Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5672 and Pf6N2E2_5673 are separated by 228 nucleotides Pf6N2E2_5673 and Pf6N2E2_5674 overlap by 39 nucleotides Pf6N2E2_5674 and Pf6N2E2_5675 are separated by 146 nucleotides
Pf6N2E2_5672 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 6,485,128 to 6,485,637
_5672
Pf6N2E2_5673 - hypothetical protein, at 6,485,866 to 6,486,216
_5673
Pf6N2E2_5674 - Low-specificity L-threonine aldolase (EC 4.1.2.48), at 6,486,178 to 6,487,182
_5674
Pf6N2E2_5675 - Alanyl-tRNA synthetase (EC 6.1.1.7), at 6,487,329 to 6,489,950
_5675
Position (kb)
6486
6487
6488 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6485.185 kb on - strand, within Pf6N2E2_5672 at 6485.369 kb on + strand, within Pf6N2E2_5672 at 6485.369 kb on + strand, within Pf6N2E2_5672 at 6485.369 kb on + strand, within Pf6N2E2_5672 at 6485.370 kb on - strand, within Pf6N2E2_5672 at 6485.370 kb on - strand, within Pf6N2E2_5672 at 6485.370 kb on - strand, within Pf6N2E2_5672 at 6485.370 kb on - strand, within Pf6N2E2_5672 at 6485.370 kb on - strand, within Pf6N2E2_5672 at 6485.487 kb on + strand, within Pf6N2E2_5672 at 6485.696 kb on + strand at 6485.761 kb on + strand at 6485.761 kb on + strand at 6485.761 kb on + strand at 6485.761 kb on + strand at 6485.762 kb on - strand at 6485.848 kb on + strand at 6485.884 kb on + strand at 6485.884 kb on + strand at 6485.884 kb on + strand at 6485.884 kb on + strand at 6485.885 kb on - strand at 6485.947 kb on + strand, within Pf6N2E2_5673 at 6485.947 kb on + strand, within Pf6N2E2_5673 at 6485.947 kb on + strand, within Pf6N2E2_5673 at 6485.947 kb on + strand, within Pf6N2E2_5673 at 6485.947 kb on + strand, within Pf6N2E2_5673 at 6485.948 kb on - strand, within Pf6N2E2_5673 at 6485.948 kb on - strand, within Pf6N2E2_5673 at 6485.948 kb on - strand, within Pf6N2E2_5673 at 6485.948 kb on - strand, within Pf6N2E2_5673 at 6486.084 kb on + strand, within Pf6N2E2_5673 at 6486.084 kb on + strand, within Pf6N2E2_5673 at 6486.084 kb on + strand, within Pf6N2E2_5673 at 6486.085 kb on - strand, within Pf6N2E2_5673 at 6486.107 kb on - strand, within Pf6N2E2_5673 at 6486.127 kb on + strand, within Pf6N2E2_5673 at 6486.127 kb on + strand, within Pf6N2E2_5673 at 6486.128 kb on - strand, within Pf6N2E2_5673 at 6486.128 kb on - strand, within Pf6N2E2_5673 at 6486.128 kb on - strand, within Pf6N2E2_5673 at 6486.271 kb on + strand at 6486.271 kb on + strand at 6486.272 kb on - strand at 6486.366 