are separated by 29 nucleotides Pf6N2E2_3223 and Pf6N2E2_3224 are separated by 129 nucleotides Pf6N2E2_3224 and Pf6N2E2_3225 are separated by 181 nucleotides
Pf6N2E2_3221 - FIG00352444: hypothetical protein, at 3,727,429 to 3,727,974
_3221
Pf6N2E2_3222 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 3,728,083 to 3,728,844
_3222
Pf6N2E2_3223 - uncharacterized protein SCO0976, at 3,728,874 to 3,729,506
_3223
Pf6N2E2_3224 - Lactoylglutathione lyase and related lyases, at 3,729,636 to 3,730,052
_3224
Pf6N2E2_3225 - hypothetical protein, at 3,730,234 to 3,731,283
_3225
Position (kb)
3728
3729
3730 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3728.027 kb on + strand at 3728.027 kb on + strand at 3728.031 kb on + strand at 3728.031 kb on + strand at 3728.031 kb on + strand at 3728.031 kb on + strand at 3728.031 kb on + strand at 3728.032 kb on - strand at 3728.032 kb on - strand at 3728.032 kb on - strand at 3728.036 kb on + strand at 3728.043 kb on - strand at 3728.125 kb on - strand at 3728.273 kb on + strand, within Pf6N2E2_3222 at 3728.273 kb on + strand, within Pf6N2E2_3222 at 3728.325 kb on - strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.396 kb on + strand, within Pf6N2E2_3222 at 3728.397 kb on - strand, within Pf6N2E2_3222 at 3728.397 kb on - strand, within Pf6N2E2_3222 at 3728.397 kb on - strand, within Pf6N2E2_3222 at 3728.397 kb on - strand, within Pf6N2E2_3222 at 3728.397 kb on - strand, within Pf6N2E2_3222 at 3728.397 kb on - strand, within Pf6N2E2_3222 at 3728.397 kb on - strand, within Pf6N2E2_3222 at 3728.397 kb on - strand, within Pf6N2E2_3222 at 3728.399 kb on + strand, within Pf6N2E2_3222 at 3728.399 kb on + strand, within Pf6N2E2_3222 at 3728.399 kb on + strand, within Pf6N2E2_3222 at 3728.399 kb on + strand, within Pf6N2E2_3222 at 3728.399 kb on + strand, within Pf6N2E2_3222 at 3728.399 kb on + strand, within Pf6N2E2_3222 at 3728.400 kb on - strand, within Pf6N2E2_3222 at 3728.400 kb on - strand, within Pf6N2E2_3222 at 3728.462 kb on + strand, within Pf6N2E2_3222 at 3728.462 kb on + strand, within Pf6N2E2_3222 at 3728.474 kb on + strand, within Pf6N2E2_3222 at 3728.493 kb on - strand, within Pf6N2E2_3222 at 3728.496 kb on + strand, within Pf6N2E2_3222 at 3728.497 kb on - strand, within Pf6N2E2_3222 at 3728.497 kb on - strand, within Pf6N2E2_3222 at 3728.676 kb on + strand, within Pf6N2E2_3222 at 3728.676 kb on + strand, within Pf6N2E2_3222 at 3728.676 kb on + strand, within Pf6N2E2_3222 at 3728.677 kb on - strand, within Pf6N2E2_3222 at 3728.677 kb on - strand, within Pf6N2E2_3222 at 3728.677 kb on - strand, within Pf6N2E2_3222 at 3728.677 kb on - strand, within Pf6N2E2_3222 at 3728.720 kb on + strand, within Pf6N2E2_3222 at 3728.720 kb on + strand, within Pf6N2E2_3222 at 3728.779 kb on + strand at 3728.837 kb on + strand at 3728.876 kb on - strand at 3728.876 kb on - strand at 3729.091 kb on + strand, within Pf6N2E2_3223 at 