Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3933 and Pf6N2E2_3934 are separated by 42 nucleotides Pf6N2E2_3934 and Pf6N2E2_3935 are separated by 231 nucleotides Pf6N2E2_3935 and Pf6N2E2_3936 are separated by 121 nucleotides
Pf6N2E2_3933 - D-2-hydroxyglutarate dehydrogenase, at 4,569,647 to 4,571,065
_3933
Pf6N2E2_3934 - Fumarylacetoacetate hydrolase family protein, at 4,571,108 to 4,571,773
_3934
Pf6N2E2_3935 - Outer membrane protein assembly factor YaeT precursor, at 4,572,005 to 4,572,937
_3935
Pf6N2E2_3936 - Outer membrane protein assembly factor YaeT precursor, at 4,573,059 to 4,573,976
_3936
Position (kb)
4572
4573 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4571.114 kb on + strand at 4571.115 kb on - strand at 4571.115 kb on - strand at 4571.115 kb on - strand at 4571.115 kb on - strand at 4571.126 kb on + strand at 4571.126 kb on + strand at 4571.126 kb on + strand at 4571.127 kb on - strand at 4571.127 kb on - strand at 4571.127 kb on - strand at 4571.143 kb on + strand at 4571.143 kb on + strand at 4571.143 kb on + strand at 4571.143 kb on + strand at 4571.143 kb on + strand at 4571.144 kb on - strand at 4571.187 kb on - strand, within Pf6N2E2_3934 at 4571.187 kb on - strand, within Pf6N2E2_3934 at 4571.315 kb on + strand, within Pf6N2E2_3934 at 4571.315 kb on + strand, within Pf6N2E2_3934 at 4571.316 kb on - strand, within Pf6N2E2_3934 at 4571.316 kb on - strand, within Pf6N2E2_3934 at 4571.379 kb on + strand, within Pf6N2E2_3934 at 4571.380 kb on - strand, within Pf6N2E2_3934 at 4571.380 kb on - strand, within Pf6N2E2_3934 at 4571.590 kb on + strand, within Pf6N2E2_3934 at 4571.591 kb on - strand, within Pf6N2E2_3934 at 4571.591 kb on - strand, within Pf6N2E2_3934 at 4571.593 kb on + strand, within Pf6N2E2_3934 at 4571.594 kb on - strand, within Pf6N2E2_3934 at 4571.594 kb on - strand, within Pf6N2E2_3934 at 4571.594 kb on - strand, within Pf6N2E2_3934 at 4571.594 kb on - strand, within Pf6N2E2_3934 at 4571.633 kb on + strand, within Pf6N2E2_3934 at 4571.633 kb on + strand, within Pf6N2E2_3934 at 4571.633 kb on + strand, within Pf6N2E2_3934 at 4571.633 kb on + strand, within Pf6N2E2_3934 at 4571.633 kb on + strand, within Pf6N2E2_3934 at 4571.634 kb on - strand, within Pf6N2E2_3934 at 4571.634 kb on - strand, within Pf6N2E2_3934 at 4571.634 kb on - strand, within Pf6N2E2_3934 at 4571.634 kb on - strand, within Pf6N2E2_3934 at 4571.634 kb on - strand, within Pf6N2E2_3934 at 4571.634 kb on - strand, within Pf6N2E2_3934 at 4571.634 kb on - strand, within Pf6N2E2_3934 at 4571.778 kb on + strand at 4571.910 kb on + strand at 4571.911 