Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6012 and Pf6N2E2_6013 are separated by 429 nucleotides
Pf6N2E2_6012 - Integrase, at 6,830,255 to 6,831,499
_6012
Pf6N2E2_6013 - Conserved hypothetical phage protein, at 6,831,929 to 6,833,128
_6013
Position (kb)
6830
6831
6832 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 6829.297 kb on + strand at 6829.297 kb on + strand at 6829.361 kb on - strand at 6829.652 kb on + strand at 6829.652 kb on + strand at 6829.652 kb on + strand at 6829.653 kb on - strand at 6829.671 kb on - strand at 6829.721 kb on + strand at 6829.721 kb on + strand at 6829.721 kb on + strand at 6829.721 kb on + strand at 6829.721 kb on + strand at 6829.722 kb on - strand at 6829.722 kb on - strand at 6829.722 kb on - strand at 6829.826 kb on + strand at 6829.909 kb on + strand at 6829.931 kb on + strand at 6829.931 kb on + strand at 6829.932 kb on - strand at 6829.950 kb on + strand at 6829.951 kb on - strand at 6830.042 kb on + strand at 6830.043 kb on - strand at 6830.043 kb on - strand at 6830.168 kb on + strand at 6830.169 kb on - strand at 6830.169 kb on - strand at 6830.169 kb on - strand at 6830.171 kb on + strand at 6830.266 kb on + strand at 6830.316 kb on - strand at 6830.316 kb on - strand at 6830.350 kb on - strand at 6830.373 kb on - strand at 6830.420 kb on - strand, within Pf6N2E2_6012 at 6830.512 kb on + strand, within Pf6N2E2_6012 at 6830.513 kb on - strand, within Pf6N2E2_6012 at 6830.515 kb on + strand, within Pf6N2E2_6012 at 6830.515 kb on + strand, within Pf6N2E2_6012 at 6830.567 kb on - strand, within Pf6N2E2_6012 at 6830.610 kb on - strand, within Pf6N2E2_6012 at 6830.610 kb on - strand, within Pf6N2E2_6012 at 6830.610 kb on - strand, within Pf6N2E2_6012 at 6830.663 kb on + strand, within Pf6N2E2_6012 at 6830.664 kb on - strand, within Pf6N2E2_6012 at 6830.664 kb on - strand, within Pf6N2E2_6012 at 6830.664 kb on - strand, within Pf6N2E2_6012 at 6830.738 kb on - strand, within Pf6N2E2_6012 at 6830.752 kb on + strand, within Pf6N2E2_6012 at 6830.752 kb on + strand, within Pf6N2E2_6012 at 6830.752 kb on + strand, within Pf6N2E2_6012 at 6830.753 kb on - strand, within Pf6N2E2_6012 at 6830.982 kb on - strand, within Pf6N2E2_6012 at 6830.982 kb on - strand, within Pf6N2E2_6012 at 6830.982 kb on - strand, within Pf6N2E2_6012 at 6831.023 kb on - strand, within Pf6N2E2_6012 at 6831.023 kb on - strand, within Pf6N2E2_6012 at 6831.026 kb on + strand, within Pf6N2E2_6012 at 6831.026 kb on + strand, within Pf6N2E2_6012 at 6831.027 kb on - strand, within Pf6N2E2_6012 