Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1986 and Pf6N2E2_1987 are separated by 338 nucleotides Pf6N2E2_1987 and Pf6N2E2_1988 overlap by 4 nucleotides Pf6N2E2_1988 and Pf6N2E2_1989 are separated by 118 nucleotides Pf6N2E2_1989 and Pf6N2E2_1990 are separated by 404 nucleotides
Pf6N2E2_1986 - putative membrane protein, at 2,353,114 to 2,353,863
_1986
Pf6N2E2_1987 - hypothetical protein, at 2,354,202 to 2,354,321
_1987
Pf6N2E2_1988 - hypothetical protein, at 2,354,318 to 2,354,551
_1988
Pf6N2E2_1989 - hypothetical protein, at 2,354,670 to 2,354,927
_1989
Pf6N2E2_1990 - Possible exported protein, at 2,355,332 to 2,356,015
_1990
Position (kb)
2354
2355 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2353.693 kb on + strand, within Pf6N2E2_1986 at 2353.797 kb on - strand at 2353.856 kb on - strand at 2353.879 kb on + strand at 2353.959 kb on + strand at 2354.041 kb on + strand at 2354.084 kb on + strand at 2354.085 kb on - strand at 2354.092 kb on + strand at 2354.187 kb on + strand at 2354.188 kb on - strand at 2354.283 kb on + strand, within Pf6N2E2_1987 at 2354.284 kb on - strand, within Pf6N2E2_1987 at 2354.519 kb on + strand, within Pf6N2E2_1988 at 2354.724 kb on + strand, within Pf6N2E2_1989 at 2354.725 kb on - strand, within Pf6N2E2_1989 at 2354.725 kb on - strand, within Pf6N2E2_1989 at 2354.800 kb on - strand, within Pf6N2E2_1989 at 2355.140 kb on - strand at 2355.252 kb on + strand at 2355.294 kb on - strand at 2355.294 kb on - strand at 2355.294 kb on - strand at 2355.460 kb on - strand, within Pf6N2E2_1990 at 2355.503 kb on - strand, within Pf6N2E2_1990 at 2355.548 kb on + strand, within Pf6N2E2_1990 at 2355.549 kb on - strand, within Pf6N2E2_1990 at 2355.596 kb on + strand, within Pf6N2E2_1990 at 2355.596 kb on + strand, within Pf6N2E2_1990 at 2355.627 kb on - strand, within Pf6N2E2_1990 at 2355.665 kb on + strand, within Pf6N2E2_1990 at 2355.665 kb on + strand, within Pf6N2E2_1990 at 2355.684 kb on + strand, within Pf6N2E2_1990 at 2355.684 kb on + strand, within Pf6N2E2_1990 at 2355.685 kb on - strand, within Pf6N2E2_1990 at 2355.717 kb on - strand, within Pf6N2E2_1990 at 2355.717 kb on - strand, within Pf6N2E2_1990 at 2355.717 kb on - strand, within Pf6N2E2_1990 at 2355.769 kb on - strand, within Pf6N2E2_1990 at 2355.793 kb on + strand, within Pf6N2E2_1990 at 2355.873 kb on + strand, within Pf6N2E2_1990
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 2,353,693 + Pf6N2E2_1986 0.77 -0.2 2,353,797 - -1.5 2,353,856 - +0.6 2,353,879 + +0.8 2,353,959 + -0.3 2,354,041 + -2.4 2,354,084 + -0.9 2,354,085 - -2.4 2,354,092 + +0.6 2,354,187 + -1.4 2,354,188 - -0.1 2,354,283 + Pf6N2E2_1987 0.68 -1.2 2,354,284 - Pf6N2E2_1987 0.68 +1.6 2,354,519 + Pf6N2E2_1988 0.86 -1.8 2,354,724 + Pf6N2E2_1989 0.21 -1.2 2,354,725 - Pf6N2E2_1989 0.21 -1.2 2,354,725 - Pf6N2E2_1989 0.21 +0.1 2,354,800 - Pf6N2E2_1989 0.50 -1.5 2,355,140 - +1.6 2,355,252 + +3.2 2,355,294 - +0.6 2,355,294 - +0.2 2,355,294 - +0.0 2,355,460 - Pf6N2E2_1990 0.19 -1.7 2,355,503 - Pf6N2E2_1990 0.25 +1.6 2,355,548 + Pf6N2E2_1990 0.32 -0.3 2,355,549 - Pf6N2E2_1990 0.32 +0.6 2,355,596 + Pf6N2E2_1990 0.39 +1.6 2,355,596 + Pf6N2E2_1990 0.39 +1.2 2,355,627 - Pf6N2E2_1990 0.43 +0.1 2,355,665 + Pf6N2E2_1990 0.49 +0.1 2,355,665 + Pf6N2E2_1990 0.49 -0.2 2,355,684 + Pf6N2E2_1990 0.51 -1.2 2,355,684 + Pf6N2E2_1990 0.51 +0.3 2,355,685 - Pf6N2E2_1990 0.52 +0.5 2,355,717 - Pf6N2E2_1990 0.56 +0.9 2,355,717 - Pf6N2E2_1990 0.56 -0.3 2,355,717 - Pf6N2E2_1990 0.56 +0.1 2,355,769 - Pf6N2E2_1990 0.64 +1.6 2,355,793 + Pf6N2E2_1990 0.67 -0.3 2,355,873 + Pf6N2E2_1990 0.79 +1.6
Or see this region's nucleotide sequence