kb on - strand at 4572.071 kb on + strand at 4572.071 kb on + strand at 4572.071 kb on + strand at 4572.072 kb on - strand at 4572.072 kb on - strand at 4572.072 kb on - strand at 4572.105 kb on - strand, within Pf6N2E2_3935 at 4572.105 kb on - strand, within Pf6N2E2_3935 at 4572.275 kb on + strand, within Pf6N2E2_3935 at 4572.275 kb on + strand, within Pf6N2E2_3935 at 4572.275 kb on + strand, within Pf6N2E2_3935 at 4572.275 kb on + strand, within Pf6N2E2_3935 at 4572.276 kb on - strand, within Pf6N2E2_3935 at 4572.276 kb on - strand, within Pf6N2E2_3935 at 4572.276 kb on - strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.404 kb on + strand, within Pf6N2E2_3935 at 4572.405 kb on - strand, within Pf6N2E2_3935 at 4572.405 kb on - strand, within Pf6N2E2_3935 at 4572.405 kb on - strand, within Pf6N2E2_3935 at 4572.405 kb on - strand, within Pf6N2E2_3935 at 4572.405 kb on - strand, within Pf6N2E2_3935 at 4572.405 kb on - strand, within Pf6N2E2_3935 at 4572.405 kb on - strand, within Pf6N2E2_3935 at 4572.405 kb on - strand, within Pf6N2E2_3935 at 4572.406 kb on + strand, within Pf6N2E2_3935 at 4572.406 kb on + strand, within Pf6N2E2_3935 at 4572.406 kb on + strand, within Pf6N2E2_3935 at 4572.406 kb on + strand, within Pf6N2E2_3935 at 4572.406 kb on + strand, within Pf6N2E2_3935 at 4572.406 kb on + strand, within Pf6N2E2_3935 at 4572.406 kb on + strand, within Pf6N2E2_3935 at 4572.406 kb on + strand, within Pf6N2E2_3935 at 4572.407 kb on - strand, within Pf6N2E2_3935 at 4572.407 kb on - strand, within Pf6N2E2_3935 at 4572.407 kb on - strand, within Pf6N2E2_3935 at 4572.407 kb on - strand, within Pf6N2E2_3935 at 4572.512 kb on + strand, within Pf6N2E2_3935 at 4572.512 kb on + strand, within Pf6N2E2_3935 at 4572.512 kb on + strand, within Pf6N2E2_3935 at 4572.512 kb on + strand, within Pf6N2E2_3935 at 4572.512 kb on + strand, within Pf6N2E2_3935 at 4572.512 kb on + strand, within Pf6N2E2_3935 at 4572.513 kb on - strand, within Pf6N2E2_3935 at 4572.513 kb on - strand, within Pf6N2E2_3935 at 4572.588 kb on + strand, within Pf6N2E2_3935 at 4572.887 kb on + strand at 4572.887 kb on + strand at 4572.887 kb on + strand at 4572.887 kb on + strand at 4572.888 kb on - strand at 4572.888 kb on - strand at 4572.888 kb on - strand at 4572.888 kb on - strand at 4572.888 kb on - strand at 4572.914 kb on + strand at 4572.915 kb on - strand at 4572.915 kb on - strand at 4572.915 kb on - strand at 4572.915 kb on - strand at 4572.915 kb on - strand at 4572.944 kb on + strand at 4572.944 kb on + strand at 4572.945 kb on - strand at 4572.945 kb on - strand at 4572.957 kb on + strand at 4572.957 kb on + strand at 4572.957 kb on + strand at 4572.957 kb on + strand at 4572.958 kb on - strand at 4572.958 kb on - strand at 4572.958 kb on - strand at 4572.958 kb on - strand at 4572.974 kb on + strand at 4572.975 kb on - strand at 4572.995 kb on + strand at 4573.040 kb on + strand at 4573.349 kb on - strand, within Pf6N2E2_3936 at 4573.434 kb on + strand, within Pf6N2E2_3936 at 4573.434 kb on + strand, within Pf6N2E2_3936 at 4573.434 kb on + strand, within Pf6N2E2_3936 at 4573.434 kb on + strand, within Pf6N2E2_3936 at 4573.434 kb on + strand, within Pf6N2E2_3936 at 4573.434 kb on + strand, within Pf6N2E2_3936 at 4573.434 kb on + strand, within Pf6N2E2_3936 at 4573.434 kb on + strand, within Pf6N2E2_3936 at 4573.434 kb on + strand, within Pf6N2E2_3936 at 4573.434 kb on + strand, within Pf6N2E2_3936 at 4573.434 kb on + strand, within Pf6N2E2_3936 at 4573.434 kb on + strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.435 kb on - strand, within Pf6N2E2_3936 at 4573.526 kb on - strand, within Pf6N2E2_3936 at 4573.576 kb on - strand, within Pf6N2E2_3936 at 4573.585 kb on + strand, within Pf6N2E2_3936 at 4573.632 kb on + strand, within Pf6N2E2_3936 at 4573.632 kb on + strand, within Pf6N2E2_3936 at 4573.633 kb on - strand, within Pf6N2E2_3936 at 4573.674 kb on + strand, within Pf6N2E2_3936 at 4573.675 kb on - strand, within Pf6N2E2_3936 at 4573.746 kb on + strand, within Pf6N2E2_3936 at 4573.746 kb on + strand, within Pf6N2E2_3936 at 4573.746 kb on + strand, within Pf6N2E2_3936 at 4573.769 kb on - strand, within Pf6N2E2_3936 at 4573.769 kb on - strand, within Pf6N2E2_3936 at 4573.769 kb on - strand, within Pf6N2E2_3936 at 4573.769 kb on - strand, within Pf6N2E2_3936 at 4573.769 kb on - strand, within Pf6N2E2_3936 at 4573.769 kb on - strand, within Pf6N2E2_3936 at 4573.769 kb on - strand, within Pf6N2E2_3936 at 4573.920 kb on + strand at 4573.921 kb on - strand at 4573.921 kb on - strand at 4573.921 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 4,571,114 + +0.9 4,571,115 - +0.6 4,571,115 - +0.0 4,571,115 - +1.6 4,571,115 - +1.6 4,571,126 + +0.1 4,571,126 + +0.4 4,571,126 + +0.3 4,571,127 - +1.6 4,571,127 - +0.4 4,571,127 - -1.4 4,571,143 + +1.4 4,571,143 + -0.8 4,571,143 + +0.9 4,571,143 + -0.2 4,571,143 + -0.3 4,571,144 - +1.6 4,571,187 - Pf6N2E2_3934 0.12 +0.3 4,571,187 - Pf6N2E2_3934 0.12 -0.8 4,571,315 + Pf6N2E2_3934 0.31 -1.0 4,571,315 + Pf6N2E2_3934 0.31 +0.1 4,571,316 - Pf6N2E2_3934 0.31 -0.3 4,571,316 - Pf6N2E2_3934 0.31 +0.3 4,571,379 + Pf6N2E2_3934 0.41 -0.1 4,571,380 - Pf6N2E2_3934 0.41 -0.2 4,571,380 - Pf6N2E2_3934 