Experiment: nitrogen source nitrite 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_165 and Pf6N2E2_166 overlap by 4 nucleotides Pf6N2E2_166 and Pf6N2E2_167 are separated by 30 nucleotides Pf6N2E2_167 and Pf6N2E2_168 are separated by 48 nucleotides Pf6N2E2_168 and Pf6N2E2_169 are separated by 128 nucleotides Pf6N2E2_169 and Pf6N2E2_170 are separated by 162 nucleotides
Pf6N2E2_165 - Ribokinase (EC 2.7.1.15), at 218,870 to 219,784
_165
Pf6N2E2_166 - Ribose ABC transport system, high affinity permease RbsD (TC 3.A.1.2.1), at 219,781 to 220,185
_166
Pf6N2E2_167 - Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1), at 220,216 to 221,244
_167
Pf6N2E2_168 - hypothetical protein, at 221,293 to 221,490
_168
Pf6N2E2_169 - FIG00955358: hypothetical protein, at 221,619 to 221,924
_169
Pf6N2E2_170 - lipoprotein, putative, at 222,087 to 222,401
_170
Position (kb)
220
221
222 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 219.377 kb on - strand, within Pf6N2E2_165 at 219.395 kb on + strand, within Pf6N2E2_165 at 219.421 kb on + strand, within Pf6N2E2_165 at 219.421 kb on + strand, within Pf6N2E2_165 at 219.421 kb on + strand, within Pf6N2E2_165 at 219.421 kb on + strand, within Pf6N2E2_165 at 219.765 kb on + strand at 219.766 kb on - strand at 219.766 kb on - strand at 219.766 kb on - strand at 220.184 kb on - strand at 220.184 kb on - strand at 220.184 kb on - strand at 220.226 kb on - strand at 220.393 kb on - strand, within Pf6N2E2_167 at 220.393 kb on - strand, within Pf6N2E2_167 at 220.393 kb on - strand, within Pf6N2E2_167 at 220.393 kb on - strand, within Pf6N2E2_167 at 220.495 kb on + strand, within Pf6N2E2_167 at 220.496 kb on - strand, within Pf6N2E2_167 at 220.580 kb on + strand, within Pf6N2E2_167 at 220.581 kb on - strand, within Pf6N2E2_167 at 220.581 kb on - strand, within Pf6N2E2_167 at 220.624 kb on + strand, within Pf6N2E2_167 at 220.785 kb on + strand, within Pf6N2E2_167 at 220.785 kb on + strand, within Pf6N2E2_167 at 220.867 kb on + strand, within Pf6N2E2_167 at 220.978 kb on + strand, within Pf6N2E2_167 at 220.978 kb on + strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 221.002 kb on + strand, within Pf6N2E2_167 at 221.002 kb on + strand, within Pf6N2E2_167 at 221.144 kb on - strand at 221.144 kb on - strand at 221.255 kb on + strand at 221.255 kb on + strand at 221.256 kb on - strand at 221.256 kb on - strand at 221.257 kb on + strand at 221.258 kb on - strand at 221.258 kb on - strand at 221.389 kb on + strand, within Pf6N2E2_168 at 221.389 kb on + strand, within Pf6N2E2_168 at 221.429 kb on + strand, within Pf6N2E2_168 at 221.429 kb on + strand, within Pf6N2E2_168 at 221.429 kb on + strand, within Pf6N2E2_168 at 221.430 kb on - strand, within Pf6N2E2_168 at 221.430 kb on - strand, within Pf6N2E2_168 at 221.430 kb on - strand, within Pf6N2E2_168 at 221.436 kb on + strand, within Pf6N2E2_168 at 221.436 kb on + strand, within Pf6N2E2_168 at 221.437 kb on - strand, within Pf6N2E2_168 at 221.493 kb on + strand at 221.493 kb on + strand at 221.494 kb on - strand at 221.517 kb on + strand at 221.517 kb on + strand at 221.517 kb on + strand at 221.518 kb on - strand at 221.518 kb on - strand at 221.518 kb on - strand at 221.518 kb on - strand at 221.527 kb on + strand at 221.528 kb on - strand at 221.561 kb on + strand at 221.562 kb on - strand at 221.562 kb on - strand at 221.702 kb on + strand, within Pf6N2E2_169 at 221.703 kb on - strand, within Pf6N2E2_169 at 221.736 kb on + strand, within Pf6N2E2_169 at 221.737 kb on - strand, within Pf6N2E2_169 at 221.737 kb on - strand, within Pf6N2E2_169 at 221.737 kb on - strand, within Pf6N2E2_169 at 221.737 kb on - strand, within Pf6N2E2_169 at 221.817 