Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2675 and Pf6N2E2_2676 are separated by 151 nucleotides Pf6N2E2_2676 and Pf6N2E2_2677 overlap by 72 nucleotides Pf6N2E2_2677 and Pf6N2E2_2678 are separated by 109 nucleotides
Pf6N2E2_2675 - ATP-dependent RNA helicase SrmB, at 3,163,442 to 3,164,788
_2675
Pf6N2E2_2676 - hypothetical protein, at 3,164,940 to 3,165,053
_2676
Pf6N2E2_2677 - Small-conductance mechanosensitive channel, at 3,164,982 to 3,166,280
_2677
Pf6N2E2_2678 - MadN protein, at 3,166,390 to 3,167,256
_2678
Position (kb)
3164
3165
3166
3167 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3164.827 kb on - strand at 3164.866 kb on - strand at 3164.932 kb on + strand at 3164.932 kb on + strand at 3164.932 kb on + strand at 3164.942 kb on - strand at 3164.964 kb on - strand, within Pf6N2E2_2676 at 3164.964 kb on - strand, within Pf6N2E2_2676 at 3165.366 kb on + strand, within Pf6N2E2_2677 at 3165.366 kb on + strand, within Pf6N2E2_2677 at 3165.408 kb on + strand, within Pf6N2E2_2677 at 3165.408 kb on + strand, within Pf6N2E2_2677 at 3165.408 kb on + strand, within Pf6N2E2_2677 at 3165.408 kb on + strand, within Pf6N2E2_2677 at 3165.408 kb on + strand, within Pf6N2E2_2677 at 3165.408 kb on + strand, within Pf6N2E2_2677 at 3165.435 kb on + strand, within Pf6N2E2_2677 at 3165.435 kb on + strand, within Pf6N2E2_2677 at 3165.435 kb on + strand, within Pf6N2E2_2677 at 3165.435 kb on + strand, within Pf6N2E2_2677 at 3165.435 kb on + strand, within Pf6N2E2_2677 at 3165.435 kb on + strand, within Pf6N2E2_2677 at 3165.435 kb on + strand, within Pf6N2E2_2677 at 3165.435 kb on + strand, within Pf6N2E2_2677 at 3165.435 kb on + strand, within Pf6N2E2_2677 at 3165.435 kb on + strand, within Pf6N2E2_2677 at 3165.435 kb on + strand, within Pf6N2E2_2677 at 3165.435 kb on + strand, within Pf6N2E2_2677 at 3165.436 kb on - strand, within Pf6N2E2_2677 at 3165.436 kb on - strand, within Pf6N2E2_2677 at 3165.436 kb on - strand, within Pf6N2E2_2677 at 3165.436 kb on - strand, within Pf6N2E2_2677 at 3165.436 kb on - strand, within Pf6N2E2_2677 at 3165.436 kb on - strand, within Pf6N2E2_2677 at 3165.436 kb on - strand, within Pf6N2E2_2677 at 3165.439 kb on + strand, within Pf6N2E2_2677 at 3165.439 kb on + strand, within Pf6N2E2_2677 at 3165.440 kb on - strand, within Pf6N2E2_2677 at 3165.537 kb on + strand, within Pf6N2E2_2677 at 3165.538 kb on - strand, within Pf6N2E2_2677 at 3165.538 kb on - strand, within Pf6N2E2_2677 at 3165.870 kb on + strand, within Pf6N2E2_2677 at 3165.871 kb on - strand, within Pf6N2E2_2677 at 3165.982 kb on - strand, within Pf6N2E2_2677 at 3165.994 kb on - strand, within Pf6N2E2_2677 at 3165.994 kb on - strand, within Pf6N2E2_2677 at 3166.084 kb on - strand, within Pf6N2E2_2677 