Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1918 and Pf6N2E2_1919 are separated by 205 nucleotides Pf6N2E2_1919 and Pf6N2E2_1920 are separated by 87 nucleotides Pf6N2E2_1920 and Pf6N2E2_1921 are separated by 22 nucleotides Pf6N2E2_1921 and Pf6N2E2_1922 overlap by 4 nucleotides
Pf6N2E2_1918 - Major facilitator family transporter, at 2,271,740 to 2,272,900
_1918
Pf6N2E2_1919 - Aldehyde dehydrogenase (EC 1.2.1.3), at 2,273,106 to 2,274,578
_1919
Pf6N2E2_1920 - Rubredoxin, at 2,274,666 to 2,274,872
_1920
Pf6N2E2_1921 - hypothetical protein, at 2,274,895 to 2,275,032
_1921
Pf6N2E2_1922 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,275,029 to 2,277,140
_1922
Position (kb)
2273
2274
2275 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2272.128 kb on - strand, within Pf6N2E2_1918 at 2272.216 kb on - strand, within Pf6N2E2_1918 at 2272.216 kb on - strand, within Pf6N2E2_1918 at 2272.216 kb on - strand, within Pf6N2E2_1918 at 2272.221 kb on + strand, within Pf6N2E2_1918 at 2272.222 kb on - strand, within Pf6N2E2_1918 at 2272.222 kb on - strand, within Pf6N2E2_1918 at 2272.222 kb on - strand, within Pf6N2E2_1918 at 2272.222 kb on - strand, within Pf6N2E2_1918 at 2272.231 kb on - strand, within Pf6N2E2_1918 at 2272.398 kb on + strand, within Pf6N2E2_1918 at 2272.436 kb on - strand at 2272.605 kb on + strand, within Pf6N2E2_1918 at 2272.628 kb on - strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.665 kb on + strand, within Pf6N2E2_1918 at 2272.666 kb on - strand, within Pf6N2E2_1918 at 2272.666 kb on - strand, within Pf6N2E2_1918 at 2272.666 kb on - strand, within Pf6N2E2_1918 at 2272.666 kb on - strand, within Pf6N2E2_1918 at 2272.737 kb on + strand, within Pf6N2E2_1918 at 2272.737 kb on + strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.738 kb on - strand, within Pf6N2E2_1918 at 2272.847 kb on - strand at 2272.973 kb on + strand at 2272.973 kb on + strand at 2273.018 kb on + strand at 2273.019 kb on - strand at 2273.127 kb on + strand at 2273.149 kb on + strand at 2273.149 kb on + strand at 2273.149 kb on + strand at 2273.150 kb on - strand at 2273.334 kb on + strand, within Pf6N2E2_1919 at 2273.431 kb on - strand, within Pf6N2E2_1919 at 2273.431 kb on - strand, within Pf6N2E2_1919 at 2273.431 kb on - strand, within Pf6N2E2_1919 at 2273.482 kb on - strand, within Pf6N2E2_1919 at 2273.482 kb on - strand, within Pf6N2E2_1919 at 2273.617 kb on - strand, within Pf6N2E2_1919 at 2273.659 kb on + strand, within Pf6N2E2_1919 at 2273.660 kb on - strand, within Pf6N2E2_1919 at 2273.664 kb on - strand, within Pf6N2E2_1919 at 2273.664 kb on - strand, within Pf6N2E2_1919 at 2273.995 kb on - strand, within Pf6N2E2_1919 at 2274.259 kb on - strand, within Pf6N2E2_1919 at 2274.259 kb on - strand, within Pf6N2E2_1919 at 2274.341 kb on + strand, within Pf6N2E2_1919 at 2274.341 kb on + strand, within Pf6N2E2_1919 at 2274.341 kb on + strand, within Pf6N2E2_1919 at 2274.342 kb on - strand, within Pf6N2E2_1919 at 2274.344 kb on + strand, within Pf6N2E2_1919 at 2274.345 kb on - strand, within Pf6N2E2_1919 at 2274.345 kb on - strand, within Pf6N2E2_1919 at 2274.345 kb on - strand, within Pf6N2E2_1919 at 2274.557 kb on + strand at 2274.558 kb on - strand at 2274.558 kb on - strand at 2274.558 kb on - strand at 2274.664 kb on - strand at 2274.664 kb on - strand at 2274.760 kb on - strand, within Pf6N2E2_1920 at 2274.760 kb on - strand, within Pf6N2E2_1920 at 2274.777 kb on - strand, within Pf6N2E2_1920 at 2274.777 kb on - strand, within Pf6N2E2_1920 at 2274.896 kb on + strand at 2274.935 kb on + strand, within