Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5674

Experiment: nitrogen source nitrite 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5672 and Pf6N2E2_5673 are separated by 228 nucleotidesPf6N2E2_5673 and Pf6N2E2_5674 overlap by 39 nucleotidesPf6N2E2_5674 and Pf6N2E2_5675 are separated by 146 nucleotides Pf6N2E2_5672 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 6,485,128 to 6,485,637 _5672 Pf6N2E2_5673 - hypothetical protein, at 6,485,866 to 6,486,216 _5673 Pf6N2E2_5674 - Low-specificity L-threonine aldolase (EC 4.1.2.48), at 6,486,178 to 6,487,182 _5674 Pf6N2E2_5675 - Alanyl-tRNA synthetase (EC 6.1.1.7), at 6,487,329 to 6,489,950 _5675 Position (kb) 6486 6487 6488Strain fitness (log2 ratio) -3 -2 -1 0 1at 6485.185 kb on - strand, within Pf6N2E2_5672at 6485.369 kb on + strand, within Pf6N2E2_5672at 6485.369 kb on + strand, within Pf6N2E2_5672at 6485.369 kb on + strand, within Pf6N2E2_5672at 6485.370 kb on - strand, within Pf6N2E2_5672at 6485.370 kb on - strand, within Pf6N2E2_5672at 6485.370 kb on - strand, within Pf6N2E2_5672at 6485.370 kb on - strand, within Pf6N2E2_5672at 6485.370 kb on - strand, within Pf6N2E2_5672at 6485.487 kb on + strand, within Pf6N2E2_5672at 6485.696 kb on + strandat 6485.761 kb on + strandat 6485.761 kb on + strandat 6485.761 kb on + strandat 6485.761 kb on + strandat 6485.762 kb on - strandat 6485.848 kb on + strandat 6485.884 kb on + strandat 6485.884 kb on + strandat 6485.884 kb on + strandat 6485.884 kb on + strandat 6485.885 kb on - strandat 6485.947 kb on + strand, within Pf6N2E2_5673at 6485.947 kb on + strand, within Pf6N2E2_5673at 6485.947 kb on + strand, within Pf6N2E2_5673at 6485.947 kb on + strand, within Pf6N2E2_5673at 6485.947 kb on + strand, within Pf6N2E2_5673at 6485.948 kb on - strand, within Pf6N2E2_5673at 6485.948 kb on - strand, within Pf6N2E2_5673at 6485.948 kb on - strand, within Pf6N2E2_5673at 6485.948 kb on - strand, within Pf6N2E2_5673at 6486.084 kb on + strand, within Pf6N2E2_5673at 6486.084 kb on + strand, within Pf6N2E2_5673at 6486.084 kb on + strand, within Pf6N2E2_5673at 6486.085 kb on - strand, within Pf6N2E2_5673at 6486.107 kb on - strand, within Pf6N2E2_5673at 6486.127 kb on + strand, within Pf6N2E2_5673at 6486.127 kb on + strand, within Pf6N2E2_5673at 6486.128 kb on - strand, within Pf6N2E2_5673at 6486.128 kb on - strand, within Pf6N2E2_5673at 6486.128 kb on - strand, within Pf6N2E2_5673at 6486.271 kb on + strandat 6486.271 kb on + strandat 6486.272 