Experiment: nitrogen source nitrate 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_425 and Pf6N2E2_426 are separated by 4 nucleotides Pf6N2E2_426 and Pf6N2E2_427 are separated by 161 nucleotides Pf6N2E2_427 and Pf6N2E2_428 are separated by 125 nucleotides Pf6N2E2_428 and Pf6N2E2_429 are separated by 117 nucleotides
Pf6N2E2_425 - FIG00957304: hypothetical protein, at 494,309 to 494,842
_425
Pf6N2E2_426 - Arsenate reductase (EC 1.20.4.1), at 494,847 to 495,302
_426
Pf6N2E2_427 - Conidiation-specific protein 10, at 495,464 to 495,643
_427
Pf6N2E2_428 - Protease subunit of ATP-dependent Clp proteases, at 495,769 to 496,332
_428
Pf6N2E2_429 - Hypothetical protein PA2244 (similar to DNA topoisomerase IB, but possibly involved in glycosyl-transfer), at 496,450 to 497,505
_429
Position (kb) 495
496
497 Strain fitness (log2 ratio) -2
-1
0
1
2 at 494.828 kb on + strand at 495.046 kb on + strand, within Pf6N2E2_426 at 495.047 kb on - strand, within Pf6N2E2_426 at 495.047 kb on - strand, within Pf6N2E2_426 at 495.047 kb on - strand, within Pf6N2E2_426 at 495.147 kb on + strand, within Pf6N2E2_426 at 495.147 kb on + strand, within Pf6N2E2_426 at 495.148 kb on - strand, within Pf6N2E2_426 at 495.148 kb on - strand, within Pf6N2E2_426 at 495.148 kb on - strand, within Pf6N2E2_426 at 495.148 kb on - strand, within Pf6N2E2_426 at 495.358 kb on + strand at 495.358 kb on + strand at 495.359 kb on - strand at 495.359 kb on - strand at 495.359 kb on - strand at 495.360 kb on + strand at 495.377 kb on - strand at 495.378 kb on + strand at 495.689 kb on - strand at 495.689 kb on - strand at 495.770 kb on + strand at 495.771 kb on - strand at 495.771 kb on - strand at 495.818 kb on - strand at 495.906 kb on + strand, within Pf6N2E2_428 at 495.944 kb on + strand, within Pf6N2E2_428 at 495.944 kb on + strand, within Pf6N2E2_428 at 495.944 kb on + strand, within Pf6N2E2_428 at 495.990 kb on - strand, within Pf6N2E2_428 at 495.990 kb on - strand, within Pf6N2E2_428 at 496.031 kb on + strand, within Pf6N2E2_428 at 496.032 kb on - strand, within Pf6N2E2_428 at 496.097 kb on + strand, within Pf6N2E2_428 at 496.098 kb on - strand, within Pf6N2E2_428 at 496.098 kb on - strand, within Pf6N2E2_428 at 496.098 kb on - strand, within Pf6N2E2_428 at 496.098 kb on - strand, within Pf6N2E2_428 at 496.098 kb on - strand, within Pf6N2E2_428 at 496.098 kb on - strand, within Pf6N2E2_428 at 496.144 kb on + strand, within Pf6N2E2_428 at 496.145 kb on - strand, within Pf6N2E2_428 at 496.145 kb on - strand, within Pf6N2E2_428 at 496.175 kb on + strand, within Pf6N2E2_428 at 496.175 kb on + strand, within Pf6N2E2_428 at 496.266 kb on + strand, within Pf6N2E2_428 at 496.387 kb on + strand at 496.388 kb on - strand at 496.508 kb on - strand at 496.542 kb on + strand at 496.542 kb on + strand at 496.543 kb on - strand at 496.543 kb on - strand at 496.613 kb on + strand, within