Experiment: nitrogen source nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1550 and Pf6N2E2_1551 are separated by 201 nucleotides Pf6N2E2_1551 and Pf6N2E2_1552 overlap by 1 nucleotides Pf6N2E2_1552 and Pf6N2E2_1553 are separated by 78 nucleotides
Pf6N2E2_1550 - Multidrug resistance protein B, at 1,808,982 to 1,810,211
_1550
Pf6N2E2_1551 - Transcriptional regulator, at 1,810,413 to 1,811,318
_1551
Pf6N2E2_1552 - Transcriptional regulator, LysR family, at 1,811,318 to 1,812,238
_1552
Pf6N2E2_1553 - Glutathione S-transferase, unnamed subgroup (EC 2.5.1.18), at 1,812,317 to 1,812,946
_1553
Position (kb)
1810
1811
1812 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1809.484 kb on + strand, within Pf6N2E2_1550 at 1809.711 kb on + strand, within Pf6N2E2_1550 at 1809.712 kb on - strand, within Pf6N2E2_1550 at 1809.712 kb on - strand, within Pf6N2E2_1550 at 1809.737 kb on + strand, within Pf6N2E2_1550 at 1809.813 kb on + strand, within Pf6N2E2_1550 at 1809.814 kb on - strand, within Pf6N2E2_1550 at 1809.984 kb on + strand, within Pf6N2E2_1550 at 1809.984 kb on + strand, within Pf6N2E2_1550 at 1809.984 kb on + strand, within Pf6N2E2_1550 at 1809.984 kb on + strand, within Pf6N2E2_1550 at 1809.984 kb on + strand, within Pf6N2E2_1550 at 1809.984 kb on + strand, within Pf6N2E2_1550 at 1809.984 kb on + strand, within Pf6N2E2_1550 at 1809.985 kb on - strand, within Pf6N2E2_1550 at 1809.985 kb on - strand, within Pf6N2E2_1550 at 1809.985 kb on - strand, within Pf6N2E2_1550 at 1809.985 kb on - strand, within Pf6N2E2_1550 at 1810.030 kb on - strand, within Pf6N2E2_1550 at 1810.030 kb on - strand, within Pf6N2E2_1550 at 1810.030 kb on - strand, within Pf6N2E2_1550 at 1810.030 kb on - strand, within Pf6N2E2_1550 at 1810.033 kb on - strand, within Pf6N2E2_1550 at 1810.033 kb on - strand, within Pf6N2E2_1550 at 1810.033 kb on - strand, within Pf6N2E2_1550 at 1810.033 kb on - strand, within Pf6N2E2_1550 at 1810.033 kb on - strand, within Pf6N2E2_1550 at 1810.094 kb on - strand at 1810.116 kb on + strand at 1810.117 kb on - strand at 1810.117 kb on - strand at 1810.117 kb on - strand at 1810.117 kb on - strand at 1810.117 kb on - strand at 1810.125 kb on + strand at 1810.125 kb on + strand at 1810.125 kb on + strand at 1810.126 kb on - strand at 1810.126 kb on - strand at 1810.126 kb on - strand at 1810.165 kb on - strand at 1810.209 kb on + strand at 1810.209 kb on + strand at 1810.210 kb on - strand at 1810.210 kb on - strand at 1810.342 kb on + strand at 1810.342 kb on + strand at 1810.465 kb on + strand at 1810.466 kb on - strand at 1810.466 kb on - strand at 1810.547 kb on - strand, within Pf6N2E2_1551 at 1810.547 kb on - strand, within Pf6N2E2_1551 at 1810.585 kb on + strand, within Pf6N2E2_1551 at 1810.600 kb on + strand, within Pf6N2E2_1551 at 1811.006 kb on - strand, within Pf6N2E2_1551 at 1811.175 kb on + strand, within Pf6N2E2_1551 at 1811.176 kb on - strand, within Pf6N2E2_1551 at 1811.334 kb on - strand at 1811.334 kb on - strand at 1811.334 kb on - strand at 1811.728 kb on - strand, within Pf6N2E2_1552 at 1811.876 kb on + strand, within Pf6N2E2_1552 at 1811.898 kb on + strand, within Pf6N2E2_1552 at 1811.898 kb on + strand, within Pf6N2E2_1552 at 1811.922 kb on + strand, within Pf6N2E2_1552 at 1811.923 kb on - strand, within Pf6N2E2_1552 at 1811.923 kb on - strand, within Pf6N2E2_1552 at 1811.923 kb on - strand, within Pf6N2E2_1552 at 1811.979 kb on + strand, within Pf6N2E2_1552 at 1811.980 kb on - strand, within Pf6N2E2_1552 at 1812.045 kb on + strand, within Pf6N2E2_1552 at 1812.069 kb on + strand, within Pf6N2E2_1552 at 1812.069 kb on + strand, within Pf6N2E2_1552 at 1812.158 kb on - strand at 1812.158 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrate 20mM remove 1,809,484 + Pf6N2E2_1550 0.41 +0.8 1,809,711 + Pf6N2E2_1550 0.59 +0.3 1,809,712 - Pf6N2E2_1550 0.59 -3.5 1,809,712 - Pf6N2E2_1550 0.59 -0.7 1,809,737 + Pf6N2E2_1550 0.61 -0.7 1,809,813 + Pf6N2E2_1550 0.68 -0.0 1,809,814 - Pf6N2E2_1550 0.68 +1.0 1,809,984 + Pf6N2E2_1550 0.81 +1.7 1,809,984 + Pf6N2E2_1550 0.81 -1.1 1,809,984 + Pf6N2E2_1550 0.81 +0.4 1,809,984 + Pf6N2E2_1550 0.81 -1.6 1,809,984 + Pf6N2E2_1550 0.81 +0.1 1,809,984 + Pf6N2E2_1550 0.81 +0.6 1,809,984 + Pf6N2E2_1550 0.81 -0.9 1,809,985 - Pf6N2E2_1550 0.82 +0.8 1,809,985 - Pf6N2E2_1550 0.82 +1.6 1,809,985 - Pf6N2E2_1550 0.82 +1.7 1,809,985 - Pf6N2E2_1550 0.82 +1.7 1,810,030 - Pf6N2E2_1550 0.85 +0.8 1,810,030 - Pf6N2E2_1550 0.85 +0.7 1,810,030 - Pf6N2E2_1550 0.85 -0.0 1,810,030 - Pf6N2E2_1550 0.85 +1.7 1,810,033 - Pf6N2E2_1550 0.85 -2.3 1,810,033 - Pf6N2E2_1550 0.85 -2.0 1,810,033 - Pf6N2E2_1550 0.85 +0.6 1,810,033 - Pf6N2E2_1550 0.85 +0.7 1,810,033 - Pf6N2E2_1550 0.85 -0.2 1,810,094 - +1.7 1,810,116 + +0.1 1,810,117 - +1.5 1,810,117 - +0.1 1,810,117 - -0.7 1,810,117 - -1.9 1,810,117 - -0.8 1,810,125 + +1.7 1,810,125 + +1.1 1,810,125 + -1.2 1,810,126 - -0.5 1,810,126 - +1.7 1,810,126 - +0.9 1,810,165 - -0.3 1,810,209 + -0.5 1,810,209 + +1.5 1,810,210 - +0.7 1,810,210 - -1.5 1,810,342 + -0.2 1,810,342 + -0.7 1,810,465 + -1.8 1,810,466 - -1.1 1,810,466 - +1.7 1,810,547 - Pf6N2E2_1551 0.15 +2.0 1,810,547 - Pf6N2E2_1551 0.15 -1.8 1,810,585 + Pf6N2E2_1551 0.19 +1.7 1,810,600 + Pf6N2E2_1551 0.21 -2.4 1,811,006 - Pf6N2E2_1551 0.65 -2.8 1,811,175 + Pf6N2E2_1551 0.84 -0.6 1,811,176 - Pf6N2E2_1551 0.84 -1.3 1,811,334 - -0.3 1,811,334 - -0.2 1,811,334 - -0.9 1,811,728 - Pf6N2E2_1552 0.45 -0.4 1,811,876 + Pf6N2E2_1552 0.61 +0.1 1,811,898 + Pf6N2E2_1552 0.63 +4.0 1,811,898 + Pf6N2E2_1552 0.63 +0.4 1,811,922 + Pf6N2E2_1552 0.66 +0.5 1,811,923 - Pf6N2E2_1552 0.66 -2.2 1,811,923 - Pf6N2E2_1552 0.66 +0.7 1,811,923 - Pf6N2E2_1552 0.66 -0.6 1,811,979 + Pf6N2E2_1552 0.72 -2.3 1,811,980 - Pf6N2E2_1552 0.72 +1.4 1,812,045 + Pf6N2E2_1552 0.79 -1.3 1,812,069 + Pf6N2E2_1552 0.82 +0.3 1,812,069 + Pf6N2E2_1552 0.82 -2.2 1,812,158 - +0.7 1,812,158 - +0.7
Or see this region's nucleotide sequence