kb on - strandat 6486.366 kb on + strand, within Pf6N2E2_5674at 6486.367 kb on - strand, within Pf6N2E2_5674at 6486.409 kb on + strand, within Pf6N2E2_5674at 6486.409 kb on + strand, within Pf6N2E2_5674at 6486.410 kb on - strand, within Pf6N2E2_5674at 6486.410 kb on - strand, within Pf6N2E2_5674at 6486.410 kb on - strand, within Pf6N2E2_5674at 6486.410 kb on - strand, within Pf6N2E2_5674at 6486.410 kb on - strand, within Pf6N2E2_5674at 6486.410 kb on - strand, within Pf6N2E2_5674at 6486.436 kb on + strand, within Pf6N2E2_5674at 6486.436 kb on + strand, within Pf6N2E2_5674at 6486.436 kb on + strand, within Pf6N2E2_5674at 6486.437 kb on - strand, within Pf6N2E2_5674at 6486.437 kb on - strand, within Pf6N2E2_5674at 6486.437 kb on - strand, within Pf6N2E2_5674at 6486.442 kb on + strand, within Pf6N2E2_5674at 6486.442 kb on + strand, within Pf6N2E2_5674at 6486.442 kb on + strand, within Pf6N2E2_5674at 6486.442 kb on + strand, within Pf6N2E2_5674at 6486.442 kb on + strand, within Pf6N2E2_5674at 6486.443 kb on - strand, within Pf6N2E2_5674at 6486.443 kb on - strand, within Pf6N2E2_5674at 6486.443 kb on - strand, within Pf6N2E2_5674at 6486.443 kb on - strand, within Pf6N2E2_5674at 6486.443 kb on - strand, within Pf6N2E2_5674at 6486.625 kb on + strand, within Pf6N2E2_5674at 6486.625 kb on + strand, within Pf6N2E2_5674at 6486.626 kb on - strand, within Pf6N2E2_5674at 6486.626 kb on - strand, within Pf6N2E2_5674at 6486.626 kb on - strand, within Pf6N2E2_5674at 6486.695 kb on - strand, within Pf6N2E2_5674at 6486.901 kb on + strand, within Pf6N2E2_5674at 6486.901 kb on + strand, within Pf6N2E2_5674at 6486.901 kb on + strand, within Pf6N2E2_5674at 6486.902 kb on - strand, within Pf6N2E2_5674at 6486.986 kb on - strand, within Pf6N2E2_5674at 6487.018 kb on + strand, within Pf6N2E2_5674at 6487.018 kb on + strand, within Pf6N2E2_5674at 6487.019 kb on - strand, within Pf6N2E2_5674at 6487.019 kb on - strand, within Pf6N2E2_5674at 6487.211 kb on + strandat 6487.249 kb on + strandat 6487.251 kb on + strandat 6487.251 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM
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6,485,185 - Pf6N2E2_5672 0.11 -0.1
6,485,369 + Pf6N2E2_5672 0.47 +1.7
6,485,369 + Pf6N2E2_5672 0.47 +0.7
6,485,369 + Pf6N2E2_5672 0.47 +1.7
6,485,370 - Pf6N2E2_5672 0.47 +0.5
6,485,370 - Pf6N2E2_5672 0.47 +0.2
6,485,370 - Pf6N2E2_5672 0.47 +0.1
6,485,370 - Pf6N2E2_5672 0.47 -1.1
6,485,370 - Pf6N2E2_5672 0.47 -0.3
6,485,487 + Pf6N2E2_5672 0.70 -0.3
