Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2706 and Pf6N2E2_2707 overlap by 4 nucleotides Pf6N2E2_2707 and Pf6N2E2_2708 are separated by 8 nucleotides Pf6N2E2_2708 and Pf6N2E2_2709 are separated by 64 nucleotides
Pf6N2E2_2706 - Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54), at 3,199,240 to 3,200,160
_2706
Pf6N2E2_2707 - Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54), at 3,200,157 to 3,200,849
_2707
Pf6N2E2_2708 - Lactam utilization protein LamB, at 3,200,858 to 3,201,610
_2708
Pf6N2E2_2709 - Sialic acid transporter (permease) NanT, at 3,201,675 to 3,202,961
_2709
Position (kb)
3200
3201 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3199.316 kb on - strand at 3199.493 kb on + strand, within Pf6N2E2_2706 at 3199.494 kb on - strand, within Pf6N2E2_2706 at 3199.694 kb on + strand, within Pf6N2E2_2706 at 3199.695 kb on - strand, within Pf6N2E2_2706 at 3199.975 kb on - strand, within Pf6N2E2_2706 at 3200.483 kb on - strand, within Pf6N2E2_2707 at 3200.483 kb on - strand, within Pf6N2E2_2707 at 3200.576 kb on - strand, within Pf6N2E2_2707 at 3200.690 kb on - strand, within Pf6N2E2_2707 at 3200.690 kb on - strand, within Pf6N2E2_2707 at 3200.709 kb on - strand, within Pf6N2E2_2707 at 3200.710 kb on + strand, within Pf6N2E2_2707 at 3200.954 kb on - strand, within Pf6N2E2_2708 at 3200.954 kb on - strand, within Pf6N2E2_2708 at 3200.954 kb on - strand, within Pf6N2E2_2708 at 3201.090 kb on + strand, within Pf6N2E2_2708 at 3201.091 kb on - strand, within Pf6N2E2_2708 at 3201.204 kb on + strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.265 kb on - strand, within Pf6N2E2_2708 at 3201.288 kb on + strand, within Pf6N2E2_2708 at 3201.626 kb on - strand at 3201.626 kb on - strand at 3201.626 kb on - strand at 3201.626 kb on - strand at 3201.643 kb on - strand at 3201.643 kb on - strand at 3201.676 kb on + strand at 3201.676 kb on + strand at 3201.743 kb on - strand at 3201.743 kb on - strand at 3201.847 kb on + strand, within Pf6N2E2_2709
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 3,199,316 - -0.9 3,199,493 + Pf6N2E2_2706 0.27 +1.7 3,199,494 - Pf6N2E2_2706 0.28 -0.3 3,199,694 + Pf6N2E2_2706 0.49 -0.3 3,199,695 - Pf6N2E2_2706 0.49 -2.1 3,199,975 - Pf6N2E2_2706 0.80 -2.1 3,200,483 - Pf6N2E2_2707 0.47 +0.1 3,200,483 - Pf6N2E2_2707 0.47 -0.1 3,200,576 - Pf6N2E2_2707 0.60 -0.3 3,200,690 - Pf6N2E2_2707 0.77 -0.3 3,200,690 - Pf6N2E2_2707 0.77 -3.4 3,200,709 - Pf6N2E2_2707 0.80 -3.5 3,200,710 + Pf6N2E2_2707 0.80 +0.1 3,200,954 - Pf6N2E2_2708 0.13 -1.5 3,200,954 - Pf6N2E2_2708 0.13 +0.2 3,200,954 - Pf6N2E2_2708 0.13 -1.9 3,201,090 + Pf6N2E2_2708 0.31 +1.7 3,201,091 - Pf6N2E2_2708 0.31 +1.7 3,201,204 + Pf6N2E2_2708 0.46 +1.7 3,201,265 - Pf6N2E2_2708 0.54 -3.5 3,201,265 - Pf6N2E2_2708 0.54 -1.5 3,201,265 - Pf6N2E2_2708 0.54 -1.3 3,201,265 - Pf6N2E2_2708 0.54 -1.0 3,201,265 - Pf6N2E2_2708 0.54 +0.1 3,201,288 + Pf6N2E2_2708 0.57 -0.8 3,201,626 - -0.2 3,201,626 - +0.3 3,201,626 - +0.1 3,201,626 - +0.1 3,201,643 - -0.5 3,201,643 - -0.3 3,201,676 + -1.1 3,201,676 + +0.2 3,201,743 - +0.5 3,201,743 - -1.4 3,201,847 + Pf6N2E2_2709 0.13 -1.3
Or see this region's nucleotide sequence