Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_549 and Pf6N2E2_550 are separated by 1 nucleotides Pf6N2E2_550 and Pf6N2E2_551 overlap by 37 nucleotides Pf6N2E2_551 and Pf6N2E2_552 overlap by 17 nucleotides Pf6N2E2_552 and Pf6N2E2_553 are separated by 13 nucleotides
Pf6N2E2_549 - ClpB protein, at 619,344 to 621,887
_549
Pf6N2E2_550 - Uncharacterized protein ImpH/VasB, at 621,889 to 622,914
_550
Pf6N2E2_551 - Protein ImpG/VasA, at 622,878 to 624,668
_551
Pf6N2E2_552 - Uncharacterized protein ImpF, at 624,652 to 625,095
_552
Pf6N2E2_553 - Uncharacterized protein ImpD, at 625,109 to 625,606
_553
Position (kb) 622
623
624
625 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 622.061 kb on + strand, within Pf6N2E2_550 at 622.071 kb on - strand, within Pf6N2E2_550 at 622.520 kb on + strand, within Pf6N2E2_550 at 622.520 kb on + strand, within Pf6N2E2_550 at 622.520 kb on + strand, within Pf6N2E2_550 at 622.521 kb on - strand, within Pf6N2E2_550 at 622.521 kb on - strand, within Pf6N2E2_550 at 622.521 kb on - strand, within Pf6N2E2_550 at 622.620 kb on - strand, within Pf6N2E2_550 at 622.975 kb on + strand at 622.976 kb on - strand at 623.542 kb on + strand, within Pf6N2E2_551 at 623.575 kb on + strand, within Pf6N2E2_551 at 623.575 kb on + strand, within Pf6N2E2_551 at 623.576 kb on - strand, within Pf6N2E2_551 at 623.578 kb on + strand, within Pf6N2E2_551 at 623.815 kb on + strand, within Pf6N2E2_551 at 623.815 kb on + strand, within Pf6N2E2_551 at 623.815 kb on + strand, within Pf6N2E2_551 at 623.821 kb on + strand, within Pf6N2E2_551 at 623.821 kb on + strand, within Pf6N2E2_551 at 623.821 kb on + strand, within Pf6N2E2_551 at 623.896 kb on + strand, within Pf6N2E2_551 at 623.897 kb on - strand, within Pf6N2E2_551 at 623.925 kb on + strand, within Pf6N2E2_551 at 623.925 kb on + strand, within Pf6N2E2_551 at 623.926 kb on - strand, within Pf6N2E2_551 at 624.455 kb on - strand, within Pf6N2E2_551 at 624.631 kb on + strand at 624.631 kb on + strand at 624.631 kb on + strand at 624.631 kb on + strand at 624.632 kb on - strand at 624.635 kb on - strand at 624.674 kb on - strand at 624.757 kb on + strand, within Pf6N2E2_552 at 624.758 kb on - strand, within Pf6N2E2_552 at 624.917 kb on + strand, within Pf6N2E2_552 at 625.110 kb on + strand at 625.296 kb on + strand, within Pf6N2E2_553 at 625.297 kb on - strand, within Pf6N2E2_553 at 625.297 kb on - strand, within Pf6N2E2_553 at 625.297 kb on - strand, within Pf6N2E2_553 at 625.389 kb on + strand, within Pf6N2E2_553 at 625.518 kb on + strand, within Pf6N2E2_553 at 625.518 kb on + strand, within Pf6N2E2_553 at 625.519 kb on - strand, within Pf6N2E2_553 at 625.633 kb on + strand at 625.633 kb on + strand at 625.633 kb on + strand at 625.633 kb on + strand at 625.633 kb on + strand at 625.634 kb on - strand at 625.634 kb on - strand at 625.634 kb on - strand at 625.634 kb on - strand at 625.634 kb on - strand at 625.634 kb on - strand at 625.654 kb on - strand at 625.654 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 622,061 + Pf6N2E2_550 0.17 +1.3 622,071 - Pf6N2E2_550 0.18 +0.2 622,520 + Pf6N2E2_550 0.62 +1.7 622,520 + Pf6N2E2_550 0.62 +0.2 622,520 + Pf6N2E2_550 0.62 +0.4 622,521 - Pf6N2E2_550 0.62 +0.7 622,521 - Pf6N2E2_550 0.62 -0.6 622,521 - Pf6N2E2_550 0.62 -0.9 622,620 - Pf6N2E2_550 0.71 +0.2 622,975 + -0.8 622,976 - -0.0 623,542 + Pf6N2E2_551 0.37 +0.7 623,575 + Pf6N2E2_551 0.39 -0.3 623,575 + Pf6N2E2_551 0.39 +0.9 623,576 - Pf6N2E2_551 0.39 +0.4 623,578 + Pf6N2E2_551 0.39 -0.3 623,815 + Pf6N2E2_551 0.52 +0.7 623,815 + Pf6N2E2_551 0.52 -0.7 623,815 + Pf6N2E2_551 0.52 +0.3 623,821 + Pf6N2E2_551 0.53 +1.4 623,821 + Pf6N2E2_551 0.53 +1.7 623,821 + Pf6N2E2_551 0.53 +0.5 623,896 + Pf6N2E2_551 0.57 +0.5 623,897 - Pf6N2E2_551 0.57 +0.8 623,925 + Pf6N2E2_551 0.58 +1.9 623,925 + Pf6N2E2_551 0.58 -1.8 623,926 - Pf6N2E2_551 0.59 +1.7 624,455 - Pf6N2E2_551 0.88 +0.2 624,631 + +0.7 624,631 + +0.7 624,631 + +0.4 624,631 + +1.7 624,632 - +2.2 624,635 - +0.5 624,674 - -0.6 624,757 + Pf6N2E2_552 0.24 +2.3 624,758 - Pf6N2E2_552 0.24 +1.6 624,917 + Pf6N2E2_552 0.60 +1.7 625,110 + -1.6 625,296 + Pf6N2E2_553 0.38 -3.4 625,297 - Pf6N2E2_553 0.38 -1.9 625,297 - Pf6N2E2_553 0.38 +0.3 625,297 - Pf6N2E2_553 0.38 +1.8 625,389 + Pf6N2E2_553 0.56 -1.1 625,518 + Pf6N2E2_553 0.82 -0.1 625,518 + Pf6N2E2_553 0.82 +0.4 625,519 - Pf6N2E2_553 0.82 -2.2 625,633 + +0.8 625,633 + -2.2 625,633 + -2.2 625,633 + -1.2 625,633 + -0.4 625,634 - +1.8 625,634 - +0.5 625,634 - -1.7 625,634 - +0.1 625,634 - +0.2 625,634 - -0.2 625,654 - +0.8 625,654 - -2.7
Or see this region's nucleotide sequence