Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2705 and Pf6N2E2_2706 are separated by 102 nucleotides Pf6N2E2_2706 and Pf6N2E2_2707 overlap by 4 nucleotides Pf6N2E2_2707 and Pf6N2E2_2708 are separated by 8 nucleotides
Pf6N2E2_2705 - Thioredoxin reductase (EC 1.8.1.9), at 3,197,959 to 3,199,137
_2705
Pf6N2E2_2706 - Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54), at 3,199,240 to 3,200,160
_2706
Pf6N2E2_2707 - Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54), at 3,200,157 to 3,200,849
_2707
Pf6N2E2_2708 - Lactam utilization protein LamB, at 3,200,858 to 3,201,610
_2708
Position (kb)
3199
3200
3201 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3198.469 kb on + strand, within Pf6N2E2_2705 at 3198.469 kb on + strand, within Pf6N2E2_2705 at 3198.470 kb on - strand, within Pf6N2E2_2705 at 3198.757 kb on + strand, within Pf6N2E2_2705 at 3198.757 kb on + strand, within Pf6N2E2_2705 at 3198.757 kb on + strand, within Pf6N2E2_2705 at 3198.758 kb on - strand, within Pf6N2E2_2705 at 3198.758 kb on - strand, within Pf6N2E2_2705 at 3198.758 kb on - strand, within Pf6N2E2_2705 at 3198.758 kb on - strand, within Pf6N2E2_2705 at 3198.763 kb on + strand, within Pf6N2E2_2705 at 3198.763 kb on + strand, within Pf6N2E2_2705 at 3198.763 kb on + strand, within Pf6N2E2_2705 at 3198.763 kb on + strand, within Pf6N2E2_2705 at 3198.763 kb on + strand, within Pf6N2E2_2705 at 3198.763 kb on + strand, within Pf6N2E2_2705 at 3198.763 kb on + strand, within Pf6N2E2_2705 at 3198.763 kb on + strand, within Pf6N2E2_2705 at 3198.781 kb on + strand, within Pf6N2E2_2705 at 3198.782 kb on - strand, within Pf6N2E2_2705 at 3198.814 kb on - strand, within Pf6N2E2_2705 at 3198.853 kb on + strand, within Pf6N2E2_2705 at 3198.853 kb on + strand, within Pf6N2E2_2705 at 3198.853 kb on + strand, within Pf6N2E2_2705 at 3198.853 kb on + strand, within Pf6N2E2_2705 at 3198.854 kb on - strand, within Pf6N2E2_2705 at 3198.854 kb on - strand, within Pf6N2E2_2705 at 3198.854 kb on - strand, within Pf6N2E2_2705 at 3198.854 kb on - strand, within Pf6N2E2_2705 at 3198.854 kb on - strand, within Pf6N2E2_2705 at 3198.866 kb on - strand, within Pf6N2E2_2705 at 3198.958 kb on + strand, within Pf6N2E2_2705 at 3198.958 kb on + strand, within Pf6N2E2_2705 at 3198.958 kb on + strand, within Pf6N2E2_2705 at 3198.958 kb on + strand, within Pf6N2E2_2705 at 3198.959 kb on - strand, within Pf6N2E2_2705 at 3198.959 kb on - strand, within Pf6N2E2_2705 at 3198.959 kb on - strand, within Pf6N2E2_2705 at 3198.988 kb on + strand, within Pf6N2E2_2705 at 3198.988 kb on + strand, within Pf6N2E2_2705 at 3198.989 kb on - strand at 3198.989 kb on - strand, within Pf6N2E2_2705 at 3198.989 kb on - strand, within Pf6N2E2_2705 at 3199.036 kb on + strand at 3199.037 kb on - strand at 3199.039 kb on - strand at 3199.085 kb on - strand at 3199.085 kb on - strand at 3199.316 kb on - strand at 3199.493 kb on + strand, within Pf6N2E2_2706 at 3199.494 kb on - strand, within Pf6N2E2_2706 at 3199.694 