at 6831.051 kb on - strand, within Pf6N2E2_6012 at 6831.067 kb on - strand, within Pf6N2E2_6012 at 6831.067 kb on - strand, within Pf6N2E2_6012 at 6831.067 kb on - strand, within Pf6N2E2_6012 at 6831.067 kb on - strand, within Pf6N2E2_6012 at 6831.067 kb on - strand, within Pf6N2E2_6012 at 6831.067 kb on - strand, within Pf6N2E2_6012 at 6831.067 kb on - strand, within Pf6N2E2_6012 at 6831.067 kb on - strand, within Pf6N2E2_6012 at 6831.138 kb on + strand, within Pf6N2E2_6012 at 6831.138 kb on + strand, within Pf6N2E2_6012 at 6831.139 kb on - strand, within Pf6N2E2_6012 at 6831.321 kb on - strand, within Pf6N2E2_6012 at 6831.321 kb on - strand, within Pf6N2E2_6012 at 6831.321 kb on - strand, within Pf6N2E2_6012 at 6831.373 kb on - strand, within Pf6N2E2_6012 at 6831.418 kb on - strand at 6831.537 kb on - strand at 6831.555 kb on - strand at 6831.662 kb on - strand at 6831.667 kb on + strand at 6831.667 kb on + strand at 6831.667 kb on + strand at 6831.667 kb on + strand at 6831.667 kb on + strand at 6831.668 kb on - strand at 6831.668 kb on - strand at 6831.679 kb on + strand at 6831.680 kb on - strand at 6831.814 kb on + strand at 6831.814 kb on + strand at 6831.814 kb on + strand at 6831.872 kb on + strand at 6831.884 kb on + strand at 6831.884 kb on + strand at 6831.884 kb on + strand at 6831.885 kb on - strand at 6831.885 kb on - strand at 6831.885 kb on - strand at 6831.893 kb on + strand at 6831.894 kb on - strand at 6832.008 kb on - strand at 6832.099 kb on + strand, within Pf6N2E2_6013 at 6832.099 kb on + strand, within Pf6N2E2_6013 at 6832.099 kb on + strand, within Pf6N2E2_6013 at 6832.100 kb on - strand, within Pf6N2E2_6013 at 6832.100 kb on - strand, within Pf6N2E2_6013 at 6832.100 kb on - strand, within Pf6N2E2_6013 at 6832.100 kb on - strand, within Pf6N2E2_6013 at 6832.100 kb on - strand, within Pf6N2E2_6013 at 6832.143 kb on + strand, within Pf6N2E2_6013 at 6832.277 kb on + strand, within Pf6N2E2_6013 at 6832.277 kb on + strand, within Pf6N2E2_6013 at 6832.472 kb on + strand, within Pf6N2E2_6013
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 6,829,297 + -0.1 6,829,297 + -0.0 6,829,361 - -1.6 6,829,652 + -0.2 6,829,652 + +0.9 6,829,652 + -0.0 6,829,653 - +0.0 6,829,671 - -1.2 6,829,721 + -0.4 6,829,721 + -0.0 6,829,721 + +0.8 6,829,721 + -0.6 6,829,721 + +0.3 6,829,722 - -2.3 6,829,722 - +1.6 6,829,722 - +0.7 6,829,826 + -0.1 6,829,909 + -0.3 6,829,931 + +1.0 6,829,931 + +0.6 6,829,932 - +0.6 6,829,950 + -0.4 6,829,951 - +0.6 6,830,042 + +0.4 6,830,043 - -1.1 6,830,043 - -1.6 