Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1749 and Pf6N2E2_1750 are separated by 162 nucleotides Pf6N2E2_1750 and Pf6N2E2_1751 are separated by 64 nucleotides
Pf6N2E2_1749 - Cyn operon transcriptional activator, at 2,062,810 to 2,063,703
_1749
Pf6N2E2_1750 - Sigma-54 dependent transcriptional regulator, at 2,063,866 to 2,065,788
_1750
Pf6N2E2_1751 - Aldehyde dehydrogenase A (EC 1.2.1.22) / Glycolaldehyde dehydrogenase (EC 1.2.1.21), at 2,065,853 to 2,067,286
_1751
Position (kb)
2063
2064
2065
2066 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2063.032 kb on - strand, within Pf6N2E2_1749 at 2063.327 kb on - strand, within Pf6N2E2_1749 at 2063.385 kb on - strand, within Pf6N2E2_1749 at 2063.385 kb on - strand, within Pf6N2E2_1749 at 2063.702 kb on - strand at 2063.702 kb on - strand at 2063.775 kb on + strand at 2063.776 kb on - strand at 2063.781 kb on - strand at 2063.811 kb on + strand at 2063.812 kb on - strand at 2063.852 kb on - strand at 2064.077 kb on - strand, within Pf6N2E2_1750 at 2064.077 kb on - strand, within Pf6N2E2_1750 at 2064.205 kb on + strand, within Pf6N2E2_1750 at 2064.205 kb on + strand, within Pf6N2E2_1750 at 2064.206 kb on - strand, within Pf6N2E2_1750 at 2064.206 kb on - strand, within Pf6N2E2_1750 at 2064.442 kb on + strand, within Pf6N2E2_1750 at 2064.443 kb on - strand, within Pf6N2E2_1750 at 2064.443 kb on - strand, within Pf6N2E2_1750 at 2064.708 kb on - strand, within Pf6N2E2_1750 at 2065.092 kb on + strand, within Pf6N2E2_1750 at 2065.308 kb on + strand, within Pf6N2E2_1750 at 2065.308 kb on + strand, within Pf6N2E2_1750 at 2065.309 kb on - strand, within Pf6N2E2_1750 at 2065.309 kb on - strand, within Pf6N2E2_1750 at 2065.610 kb on - strand at 2065.866 kb on + strand at 2065.866 kb on + strand at 2065.866 kb on + strand at 2065.866 kb on + strand at 2065.867 kb on - strand at 2065.893 kb on + strand at 2065.985 kb on - strand at 2065.986 kb on + strand at 2065.986 kb on + strand at 2065.986 kb on + strand at 2065.986 kb on + strand at 2065.986 kb on + strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2066.047 kb on - strand, within Pf6N2E2_1751 at 2066.243 kb on - strand, within Pf6N2E2_1751 at 2066.782 kb on + strand, within Pf6N2E2_1751
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 2,063,032 - Pf6N2E2_1749 0.25 +0.5 2,063,327 - Pf6N2E2_1749 0.58 -0.6 2,063,385 - Pf6N2E2_1749 0.64 +0.5 2,063,385 - Pf6N2E2_1749 0.64 -0.3 2,063,702 - +1.5 2,063,702 - -0.4 2,063,775 + -0.5 2,063,776 - -1.0 2,063,781 - +1.5 2,063,811 + -2.0 2,063,812 - +1.5 2,063,852 - +1.5 2,064,077 - Pf6N2E2_1750 0.11 +0.0 2,064,077 - Pf6N2E2_1750 0.11 +1.1 2,064,205 + Pf6N2E2_1750 0.18 +0.2 2,064,205 + Pf6N2E2_1750 0.18 +1.5 2,064,206 - Pf6N2E2_1750 0.18 +1.5 2,064,206 - Pf6N2E2_1750 0.18 +1.5 2,064,442 + Pf6N2E2_1750 0.30 +1.5 2,064,443 - Pf6N2E2_1750 0.30 -0.9 2,064,443 - Pf6N2E2_1750 0.30 +0.7 2,064,708 - Pf6N2E2_1750 0.44 +0.1 2,065,092 + Pf6N2E2_1750 0.64 +2.3 2,065,308 + Pf6N2E2_1750 0.75 -0.2 2,065,308 + Pf6N2E2_1750 0.75 +1.1 2,065,309 - Pf6N2E2_1750 0.75 +1.5 2,065,309 - Pf6N2E2_1750 0.75 +0.7 2,065,610 - -0.5 2,065,866 + -0.5 2,065,866 + -0.7 2,065,866 + +0.3 2,065,866 + +1.5 2,065,867 - -1.3 2,065,893 + +0.3 2,065,985 - -0.0 2,065,986 + -0.1 2,065,986 + +0.7 2,065,986 + +1.5 2,065,986 + -0.9 2,065,986 + -0.3 2,065,987 - +0.1 2,065,987 - +1.5 2,065,987 - -0.9 2,065,987 - -0.1 2,065,987 - -1.0 2,065,987 - -0.9 2,065,987 - +1.5 2,065,987 - -0.7 2,066,047 - Pf6N2E2_1751 0.14 -1.1 2,066,243 - Pf6N2E2_1751 0.27 -0.5 2,066,782 + Pf6N2E2_1751 0.65 +0.0
Or see this region's nucleotide sequence