Experiment: nitrogen source nitrite 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5296 and Pf6N2E2_5297 are separated by 93 nucleotides Pf6N2E2_5297 and Pf6N2E2_5298 are separated by 30 nucleotides Pf6N2E2_5298 and Pf6N2E2_5299 overlap by 4 nucleotides
Pf6N2E2_5296 - Threonine synthase (EC 4.2.3.1), at 6,089,877 to 6,091,130
_5296
Pf6N2E2_5297 - RidA/YER057c/UK114 superfamily, group 2, YoaB-like protein, at 6,091,224 to 6,091,655
_5297
Pf6N2E2_5298 - Sarcosine oxidase beta subunit (EC 1.5.3.1), at 6,091,686 to 6,092,858
_5298
Pf6N2E2_5299 - Benzoate transport protein, at 6,092,855 to 6,094,060
_5299
Position (kb)
6091
6092
6093 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6090.865 kb on + strand, within Pf6N2E2_5296 at 6090.865 kb on + strand, within Pf6N2E2_5296 at 6090.934 kb on + strand, within Pf6N2E2_5296 at 6090.934 kb on + strand, within Pf6N2E2_5296 at 6090.934 kb on + strand, within Pf6N2E2_5296 at 6090.935 kb on - strand, within Pf6N2E2_5296 at 6090.935 kb on - strand, within Pf6N2E2_5296 at 6091.051 kb on + strand at 6091.051 kb on + strand at 6091.051 kb on + strand at 6091.051 kb on + strand at 6091.051 kb on + strand at 6091.051 kb on + strand at 6091.052 kb on - strand at 6091.052 kb on - strand at 6091.052 kb on - strand at 6091.052 kb on - strand at 6091.054 kb on + strand at 6091.054 kb on + strand at 6091.054 kb on + strand at 6091.054 kb on + strand at 6091.054 kb on + strand at 6091.054 kb on + strand at 6091.054 kb on + strand at 6091.054 kb on + strand at 6091.054 kb on + strand at 6091.055 kb on - strand at 6091.055 kb on - strand at 6091.055 kb on - strand at 6091.075 kb on + strand at 6091.075 kb on + strand at 6091.076 kb on - strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.117 kb on + strand at 6091.146 kb on + strand at 6091.147 kb on - strand at 6091.147 kb on - strand at 6091.319 kb on + strand, within Pf6N2E2_5297 at 6091.319 kb on - strand, within Pf6N2E2_5297 at 6091.319 kb on - strand, within Pf6N2E2_5297 at 6091.319 kb on - strand, within Pf6N2E2_5297 at 6091.519 kb on + strand, within Pf6N2E2_5297 at 6091.519 kb on + strand, within Pf6N2E2_5297 at 6091.519 kb on + strand, within Pf6N2E2_5297 at 6091.519 kb on + strand, within Pf6N2E2_5297 at 6091.520 kb on - strand, within Pf6N2E2_5297 at 6091.558 kb on + strand, within Pf6N2E2_5297 at 6091.558 kb on + strand, within Pf6N2E2_5297 at 6091.559 kb on - strand, within Pf6N2E2_5297 at 6091.559 kb on - strand, within Pf6N2E2_5297 at 6091.559 kb on - strand, within Pf6N2E2_5297 at 6091.559 kb on - strand, within Pf6N2E2_5297 at 6091.559 kb on - strand, within Pf6N2E2_5297 at 6091.559 kb on - strand, within Pf6N2E2_5297 at 6091.562 kb on + strand, within Pf6N2E2_5297 at 6091.563 kb on - strand, within Pf6N2E2_5297 at 6091.655 kb on + strand at 6091.656 kb on - strand at 6091.671 kb on + strand at 6091.671 kb on + strand at 6091.671 kb on + strand at 6091.671 kb on + strand at 6091.671 kb on + strand at 6091.671 kb on + strand at 6091.671 kb on + strand at 6091.672 kb on - strand at 6091.765 kb on + strand at 6091.886 kb on + strand, within Pf6N2E2_5298 at 6091.886 kb on + strand, within Pf6N2E2_5298 at 6092.140 kb on + strand, within Pf6N2E2_5298 at 6092.141 kb on - strand, within