3729.091 kb on + strand, within Pf6N2E2_3223 at 3729.091 kb on + strand, within Pf6N2E2_3223 at 3729.092 kb on - strand, within Pf6N2E2_3223 at 3729.092 kb on - strand, within Pf6N2E2_3223 at 3729.092 kb on - strand, within Pf6N2E2_3223 at 3729.092 kb on - strand, within Pf6N2E2_3223 at 3729.092 kb on - strand, within Pf6N2E2_3223 at 3729.158 kb on + strand, within Pf6N2E2_3223 at 3729.158 kb on + strand, within Pf6N2E2_3223 at 3729.158 kb on + strand, within Pf6N2E2_3223 at 3729.159 kb on - strand, within Pf6N2E2_3223 at 3729.159 kb on - strand, within Pf6N2E2_3223 at 3729.159 kb on - strand, within Pf6N2E2_3223 at 3729.302 kb on + strand, within Pf6N2E2_3223 at 3729.303 kb on - strand, within Pf6N2E2_3223 at 3729.330 kb on + strand, within Pf6N2E2_3223 at 3729.331 kb on - strand, within Pf6N2E2_3223 at 3729.331 kb on - strand, within Pf6N2E2_3223 at 3729.338 kb on - strand, within Pf6N2E2_3223 at 3729.507 kb on - strand at 3729.531 kb on + strand at 3729.531 kb on + strand at 3729.531 kb on + strand at 3729.532 kb on - strand at 3729.532 kb on - strand at 3729.532 kb on - strand at 3729.603 kb on + strand at 3729.604 kb on - strand at 3729.635 kb on + strand at 3729.659 kb on + strand at 3729.660 kb on - strand at 3729.660 kb on - strand at 3729.683 kb on - strand, within Pf6N2E2_3224 at 3729.712 kb on - strand, within Pf6N2E2_3224 at 3729.712 kb on - strand, within Pf6N2E2_3224 at 3729.713 kb on + strand, within Pf6N2E2_3224 at 3729.713 kb on + strand, within Pf6N2E2_3224 at 3729.714 kb on - strand, within Pf6N2E2_3224 at 3729.993 kb on + strand, within Pf6N2E2_3224 at 3730.103 kb on - strand at 3730.289 kb on + strand at 3730.290 kb on - strand at 3730.290 kb on - strand at 3730.441 kb on - strand, within Pf6N2E2_3225 at 3730.441 kb on - strand, within Pf6N2E2_3225 at 3730.441 kb on - strand, within Pf6N2E2_3225 at 3730.441 kb on - strand, within Pf6N2E2_3225 at 3730.441 kb on - strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.443 kb on + strand, within Pf6N2E2_3225 at 3730.444 kb on - strand, within Pf6N2E2_3225 at 3730.444 kb on - strand, within Pf6N2E2_3225 at 3730.444 kb on - strand, within Pf6N2E2_3225
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 3,728,027 + +0.8 3,728,027 + +0.8 3,728,031 + +2.1 3,728,031 + +0.1 3,728,031 + -0.2 3,728,031 + +1.8 3,728,031 + +0.1 3,728,032 - -0.3 3,728,032 - -0.3 3,728,032 - -0.2 3,728,036 + +1.8 3,728,043 - +1.8 3,728,125 - -0.1 3,728,273 + Pf6N2E2_3222 0.25 -1.2 3,728,273 + Pf6N2E2_3222 0.25 -0.1 3,728,325 - Pf6N2E2_3222 0.32 +1.8 3,728,396 + Pf6N2E2_3222 0.41 +0.2 3,728,396 + Pf6N2E2_3222 0.41 -0.4 3,728,396 + Pf6N2E2_3222 0.41 -0.0 3,728,396 + Pf6N2E2_3222 0.41 +0.6 3,728,396 + Pf6N2E2_3222 0.41 -0.1 3,728,396 + Pf6N2E2_3222 0.41 -0.4 3,728,396 + Pf6N2E2_3222 0.41 -0.6 3,728,396 + Pf6N2E2_3222 0.41 -1.0 3,728,396 + Pf6N2E2_3222 0.41 -0.2 3,728,396 + Pf6N2E2_3222 0.41 +0.5 3,728,397 - Pf6N2E2_3222 0.41 -2.1 