Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2409 and Pf6N2E2_2410 are separated by 33 nucleotides Pf6N2E2_2410 and Pf6N2E2_2411 are separated by 134 nucleotides
Pf6N2E2_2409 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 2,860,579 to 2,862,195
_2409
Pf6N2E2_2410 - Citronellyl-CoA dehydrogenase, at 2,862,229 to 2,863,386
_2410
Pf6N2E2_2411 - Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4), at 2,863,521 to 2,865,491
_2411
Position (kb)
2862
2863
2864 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2861.314 kb on + strand, within Pf6N2E2_2409 at 2861.314 kb on + strand, within Pf6N2E2_2409 at 2861.315 kb on - strand, within Pf6N2E2_2409 at 2861.429 kb on - strand, within Pf6N2E2_2409 at 2861.444 kb on + strand, within Pf6N2E2_2409 at 2861.444 kb on + strand, within Pf6N2E2_2409 at 2861.479 kb on + strand, within Pf6N2E2_2409 at 2861.480 kb on - strand, within Pf6N2E2_2409 at 2861.480 kb on - strand, within Pf6N2E2_2409 at 2861.480 kb on - strand, within Pf6N2E2_2409 at 2861.842 kb on + strand, within Pf6N2E2_2409 at 2861.842 kb on + strand, within Pf6N2E2_2409 at 2861.842 kb on + strand, within Pf6N2E2_2409 at 2862.056 kb on - strand at 2862.121 kb on + strand at 2862.301 kb on + strand at 2862.302 kb on - strand at 2862.302 kb on - strand at 2862.421 kb on + strand, within Pf6N2E2_2410 at 2862.422 kb on - strand, within Pf6N2E2_2410 at 2862.422 kb on - strand, within Pf6N2E2_2410 at 2862.422 kb on - strand, within Pf6N2E2_2410 at 2862.422 kb on - strand, within Pf6N2E2_2410 at 2862.427 kb on + strand, within Pf6N2E2_2410 at 2862.427 kb on + strand, within Pf6N2E2_2410 at 2862.428 kb on - strand, within Pf6N2E2_2410 at 2862.670 kb on + strand, within Pf6N2E2_2410 at 2862.670 kb on + strand, within Pf6N2E2_2410 at 2862.670 kb on + strand, within Pf6N2E2_2410 at 2862.671 kb on - strand, within Pf6N2E2_2410 at 2862.671 kb on - strand, within Pf6N2E2_2410 at 2862.671 kb on - strand, within Pf6N2E2_2410 at 2862.671 kb on - strand, within Pf6N2E2_2410 at 2862.671 kb on - strand, within Pf6N2E2_2410 at 2862.741 kb on + strand, within Pf6N2E2_2410 at 2862.742 kb on - strand, within Pf6N2E2_2410 at 2862.742 kb on - strand, within Pf6N2E2_2410 at 2862.742 kb on - strand, within Pf6N2E2_2410 at 2863.018 kb on + strand, within Pf6N2E2_2410 at 2863.019 kb on - strand, within Pf6N2E2_2410 at 2863.144 kb on + strand, within Pf6N2E2_2410 at 2863.145 kb on - strand, within Pf6N2E2_2410 at 2863.145 kb on - strand, within Pf6N2E2_2410 at 2863.145 kb on - strand, within