6,830,168 + -1.2 6,830,169 - -0.0 6,830,169 - -0.7 6,830,169 - -0.2 6,830,171 + +0.1 6,830,266 + -0.3 6,830,316 - +0.6 6,830,316 - -0.1 6,830,350 - -0.2 6,830,373 - -1.4 6,830,420 - Pf6N2E2_6012 0.13 -0.2 6,830,512 + Pf6N2E2_6012 0.21 +1.6 6,830,513 - Pf6N2E2_6012 0.21 +0.2 6,830,515 + Pf6N2E2_6012 0.21 +2.0 6,830,515 + Pf6N2E2_6012 0.21 +0.1 6,830,567 - Pf6N2E2_6012 0.25 +0.2 6,830,610 - Pf6N2E2_6012 0.29 -1.4 6,830,610 - Pf6N2E2_6012 0.29 +0.4 6,830,610 - Pf6N2E2_6012 0.29 -0.2 6,830,663 + Pf6N2E2_6012 0.33 +0.0 6,830,664 - Pf6N2E2_6012 0.33 +1.3 6,830,664 - Pf6N2E2_6012 0.33 +0.6 6,830,664 - Pf6N2E2_6012 0.33 +0.6 6,830,738 - Pf6N2E2_6012 0.39 +0.5 6,830,752 + Pf6N2E2_6012 0.40 +0.4 6,830,752 + Pf6N2E2_6012 0.40 -0.4 6,830,752 + Pf6N2E2_6012 0.40 -0.4 6,830,753 - Pf6N2E2_6012 0.40 +1.1 6,830,982 - Pf6N2E2_6012 0.58 +0.1 6,830,982 - Pf6N2E2_6012 0.58 -0.2 6,830,982 - Pf6N2E2_6012 0.58 -0.4 6,831,023 - Pf6N2E2_6012 0.62 -0.3 6,831,023 - Pf6N2E2_6012 0.62 -2.2 6,831,026 + Pf6N2E2_6012 0.62 -0.7 6,831,026 + Pf6N2E2_6012 0.62 +0.6 6,831,027 - Pf6N2E2_6012 0.62 +1.0 6,831,051 - Pf6N2E2_6012 0.64 +1.6 6,831,067 - Pf6N2E2_6012 0.65 -1.7 6,831,067 - Pf6N2E2_6012 0.65 +0.6 6,831,067 - Pf6N2E2_6012 0.65 +0.5 6,831,067 - Pf6N2E2_6012 0.65 +1.1 6,831,067 - Pf6N2E2_6012 0.65 +0.6 6,831,067 - Pf6N2E2_6012 0.65 +1.6 6,831,067 - Pf6N2E2_6012 0.65 +0.6 6,831,067 - Pf6N2E2_6012 0.65 +1.6 6,831,138 + Pf6N2E2_6012 0.71 +0.6 6,831,138 + Pf6N2E2_6012 0.71 -0.5 6,831,139 - Pf6N2E2_6012 0.71 -2.7 6,831,321 - Pf6N2E2_6012 0.86 +0.6 6,831,321 - Pf6N2E2_6012 0.86 -0.7 6,831,321 - Pf6N2E2_6012 0.86 -1.5 6,831,373 - Pf6N2E2_6012 0.90 +0.4 6,831,418 - -0.0 6,831,537 - -0.4 6,831,555 - +0.1 6,831,662 - +0.1 6,831,667 + +0.6 6,831,667 + +1.6 6,831,667 + +0.3 6,831,667 + -0.0 6,831,667 + -0.5 6,831,668 - -1.9 6,831,668 - +0.6 6,831,679 + +0.3 6,831,680 - -0.6 6,831,814 + +0.9 6,831,814 + +0.6 6,831,814 + -0.5 6,831,872 + +3.9 6,831,884 + -2.0 6,831,884 + -0.6 6,831,884 + +1.6 6,831,885 - -0.1 6,831,885 - +0.2 6,831,885 - -0.9 6,831,893 + +0.4 6,831,894 - -0.2 6,832,008 - +0.3 6,832,099 + Pf6N2E2_6013 0.14 +0.6 6,832,099 + Pf6N2E2_6013 0.14 -0.6 6,832,099 + Pf6N2E2_6013 0.14 -1.0 6,832,100 - Pf6N2E2_6013 0.14 +3.2 6,832,100 - Pf6N2E2_6013 0.14 +0.3 6,832,100 - Pf6N2E2_6013 0.14 +0.5 6,832,100 - Pf6N2E2_6013 0.14 +0.1 6,832,100 - Pf6N2E2_6013 0.14 +1.7 6,832,143 + Pf6N2E2_6013 0.18 -0.3 6,832,277 + Pf6N2E2_6013 0.29 -0.3 6,832,277 + Pf6N2E2_6013 0.29 -0.4 6,832,472 + Pf6N2E2_6013 0.45 -2.2
Or see this region's nucleotide sequence