Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2696 and Pf6N2E2_2697 are separated by 130 nucleotides Pf6N2E2_2697 and Pf6N2E2_2698 are separated by 119 nucleotides
Pf6N2E2_2696 - Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-), at 3,188,514 to 3,190,235
_2696
Pf6N2E2_2697 - Permease of the drug/metabolite transporter (DMT) superfamily, at 3,190,366 to 3,191,274
_2697
Pf6N2E2_2698 - Permease of the drug/metabolite transporter (DMT) superfamily, at 3,191,394 to 3,192,257
_2698
Position (kb)
3190
3191
3192 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3189.379 kb on + strand, within Pf6N2E2_2696 at 3189.379 kb on + strand, within Pf6N2E2_2696 at 3189.379 kb on + strand, within Pf6N2E2_2696 at 3189.380 kb on - strand, within Pf6N2E2_2696 at 3189.380 kb on - strand, within Pf6N2E2_2696 at 3189.463 kb on + strand, within Pf6N2E2_2696 at 3189.464 kb on - strand, within Pf6N2E2_2696 at 3189.607 kb on + strand, within Pf6N2E2_2696 at 3189.608 kb on - strand, within Pf6N2E2_2696 at 3189.758 kb on - strand, within Pf6N2E2_2696 at 3189.808 kb on + strand, within Pf6N2E2_2696 at 3189.808 kb on + strand, within Pf6N2E2_2696 at 3189.809 kb on - strand, within Pf6N2E2_2696 at 3189.809 kb on - strand, within Pf6N2E2_2696 at 3189.809 kb on - strand, within Pf6N2E2_2696 at 3189.809 kb on - strand, within Pf6N2E2_2696 at 3189.809 kb on - strand, within Pf6N2E2_2696 at 3189.809 kb on - strand, within Pf6N2E2_2696 at 3189.923 kb on - strand, within Pf6N2E2_2696 at 3189.971 kb on + strand, within Pf6N2E2_2696 at 3189.972 kb on - strand, within Pf6N2E2_2696 at 3189.972 kb on - strand, within Pf6N2E2_2696 at 3189.972 kb on - strand, within Pf6N2E2_2696 at 3189.973 kb on + strand, within Pf6N2E2_2696 at 3189.973 kb on + strand, within Pf6N2E2_2696 at 3189.973 kb on + strand, within Pf6N2E2_2696 at 3189.974 kb on - strand, within Pf6N2E2_2696 at 3190.072 kb on + strand at 3190.072 kb on + strand at 3190.072 kb on + strand at 3190.072 kb on + strand at 3190.072 kb on + strand at 3190.073 kb on - strand at 3190.073 kb on - strand at 3190.073 kb on - strand at 3190.073 kb on - strand at 3190.073 kb on - strand at 3190.235 kb on + strand at 3190.236 kb on - strand at 3190.241 kb on - strand at 3190.241 kb on - strand at 3190.264 kb on + strand at 3190.280 kb on + strand at 3190.392 kb on - strand at 3190.609 kb on + strand, within Pf6N2E2_2697 at 3190.609 kb on + strand, within Pf6N2E2_2697 at 3190.609 kb on + strand, within Pf6N2E2_2697 at 3190.609 kb on + strand, within Pf6N2E2_2697 at 3190.610 kb on - strand, within Pf6N2E2_2697 at 3190.642 kb on + strand, within Pf6N2E2_2697 at 3190.846 kb on + strand, within Pf6N2E2_2697 at 3190.846 kb on + strand, within Pf6N2E2_2697 at 3190.847 kb on - strand, within Pf6N2E2_2697 at 3190.966 kb on + strand, within Pf6N2E2_2697 at 3191.059 kb on + strand, within Pf6N2E2_2697 at 3191.060 kb on - strand, within Pf6N2E2_2697 at 3191.060 kb on - strand, within Pf6N2E2_2697 at 3191.060 kb on - strand, within Pf6N2E2_2697 at 3191.060 kb on - strand, within Pf6N2E2_2697 at 3191.068 kb on + strand, within Pf6N2E2_2697 at 3191.069 kb on - strand, within Pf6N2E2_2697 at 3191.069 kb on - strand, within Pf6N2E2_2697 at 3191.191 kb on + strand at 3191.191 kb on + strand at 3191.191 kb on + strand at 3191.192 kb on - strand at 3191.192 kb on - strand at 3191.368 kb on + strand at 3191.369 kb on - strand at 3191.370 kb on + strand at 3191.370 kb on + strand at 3191.371 kb on - strand at 3191.406 kb on - strand at 3191.406 kb on - strand at 3191.488 kb on + strand, within Pf6N2E2_2698 at 3191.489 kb on - strand, within Pf6N2E2_2698 at 3191.521 kb on + strand, within Pf6N2E2_2698 at 3191.522 kb on - strand, within Pf6N2E2_2698 at 