3,728,397 - Pf6N2E2_3222 0.41 -0.1 3,728,397 - Pf6N2E2_3222 0.41 +0.2 3,728,397 - Pf6N2E2_3222 0.41 +0.1 3,728,397 - Pf6N2E2_3222 0.41 +0.8 3,728,397 - Pf6N2E2_3222 0.41 -0.4 3,728,397 - Pf6N2E2_3222 0.41 -0.9 3,728,397 - Pf6N2E2_3222 0.41 +0.0 3,728,399 + Pf6N2E2_3222 0.41 +2.0 3,728,399 + Pf6N2E2_3222 0.41 +1.0 3,728,399 + Pf6N2E2_3222 0.41 +0.1 3,728,399 + Pf6N2E2_3222 0.41 -0.3 3,728,399 + Pf6N2E2_3222 0.41 -0.1 3,728,399 + Pf6N2E2_3222 0.41 -0.9 3,728,400 - Pf6N2E2_3222 0.42 +0.8 3,728,400 - Pf6N2E2_3222 0.42 +0.1 3,728,462 + Pf6N2E2_3222 0.50 -0.6 3,728,462 + Pf6N2E2_3222 0.50 -0.2 3,728,474 + Pf6N2E2_3222 0.51 -0.3 3,728,493 - Pf6N2E2_3222 0.54 -0.7 3,728,496 + Pf6N2E2_3222 0.54 +0.1 3,728,497 - Pf6N2E2_3222 0.54 +0.8 3,728,497 - Pf6N2E2_3222 0.54 +1.8 3,728,676 + Pf6N2E2_3222 0.78 -2.5 3,728,676 + Pf6N2E2_3222 0.78 +0.1 3,728,676 + Pf6N2E2_3222 0.78 -0.6 3,728,677 - Pf6N2E2_3222 0.78 +0.4 3,728,677 - Pf6N2E2_3222 0.78 -0.2 3,728,677 - Pf6N2E2_3222 0.78 +0.8 3,728,677 - Pf6N2E2_3222 0.78 -1.0 3,728,720 + Pf6N2E2_3222 0.84 +0.2 3,728,720 + Pf6N2E2_3222 0.84 +0.5 3,728,779 + +0.9 3,728,837 + -0.0 3,728,876 - +1.8 3,728,876 - +1.8 3,729,091 + Pf6N2E2_3223 0.34 -1.0 3,729,091 + Pf6N2E2_3223 0.34 +0.2 3,729,091 + Pf6N2E2_3223 0.34 -0.2 3,729,092 - Pf6N2E2_3223 0.34 +1.3 3,729,092 - Pf6N2E2_3223 0.34 +0.3 3,729,092 - Pf6N2E2_3223 0.34 +0.3 3,729,092 - Pf6N2E2_3223 0.34 +1.1 3,729,092 - Pf6N2E2_3223 0.34 +1.6 3,729,158 + Pf6N2E2_3223 0.45 -0.8 3,729,158 + Pf6N2E2_3223 0.45 +0.4 3,729,158 + Pf6N2E2_3223 0.45 -0.9 3,729,159 - Pf6N2E2_3223 0.45 -0.6 3,729,159 - Pf6N2E2_3223 0.45 -0.4 3,729,159 - Pf6N2E2_3223 0.45 -3.0 3,729,302 + Pf6N2E2_3223 0.68 -0.2 3,729,303 - Pf6N2E2_3223 0.68 +0.5 3,729,330 + Pf6N2E2_3223 0.72 +0.8 3,729,331 - Pf6N2E2_3223 0.72 +1.2 3,729,331 - Pf6N2E2_3223 0.72 +0.4 3,729,338 - Pf6N2E2_3223 0.73 +1.4 3,729,507 - -0.9 3,729,531 + +0.5 3,729,531 + -1.0 3,729,531 + +1.8 3,729,532 - -0.9 3,729,532 - -0.2 3,729,532 - +0.5 3,729,603 + -2.0 3,729,604 - +1.1 3,729,635 + +0.8 3,729,659 + +0.8 3,729,660 - -0.0 3,729,660 - -1.2 3,729,683 - Pf6N2E2_3224 0.11 +1.5 3,729,712 - Pf6N2E2_3224 0.18 -0.2 3,729,712 - Pf6N2E2_3224 0.18 +0.0 3,729,713 + Pf6N2E2_3224 0.18 -2.2 3,729,713 + Pf6N2E2_3224 0.18 -0.1 3,729,714 - Pf6N2E2_3224 0.19 +1.8 3,729,993 + Pf6N2E2_3224 0.86 +0.8 3,730,103 - -2.2 3,730,289 + +0.4 3,730,290 - -0.1 3,730,290 - -0.6 3,730,441 - Pf6N2E2_3225 0.20 -1.4 3,730,441 - Pf6N2E2_3225 0.20 +0.1 3,730,441 - Pf6N2E2_3225 0.20 -0.4 3,730,441 - Pf6N2E2_3225 0.20 +0.2 3,730,441 - Pf6N2E2_3225 0.20 -0.6 3,730,443 + Pf6N2E2_3225 0.20 -0.8 3,730,443 + Pf6N2E2_3225 0.20 -0.8 3,730,443 + Pf6N2E2_3225 0.20 +0.8 3,730,443 + Pf6N2E2_3225 0.20 -0.7 3,730,443 + Pf6N2E2_3225 0.20 -0.7 3,730,443 + Pf6N2E2_3225 0.20 -1.6 3,730,444 - Pf6N2E2_3225 0.20 -0.6 3,730,444 - Pf6N2E2_3225 0.20 +0.2 3,730,444 - Pf6N2E2_3225 0.20 -0.2
Or see this region's nucleotide sequence