Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_225 and Pf6N2E2_226 are separated by 74 nucleotides Pf6N2E2_226 and Pf6N2E2_227 are separated by 22 nucleotides
Pf6N2E2_225 - Putative benzaldehyde dehydrogenase oxidoreductase protein (EC 1.2.1.28), at 286,305 to 287,783
_225
Pf6N2E2_226 - 2,3-dihydroxybiphenyl 1,2-dioxygenase, at 287,858 to 288,706
_226
Pf6N2E2_227 - 3-(3-hydroxy-phenyl)propionate hydroxylase (EC 1.14.13.-), at 288,729 to 290,375
_227
Position (kb) 287
288
289 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 286.864 kb on + strand, within Pf6N2E2_225 at 286.864 kb on + strand, within Pf6N2E2_225 at 286.865 kb on - strand, within Pf6N2E2_225 at 287.377 kb on + strand, within Pf6N2E2_225 at 287.377 kb on + strand, within Pf6N2E2_225 at 287.377 kb on + strand, within Pf6N2E2_225 at 287.617 kb on + strand, within Pf6N2E2_225 at 287.618 kb on - strand, within Pf6N2E2_225 at 287.737 kb on + strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.792 kb on - strand at 287.821 kb on - strand at 287.821 kb on - strand at 287.898 kb on + strand at 287.898 kb on + strand at 287.898 kb on + strand at 287.899 kb on - strand at 287.899 kb on - strand at 287.899 kb on - strand at 287.899 kb on - strand at 287.899 kb on - strand at 288.109 kb on - strand, within Pf6N2E2_226 at 288.256 kb on - strand, within Pf6N2E2_226 at 288.256 kb on - strand, within Pf6N2E2_226 at 288.362 kb on + strand, within Pf6N2E2_226 at 288.363 kb on - strand, within Pf6N2E2_226 at 288.363 kb on - strand, within Pf6N2E2_226 at 288.666 kb on + strand at 288.730 kb on + strand at 289.034 kb on + strand, within Pf6N2E2_227 at 289.035 kb on - strand, within Pf6N2E2_227 at 289.237 kb on + strand, within Pf6N2E2_227 at 289.489 kb on + strand, within Pf6N2E2_227 at 289.489 kb on + strand, within Pf6N2E2_227 at 289.574 kb on - strand, within Pf6N2E2_227 at 289.574 kb on - strand, within Pf6N2E2_227 at 289.574 kb on - strand, within Pf6N2E2_227 at 289.574 kb on - strand, within Pf6N2E2_227
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 286,864 + Pf6N2E2_225 0.38 -1.4 286,864 + Pf6N2E2_225 0.38 +1.7 286,865 - Pf6N2E2_225 0.38 -1.1 287,377 + Pf6N2E2_225 0.72 -0.3 287,377 + Pf6N2E2_225 0.72 -0.1 287,377 + Pf6N2E2_225 0.72 +1.7 287,617 + Pf6N2E2_225 0.89 -0.3 287,618 - Pf6N2E2_225 0.89 -0.6 287,737 + -0.3 287,738 - +0.1 287,738 - +0.6 287,738 - -1.0 287,738 - +0.4 287,738 - +0.8 287,792 - +0.2 287,821 - +0.2 287,821 - +1.7 287,898 + -0.4 287,898 + +1.7 287,898 + +0.2 287,899 - -0.8 287,899 - +1.7 287,899 - +0.1 287,899 - +0.3 287,899 - -1.3 288,109 - Pf6N2E2_226 0.30 +0.7 288,256 - Pf6N2E2_226 0.47 -1.3 288,256 - Pf6N2E2_226 0.47 -0.0 288,362 + Pf6N2E2_226 0.59 -1.2 288,363 - Pf6N2E2_226 0.59 -0.2 288,363 - Pf6N2E2_226 0.59 -3.0 288,666 + +0.3 288,730 + +1.7 289,034 + Pf6N2E2_227 0.19 -0.6 289,035 - Pf6N2E2_227 0.19 +0.1 289,237 + Pf6N2E2_227 0.31 -0.2 289,489 + Pf6N2E2_227 0.46 -0.5 289,489 + Pf6N2E2_227 0.46 -1.3 289,574 - Pf6N2E2_227 0.51 +0.3 289,574 - Pf6N2E2_227 0.51 -0.1 289,574 - Pf6N2E2_227 0.51 -1.3 289,574 - Pf6N2E2_227 0.51 -1.1
Or see this region's nucleotide sequence