3191.522 kb on - strand, within Pf6N2E2_2698 at 3191.522 kb on - strand, within Pf6N2E2_2698 at 3191.522 kb on - strand, within Pf6N2E2_2698 at 3191.522 kb on - strand, within Pf6N2E2_2698 at 3191.570 kb on - strand, within Pf6N2E2_2698 at 3191.642 kb on - strand, within Pf6N2E2_2698 at 3191.836 kb on + strand, within Pf6N2E2_2698 at 3191.836 kb on + strand, within Pf6N2E2_2698 at 3191.836 kb on + strand, within Pf6N2E2_2698 at 3191.836 kb on + strand, within Pf6N2E2_2698 at 3191.836 kb on + strand, within Pf6N2E2_2698 at 3191.837 kb on - strand, within Pf6N2E2_2698 at 3191.837 kb on - strand, within Pf6N2E2_2698 at 3192.261 kb on + strand at 3192.270 kb on + strand at 3192.270 kb on + strand at 3192.270 kb on + strand at 3192.271 kb on - strand at 3192.271 kb on - strand at 3192.271 kb on - strand at 3192.271 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 3,189,379 + Pf6N2E2_2696 0.50 -2.5 3,189,379 + Pf6N2E2_2696 0.50 -0.3 3,189,379 + Pf6N2E2_2696 0.50 +1.7 3,189,380 - Pf6N2E2_2696 0.50 -0.1 3,189,380 - Pf6N2E2_2696 0.50 -0.3 3,189,463 + Pf6N2E2_2696 0.55 -3.1 3,189,464 - Pf6N2E2_2696 0.55 +0.1 3,189,607 + Pf6N2E2_2696 0.63 -0.3 3,189,608 - Pf6N2E2_2696 0.64 -1.2 3,189,758 - Pf6N2E2_2696 0.72 +0.6 3,189,808 + Pf6N2E2_2696 0.75 -0.9 3,189,808 + Pf6N2E2_2696 0.75 -0.1 3,189,809 - Pf6N2E2_2696 0.75 +0.3 3,189,809 - Pf6N2E2_2696 0.75 -1.2 3,189,809 - Pf6N2E2_2696 0.75 +1.7 3,189,809 - Pf6N2E2_2696 0.75 +0.7 3,189,809 - Pf6N2E2_2696 0.75 +1.7 3,189,809 - Pf6N2E2_2696 0.75 -0.8 3,189,923 - Pf6N2E2_2696 0.82 -0.6 3,189,971 + Pf6N2E2_2696 0.85 +0.0 3,189,972 - Pf6N2E2_2696 0.85 +0.3 3,189,972 - Pf6N2E2_2696 0.85 -0.3 3,189,972 - Pf6N2E2_2696 0.85 -0.0 3,189,973 + Pf6N2E2_2696 0.85 -0.0 3,189,973 + Pf6N2E2_2696 0.85 +0.6 3,189,973 + Pf6N2E2_2696 0.85 -0.5 3,189,974 - Pf6N2E2_2696 0.85 -0.9 3,190,072 + +0.6 3,190,072 + -0.3 3,190,072 + -0.6 3,190,072 + -0.9 3,190,072 + -0.3 3,190,073 - +1.1 3,190,073 - -1.9 3,190,073 - -2.3 3,190,073 - +1.0 3,190,073 - -0.4 3,190,235 + -1.2 3,190,236 - +0.7 3,190,241 - +0.4 3,190,241 - +0.7 3,190,264 + -1.2 3,190,280 + -0.7 3,190,392 - +0.1 3,190,609 + Pf6N2E2_2697 0.27 +0.7 3,190,609 + Pf6N2E2_2697 0.27 -0.0 3,190,609 + Pf6N2E2_2697 0.27 -0.1 3,190,609 + Pf6N2E2_2697 0.27 -0.1 3,190,610 - Pf6N2E2_2697 0.27 -0.6 3,190,642 + Pf6N2E2_2697 0.30 +0.5 3,190,846 + Pf6N2E2_2697 0.53 +0.3 3,190,846 + Pf6N2E2_2697 0.53 -0.2 3,190,847 - Pf6N2E2_2697 0.53 -0.3 3,190,966 + Pf6N2E2_2697 0.66 -0.1 3,191,059 + Pf6N2E2_2697 0.76 -0.5 3,191,060 - Pf6N2E2_2697 0.76 +0.0 3,191,060 - Pf6N2E2_2697 0.76 -0.9 3,191,060 - Pf6N2E2_2697 0.76 -0.6 3,191,060 - Pf6N2E2_2697 0.76 -1.0 3,191,068 + Pf6N2E2_2697 0.77 +0.2 3,191,069 - Pf6N2E2_2697 0.77 -0.4 3,191,069 - Pf6N2E2_2697 0.77 +0.0 3,191,191 + -0.2 3,191,191 + +0.8 3,191,191 + +1.7 3,191,192 - +1.7 3,191,192 - +1.7 3,191,368 + -0.3 3,191,369 - -0.4 3,191,370 + +1.3 3,191,370 + -0.2 3,191,371 - +0.4 3,191,406 - -0.6 3,191,406 - -1.6 3,191,488 + Pf6N2E2_2698 0.11 -0.7 3,191,489 - Pf6N2E2_2698 0.11 +0.9 3,191,521 + Pf6N2E2_2698 0.15 +0.1 3,191,522 - Pf6N2E2_2698 0.15 -0.8 3,191,522 - Pf6N2E2_2698 0.15 -0.7 3,191,522 - Pf6N2E2_2698 0.15 +0.1 3,191,522 - Pf6N2E2_2698 0.15 -0.4 3,191,522 - Pf6N2E2_2698 0.15 +1.7 3,191,570 - Pf6N2E2_2698 0.20 -0.9 3,191,642 - Pf6N2E2_2698 0.29 +0.1 3,191,836 + Pf6N2E2_2698 0.51 +0.6 3,191,836 + Pf6N2E2_2698 0.51 -1.0 3,191,836 + Pf6N2E2_2698 0.51 -1.3 3,191,836 + Pf6N2E2_2698 0.51 -0.1 3,191,836 + Pf6N2E2_2698 0.51 +1.7 3,191,837 - Pf6N2E2_2698 0.51 +1.7 3,191,837 - Pf6N2E2_2698 0.51 -0.2 3,192,261 + -1.9 3,192,270 + -2.4 3,192,270 + -1.0 3,192,270 + +0.7 3,192,271 - -0.5 3,192,271 - -1.9 3,192,271 - -1.2 3,192,271 - -0.2
Or see this region's nucleotide sequence