at 3166.084 kb on - strand, within Pf6N2E2_2677 at 3166.116 kb on + strand, within Pf6N2E2_2677 at 3166.116 kb on + strand, within Pf6N2E2_2677 at 3166.137 kb on - strand, within Pf6N2E2_2677 at 3166.137 kb on - strand, within Pf6N2E2_2677 at 3166.146 kb on + strand, within Pf6N2E2_2677 at 3166.340 kb on + strand at 3166.341 kb on - strand at 3166.341 kb on - strand at 3166.341 kb on - strand at 3166.341 kb on - strand at 3166.341 kb on - strand at 3166.341 kb on - strand at 3166.724 kb on + strand, within Pf6N2E2_2678 at 3166.724 kb on + strand, within Pf6N2E2_2678 at 3166.724 kb on + strand, within Pf6N2E2_2678 at 3166.787 kb on + strand, within Pf6N2E2_2678 at 3166.787 kb on + strand, within Pf6N2E2_2678 at 3166.788 kb on - strand, within Pf6N2E2_2678 at 3166.788 kb on - strand, within Pf6N2E2_2678 at 3166.788 kb on - strand, within Pf6N2E2_2678 at 3166.788 kb on - strand, within Pf6N2E2_2678 at 3166.788 kb on - strand, within Pf6N2E2_2678 at 3166.788 kb on - strand, within Pf6N2E2_2678 at 3166.808 kb on + strand, within Pf6N2E2_2678 at 3166.808 kb on + strand, within Pf6N2E2_2678 at 3166.808 kb on + strand, within Pf6N2E2_2678 at 3166.808 kb on + strand, within Pf6N2E2_2678 at 3166.809 kb on - strand, within Pf6N2E2_2678 at 3166.809 kb on - strand, within Pf6N2E2_2678 at 3166.809 kb on - strand, within Pf6N2E2_2678 at 3166.809 kb on - strand, within Pf6N2E2_2678 at 3167.030 kb on + strand, within Pf6N2E2_2678 at 3167.030 kb on + strand, within Pf6N2E2_2678 at 3167.031 kb on - strand, within Pf6N2E2_2678 at 3167.031 kb on - strand, within Pf6N2E2_2678 at 3167.031 kb on - strand, within Pf6N2E2_2678 at 3167.043 kb on - strand, within Pf6N2E2_2678 at 3167.043 kb on - strand, within Pf6N2E2_2678 at 3167.043 kb on - strand, within Pf6N2E2_2678 at 3167.043 kb on - strand, within Pf6N2E2_2678 at 3167.043 kb on - strand, within Pf6N2E2_2678 at 3167.043 kb on - strand, within Pf6N2E2_2678 at 3167.043 kb on - strand, within Pf6N2E2_2678 at 3167.171 kb on + strand at 3167.171 kb on + strand at 3167.171 kb on + strand at 3167.171 kb on + strand at 3167.195 kb on + strand at 3167.245 kb on + strand at 3167.246 kb on - strand at 3167.246 kb on - strand at 3167.246 kb on - strand at 3167.249 kb on + strand at 3167.249 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 3,164,827 - -0.4 3,164,866 - -0.6 3,164,932 + -0.3 3,164,932 + +0.1 3,164,932 + +1.4 3,164,942 - -1.3 3,164,964 - Pf6N2E2_2676 0.21 -2.3 3,164,964 - Pf6N2E2_2676 0.21 +0.9 3,165,366 + Pf6N2E2_2677 0.30 -1.5 3,165,366 + Pf6N2E2_2677 0.30 +1.7 3,165,408 + Pf6N2E2_2677 0.33 -0.5 3,165,408 + Pf6N2E2_2677 0.33 -0.9 3,165,408 + Pf6N2E2_2677 0.33 -1.3 3,165,408 + Pf6N2E2_2677 0.33 +0.7 3,165,408 + Pf6N2E2_2677 0.33 -1.5 3,165,408 + Pf6N2E2_2677 