kb on + strand, within Pf6N2E2_5674 at 6486.367 kb on - strand, within Pf6N2E2_5674 at 6486.409 kb on + strand, within Pf6N2E2_5674 at 6486.409 kb on + strand, within Pf6N2E2_5674 at 6486.410 kb on - strand, within Pf6N2E2_5674 at 6486.410 kb on - strand, within Pf6N2E2_5674 at 6486.410 kb on - strand, within Pf6N2E2_5674 at 6486.410 kb on - strand, within Pf6N2E2_5674 at 6486.410 kb on - strand, within Pf6N2E2_5674 at 6486.410 kb on - strand, within Pf6N2E2_5674 at 6486.436 kb on + strand, within Pf6N2E2_5674 at 6486.436 kb on + strand, within Pf6N2E2_5674 at 6486.436 kb on + strand, within Pf6N2E2_5674 at 6486.437 kb on - strand, within Pf6N2E2_5674 at 6486.437 kb on - strand, within Pf6N2E2_5674 at 6486.437 kb on - strand, within Pf6N2E2_5674 at 6486.442 kb on + strand, within Pf6N2E2_5674 at 6486.442 kb on + strand, within Pf6N2E2_5674 at 6486.442 kb on + strand, within Pf6N2E2_5674 at 6486.442 kb on + strand, within Pf6N2E2_5674 at 6486.442 kb on + strand, within Pf6N2E2_5674 at 6486.443 kb on - strand, within Pf6N2E2_5674 at 6486.443 kb on - strand, within Pf6N2E2_5674 at 6486.443 kb on - strand, within Pf6N2E2_5674 at 6486.443 kb on - strand, within Pf6N2E2_5674 at 6486.443 kb on - strand, within Pf6N2E2_5674 at 6486.625 kb on + strand, within Pf6N2E2_5674 at 6486.625 kb on + strand, within Pf6N2E2_5674 at 6486.626 kb on - strand, within Pf6N2E2_5674 at 6486.626 kb on - strand, within Pf6N2E2_5674 at 6486.626 kb on - strand, within Pf6N2E2_5674 at 6486.695 kb on - strand, within Pf6N2E2_5674 at 6486.901 kb on + strand, within Pf6N2E2_5674 at 6486.901 kb on + strand, within Pf6N2E2_5674 at 6486.901 kb on + strand, within Pf6N2E2_5674 at 6486.902 kb on - strand, within Pf6N2E2_5674 at 6486.986 kb on - strand, within Pf6N2E2_5674 at 6487.018 kb on + strand, within Pf6N2E2_5674 at 6487.018 kb on + strand, within Pf6N2E2_5674 at 6487.019 kb on - strand, within Pf6N2E2_5674 at 6487.019 kb on - strand, within Pf6N2E2_5674 at 6487.211 kb on + strand at 6487.249 kb on + strand at 6487.251 kb on + strand at 6487.251 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 6,485,185 - Pf6N2E2_5672 0.11 -0.1 6,485,369 + Pf6N2E2_5672 0.47 +1.7 6,485,369 + Pf6N2E2_5672 0.47 +0.7 6,485,369 + Pf6N2E2_5672 0.47 +1.7 6,485,370 - Pf6N2E2_5672 0.47 +0.5 6,485,370 - Pf6N2E2_5672 0.47 +0.2 6,485,370 - Pf6N2E2_5672 0.47 +0.1 6,485,370 - Pf6N2E2_5672 0.47 -1.1 6,485,370 - Pf6N2E2_5672 0.47 -0.3 6,485,487 + Pf6N2E2_5672 0.70 -0.3 