Pf6N2E2_1921 at 2275.033 kb on - strand at 2275.033 kb on - strand at 2275.033 kb on - strand at 2275.094 kb on + strand at 2275.115 kb on - strand at 2275.178 kb on - strand at 2275.216 kb on + strand at 2275.257 kb on - strand, within Pf6N2E2_1922 at 2275.257 kb on - strand, within Pf6N2E2_1922 at 2275.257 kb on - strand, within Pf6N2E2_1922 at 2275.299 kb on + strand, within Pf6N2E2_1922 at 2275.299 kb on + strand, within Pf6N2E2_1922 at 2275.335 kb on - strand, within Pf6N2E2_1922 at 2275.430 kb on - strand, within Pf6N2E2_1922 at 2275.501 kb on + strand, within Pf6N2E2_1922 at 2275.502 kb on - strand, within Pf6N2E2_1922 at 2275.541 kb on - strand, within Pf6N2E2_1922
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 2,272,128 - Pf6N2E2_1918 0.33 -0.0 2,272,216 - Pf6N2E2_1918 0.41 -2.7 2,272,216 - Pf6N2E2_1918 0.41 -0.5 2,272,216 - Pf6N2E2_1918 0.41 +0.4 2,272,221 + Pf6N2E2_1918 0.41 +0.5 2,272,222 - Pf6N2E2_1918 0.42 +0.7 2,272,222 - Pf6N2E2_1918 0.42 +0.2 2,272,222 - Pf6N2E2_1918 0.42 -2.3 2,272,222 - Pf6N2E2_1918 0.42 -0.3 2,272,231 - Pf6N2E2_1918 0.42 +1.7 2,272,398 + Pf6N2E2_1918 0.57 -1.6 2,272,436 - -1.7 2,272,605 + Pf6N2E2_1918 0.75 +1.7 2,272,628 - Pf6N2E2_1918 0.76 -0.3 2,272,665 + Pf6N2E2_1918 0.80 -1.7 2,272,665 + Pf6N2E2_1918 0.80 -2.0 2,272,665 + Pf6N2E2_1918 0.80 -0.2 2,272,665 + Pf6N2E2_1918 0.80 -0.0 2,272,665 + Pf6N2E2_1918 0.80 -0.9 2,272,666 - Pf6N2E2_1918 0.80 +1.7 2,272,666 - Pf6N2E2_1918 0.80 -1.4 2,272,666 - Pf6N2E2_1918 0.80 +1.2 2,272,666 - Pf6N2E2_1918 0.80 -2.3 2,272,737 + Pf6N2E2_1918 0.86 -0.3 2,272,737 + Pf6N2E2_1918 0.86 +0.5 2,272,738 - Pf6N2E2_1918 0.86 -0.3 2,272,738 - Pf6N2E2_1918 0.86 +0.2 2,272,738 - Pf6N2E2_1918 0.86 +1.7 2,272,738 - Pf6N2E2_1918 0.86 +0.3 2,272,738 - Pf6N2E2_1918 0.86 +1.2 2,272,738 - Pf6N2E2_1918 0.86 -0.2 2,272,738 - Pf6N2E2_1918 0.86 -0.8 2,272,847 - +1.7 2,272,973 + +1.7 2,272,973 + +0.2 2,273,018 + +0.3 2,273,019 - -0.0 2,273,127 + +1.7 2,273,149 + +1.4 2,273,149 + +0.7 2,273,149 + +0.4 2,273,150 - -0.7 2,273,334 + Pf6N2E2_1919 0.15 +0.7 2,273,431 - Pf6N2E2_1919 0.22 -1.1 2,273,431 - Pf6N2E2_1919 0.22 +2.3 2,273,431 - Pf6N2E2_1919 0.22 -1.1 2,273,482 - Pf6N2E2_1919 0.26 -0.5 2,273,482 - Pf6N2E2_1919 0.26 +1.7 2,273,617 - Pf6N2E2_1919 0.35 -0.8 2,273,659 + Pf6N2E2_1919 0.38 -1.3 2,273,660 - Pf6N2E2_1919 0.38 -0.5 2,273,664 - Pf6N2E2_1919 0.38 +1.7 2,273,664 - Pf6N2E2_1919 0.38 -1.7 2,273,995 - Pf6N2E2_1919 0.60 -1.1 2,274,259 - Pf6N2E2_1919 0.78 -1.3 2,274,259 - Pf6N2E2_1919 0.78 +1.7 2,274,341 + Pf6N2E2_1919 0.84 +0.6 2,274,341 + Pf6N2E2_1919 0.84 -1.0 2,274,341 + Pf6N2E2_1919 0.84 +0.0 2,274,342 - Pf6N2E2_1919 0.84 -2.1 2,274,344 + Pf6N2E2_1919 0.84 -0.3 2,274,345 - Pf6N2E2_1919 0.84 +0.1 2,274,345 - Pf6N2E2_1919 0.84 +0.7 2,274,345 - Pf6N2E2_1919 0.84 +0.7 2,274,557 + +1.5 2,274,558 - +1.7 2,274,558 - +1.7 2,274,558 - -1.6 2,274,664 - -0.3 2,274,664 - -0.3 2,274,760 - Pf6N2E2_1920 0.45 -2.1 2,274,760 - Pf6N2E2_1920 0.45 +0.1 2,274,777 - Pf6N2E2_1920 0.54 -1.8 2,274,777 - Pf6N2E2_1920 0.54 -2.4 2,274,896 + +1.7 2,274,935 + Pf6N2E2_1921 0.29 -2.0 2,275,033 - +0.1 2,275,033 - +0.2 2,275,033 - -0.9 2,275,094 + +0.0 2,275,115 - -0.4 2,275,178 - -0.8 2,275,216 + +0.0 2,275,257 - Pf6N2E2_1922 0.11 +1.9 2,275,257 - Pf6N2E2_1922 0.11 -0.8 2,275,257 - Pf6N2E2_1922 0.11 -4.0 2,275,299 + Pf6N2E2_1922 0.13 -0.6 2,275,299 + Pf6N2E2_1922 0.13 -3.3 2,275,335 - Pf6N2E2_1922 0.14 +0.2 2,275,430 - Pf6N2E2_1922 0.19 -0.8 2,275,501 + Pf6N2E2_1922 0.22 +1.2 2,275,502 - Pf6N2E2_1922 0.22 +1.7 2,275,541 - Pf6N2E2_1922 0.24 -0.5
Or see this region's nucleotide sequence