Pf6N2E2_5298 at 6092.181 kb on + strand, within Pf6N2E2_5298 at 6092.181 kb on + strand, within Pf6N2E2_5298 at 6092.395 kb on + strand, within Pf6N2E2_5298 at 6092.443 kb on + strand, within Pf6N2E2_5298 at 6092.443 kb on + strand, within Pf6N2E2_5298 at 6092.477 kb on - strand, within Pf6N2E2_5298 at 6092.613 kb on + strand, within Pf6N2E2_5298 at 6092.614 kb on - strand, within Pf6N2E2_5298 at 6092.614 kb on - strand, within Pf6N2E2_5298 at 6092.698 kb on + strand, within Pf6N2E2_5298 at 6092.741 kb on - strand at 6092.741 kb on - strand at 6092.797 kb on + strand at 6092.904 kb on + strand at 6092.904 kb on + strand at 6092.904 kb on + strand at 6092.905 kb on - strand at 6093.162 kb on + strand, within Pf6N2E2_5299 at 6093.162 kb on + strand, within Pf6N2E2_5299 at 6093.162 kb on + strand, within Pf6N2E2_5299 at 6093.162 kb on + strand, within Pf6N2E2_5299 at 6093.162 kb on + strand, within Pf6N2E2_5299 at 6093.162 kb on + strand, within Pf6N2E2_5299 at 6093.163 kb on - strand, within Pf6N2E2_5299 at 6093.163 kb on - strand, within Pf6N2E2_5299 at 6093.163 kb on - strand, within Pf6N2E2_5299 at 6093.163 kb on - strand, within Pf6N2E2_5299 at 6093.192 kb on + strand, within Pf6N2E2_5299 at 6093.192 kb on + strand, within Pf6N2E2_5299 at 6093.193 kb on - strand, within Pf6N2E2_5299 at 6093.193 kb on - strand, within Pf6N2E2_5299 at 6093.356 kb on + strand, within Pf6N2E2_5299 at 6093.375 kb on + strand, within Pf6N2E2_5299 at 6093.375 kb on + strand, within Pf6N2E2_5299 at 6093.376 kb on - strand, within Pf6N2E2_5299 at 6093.376 kb on - strand, within Pf6N2E2_5299 at 6093.631 kb on + strand, within Pf6N2E2_5299 at 6093.632 kb on - strand, within Pf6N2E2_5299 at 6093.765 kb on + strand, within Pf6N2E2_5299 at 6093.765 kb on + strand, within Pf6N2E2_5299 at 6093.765 kb on + strand, within Pf6N2E2_5299 at 6093.765 kb on + strand, within Pf6N2E2_5299 at 6093.765 kb on + strand, within Pf6N2E2_5299 at 6093.765 kb on + strand, within Pf6N2E2_5299 at 6093.765 kb on + strand, within Pf6N2E2_5299 at 6093.766 kb on - strand at 6093.766 kb on - strand, within Pf6N2E2_5299 at 6093.766 kb on - strand, within Pf6N2E2_5299 at 6093.815 kb on + strand, within Pf6N2E2_5299
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source nitrite 0.5mM remove 6,090,865 + Pf6N2E2_5296 0.79 +1.7 6,090,865 + Pf6N2E2_5296 0.79 +0.7 6,090,934 + Pf6N2E2_5296 0.84 -0.7 6,090,934 + Pf6N2E2_5296 0.84 -0.5 6,090,934 + Pf6N2E2_5296 0.84 -0.0 6,090,935 - Pf6N2E2_5296 0.84 +1.7 6,090,935 - Pf6N2E2_5296 0.84 -1.2 6,091,051 + +0.7 6,091,051 + +1.7 6,091,051 + +0.1 6,091,051 + -0.8 6,091,051 + -0.2 6,091,051 + -0.3 6,091,052 - +1.9 6,091,052 - +0.3 6,091,052 - -3.2 6,091,052 - -2.9 6,091,054 + +1.7 6,091,054 + +0.1 6,091,054 + -1.1 6,091,054 + -0.5 6,091,054 + -0.7 6,091,054 + +0.3 6,091,054 + +0.8 6,091,054 + -2.5 6,091,054 + -0.4 6,091,055 - +1.0 6,091,055 - +2.1 6,091,055 - +0.3 6,091,075 + +1.7 6,091,075 + +0.1 6,091,076 - -1.1 6,091,104 + -1.3 6,091,104 + -2.5 6,091,104 + -1.7 6,091,104 + +1.7 6,091,104 + +1.7 6,091,104 + +0.1 6,091,104 + -0.1 6,091,104 + -1.0 6,091,104 + -0.0 6,091,104 + +1.2 6,091,104 + +0.2 6,091,105 - +0.7 6,091,105 - +1.7 6,091,105 - +0.7 6,091,105 - -0.5 6,091,105 - -0.8 6,091,105 - +1.0 6,091,105 - -0.3 6,091,105 - +1.0 6,091,105 - -1.8 6,091,105 - +1.0 6,091,105 - +1.0 6,091,105 - +0.8 6,091,105 - -0.4 6,091,105 - -1.2 6,091,105 - +1.5 6,091,108 + -2.1 6,091,108 + -0.3 6,091,108 + +0.2 6,091,108 + +0.7 6,091,108 + +0.2 6,091,108 + -0.9 6,091,108 + -0.6 6,091,108 + +0.3 6,091,108 + -0.3 6,091,108 + +1.7 6,091,109 - -1.6 6,091,109 - -0.5 6,091,109 - +0.4 6,091,109 - -0.1 6,091,109 - +0.1 6,091,109 - +0.2 6,091,109 - +1.9 6,091,109 - +1.7 6,091,109 - -1.4 6,091,109 - -1.4 6,091,109 - +0.3 6,091,109 - +0.0 6,091,109 - -0.3 6,091,109 - -0.5 6,091,117 + -1.8 6,091,146 + -0.7 6,091,147 - -1.0 6,091,147 - -0.6 6,091,319 + Pf6N2E2_5297 0.22 -1.2 6,091,319 - Pf6N2E2_5297 0.22 -1.0 6,091,319 - Pf6N2E2_5297 0.22 -2.0 6,091,319 - Pf6N2E2_5297 0.22 +0.1 6,091,519 + Pf6N2E2_5297 0.68 +0.8 6,091,519 + Pf6N2E2_5297 0.68 +1.3 6,091,519 + Pf6N2E2_5297 0.68 -0.3 6,091,519 + Pf6N2E2_5297 0.68 -1.6 6,091,520 - Pf6N2E2_5297 0.69 +0.3 6,091,558 + Pf6N2E2_5297 0.77 +0.8 6,091,558 + Pf6N2E2_5297 0.77 -1.0 6,091,559 - Pf6N2E2_5297 0.78 +0.0 6,091,559 - Pf6N2E2_5297 0.78 +1.0 6,091,559 - Pf6N2E2_5297 0.78 -1.3 6,091,559 - Pf6N2E2_5297 0.78 +0.4 6,091,559 - Pf6N2E2_5297 0.78 -0.5 6,091,559 - Pf6N2E2_5297 0.78 +1.0 6,091,562 + Pf6N2E2_5297 0.78 -0.3 6,091,563 - Pf6N2E2_5297 0.78 +1.7 6,091,655 + +0.4 6,091,656 - -1.2 6,091,671 + -0.3 6,091,671 + -0.3 6,091,671 + -1.7 6,091,671 + +0.7 6,091,671 + +0.9 6,091,671 + -0.1 6,091,671 + -0.8 6,091,672 - +0.6 6,091,765 + +1.7 6,091,886 + Pf6N2E2_5298 0.17 +0.7 6,091,886 + Pf6N2E2_5298 0.17 -0.1 6,092,140 + Pf6N2E2_5298 0.39 -0.9 6,092,141 - Pf6N2E2_5298 0.39 -1.9 6,092,181 + Pf6N2E2_5298 0.42 +0.3 6,092,181 + Pf6N2E2_5298 0.42 -0.9 6,092,395 + Pf6N2E2_5298 0.60 -2.8 6,092,443 + Pf6N2E2_5298 0.65 -1.9 6,092,443 + Pf6N2E2_5298 0.65 -4.2 6,092,477 - Pf6N2E2_5298 0.67 -2.3 6,092,613 + Pf6N2E2_5298 0.79 -1.2 6,092,614 - Pf6N2E2_5298 0.79 -0.7 6,092,614 - Pf6N2E2_5298 0.79 +0.1 6,092,698 + Pf6N2E2_5298 0.86 -0.1 6,092,741 - +0.3 6,092,741 - -1.2 6,092,797 + +2.4 6,092,904 + +0.7 6,092,904 + -0.3 6,092,904 + +1.7 6,092,905 - +0.3 6,093,162 + Pf6N2E2_5299 0.25 -0.3 6,093,162 + Pf6N2E2_5299 0.25 -0.6 6,093,162 + Pf6N2E2_5299 0.25 -0.6 6,093,162 + Pf6N2E2_5299 0.25 -0.3 6,093,162 + Pf6N2E2_5299 0.25 -0.6 6,093,162 + Pf6N2E2_5299 0.25 +0.2 6,093,163 - Pf6N2E2_5299 0.26 +0.6 6,093,163 - Pf6N2E2_5299 0.26 +1.2 6,093,163 - Pf6N2E2_5299 0.26 +0.2 6,093,163 - Pf6N2E2_5299 0.26 +1.1 6,093,192 + Pf6N2E2_5299 0.28 -1.5 6,093,192 + Pf6N2E2_5299 0.28 +1.7 6,093,193 - Pf6N2E2_5299 0.28 +1.5 6,093,193 - Pf6N2E2_5299 0.28 -0.6 6,093,356 + Pf6N2E2_5299 0.42 -1.1 6,093,375 + Pf6N2E2_5299 0.43 -1.1 6,093,375 + Pf6N2E2_5299 0.43 -0.4 6,093,376 - Pf6N2E2_5299 0.43 -0.3 6,093,376 - Pf6N2E2_5299 0.43 -0.3 6,093,631 + Pf6N2E2_5299 0.64 +0.4 6,093,632 - Pf6N2E2_5299 0.64 -1.9 6,093,765 + Pf6N2E2_5299 0.75 +1.3 6,093,765 + Pf6N2E2_5299 0.75 -0.4 6,093,765 + Pf6N2E2_5299 0.75 +0.2 6,093,765 + Pf6N2E2_5299 0.75 +0.7 6,093,765 + Pf6N2E2_5299 0.75 -0.9 6,093,765 + Pf6N2E2_5299 0.75 -0.3 6,093,765 + Pf6N2E2_5299 0.75 +0.7 6,093,766 - +0.5 6,093,766 - Pf6N2E2_5299 0.76 -0.2 6,093,766 - Pf6N2E2_5299 0.76 -0.7 6,093,815 + Pf6N2E2_5299 0.80 +1.7
Or see this region's nucleotide sequence