6,485,696 + -1.5 6,485,761 + +0.6 6,485,761 + +1.7 6,485,761 + -1.1 6,485,761 + +0.1 6,485,762 - -0.8 6,485,848 + +0.7 6,485,884 + +1.2 6,485,884 + -0.6 6,485,884 + +1.7 6,485,884 + +0.7 6,485,885 - -1.2 6,485,947 + Pf6N2E2_5673 0.23 -0.5 6,485,947 + Pf6N2E2_5673 0.23 +0.3 6,485,947 + Pf6N2E2_5673 0.23 -0.3 6,485,947 + Pf6N2E2_5673 0.23 +1.7 6,485,947 + Pf6N2E2_5673 0.23 -0.6 6,485,948 - Pf6N2E2_5673 0.23 -0.2 6,485,948 - Pf6N2E2_5673 0.23 +1.7 6,485,948 - Pf6N2E2_5673 0.23 -0.2 6,485,948 - Pf6N2E2_5673 0.23 -0.5 6,486,084 + Pf6N2E2_5673 0.62 -0.7 6,486,084 + Pf6N2E2_5673 0.62 -0.4 6,486,084 + Pf6N2E2_5673 0.62 +1.1 6,486,085 - Pf6N2E2_5673 0.62 -0.8 6,486,107 - Pf6N2E2_5673 0.69 -1.7 6,486,127 + Pf6N2E2_5673 0.74 +0.1 6,486,127 + Pf6N2E2_5673 0.74 -0.6 6,486,128 - Pf6N2E2_5673 0.75 -0.1 6,486,128 - Pf6N2E2_5673 0.75 -0.0 6,486,128 - Pf6N2E2_5673 0.75 -0.3 6,486,271 + -1.4 6,486,271 + +0.4 6,486,272 - -0.6 6,486,366 + Pf6N2E2_5674 0.19 -0.4 6,486,367 - Pf6N2E2_5674 0.19 -0.8 6,486,409 + Pf6N2E2_5674 0.23 +0.4 6,486,409 + Pf6N2E2_5674 0.23 +0.3 6,486,410 - Pf6N2E2_5674 0.23 +0.4 6,486,410 - Pf6N2E2_5674 0.23 -2.2 6,486,410 - Pf6N2E2_5674 0.23 -1.4 6,486,410 - Pf6N2E2_5674 0.23 -1.4 6,486,410 - Pf6N2E2_5674 0.23 -0.7 6,486,410 - Pf6N2E2_5674 0.23 +0.4 6,486,436 + Pf6N2E2_5674 0.26 +0.7 6,486,436 + Pf6N2E2_5674 0.26 +0.3 6,486,436 + Pf6N2E2_5674 0.26 -3.0 6,486,437 - Pf6N2E2_5674 0.26 -0.3 6,486,437 - Pf6N2E2_5674 0.26 +1.7 6,486,437 - Pf6N2E2_5674 0.26 -1.3 6,486,442 + Pf6N2E2_5674 0.26 -0.7 6,486,442 + Pf6N2E2_5674 0.26 +0.7 6,486,442 + Pf6N2E2_5674 0.26 +0.4 6,486,442 + Pf6N2E2_5674 0.26 -0.9 6,486,442 + Pf6N2E2_5674 0.26 +0.0 6,486,443 - Pf6N2E2_5674 0.26 -0.9 6,486,443 - Pf6N2E2_5674 0.26 -1.0 6,486,443 - Pf6N2E2_5674 0.26 +0.7 6,486,443 - Pf6N2E2_5674 0.26 -0.4 6,486,443 - Pf6N2E2_5674 0.26 -0.8 6,486,625 + Pf6N2E2_5674 0.44 +0.7 6,486,625 + Pf6N2E2_5674 0.44 -0.2 6,486,626 - Pf6N2E2_5674 0.45 -2.0 6,486,626 - Pf6N2E2_5674 0.45 +0.7 6,486,626 - Pf6N2E2_5674 0.45 -1.4 6,486,695 - Pf6N2E2_5674 0.51 -0.3 6,486,901 + Pf6N2E2_5674 0.72 -0.8 6,486,901 + Pf6N2E2_5674 0.72 +0.6 6,486,901 + Pf6N2E2_5674 0.72 +0.1 6,486,902 - Pf6N2E2_5674 0.72 -0.9 6,486,986 - Pf6N2E2_5674 0.80 -1.0 6,487,018 + Pf6N2E2_5674 0.84 -0.6 6,487,018 + Pf6N2E2_5674 0.84 -1.6 6,487,019 - Pf6N2E2_5674 0.84 -0.5 6,487,019 - Pf6N2E2_5674 0.84 -2.3 6,487,211 + +0.3 6,487,249 + -0.0 6,487,251 + +0.6 6,487,251 + -0.8
Or see this region's nucleotide sequence