Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_213

Experiment: nitrogen source Ammonium chloride 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_212 and Pf6N2E2_213 are separated by 48 nucleotidesPf6N2E2_213 and Pf6N2E2_214 are separated by 25 nucleotides Pf6N2E2_212 - Glycosyl transferase, group 2 family protein, at 267,772 to 269,946 _212 Pf6N2E2_213 - hypothetical protein, at 269,995 to 270,831 _213 Pf6N2E2_214 - dTDP-Rha:A-D-GlcNAc-diphosphoryl polyprenol, A-3-L-rhamnosyl transferase WbbL, at 270,857 to 271,807 _214 Position (kb) 269 270 271Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 269.065 kb on + strand, within Pf6N2E2_212at 269.066 kb on - strand, within Pf6N2E2_212at 269.066 kb on - strand, within Pf6N2E2_212at 269.066 kb on - strand, within Pf6N2E2_212at 269.066 kb on - strand, within Pf6N2E2_212at 269.066 kb on - strand, within Pf6N2E2_212at 269.176 kb on + strand, within Pf6N2E2_212at 269.176 kb on + strand, within Pf6N2E2_212at 269.176 kb on + strand, within Pf6N2E2_212at 269.176 kb on + strand, within Pf6N2E2_212at 269.177 kb on - strand, within Pf6N2E2_212at 269.227 kb on - strand, within Pf6N2E2_212at 269.429 kb on + strand, within Pf6N2E2_212at 269.430 kb on - strand, within Pf6N2E2_212at 269.514 kb on - strandat 269.720 kb on - strand, within Pf6N2E2_212at 269.827 kb on + strandat 269.858 kb on - strandat 270.297 kb on + strand, within Pf6N2E2_213at 270.297 kb on + strand, within Pf6N2E2_213at 270.297 kb on + strand, within Pf6N2E2_213at 270.298 kb on - strand, within Pf6N2E2_213at 270.298 kb on - strand, within Pf6N2E2_213at 270.298 kb on - strand, within Pf6N2E2_213at 270.298 kb on - strand, within Pf6N2E2_213at 270.298 kb on - strand, within Pf6N2E2_213at 270.298 kb on - strand, within Pf6N2E2_213at 270.443 kb on + strand, within Pf6N2E2_213at 270.443 kb on + strand, within Pf6N2E2_213at 270.444 kb on - strand, within Pf6N2E2_213at 270.444 kb on - strand, within Pf6N2E2_213at 270.444 kb on - strand, within Pf6N2E2_213at 270.444 kb on - strand, within Pf6N2E2_213at 270.444 kb on - strand, within Pf6N2E2_213at 270.638 kb on + strand, within Pf6N2E2_213at 270.832 kb on + strandat 270.858 kb on + strandat 270.858 kb on + strandat 270.859 kb on - strandat 270.863 kb on + strandat 270.864 kb on - strandat 270.966 kb on + strand, within Pf6N2E2_214at 270.966 kb on + strand, within Pf6N2E2_214at 270.966 kb on + strand, within Pf6N2E2_214at 270.967 kb on - strand, within Pf6N2E2_214at 270.991 kb on - strand, within Pf6N2E2_214at 271.061 kb on + strand, within Pf6N2E2_214at 271.061 kb on + strand, within Pf6N2E2_214at 271.061 kb on + strand, within Pf6N2E2_214at 