Pf6N2E2_2410 at 2863.145 kb on - strand, within Pf6N2E2_2410 at 2863.237 kb on + strand, within Pf6N2E2_2410 at 2863.490 kb on - strand at 2863.704 kb on + strand at 2863.704 kb on + strand at 2863.705 kb on - strand at 2863.705 kb on - strand at 2864.328 kb on + strand, within Pf6N2E2_2411 at 2864.328 kb on + strand, within Pf6N2E2_2411 at 2864.328 kb on + strand, within Pf6N2E2_2411 at 2864.328 kb on + strand, within Pf6N2E2_2411 at 2864.328 kb on + strand, within Pf6N2E2_2411 at 2864.379 kb on + strand, within Pf6N2E2_2411 at 2864.379 kb on + strand, within Pf6N2E2_2411
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 2,861,314 + Pf6N2E2_2409 0.45 +0.9 2,861,314 + Pf6N2E2_2409 0.45 -2.5 2,861,315 - Pf6N2E2_2409 0.46 +1.9 2,861,429 - Pf6N2E2_2409 0.53 -0.9 2,861,444 + Pf6N2E2_2409 0.53 +0.7 2,861,444 + Pf6N2E2_2409 0.53 -0.2 2,861,479 + Pf6N2E2_2409 0.56 -1.3 2,861,480 - Pf6N2E2_2409 0.56 +1.1 2,861,480 - Pf6N2E2_2409 0.56 -0.2 2,861,480 - Pf6N2E2_2409 0.56 -3.0 2,861,842 + Pf6N2E2_2409 0.78 +0.1 2,861,842 + Pf6N2E2_2409 0.78 -0.5 2,861,842 + Pf6N2E2_2409 0.78 -0.9 2,862,056 - -0.6 2,862,121 + +0.7 2,862,301 + +0.1 2,862,302 - -2.2 2,862,302 - +0.2 2,862,421 + Pf6N2E2_2410 0.17 -0.6 2,862,422 - Pf6N2E2_2410 0.17 +1.7 2,862,422 - Pf6N2E2_2410 0.17 +0.1 2,862,422 - Pf6N2E2_2410 0.17 -1.9 2,862,422 - Pf6N2E2_2410 0.17 +0.5 2,862,427 + Pf6N2E2_2410 0.17 -0.7 2,862,427 + Pf6N2E2_2410 0.17 -0.1 2,862,428 - Pf6N2E2_2410 0.17 -0.3 2,862,670 + Pf6N2E2_2410 0.38 -0.6 2,862,670 + Pf6N2E2_2410 0.38 +1.7 2,862,670 + Pf6N2E2_2410 0.38 -1.4 2,862,671 - Pf6N2E2_2410 0.38 -0.7 2,862,671 - Pf6N2E2_2410 0.38 -0.4 2,862,671 - Pf6N2E2_2410 0.38 +1.7 2,862,671 - Pf6N2E2_2410 0.38 -0.1 2,862,671 - Pf6N2E2_2410 0.38 -0.3 2,862,741 + Pf6N2E2_2410 0.44 +0.1 2,862,742 - Pf6N2E2_2410 0.44 +0.6 2,862,742 - Pf6N2E2_2410 0.44 -0.1 2,862,742 - Pf6N2E2_2410 0.44 -0.2 2,863,018 + Pf6N2E2_2410 0.68 -0.8 2,863,019 - Pf6N2E2_2410 0.68 -1.5 2,863,144 + Pf6N2E2_2410 0.79 -2.4 2,863,145 - Pf6N2E2_2410 0.79 -0.2 2,863,145 - Pf6N2E2_2410 0.79 -2.4 2,863,145 - Pf6N2E2_2410 0.79 -1.9 2,863,145 - Pf6N2E2_2410 0.79 +0.4 2,863,237 + Pf6N2E2_2410 0.87 -1.4 2,863,490 - +0.8 2,863,704 + -0.6 2,863,704 + -0.5 2,863,705 - +0.1 2,863,705 - +0.2 2,864,328 + Pf6N2E2_2411 0.41 +1.2 2,864,328 + Pf6N2E2_2411 0.41 -0.2 2,864,328 + Pf6N2E2_2411 0.41 +1.1 2,864,328 + Pf6N2E2_2411 0.41 +0.2 2,864,328 + Pf6N2E2_2411 0.41 +0.1 2,864,379 + Pf6N2E2_2411 0.44 -0.6 2,864,379 + Pf6N2E2_2411 0.44 +0.2
Or see this region's nucleotide sequence