6,485,696 + -1.5
6,485,761 + +0.6
6,485,761 + +1.7
6,485,761 + -1.1
6,485,761 + +0.1
6,485,762 - -0.8
6,485,848 + +0.7
6,485,884 + +1.2
6,485,884 + -0.6
6,485,884 + +1.7
6,485,884 + +0.7
6,485,885 - -1.2
6,485,947 + Pf6N2E2_5673 0.23 -0.5
6,485,947 + Pf6N2E2_5673 0.23 +0.3
6,485,947 + Pf6N2E2_5673 0.23 -0.3
6,485,947 + Pf6N2E2_5673 0.23 +1.7
6,485,947 + Pf6N2E2_5673 0.23 -0.6
6,485,948 - Pf6N2E2_5673 0.23 -0.2
6,485,948 - Pf6N2E2_5673 0.23 +1.7
6,485,948 - Pf6N2E2_5673 0.23 -0.2
6,485,948 - Pf6N2E2_5673 0.23 -0.5
6,486,084 + Pf6N2E2_5673 0.62 -0.7
6,486,084 + Pf6N2E2_5673 0.62 -0.4
6,486,084 + Pf6N2E2_5673 0.62 +1.1
6,486,085 - Pf6N2E2_5673 0.62 -0.8
6,486,107 - Pf6N2E2_5673 0.69 -1.7
6,486,127 + Pf6N2E2_5673 0.74 +0.1
6,486,127 + Pf6N2E2_5673 0.74 -0.6
6,486,128 - Pf6N2E2_5673 0.75 -0.1
6,486,128 - Pf6N2E2_5673 0.75 -0.0
6,486,128 - Pf6N2E2_5673 0.75 -0.3
6,486,271 + -1.4
6,486,271 + +0.4
6,486,272 - -0.6
6,486,366 + Pf6N2E2_5674 0.19 -0.4
6,486,367 - Pf6N2E2_5674 0.19 -0.8
6,486,409 + Pf6N2E2_5674 0.23 +0.4
6,486,409 + Pf6N2E2_5674 0.23 +0.3
6,486,410 - Pf6N2E2_5674 0.23 +0.4
6,486,410 - Pf6N2E2_5674 0.23 -2.2
6,486,410 - Pf6N2E2_5674 0.23 -1.4
6,486,410 - Pf6N2E2_5674 0.23 -1.4
6,486,410 - Pf6N2E2_5674 0.23 -0.7
6,486,410 - Pf6N2E2_5674 0.23 +0.4
6,486,436 + Pf6N2E2_5674 0.26 +0.7
6,486,436 + Pf6N2E2_5674 0.26 +0.3
6,486,436 + Pf6N2E2_5674 0.26 -3.0
6,486,437 - Pf6N2E2_5674 0.26 -0.3
6,486,437 - Pf6N2E2_5674 0.26 +1.7
6,486,437 - Pf6N2E2_5674 0.26 -1.3
6,486,442 + Pf6N2E2_5674 0.26 -0.7
6,486,442 + Pf6N2E2_5674 0.26 +0.7
6,486,442 + Pf6N2E2_5674 0.26 +0.4
6,486,442 + Pf6N2E2_5674 0.26 -0.9
6,486,442 + Pf6N2E2_5674 0.26 +0.0
6,486,443 - Pf6N2E2_5674 0.26 -0.9
6,486,443 - Pf6N2E2_5674 0.26 -1.0
6,486,443 - Pf6N2E2_5674 0.26 +0.7
6,486,443 - Pf6N2E2_5674 0.26 -0.4
6,486,443 - Pf6N2E2_5674 0.26 -0.8
6,486,625 + Pf6N2E2_5674 0.44 +0.7
6,486,625 + Pf6N2E2_5674 0.44 -0.2
6,486,626 - Pf6N2E2_5674 0.45 -2.0
6,486,626 - Pf6N2E2_5674 0.45 +0.7
6,486,626 - Pf6N2E2_5674 0.45 -1.4
6,486,695 - Pf6N2E2_5674 0.51 -0.3
6,486,901 + Pf6N2E2_5674 0.72 -0.8
6,486,901 + Pf6N2E2_5674 0.72 +0.6
6,486,901 + Pf6N2E2_5674 0.72 +0.1
6,486,902 - Pf6N2E2_5674 0.72 -0.9
6,486,986 - Pf6N2E2_5674 0.80 -1.0
6,487,018 + Pf6N2E2_5674 0.84 -0.6
6,487,018 + Pf6N2E2_5674 0.84 -1.6
6,487,019 - Pf6N2E2_5674 0.84 -0.5
6,487,019 - Pf6N2E2_5674 0.84 -2.3
6,487,211 + +0.3
6,487,249 + -0.0
6,487,251 + +0.6
6,487,251 + -0.8

Or see this region's nucleotide sequence