0.41 +0.1 4,571,590 + Pf6N2E2_3934 0.72 -1.8 4,571,591 - Pf6N2E2_3934 0.73 +0.4 4,571,591 - Pf6N2E2_3934 0.73 +0.8 4,571,593 + Pf6N2E2_3934 0.73 -0.6 4,571,594 - Pf6N2E2_3934 0.73 -0.7 4,571,594 - Pf6N2E2_3934 0.73 +0.0 4,571,594 - Pf6N2E2_3934 0.73 -0.4 4,571,594 - Pf6N2E2_3934 0.73 +0.3 4,571,633 + Pf6N2E2_3934 0.79 +0.6 4,571,633 + Pf6N2E2_3934 0.79 +0.2 4,571,633 + Pf6N2E2_3934 0.79 -0.3 4,571,633 + Pf6N2E2_3934 0.79 +0.6 4,571,633 + Pf6N2E2_3934 0.79 +0.5 4,571,634 - Pf6N2E2_3934 0.79 -0.3 4,571,634 - Pf6N2E2_3934 0.79 +1.2 4,571,634 - Pf6N2E2_3934 0.79 +1.0 4,571,634 - Pf6N2E2_3934 0.79 -1.6 4,571,634 - Pf6N2E2_3934 0.79 +1.6 4,571,634 - Pf6N2E2_3934 0.79 -0.4 4,571,634 - Pf6N2E2_3934 0.79 -0.1 4,571,778 + -1.0 4,571,910 + -1.0 4,571,911 - -1.4 4,572,071 + -1.6 4,572,071 + +1.2 4,572,071 + -0.0 4,572,072 - +0.0 4,572,072 - -0.2 4,572,072 - -1.0 4,572,105 - Pf6N2E2_3935 0.11 -1.8 4,572,105 - Pf6N2E2_3935 0.11 -3.2 4,572,275 + Pf6N2E2_3935 0.29 -0.4 4,572,275 + Pf6N2E2_3935 0.29 -1.2 4,572,275 + Pf6N2E2_3935 0.29 +0.2 4,572,275 + Pf6N2E2_3935 0.29 +0.3 4,572,276 - Pf6N2E2_3935 0.29 +0.6 4,572,276 - Pf6N2E2_3935 0.29 +1.2 4,572,276 - Pf6N2E2_3935 0.29 +0.1 4,572,404 + Pf6N2E2_3935 0.43 +0.2 4,572,404 + Pf6N2E2_3935 0.43 +0.3 4,572,404 + Pf6N2E2_3935 0.43 -0.7 4,572,404 + Pf6N2E2_3935 0.43 +0.2 4,572,404 + Pf6N2E2_3935 0.43 +0.9 4,572,404 + Pf6N2E2_3935 0.43 +0.0 4,572,404 + Pf6N2E2_3935 0.43 -0.2 4,572,404 + Pf6N2E2_3935 0.43 +1.0 4,572,404 + Pf6N2E2_3935 0.43 +1.6 4,572,404 + Pf6N2E2_3935 0.43 -0.7 4,572,404 + Pf6N2E2_3935 0.43 -1.0 4,572,404 + Pf6N2E2_3935 0.43 -2.8 4,572,404 + Pf6N2E2_3935 0.43 +1.0 4,572,404 + Pf6N2E2_3935 0.43 +1.6 4,572,404 + Pf6N2E2_3935 0.43 +0.6 4,572,404 + Pf6N2E2_3935 0.43 -0.1 4,572,404 + Pf6N2E2_3935 0.43 -2.6 4,572,404 + Pf6N2E2_3935 0.43 -1.0 4,572,404 + Pf6N2E2_3935 0.43 -1.9 4,572,404 + Pf6N2E2_3935 0.43 +2.6 4,572,405 - Pf6N2E2_3935 0.43 +0.0 4,572,405 - Pf6N2E2_3935 0.43 +0.1 4,572,405 - Pf6N2E2_3935 0.43 +0.0 4,572,405 - Pf6N2E2_3935 0.43 +0.6 4,572,405 - Pf6N2E2_3935 0.43 -0.2 4,572,405 - Pf6N2E2_3935 0.43 -0.7 4,572,405 - Pf6N2E2_3935 0.43 +1.6 4,572,405 - Pf6N2E2_3935 0.43 +0.8 4,572,406 + Pf6N2E2_3935 0.43 +0.0 4,572,406 + Pf6N2E2_3935 0.43 +0.2 4,572,406 + Pf6N2E2_3935 0.43 -0.2 4,572,406 + Pf6N2E2_3935 0.43 -0.9 4,572,406 + Pf6N2E2_3935 0.43 +1.0 4,572,406 + Pf6N2E2_3935 0.43 -1.5 4,572,406 + Pf6N2E2_3935 0.43 +0.8 4,572,406 + Pf6N2E2_3935 0.43 +0.0 4,572,407 - Pf6N2E2_3935 0.43 -2.6 4,572,407 - Pf6N2E2_3935 0.43 +1.0 4,572,407 - Pf6N2E2_3935 0.43 -0.5 4,572,407 - Pf6N2E2_3935 0.43 +0.0 4,572,512 + Pf6N2E2_3935 