Pf6N2E2_429 at 496.613 kb on + strand, within Pf6N2E2_429 at 496.614 kb on - strand, within Pf6N2E2_429 at 496.614 kb on - strand, within Pf6N2E2_429 at 497.027 kb on + strand, within Pf6N2E2_429 at 497.027 kb on + strand, within Pf6N2E2_429 at 497.028 kb on - strand, within Pf6N2E2_429 at 497.028 kb on - strand, within Pf6N2E2_429 at 497.201 kb on + strand, within Pf6N2E2_429 at 497.202 kb on - strand, within Pf6N2E2_429 at 497.202 kb on - strand, within Pf6N2E2_429 at 497.202 kb on - strand, within Pf6N2E2_429 at 497.202 kb on - strand, within Pf6N2E2_429 at 497.202 kb on - strand, within Pf6N2E2_429 at 497.247 kb on - strand, within Pf6N2E2_429 at 497.322 kb on + strand, within Pf6N2E2_429 at 497.324 kb on + strand, within Pf6N2E2_429 at 497.324 kb on + strand, within Pf6N2E2_429 at 497.324 kb on + strand, within Pf6N2E2_429
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 5mM remove 494,828 + +0.0 495,046 + Pf6N2E2_426 0.44 +2.0 495,047 - Pf6N2E2_426 0.44 -1.0 495,047 - Pf6N2E2_426 0.44 -0.4 495,047 - Pf6N2E2_426 0.44 +1.7 495,147 + Pf6N2E2_426 0.66 +1.7 495,147 + Pf6N2E2_426 0.66 -0.2 495,148 - Pf6N2E2_426 0.66 -0.0 495,148 - Pf6N2E2_426 0.66 -0.2 495,148 - Pf6N2E2_426 0.66 +0.3 495,148 - Pf6N2E2_426 0.66 -1.7 495,358 + +0.1 495,358 + +0.5 495,359 - +0.9 495,359 - -1.6 495,359 - -0.2 495,360 + +0.2 495,377 - +0.3 495,378 + -0.9 495,689 - -0.1 495,689 - +0.1 495,770 + +1.7 495,771 - -0.3 495,771 - +0.5 495,818 - +0.1 495,906 + Pf6N2E2_428 0.24 +0.3 495,944 + Pf6N2E2_428 0.31 +1.7 495,944 + Pf6N2E2_428 0.31 +0.1 495,944 + Pf6N2E2_428 0.31 -1.8 495,990 - Pf6N2E2_428 0.39 +1.7 495,990 - Pf6N2E2_428 0.39 -1.1 496,031 + Pf6N2E2_428 0.46 +0.2 496,032 - Pf6N2E2_428 0.47 -0.1 496,097 + Pf6N2E2_428 0.58 -1.2 496,098 - Pf6N2E2_428 0.58 -1.6 496,098 - Pf6N2E2_428 0.58 -0.1 496,098 - Pf6N2E2_428 0.58 -1.2 496,098 - Pf6N2E2_428 0.58 -0.5 496,098 - Pf6N2E2_428 0.58 +0.2 496,098 - Pf6N2E2_428 0.58 -0.5 496,144 + Pf6N2E2_428 0.66 +0.1 496,145 - Pf6N2E2_428 0.67 -0.7 496,145 - Pf6N2E2_428 0.67 +1.7 496,175 + Pf6N2E2_428 0.72 -0.7 496,175 + Pf6N2E2_428 0.72 -1.6 496,266 + Pf6N2E2_428 0.88 -0.3 496,387 + -0.4 496,388 - +1.8 496,508 - +0.7 496,542 + -0.3 496,542 + +0.5 496,543 - -1.8 496,543 - +2.1 496,613 + Pf6N2E2_429 0.15 -2.3 496,613 + Pf6N2E2_429 0.15 -1.4 496,614 - Pf6N2E2_429 0.16 +0.3 496,614 - Pf6N2E2_429 0.16 -1.8 497,027 + Pf6N2E2_429 0.55 -0.9 497,027 + Pf6N2E2_429 0.55 -0.6 497,028 - Pf6N2E2_429 0.55 +0.7 497,028 - Pf6N2E2_429 0.55 -1.2 497,201 + Pf6N2E2_429 0.71 -1.4 497,202 - Pf6N2E2_429 0.71 -0.0 497,202 - Pf6N2E2_429 0.71 +0.7 497,202 - Pf6N2E2_429 0.71 -0.8 497,202 - Pf6N2E2_429 0.71 +0.5 497,202 - Pf6N2E2_429 0.71 +0.5 497,247 - Pf6N2E2_429 0.75 -0.7 497,322 + Pf6N2E2_429 0.83 -0.6 497,324 + Pf6N2E2_429 0.83 -1.3 497,324 + Pf6N2E2_429 0.83 +0.8 497,324 + Pf6N2E2_429 0.83 -0.6
Or see this region's nucleotide sequence