0.33 +0.1 3,165,435 + Pf6N2E2_2677 0.35 +0.1 3,165,435 + Pf6N2E2_2677 0.35 -2.4 3,165,435 + Pf6N2E2_2677 0.35 +1.7 3,165,435 + Pf6N2E2_2677 0.35 +0.3 3,165,435 + Pf6N2E2_2677 0.35 -0.2 3,165,435 + Pf6N2E2_2677 0.35 -3.0 3,165,435 + Pf6N2E2_2677 0.35 -2.0 3,165,435 + Pf6N2E2_2677 0.35 -1.3 3,165,435 + Pf6N2E2_2677 0.35 -1.3 3,165,435 + Pf6N2E2_2677 0.35 +1.7 3,165,435 + Pf6N2E2_2677 0.35 +0.3 3,165,435 + Pf6N2E2_2677 0.35 -1.2 3,165,436 - Pf6N2E2_2677 0.35 +1.7 3,165,436 - Pf6N2E2_2677 0.35 -1.6 3,165,436 - Pf6N2E2_2677 0.35 -0.9 3,165,436 - Pf6N2E2_2677 0.35 -2.7 3,165,436 - Pf6N2E2_2677 0.35 -0.2 3,165,436 - Pf6N2E2_2677 0.35 -1.6 3,165,436 - Pf6N2E2_2677 0.35 -1.5 3,165,439 + Pf6N2E2_2677 0.35 -0.1 3,165,439 + Pf6N2E2_2677 0.35 -1.1 3,165,440 - Pf6N2E2_2677 0.35 +0.5 3,165,537 + Pf6N2E2_2677 0.43 -0.9 3,165,538 - Pf6N2E2_2677 0.43 -0.8 3,165,538 - Pf6N2E2_2677 0.43 +0.8 3,165,870 + Pf6N2E2_2677 0.68 -0.4 3,165,871 - Pf6N2E2_2677 0.68 +0.1 3,165,982 - Pf6N2E2_2677 0.77 -0.9 3,165,994 - Pf6N2E2_2677 0.78 -1.6 3,165,994 - Pf6N2E2_2677 0.78 +0.1 3,166,084 - Pf6N2E2_2677 0.85 -0.1 3,166,084 - Pf6N2E2_2677 0.85 -2.0 3,166,116 + Pf6N2E2_2677 0.87 +1.7 3,166,116 + Pf6N2E2_2677 0.87 +0.4 3,166,137 - Pf6N2E2_2677 0.89 -0.2 3,166,137 - Pf6N2E2_2677 0.89 -1.5 3,166,146 + Pf6N2E2_2677 0.90 +0.1 3,166,340 + +0.1 3,166,341 - -0.4 3,166,341 - -1.5 3,166,341 - -3.2 3,166,341 - +1.7 3,166,341 - +1.0 3,166,341 - -1.3 3,166,724 + Pf6N2E2_2678 0.39 -1.1 3,166,724 + Pf6N2E2_2678 0.39 -1.9 3,166,724 + Pf6N2E2_2678 0.39 -0.3 3,166,787 + Pf6N2E2_2678 0.46 +1.4 3,166,787 + Pf6N2E2_2678 0.46 +1.7 3,166,788 - Pf6N2E2_2678 0.46 -0.4 3,166,788 - Pf6N2E2_2678 0.46 -1.4 3,166,788 - Pf6N2E2_2678 0.46 +0.1 3,166,788 - Pf6N2E2_2678 0.46 -1.8 3,166,788 - Pf6N2E2_2678 0.46 -2.1 3,166,788 - Pf6N2E2_2678 0.46 +1.3 3,166,808 + Pf6N2E2_2678 0.48 +1.7 3,166,808 + Pf6N2E2_2678 0.48 -0.3 3,166,808 + Pf6N2E2_2678 0.48 +0.5 3,166,808 + Pf6N2E2_2678 0.48 +1.7 3,166,809 - Pf6N2E2_2678 0.48 +1.7 3,166,809 - Pf6N2E2_2678 0.48 +0.3 3,166,809 - Pf6N2E2_2678 0.48 -0.9 3,166,809 - Pf6N2E2_2678 0.48 +0.6 3,167,030 + Pf6N2E2_2678 0.74 +0.7 3,167,030 + Pf6N2E2_2678 0.74 +1.1 3,167,031 - Pf6N2E2_2678 0.74 +0.8 3,167,031 - Pf6N2E2_2678 0.74 -0.9 3,167,031 - Pf6N2E2_2678 0.74 +0.0 3,167,043 - Pf6N2E2_2678 0.75 -0.2 3,167,043 - Pf6N2E2_2678 0.75 -2.0 3,167,043 - Pf6N2E2_2678 0.75 -1.4 3,167,043 - Pf6N2E2_2678 0.75 -0.0 3,167,043 - Pf6N2E2_2678 0.75 -0.2 3,167,043 - Pf6N2E2_2678 0.75 -0.3 3,167,043 - Pf6N2E2_2678 0.75 -0.3 3,167,171 + -0.2 3,167,171 + +1.0 3,167,171 + -0.1 3,167,171 + -0.2 3,167,195 + -0.1 3,167,245 + +0.1 3,167,246 - -0.3 3,167,246 - -2.3 3,167,246 - +0.1 3,167,249 + -1.8 3,167,249 + +0.7
Or see this region's nucleotide sequence