0.54 +0.5 4,572,512 + Pf6N2E2_3935 0.54 +1.6 4,572,512 + Pf6N2E2_3935 0.54 +1.0 4,572,512 + Pf6N2E2_3935 0.54 +0.2 4,572,512 + Pf6N2E2_3935 0.54 +0.0 4,572,512 + Pf6N2E2_3935 0.54 -0.7 4,572,513 - Pf6N2E2_3935 0.54 +0.3 4,572,513 - Pf6N2E2_3935 0.54 -2.4 4,572,588 + Pf6N2E2_3935 0.62 -0.3 4,572,887 + +0.2 4,572,887 + -1.0 4,572,887 + +0.0 4,572,887 + -0.4 4,572,888 - +1.0 4,572,888 - -1.0 4,572,888 - +0.2 4,572,888 - +0.4 4,572,888 - -0.0 4,572,914 + +1.2 4,572,915 - +0.6 4,572,915 - +0.0 4,572,915 - +0.7 4,572,915 - -0.2 4,572,915 - +1.6 4,572,944 + -0.2 4,572,944 + -0.7 4,572,945 - -2.0 4,572,945 - -1.5 4,572,957 + +1.2 4,572,957 + -1.4 4,572,957 + +0.2 4,572,957 + -1.0 4,572,958 - +0.6 4,572,958 - +0.6 4,572,958 - +1.9 4,572,958 - +0.5 4,572,974 + -0.9 4,572,975 - -0.5 4,572,995 + -0.0 4,573,040 + +0.6 4,573,349 - Pf6N2E2_3936 0.32 +0.0 4,573,434 + Pf6N2E2_3936 0.41 +0.0 4,573,434 + Pf6N2E2_3936 0.41 +0.2 4,573,434 + Pf6N2E2_3936 0.41 +1.0 4,573,434 + Pf6N2E2_3936 0.41 +1.0 4,573,434 + Pf6N2E2_3936 0.41 -0.8 4,573,434 + Pf6N2E2_3936 0.41 -0.7 4,573,434 + Pf6N2E2_3936 0.41 -0.2 4,573,434 + Pf6N2E2_3936 0.41 +0.2 4,573,434 + Pf6N2E2_3936 0.41 +0.3 4,573,434 + Pf6N2E2_3936 0.41 +0.0 4,573,434 + Pf6N2E2_3936 0.41 +0.1 4,573,434 + Pf6N2E2_3936 0.41 -1.4 4,573,435 - Pf6N2E2_3936 0.41 -0.1 4,573,435 - Pf6N2E2_3936 0.41 +0.9 4,573,435 - Pf6N2E2_3936 0.41 +0.3 4,573,435 - Pf6N2E2_3936 0.41 -2.0 4,573,435 - Pf6N2E2_3936 0.41 +0.1 4,573,435 - Pf6N2E2_3936 0.41 -0.4 4,573,435 - Pf6N2E2_3936 0.41 -0.1 4,573,435 - Pf6N2E2_3936 0.41 -0.7 4,573,435 - Pf6N2E2_3936 0.41 +0.1 4,573,435 - Pf6N2E2_3936 0.41 -0.1 4,573,435 - Pf6N2E2_3936 0.41 +0.0 4,573,435 - Pf6N2E2_3936 0.41 +0.2 4,573,435 - Pf6N2E2_3936 0.41 -0.6 4,573,435 - Pf6N2E2_3936 0.41 +0.0 4,573,435 - Pf6N2E2_3936 0.41 +0.2 4,573,435 - Pf6N2E2_3936 0.41 +0.0 4,573,435 - Pf6N2E2_3936 0.41 +0.3 4,573,526 - Pf6N2E2_3936 0.51 -0.3 4,573,576 - Pf6N2E2_3936 0.56 +0.5 4,573,585 + Pf6N2E2_3936 0.57 -2.0 4,573,632 + Pf6N2E2_3936 0.62 +0.2 4,573,632 + Pf6N2E2_3936 0.62 +1.6 4,573,633 - Pf6N2E2_3936 0.63 -3.0 4,573,674 + Pf6N2E2_3936 0.67 +0.2 4,573,675 - Pf6N2E2_3936 0.67 -2.2 4,573,746 + Pf6N2E2_3936 0.75 +0.2 4,573,746 + Pf6N2E2_3936 0.75 +0.3 4,573,746 + Pf6N2E2_3936 0.75 -1.4 4,573,769 - Pf6N2E2_3936 0.77 -0.3 4,573,769 - Pf6N2E2_3936 0.77 -0.4 4,573,769 - Pf6N2E2_3936 0.77 +0.6 4,573,769 - Pf6N2E2_3936 0.77 -1.0 4,573,769 - Pf6N2E2_3936 0.77 -1.0 4,573,769 - Pf6N2E2_3936 0.77 +0.2 4,573,769 - Pf6N2E2_3936 0.77 -1.0 4,573,920 + +0.0 4,573,921 - +0.8 4,573,921 - -0.4 4,573,921 - -1.1
Or see this region's nucleotide sequence