kb on + strand, within Pf6N2E2_169 at 221.865 kb on + strand, within Pf6N2E2_169 at 221.865 kb on + strand, within Pf6N2E2_169 at 221.865 kb on + strand, within Pf6N2E2_169 at 221.865 kb on + strand, within Pf6N2E2_169 at 221.865 kb on + strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.882 kb on + strand, within Pf6N2E2_169 at 221.882 kb on + strand, within Pf6N2E2_169 at 221.882 kb on + strand, within Pf6N2E2_169 at 221.883 kb on - strand, within Pf6N2E2_169 at 221.883 kb on - strand, within Pf6N2E2_169 at 221.907 kb on + strand at 221.907 kb on + strand at 221.920 kb on - strand at 222.063 kb on + strand at 222.063 kb on + strand at 222.063 kb on + strand at 222.064 kb on - strand at 222.113 kb on + strand at 222.113 kb on + strand at 222.113 kb on + strand at 222.114 kb on - strand at 222.114 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 5mM remove 219,377 - Pf6N2E2_165 0.55 +0.3 219,395 + Pf6N2E2_165 0.57 +0.3 219,421 + Pf6N2E2_165 0.60 +0.6 219,421 + Pf6N2E2_165 0.60 +0.7 219,421 + Pf6N2E2_165 0.60 +0.1 219,421 + Pf6N2E2_165 0.60 -1.0 219,765 + +0.3 219,766 - -0.6 219,766 - -2.2 219,766 - -1.3 220,184 - -0.2 220,184 - +1.0 220,184 - -0.3 220,226 - -0.3 220,393 - Pf6N2E2_167 0.17 -1.1 220,393 - Pf6N2E2_167 0.17 +0.1 220,393 - Pf6N2E2_167 0.17 -0.2 220,393 - Pf6N2E2_167 0.17 +0.2 220,495 + Pf6N2E2_167 0.27 -0.4 220,496 - Pf6N2E2_167 0.27 -2.3 220,580 + Pf6N2E2_167 0.35 +1.1 220,581 - Pf6N2E2_167 0.35 -2.2 220,581 - Pf6N2E2_167 0.35 +1.7 220,624 + Pf6N2E2_167 0.40 -1.1 220,785 + Pf6N2E2_167 0.55 -0.8 220,785 + Pf6N2E2_167 0.55 -0.7 220,867 + Pf6N2E2_167 0.63 -1.5 220,978 + Pf6N2E2_167 0.74 +0.6 220,978 + Pf6N2E2_167 0.74 +1.2 220,979 - Pf6N2E2_167 0.74 +0.6 220,979 - Pf6N2E2_167 0.74 -3.2 220,979 - Pf6N2E2_167 0.74 +0.7 220,979 - Pf6N2E2_167 0.74 -1.9 220,979 - Pf6N2E2_167 0.74 +0.1 220,979 - Pf6N2E2_167 0.74 +0.1 221,002 + Pf6N2E2_167 0.76 -0.9 221,002 + Pf6N2E2_167 0.76 +0.1 221,144 - +1.7 221,144 - +0.7 221,255 + +0.8 221,255 + +0.0 221,256 - -1.5 221,256 - -1.5 221,257 + +0.2 221,258 - +1.1 221,258 - -0.4 221,389 + Pf6N2E2_168 0.48 +0.6 221,389 + Pf6N2E2_168 0.48 -1.5 221,429 + Pf6N2E2_168 0.69 -0.6 221,429 + Pf6N2E2_168 0.69 +0.7 221,429 + Pf6N2E2_168 0.69 +0.1 221,430 - Pf6N2E2_168 0.69 -0.5 221,430 - Pf6N2E2_168 0.69 -0.6 221,430 - Pf6N2E2_168 0.69 -1.1 221,436 + Pf6N2E2_168 0.72 +1.0 221,436 + Pf6N2E2_168 0.72 +0.4 221,437 - Pf6N2E2_168 0.73 -0.8 221,493 + -1.5 221,493 + -1.1 221,494 - -0.0 221,517 + +0.5 221,517 + -1.0 221,517 + -0.9 221,518 - +1.3 221,518 - -0.2 221,518 - -0.3 221,518 - +1.1 221,527 + +0.7 221,528 - -2.0 221,561 + -0.4 221,562 - +0.9 221,562 - +0.8 221,702 + Pf6N2E2_169 0.27 +0.7 221,703 - Pf6N2E2_169 0.27 +0.2 221,736 + Pf6N2E2_169 0.38 -0.8 221,737 - Pf6N2E2_169 0.39 +0.7 221,737 - Pf6N2E2_169 0.39 +0.4 221,737 - Pf6N2E2_169 0.39 +1.3 221,737 - Pf6N2E2_169 0.39 -0.8 221,817 + Pf6N2E2_169 0.65 -0.6 221,865 + Pf6N2E2_169 0.80 +0.9 221,865 + Pf6N2E2_169 0.80 -0.3 221,865 + Pf6N2E2_169 0.80 -0.8 221,865 + Pf6N2E2_169 0.80 -2.0 221,865 + Pf6N2E2_169 0.80 +0.4 221,866 - Pf6N2E2_169 0.81 -1.1 221,866 - Pf6N2E2_169 0.81 +0.1 221,866 - Pf6N2E2_169 0.81 +0.2 221,866 - Pf6N2E2_169 0.81 +1.7 221,866 - Pf6N2E2_169 0.81 +0.7 221,866 - Pf6N2E2_169 0.81 +1.7 221,866 - Pf6N2E2_169 0.81 +0.5 221,866 - Pf6N2E2_169 0.81 +1.9 221,882 + Pf6N2E2_169 0.86 -0.3 221,882 + Pf6N2E2_169 0.86 -1.5 221,882 + Pf6N2E2_169 0.86 +1.4 221,883 - Pf6N2E2_169 0.86 -0.2 221,883 - Pf6N2E2_169 0.86 +1.6 221,907 + +0.2 221,907 + -0.1 221,920 - +0.6 222,063 + +0.6 222,063 + +0.0 222,063 + +0.6 222,064 - +2.7 222,113 + +0.4 222,113 + +1.7 222,113 + -0.9 222,114 - +0.0 222,114 - +0.5
Or see this region's nucleotide sequence