271.062 kb on - strand, within Pf6N2E2_214at 271.062 kb on - strand, within Pf6N2E2_214at 271.155 kb on + strand, within Pf6N2E2_214at 271.156 kb on - strand, within Pf6N2E2_214at 271.156 kb on - strand, within Pf6N2E2_214at 271.269 kb on - strand, within Pf6N2E2_214at 271.445 kb on + strand, within Pf6N2E2_214at 271.445 kb on + strand, within Pf6N2E2_214at 271.457 kb on + strand, within Pf6N2E2_214at 271.457 kb on + strand, within Pf6N2E2_214at 271.689 kb on - strand, within Pf6N2E2_214at 271.689 kb on - strand, within Pf6N2E2_214at 271.719 kb on + strandat 271.720 kb on - strandat 271.720 kb on - strandat 271.830 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM
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269,065 + Pf6N2E2_212 0.59 -1.0
269,066 - Pf6N2E2_212 0.59 +0.5
269,066 - Pf6N2E2_212 0.59 +1.5
269,066 - Pf6N2E2_212 0.59 +1.5
269,066 - Pf6N2E2_212 0.59 -1.5
269,066 - Pf6N2E2_212 0.59 -1.0
269,176 + Pf6N2E2_212 0.65 -1.1
269,176 + Pf6N2E2_212 0.65 -2.0
269,176 + Pf6N2E2_212 0.65 -0.0
269,176 + Pf6N2E2_212 0.65 +1.5
269,177 - Pf6N2E2_212 0.65 +0.0
269,227 - Pf6N2E2_212 0.67 +1.7
269,429 + Pf6N2E2_212 0.76 +0.7
269,430 - Pf6N2E2_212 0.76 +1.5
269,514 - +1.0
269,720 - Pf6N2E2_212 0.90 +0.7
269,827 + -2.2
269,858 - -0.6
270,297 + Pf6N2E2_213 0.36 -1.1
270,297 + Pf6N2E2_213 0.36 +0.4
270,297 + Pf6N2E2_213 0.36 -2.0
270,298 - Pf6N2E2_213 0.36 -4.0
270,298 - Pf6N2E2_213 0.36 -0.7
270,298 - Pf6N2E2_213 0.36 -0.3
270,298 - Pf6N2E2_213 0.36 +0.3
270,298 - Pf6N2E2_213 0.36 +0.3
270,298 - Pf6N2E2_213 0.36 -2.5
270,443 + Pf6N2E2_213 0.54 -1.3
270,443 + Pf6N2E2_213 0.54 -1.3
270,444 - Pf6N2E2_213 0.54 -0.2
270,444 - Pf6N2E2_213 0.54 -0.0
270,444 - Pf6N2E2_213 0.54 -0.8
270,444 - Pf6N2E2_213 0.54 -0.1
270,444 - Pf6N2E2_213 0.54 -0.5
270,638 + Pf6N2E2_213 0.77 +1.5
270,832 + -0.3
270,858 + -0.1
270,858 + -0.0
270,859 - +0.2
270,863 + +1.5
270,864 - -2.0
270,966 + Pf6N2E2_214 0.11 +0.1
270,966 + Pf6N2E2_214 0.11 -2.5
270,966 + Pf6N2E2_214 0.11 +0.4
270,967 - Pf6N2E2_214 0.12 -1.4
270,991 - Pf6N2E2_214 0.14 +0.3
271,061 + Pf6N2E2_214 0.21 -0.2
271,061 + Pf6N2E2_214 0.21 +0.7
271,061 + Pf6N2E2_214 0.21 -1.0
271,062 - Pf6N2E2_214 0.22 +0.4
271,062 - Pf6N2E2_214 0.22 +1.5
271,155 + Pf6N2E2_214 0.31 -1.2
271,156 - Pf6N2E2_214 0.31 +1.5
271,156 - Pf6N2E2_214 0.31 -2.0
271,269 - Pf6N2E2_214 0.43 -0.4
271,445 + Pf6N2E2_214 0.62 -1.3
271,445 + Pf6N2E2_214 0.62 +1.6
271,457 + Pf6N2E2_214 0.63 +0.1
271,457 + Pf6N2E2_214 0.63 +0.5
271,689 - Pf6N2E2_214 0.87 -1.5
271,689 - Pf6N2E2_214 0.87 +1.5
271,719 + -0.8
271,720 - -1.6
271,720 - -0.0
271,830 + +0.1

Or see this region's nucleotide sequence