kb on + strand, within Pf6N2E2_2706 at 3199.695 kb on - strand, within Pf6N2E2_2706 at 3199.975 kb on - strand, within Pf6N2E2_2706 at 3200.483 kb on - strand, within Pf6N2E2_2707 at 3200.483 kb on - strand, within Pf6N2E2_2707 at 3200.576 kb on - strand, within Pf6N2E2_2707 at 3200.690 kb on - strand, within Pf6N2E2_2707 at 3200.690 kb on - strand, within Pf6N2E2_2707 at 3200.709 kb on - strand, within Pf6N2E2_2707 at 3200.710 kb on + strand, within Pf6N2E2_2707 at 3200.954 kb on - strand, within Pf6N2E2_2708 at 3200.954 kb on - strand, within Pf6N2E2_2708 at 3200.954 kb on - strand, within Pf6N2E2_2708 at 3201.090 kb on + strand, within Pf6N2E2_2708 at 3201.091 kb on - strand, within Pf6N2E2_2708
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 3,198,469 + Pf6N2E2_2705 0.43 -2.1 3,198,469 + Pf6N2E2_2705 0.43 -0.7 3,198,470 - Pf6N2E2_2705 0.43 +0.7 3,198,757 + Pf6N2E2_2705 0.68 -1.7 3,198,757 + Pf6N2E2_2705 0.68 -0.8 3,198,757 + Pf6N2E2_2705 0.68 -0.4 3,198,758 - Pf6N2E2_2705 0.68 -0.9 3,198,758 - Pf6N2E2_2705 0.68 +0.2 3,198,758 - Pf6N2E2_2705 0.68 -3.5 3,198,758 - Pf6N2E2_2705 0.68 -0.2 3,198,763 + Pf6N2E2_2705 0.68 +1.0 3,198,763 + Pf6N2E2_2705 0.68 -0.4 3,198,763 + Pf6N2E2_2705 0.68 +0.3 3,198,763 + Pf6N2E2_2705 0.68 -0.8 3,198,763 + Pf6N2E2_2705 0.68 -1.4 3,198,763 + Pf6N2E2_2705 0.68 -0.5 3,198,763 + Pf6N2E2_2705 0.68 -0.5 3,198,763 + Pf6N2E2_2705 0.68 +0.2 3,198,781 + Pf6N2E2_2705 0.70 -1.5 3,198,782 - Pf6N2E2_2705 0.70 +0.8 3,198,814 - Pf6N2E2_2705 0.73 -0.2 3,198,853 + Pf6N2E2_2705 0.76 +0.2 3,198,853 + Pf6N2E2_2705 0.76 +1.8 3,198,853 + Pf6N2E2_2705 0.76 -0.1 3,198,853 + Pf6N2E2_2705 0.76 -0.8 3,198,854 - Pf6N2E2_2705 0.76 -0.5 3,198,854 - Pf6N2E2_2705 0.76 +0.5 3,198,854 - Pf6N2E2_2705 0.76 +1.8 3,198,854 - Pf6N2E2_2705 0.76 -0.2 3,198,854 - Pf6N2E2_2705 0.76 +0.5 3,198,866 - Pf6N2E2_2705 0.77 -1.0 3,198,958 + Pf6N2E2_2705 0.85 +0.8 3,198,958 + Pf6N2E2_2705 0.85 -0.9 3,198,958 + Pf6N2E2_2705 0.85 +0.5 3,198,958 + Pf6N2E2_2705 0.85 +0.1 3,198,959 - Pf6N2E2_2705 0.85 -1.0 3,198,959 - Pf6N2E2_2705 0.85 +0.6 3,198,959 - Pf6N2E2_2705 0.85 +1.6 3,198,988 + Pf6N2E2_2705 0.87 -0.3 3,198,988 + Pf6N2E2_2705 0.87 +0.8 3,198,989 - -1.0 3,198,989 - Pf6N2E2_2705 0.87 -0.5 3,198,989 - Pf6N2E2_2705 0.87 +0.1 3,199,036 + -1.9 3,199,037 - -0.5 3,199,039 - -0.2 3,199,085 - -0.4 3,199,085 - -0.7 3,199,316 - -3.6 3,199,493 + Pf6N2E2_2706 0.27 +1.8 3,199,494 - Pf6N2E2_2706 0.28 -0.2 3,199,694 + Pf6N2E2_2706 0.49 -0.2 3,199,695 - Pf6N2E2_2706 0.49 -1.5 3,199,975 - Pf6N2E2_2706 0.80 -0.8 3,200,483 - Pf6N2E2_2707 0.47 +0.2 3,200,483 - Pf6N2E2_2707 0.47 -0.4 3,200,576 - Pf6N2E2_2707 0.60 -0.2 3,200,690 - Pf6N2E2_2707 0.77 -3.5 3,200,690 - Pf6N2E2_2707 0.77 -5.3 3,200,709 - Pf6N2E2_2707 0.80 -0.9 3,200,710 + Pf6N2E2_2707 0.80 +0.2 3,200,954 - Pf6N2E2_2708 0.13 -0.1 3,200,954 - Pf6N2E2_2708 0.13 +0.0 3,200,954 - Pf6N2E2_2708 0.13 -2.4 3,201,090 + Pf6N2E2_2708 0.31 +1.8 3,201,091 - Pf6N2E2_2708 0.31 +1.8
Or see this region's nucleotide sequence